Long-chain-fatty-acid--CoA ligase 2 (P39518)

Identification

Name

Long-chain-fatty-acid--CoA ligase 2

Synonyms

Fatty acid activator 2
Long-chain acyl-CoA synthetase 2

Gene Name

FAA2

Enzyme Class

6.2.1.3

Biological Properties

General Function

Involved in catalytic activity

Specific Function

Esterification, concomitant with transport, of endogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids. Preferentially acts on C9:0-C13:0 fatty acids although C7:0-C17:0 fatty acids are tolerated

Cellular Location

Cytoplasm. Mitochondrion.

SMPDB Pathways

Biosynthesis of unsaturated fatty acids	PW002403
Biosynthesis of unsaturated fatty acids (docosanoyl)	PW002408
Biosynthesis of unsaturated fatty acids (tetracosanoyl-CoA)	PW002404
Fatty acid metabolism	PW002460

KEGG Pathways

Biosynthesis of unsaturated fatty acids	ec01040
Fatty acid metabolism	ec00071

SMPDB Reactions

Palmitic acid + Adenosine triphosphate + Coenzyme A → Adenosine monophosphate + Pyrophosphate + Palmityl-CoA

KEGG Reactions

Adenosine triphosphate + myristic acid + Coenzyme A ↔ Adenosine monophosphate + Pyrophosphate + Tetradecanoyl-CoA

Adenosine triphosphate + Myristoleic acid + Coenzyme A ↔ Adenosine monophosphate + Pyrophosphate

Metabolites

YMDB ID	Name	View
YMDB00045	Coenzyme A	Show
YMDB00069	Palmitic acid	Show
YMDB00097	Adenosine monophosphate	Show
YMDB00109	Adenosine triphosphate	Show
YMDB00219	Pyrophosphate	Show
YMDB00301	Palmityl-CoA	Show
YMDB00469	hexadec-2-enoyl-CoA	Show
YMDB00527	palmitoyl-CoA	Show
YMDB00528	myristoyl-CoA	Show
YMDB00530	lauroyl-CoA	Show
YMDB00531	octanoyl-CoA	Show
YMDB00532	decanoyl-CoA	Show
YMDB00676	Caprylic acid	Show
YMDB00677	capric acid	Show
YMDB00678	lauric acid	Show
YMDB00679	myristic acid	Show
YMDB00680	Myristoleic acid	Show
YMDB00681	palmitoleic acid	Show

GO Classification

Component
Not Available
Function
catalytic activity
Process
metabolic process

Gene Properties

Chromosome Location

chromosome 5

Locus

YER015W

Gene Sequence

>2235 bp ATGGCCGCTCCAGATTATGCACTTACCGATTTAATTGAATCGGATCCTCGTTTCGAAAGT TTGAAGACAAGATTAGCCGGTTACACCAAAGGCTCTGATGAATATATTGAAGAGCTATAC TCTCAATTACCACTGACCAGCTATCCCAGGTACAAAACATTTTTAAAGAAACAGGCGGTT GCCATTTCGAATCCGGATAATGAAGCTGGTTTTAGCTCGATTTATAGGAGTTCTCTTTCT TCTGAAAATCTAGTGAGCTGTGTGGATAAAAACTTAAGAACTGCATACGATCACTTCATG TTTTCTGCAAGGAGATGGCCTCAACGTGACTGTTTAGGTTCAAGGCCAATTGATAAAGCC ACAGGCACCTGGGAGGAAACATTCCGTTTCGAGTCGTACTCCACGGTATCTAAAAGATGT CATAATATCGGAAGTGGTATATTGTCTTTGGTAAACACGAAAAGGAAACGTCCTTTGGAA GCCAATGATTTTGTTGTTGCTATCTTATCACACAACAACCCTGAATGGATCCTAACAGAT TTGGCCTGTCAGGCCTATTCTCTAACTAACACGGCTTTGTACGAAACATTAGGTCCAAAC ACCTCCGAGTACATATTGAATTTAACCGAGGCCCCCATTCTGATTTTTGCAAAATCAAAT ATGTATCATGTATTGAAGATGGTGCCTGATATGAAATTTGTTAATACTTTGGTTTGTATG GATGAATTAACTCATGACGAGCTCCGTATGCTAAATGAATCGTTGCTACCCGTTAAGTGC AACTCTCTCAATGAAAAAATCACATTTTTTTCATTGGAGCAGGTAGAACAAGTTGGTTGC TTTAACAAAATTCCTGCAATTCCACCTACCCCAGATTCCTTGTATACTATTTCGTTTACT TCTGGTACTACAGGTTTACCTAAAGGTGTGGAAATGTCTCACAGAAACATTGCGTCTGGG ATAGCATTTGCTTTTTCTACCTTCAGAATACCGCCAGATAAAAGAAACCAACAGTTATAT GATATGTGTTTTTTGCCATTGGCTCATATTTTTGAAAGAATGGTTATTGCGTATGATCTA GCCATCGGGTTTGGAATAGGCTTCTTACATAAACCAGACCCAACTGTATTGGTAGAGGAT TTGAAGATTTTGAAACCTTACGCGGTTGCCCTGGTTCCTAGAATATTAACACGGTTTGAA GCCGGTATAAAAAACGCTTTGGATAAATCGACTGTCCAGAGGAACGTAGCAAATACTATA TTGGATTCTAAATCGGCCAGATTTACCGCAAGAGGTGGTCCAGATAAATCGATTATGAAT TTTCTAGTTTATCATCGCGTATTGATTGATAAAATCAGAGACTCTTTAGGTTTGTCCAAT AACTCGTTTATAATTACCGGATCAGCTCCCATATCTAAAGATACCTTACTATTTTTAAGA AGTGCCTTGGATATTGGTATAAGACAGGGCTACGGCTTAACTGAAACTTTTGCTGGTGTC TGTTTAAGCGAACCGTTTGAAAAAGATGTCGGATCTTGTGGTGCCATAGGTATTTCTGCA GAATGTAGATTGAAGTCTGTTCCAGAAATGGGTTACCATGCCGACAAGGATTTAAAAGGT GAACTGCAAATTCGTGGCCCACAGGTTTTTGAAAGATATTTTAAAAATCCGAATGAAACT TCAAAAGCCGTTGACCAAGATGGTTGGTTTTCCACGGGAGATGTTGCATTTATCGATGGA AAAGGTCGCATCAGCGTCATTGATCGAGTCAAGAACTTTTTCAAGCTAGCACATGGTGAA TATATTGCTCCAGAGAAAATCGAAAATATTTATTTATCATCATGCCCCTATATCACGCAA ATATTTGTCTTTGGAGATCCTTTAAAGACATTTTTAGTTGGCATCGTTGGTGTTGATGTT GATGCAGCGCAACCGATTTTAGCTGCAAAGCACCCAGAGGTGAAAACGTGGACTAAGGAA GTGCTAGTAGAAAACTTAAATCGTAATAAAAAGCTAAGGAAGGAATTTTTAAACAAAATT AATAAATGCACCGATGGGCTACAAGGATTCGAAAAATTGCATAACATCAAAGTCGGACTT GAGCCTTTAACTCTCGAGGATGATGTTGTGACGCCAACTTTTAAAATAAAGCGTGCCAAA GCATCAAAATTCTTCAAAGATACATTAGACCAACTATACGCCGAAGGTTCACTAGTCAAG ACAGAAAAGCTTTAG

Protein Properties

Pfam Domain Function

AMP-binding (PF00501 )

Protein Residues

744

Protein Molecular Weight

83437.10156

Protein Theoretical pI

7.82

Signalling Regions

None

Transmembrane Regions

None

Protein Sequence

>Long-chain-fatty-acid--CoA ligase 2 MAAPDYALTDLIESDPRFESLKTRLAGYTKGSDEYIEELYSQLPLTSYPRYKTFLKKQAV AISNPDNEAGFSSIYRSSLSSENLVSCVDKNLRTAYDHFMFSARRWPQRDCLGSRPIDKA TGTWEETFRFESYSTVSKRCHNIGSGILSLVNTKRKRPLEANDFVVAILSHNNPEWILTD LACQAYSLTNTALYETLGPNTSEYILNLTEAPILIFAKSNMYHVLKMVPDMKFVNTLVCM DELTHDELRMLNESLLPVKCNSLNEKITFFSLEQVEQVGCFNKIPAIPPTPDSLYTISFT SGTTGLPKGVEMSHRNIASGIAFAFSTFRIPPDKRNQQLYDMCFLPLAHIFERMVIAYDL AIGFGIGFLHKPDPTVLVEDLKILKPYAVALVPRILTRFEAGIKNALDKSTVQRNVANTI LDSKSARFTARGGPDKSIMNFLVYHRVLIDKIRDSLGLSNNSFIITGSAPISKDTLLFLR SALDIGIRQGYGLTETFAGVCLSEPFEKDVGSCGAIGISAECRLKSVPEMGYHADKDLKG ELQIRGPQVFERYFKNPNETSKAVDQDGWFSTGDVAFIDGKGRISVIDRVKNFFKLAHGE YIAPEKIENIYLSSCPYITQIFVFGDPLKTFLVGIVGVDVDAAQPILAAKHPEVKTWTKE VLVENLNRNKKLRKEFLNKINKCTDGLQGFEKLHNIKVGLEPLTLEDDVVTPTFKIKRAK ASKFFKDTLDQLYAEGSLVKTEKL

References

External Links

Resource	Link
Saccharomyces Genome Database	FAA2
Uniprot ID	P39518
Uniprot Name	LCF2_YEAST
GenBank Gene ID	U18778
Genebank Protein ID	603607

General Reference

Johnson, D. R., Knoll, L. J., Rowley, N., Gordon, J. I. (1994). "Genetic analysis of the role of Saccharomyces cerevisiae acyl-CoA synthetase genes in regulating protein N-myristoylation." J Biol Chem 269:18037-18046.8027063
Harington, A., Schwarz, E., Slonimski, P. P., Herbert, C. J. (1994). "Subcellular relocalization of a long-chain fatty acid CoA ligase by a suppressor mutation alleviates a respiration deficiency in Saccharomyces cerevisiae." EMBO J 13:5531-5538.7988550
Dietrich, F. S., Mulligan, J., Hennessy, K., Yelton, M. A., Allen, E., Araujo, R., Aviles, E., Berno, A., Brennan, T., Carpenter, J., Chen, E., Cherry, J. M., Chung, E., Duncan, M., Guzman, E., Hartzell, G., Hunicke-Smith, S., Hyman, R. W., Kayser, A., Komp, C., Lashkari, D., Lew, H., Lin, D., Mosedale, D., Davis, R. W., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Nature 387:78-81.9169868
Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956