C-8 sterol isomerase (P32352)

Identification
NameC-8 sterol isomerase
Synonyms
  • Delta-8--delta-7 sterol isomerase
Gene NameERG2
Enzyme Class
Biological Properties
General FunctionInvolved in C-8 sterol isomerase activity
Specific FunctionCatalyzes the reaction which results in unsaturation at C-7 in the B ring of sterols
Cellular LocationEndoplasmic reticulum membrane; Single-pass membrane protein (Potential)
SMPDB Pathways
Cholesterol biosynthesis and metabolism CE(10:0)PW002545 ThumbThumb?image type=greyscaleThumb?image type=simple
Cholesterol biosynthesis and metabolism CE(12:0)PW002548 ThumbThumb?image type=greyscaleThumb?image type=simple
Cholesterol biosynthesis and metabolism CE(14:0)PW002544 ThumbThumb?image type=greyscaleThumb?image type=simple
Cholesterol biosynthesis and metabolism CE(16:0)PW002550 ThumbThumb?image type=greyscaleThumb?image type=simple
Cholesterol biosynthesis and metabolism CE(18:0)PW002551 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
Steroid biosynthesisec00100 Map00100
SMPDB Reactions
24-methylidene-5alpha-cholest-8-en-3beta-ol → episterol
zymosterol ↔ 5a-cholesta-7,24-dien-3B-ol
KEGG Reactions
24-methylidene-5alpha-cholest-8-en-3beta-ol → episterol
Metabolites
YMDB IDNameView
YMDB00540episterolShow
YMDB0054524-methylidene-5alpha-cholest-8-en-3beta-olShow
YMDB00548zymosterolShow
GO Classification
Component
organelle
membrane-bounded organelle
intracellular membrane-bounded organelle
endoplasmic reticulum
Function
catalytic activity
isomerase activity
intramolecular oxidoreductase activity
intramolecular oxidoreductase activity, transposing C=C bonds
C-8 sterol isomerase activity
Process
ergosterol biosynthetic process
metabolic process
small molecule metabolic process
alcohol metabolic process
sterol metabolic process
ergosterol metabolic process
Gene Properties
Chromosome Locationchromosome 13
LocusYMR202W
Gene Sequence>669 bp ATGAAGTTTTTCCCACTCCTTTTGTTGATTGGTGTTGTAGGCTACATTATGAACGTATTG TTCACTACCTGGTTGCCAACCAATTACATGTTCGATCCAAAAACTTTGAACGAAATATGT AACTCGGTGATTAGCAAACACAACGCAGCAGAAGGTTTATCCACTGAAGACCTGTTACAG GATGTCAGAGACGCACTTGCCTCTCATTACGGGGACGAATACATCAACAGGTACGTCAAA GAAGAATGGGTCTTCAACAATGCTGGTGGTGCGATGGGCCAAATGATCATCCTACACGCT TCCGTATCCGAGTACTTAATTCTATTCGGAACCGCTGTTGGTACTGAAGGGCACACAGGT GTTCACTTTGCTGACGACTATTTTACCATCTTACATGGTACGCAAATCGCAGCATTGCCA TATGCCACTGAAGCCGAAGTTTACACTCCTGGTATGACTCATCACTTGAAGAAGGGATAC GCCAAGCAATACAGCATGCCAGGTGGTTCCTTTGCCCTTGAATTGGCTCAAGGCTGGATT CCATGTATGTTGCCATTCGGGTTTTTGGACACTTTCTCCAGTACTCTTGATTTATACACT CTATATAGAACTGTCTACCTGACTGCCAGGGACATGGGTAAGAACTTGTTGCAAAACAAA AAGTTCTAA
Protein Properties
Pfam Domain Function
Protein Residues222
Protein Molecular Weight24895.40039
Protein Theoretical pI5.8
Signalling Regions
  • None
Transmembrane Regions
  • 3-23
Protein Sequence>C-8 sterol isomerase MKFFPLLLLIGVVGYIMNVLFTTWLPTNYMFDPKTLNEICNSVISKHNAAEGLSTEDLLQ DVRDALASHYGDEYINRYVKEEWVFNNAGGAMGQMIILHASVSEYLILFGTAVGTEGHTG VHFADDYFTILHGTQIAALPYATEAEVYTPGMTHHLKKGYAKQYSMPGGSFALELAQGWI PCMLPFGFLDTFSSTLDLYTLYRTVYLTARDMGKNLLQNKKF
References
External Links
ResourceLink
Saccharomyces Genome Database ERG2
Uniprot IDP32352
Uniprot NameERG2_YEAST
GenBank Gene IDAY558122
Genebank Protein ID45270134
General Reference
  • Arthington, B. A., Hoskins, J., Skatrud, P. L., Bard, M. (1991). "Nucleotide sequence of the gene encoding yeast C-8 sterol isomerase." Gene 107:173-174.1743517
  • Bowman, S., Churcher, C., Badcock, K., Brown, D., Chillingworth, T., Connor, R., Dedman, K., Devlin, K., Gentles, S., Hamlin, N., Hunt, S., Jagels, K., Lye, G., Moule, S., Odell, C., Pearson, D., Rajandream, M., Rice, P., Skelton, J., Walsh, S., Whitehead, S., Barrell, B. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Nature 387:90-93.9169872
  • Hu, Y., Rolfs, A., Bhullar, B., Murthy, T. V., Zhu, C., Berger, M. F., Camargo, A. A., Kelley, F., McCarron, S., Jepson, D., Richardson, A., Raphael, J., Moreira, D., Taycher, E., Zuo, D., Mohr, S., Kane, M. F., Williamson, J., Simpson, A., Bulyk, M. L., Harlow, E., Marsischky, G., Kolodner, R. D., LaBaer, J. (2007). "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Genome Res 17:536-543.17322287
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Natter, K., Leitner, P., Faschinger, A., Wolinski, H., McCraith, S., Fields, S., Kohlwein, S. D. (2005). "The spatial organization of lipid synthesis in the yeast Saccharomyces cerevisiae derived from large scale green fluorescent protein tagging and high resolution microscopy." Mol Cell Proteomics 4:662-672.15716577