Probable phospholipid-transporting ATPase DNF2 (Q12675)

Identification

Name

Probable phospholipid-transporting ATPase DNF2

Synonyms

Not Available

Gene Name

DNF2

Enzyme Class

3.6.3.1

Biological Properties

General Function

Involved in nucleotide binding

Specific Function

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of phospholipids. Required for protein transport from Golgi to vacuoles

Cellular Location

Cell membrane; Multi-pass membrane protein

SMPDB Pathways

Lysolipid incorporation into Mitochondria	PW002531
Lysolipid incorporation into Mitochondria PC(10:0/10:0)	PW002791
Lysolipid incorporation into Mitochondria PC(14:0/14:0)	PW002792
Lysolipid incorporation into Mitochondria PC(16:0/16:0)	PW002753
Lysolipid incorporation into Mitochondria PC(16:1(11Z)/16:1(11Z))	PW002794

KEGG Pathways

Not Available

SMPDB Reactions

Adenosine triphosphate + water + PC(14:0/0:0) → ADP + phosphate + PC(14:0/0:0)

Adenosine triphosphate + water + PE(14:0/0:0) → ADP + phosphate + PE(14:0/0:0)

Adenosine triphosphate + water + Lyso-PS(14:0/0:0) → ADP + phosphate + Lyso-PS(14:0/0:0)

Lyso-PI(14:0/0:0) + Adenosine triphosphate + water → Lyso-PI(14:0/0:0) + ADP + phosphate

Lyso-PS(0:0/14:0) + Adenosine triphosphate + water → Lyso-PS(0:0/14:0) + ADP + phosphate

KEGG Reactions

Not Available

Metabolites

YMDB ID	Name	View
YMDB00109	Adenosine triphosphate	Show
YMDB00890	water	Show
YMDB00907	phosphate	Show
YMDB00914	ADP	Show
YMDB01175	PC(14:0/0:0)	Show
YMDB01194	PE(14:0/0:0)	Show
YMDB13347	Lyso-PI(0:0/14:0)	Show
YMDB13365	Lyso-PS(0:0/14:0)	Show

GO Classification

Component
cell part
membrane
membrane part
intrinsic to membrane
integral to membrane
Function
purine nucleoside binding
ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism
adenyl nucleotide binding
hydrolase activity, acting on acid anhydrides
adenyl ribonucleotide binding
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances
ATP binding
ion binding
cation binding
metal ion binding
substrate-specific transporter activity
transporter activity
lipid transporter activity
transmembrane transporter activity
phospholipid transporter activity
phospholipid-translocating ATPase activity
hydrolase activity
substrate-specific transmembrane transporter activity
ion transmembrane transporter activity
binding
magnesium ion binding
nucleoside binding
ATPase activity, coupled to transmembrane movement of ions
catalytic activity
Process
metabolic process
purine nucleoside triphosphate biosynthetic process
purine ribonucleoside triphosphate biosynthetic process
ATP biosynthetic process
nitrogen compound metabolic process
cellular nitrogen compound metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleobase, nucleoside and nucleotide metabolic process
establishment of localization
nucleoside phosphate metabolic process
transport
lipid transport
nucleotide metabolic process
phospholipid transport
purine nucleotide metabolic process
purine nucleotide biosynthetic process

Gene Properties

Chromosome Location

chromosome 4

Locus

YDR093W

Gene Sequence

>4839 bp ATGTCAAGTCCCTCCAAACCCACTTCTCCCTTCGTGGATGATATTGAGCATGAATCGGGA TCAGCATCTAATGGTCTGTCGTCCATGTCACCATTTGACGATAGTTTTCAATTTGAAAAA CCCAGTAGTGCGCATGGAAATATTGAAGTAGCGAAAACCGGCGGTTCTGTTTTGAAGCGA CAATCTAAGCCAATGAAAGATATTAGCACACCCGATCTCTCAAAAGTTACTTTTGATGGA ATCGATGATTATAGTAACGATAATGATATTAATGATGATGATGAACTCAATGGTAAAAAA ACCGAGATACATGAACACGAAAATGAGGTGGATGATGACCTCCATTCGTTCCAGGCAACA CCTATGCCTAATACTGGAGGCTTTGAGGATGTTGAGTTGGATAACAATGAAGGTAGCAAC AATGATTCCCAGGCAGATCATAAGTTGAAAAGAGTACGTTTTGGTACAAGAAGAAATAAG TCAGGTAGGATAGATATAAATAGGTCAAAAACATTGAAATGGGCCAAGAAGAACTTCCAC AATGCTATTGATGAATTCAGTACAAAAGAAGACAGTCTCGAAAATTCAGCCTTGCAAAAT AGGTCTGATGAGTTGAGAACAGTTTACTATAATTTACCCTTGCCGGAGGACATGCTTGAC GAGGACGGCTTACCGTTGGCAGTTTACCCGCGTAATAAAATCAGGACTACGAAGTATACT CCATTAACATTTTTTCCCAAGAACATTCTTTTCCAATTTCATAATTTTGCCAATATTTAC TTTTTAATACTGTTGATTCTAGGTGCTTTTCAAATATTTGGTGTGACAAATCCAGGTTTT GCATCCGTTCCCCTAATTGTCATCGTAATTATCACTGCTATTAAGGATGGTATCGAAGAT TCCAGAAGGACTGTATTGGATTTAGAAGTTAATAATACAAGAACACATATCTTAAGCGGG GTGAAGAATGAAAATGTTGCTGTTGATAACGTTTCCTTATGGAGGCGTTTCAAGAAAGCG AATACAAGGGCATTGATTAAAATTTTTGAGTATTTTTCAGAAAACCTCACTGCTGCTGGT AGAGAAAAGAAGTTACAGAAAAAAAGGGAAGAATTACGAAGGAAGAGGAACTCGAGAAGC TTTGGGCCACGTGGGTCTCTGGATTCAATTGGTAGTTACAGAATGTCTGCCGACTTTGGT CGTCCCTCTTTGGATTATGAGAACCTGAATCAAACAATGTCACAGGCTAACAGGTATAAC GATGGTGAAAATTTAGTTGATCGAACGCTACAGCCAAACCCTGAATGTAGGTTTGCCAAG GATTACTGGAAAAATGTTAAAGTTGGTGATATTGTTCGTGTTCACAATAACGATGAAATT CCTGCTGATATGATCTTATTATCGACATCAGATGTTGATGGCGCTTGTTATGTGGAAACC AAAAATTTGGATGGTGAAACAAATCTAAAGGTCCGTCAATCATTAAAATGTAGCAAGATT ATTAAGAGTTCAAGGGATATTACCAGGACGAAGTTTTGGGTTGAAAGTGAAGGCCCACAT GCCAACTTGTATTCTTATCAAGGTAATTTCAAATGGCAAGATACACAAAATGGAAACATC AGAAATGAGCCTGTAAATATCAATAACTTATTATTGAGAGGTTGTACCTTGAGAAACACT AAGTGGGCCATGGGTATGGTTATTTTTACTGGTGACGATACCAAGATTATGATAAACGCT GGTGTCACTCCAACCAAAAAATCCAGAATTTCAAGAGAGTTGAATTTTTCTGTGATTTTG AATTTTGTACTATTGTTTATCCTATGTTTCACTGCTGGTATTGTTAACGGTGTTTATTAT AAACAGAAACCTCGATCGAGAGATTATTTTGAATTTGGTACCATTGGAGGTTCTGCCTCA ACTAACGGTTTTGTTTCTTTTTGGGTGGCGGTTATTCTTTATCAGTCATTGGTTCCGATC TCTTTATATATTTCTGTGGAAATTATTAAGACAGCACAGGCTATTTTCATTTACACTGAT GTTTTGTTGTATAATGCCAAGCTAGACTATCCATGTACACCAAAGTCGTGGAACATCTCC GATGATTTGGGTCAAATTGAGTATATTTTTTCAGATAAAACAGGCACCTTGACACAAAAT GTGATGGAATTCAAAAAATGTACTATTAACGGTGTGTCTTATGGACGTGCCTATACGGAG GCACTAGCAGGCCTGAGGAAGAGACAAGGTGTTGATGTCGAATCGGAAGGTCGTCGCGAA AAGGAAGAGATAGCAAAAGATAGGGAAACAATGATCGACGAATTAAGATCAATGAGTGAT AATACACAGTTTTGTCCGGAGGATTTAACTTTTGTATCTAAGGAAATTGTGGAAGACTTG AAAGGATCCAGTGGCGACCATCAACAAAAGTGCTGTGAACACTTTTTGCTGGCGCTCGCA TTATGTCATTCTGTCCTTGTGGAGCCAAATAAAGATGATCCTAAAAAACTGGACATCAAG GCTCAATCACCTGATGAGTCTGCCTTAGTTTCCACCGCCAGACAATTAGGGTACAGTTTC GTTGGTAGTTCAAAAAGTGGATTGATAGTAGAGATCCAGGGTGTTCAAAAGGAGTTCCAA GTTCTGAACGTACTTGAATTCAATTCGTCGAGAAAAAGGATGAGTTGCATTATCAAGATC CCTGGCTCTACACCAAAGGATGAACCTAAAGCACTGTTAATATGTAAAGGTGCGGATTCC GTTATCTATTCAAGATTAGATCGCACTCAAAACGACGCTACTTTGTTGGAAAAGACGGCC CTCCATTTGGAAGAATATGCCACAGAAGGTTTAAGAACATTATGTTTGGCTCAAAGAGAG CTCACGTGGTCCGAGTATGAACGTTGGGTTAAGACTTATGATGTGGCCGCTGCATCCGTT ACTAATAGGGAGGAAGAGTTGGACAAAGTTACTGACGTTATTGAACGTGAGCTTATTTTG CTTGGTGGTACGGCCATTGAGGATCGTTTACAAGATGGTGTTCCTGATTCGATTGCCCTC TTGGCCGAGGCCGGTATTAAGTTATGGGTCCTAACGGGTGATAAAGTGGAAACTGCCATT AACATTGGTTTTTCCTGCAATGTTTTGAACAATGATATGGAATTATTGGTTGTGAAGGCT TCCGGCGAAGATGTAGAAGAATTTGGTAGTGACCCTATTCAAGTGGTTAACAATTTGGTG ACAAAATACTTAAGGGAAAAATTTGGCATGAGTGGATCCGAGGAGGAGTTAAAAGAAGCT AAAAGAGAGCATGGTTTGCCACAGGGAAATTTTGCTGTCATTATCGATGGCGATGCGCTA AAAGTTGCTTTGAATGGTGAAGAAATGAGACGCAAGTTCTTGCTACTGTGTAAGAATTGT AAAGCTGTTTTGTGTTGCAGAGTTTCTCCAGCACAAAAGGCTGCAGTGGTCAAGTTGGTA AAAAAGACCTTAGACGTTATGACCTTGGCCATTGGTGATGGTTCGAATGATGTAGCCATG ATTCAATCGGCGGACGTTGGTGTTGGTATCGCGGGTGAAGAAGGCAGACAAGCTGTCATG TGTTCTGATTATGCCATCGGGCAGTTCAGATATGTGACAAGACTGGTATTGGTGCATGGT AAATGGTGTTATAAGAGACTGGCAGAGATGATCCCACAGTTTTTCTACAAGAACGTTATT TTTACGCTATCCCTGTTCTGGTATGGTATTTACAATAATTTCGATGGGTCGTATTTGTTT GAGTATACGTACTTGACATTCTACAATCTTGCATTCACATCCGTACCTGTTATTTTACTA GCAGTACTCGACCAAGATGTTTCCGACACCGTTTCGATGCTGGTGCCACAGTTGTACCGT GTGGGTATCCTAAGAAAAGAATGGAACCAAACCAAATTTCTTTGGTACATGTTGGATGGT GTATACCAATCTGTGATATGCTTCTTTTTCCCCTATTTGGCGTACCATAAAAACATGGTT GTAACTGAAAATGGCCTTGGACTAGACCACCGTTACTTTGTCGGTGTCTTCGTTACAGCA ATTGCAGTCACTTCATGTAACTTTTACGTCTTCATGGAACAGTATAGATGGGACTGGTTC TGTGGCCTATTCATATGTCTATCACTGGCCGTGTTTTACGGCTGGACGGGTATATGGACA AGCTCGTCGTCAAGTAACGAATTTTACAAGGGTGCGGCGCGTGTATTCGCACAACCTGCC TATTGGGCAGTACTTTTCGTTGGCGTGCTGTTCTGCCTACTGCCAAGATTCACCATTGAC TGTATCAGAAAGATATTCTATCCGAAAGATATAGAGATCGTTAGAGAAATGTGGTTACGC GGTGATTTTGACCTCTACCCACAAGGCTACGACCCTACGGACCCTAGCAGGCCCAGAATA AACGAAATACGTCCCCTTACAGATTTCAAAGAGCCCATTTCACTGGACACTCATTTTGAT GGTGTCAGCCACTCACAAGAGACCATAGTCACAGAGGAAATACCAATGAGTATACTCAAT GGTGAGCAAGGCTCGCGCAAGGGGTACCGTGTCTCTACCACTCTGGAACGCCGTGACCAG CTCAGCCCCGTTACAACAACGAACAACTTGCCTAGAAGATCAATGGCCAGTGCCCGCGGA AATAAGCTCAGAACTTCGCTGGATCGCACAAGAGAAGAGATGCTTGCCAACCACCAACTG GACACAAGATACTCCGTGGAAAGAGCACGTGCCTCGCTGGATCTGCCGGGTATCAATCAT GCAGAGACGCTGCTCAGCCAGCGCAGCAGGGACCGTTAA

Protein Properties

Pfam Domain Function

E1-E2_ATPase (PF00122 )
Hydrolase (PF00702 )

Protein Residues

1612

Protein Molecular Weight

182617.0

Protein Theoretical pI

6.04

Signalling Regions

None

Transmembrane Regions

253-273
278-298
599-619
640-660
1232-1252
1263-1283
1314-1334
1351-1371
1378-1398
1419-1439

Protein Sequence

>Probable phospholipid-transporting ATPase DNF2 MSSPSKPTSPFVDDIEHESGSASNGLSSMSPFDDSFQFEKPSSAHGNIEVAKTGGSVLKR QSKPMKDISTPDLSKVTFDGIDDYSNDNDINDDDELNGKKTEIHEHENEVDDDLHSFQAT PMPNTGGFEDVELDNNEGSNNDSQADHKLKRVRFGTRRNKSGRIDINRSKTLKWAKKNFH NAIDEFSTKEDSLENSALQNRSDELRTVYYNLPLPEDMLDEDGLPLAVYPRNKIRTTKYT PLTFFPKNILFQFHNFANIYFLILLILGAFQIFGVTNPGFASVPLIVIVIITAIKDGIED SRRTVLDLEVNNTRTHILSGVKNENVAVDNVSLWRRFKKANTRALIKIFEYFSENLTAAG REKKLQKKREELRRKRNSRSFGPRGSLDSIGSYRMSADFGRPSLDYENLNQTMSQANRYN DGENLVDRTLQPNPECRFAKDYWKNVKVGDIVRVHNNDEIPADMILLSTSDVDGACYVET KNLDGETNLKVRQSLKCSKIIKSSRDITRTKFWVESEGPHANLYSYQGNFKWQDTQNGNI RNEPVNINNLLLRGCTLRNTKWAMGMVIFTGDDTKIMINAGVTPTKKSRISRELNFSVIL NFVLLFILCFTAGIVNGVYYKQKPRSRDYFEFGTIGGSASTNGFVSFWVAVILYQSLVPI SLYISVEIIKTAQAIFIYTDVLLYNAKLDYPCTPKSWNISDDLGQIEYIFSDKTGTLTQN VMEFKKCTINGVSYGRAYTEALAGLRKRQGVDVESEGRREKEEIAKDRETMIDELRSMSD NTQFCPEDLTFVSKEIVEDLKGSSGDHQQKCCEHFLLALALCHSVLVEPNKDDPKKLDIK AQSPDESALVSTARQLGYSFVGSSKSGLIVEIQGVQKEFQVLNVLEFNSSRKRMSCIIKI PGSTPKDEPKALLICKGADSVIYSRLDRTQNDATLLEKTALHLEEYATEGLRTLCLAQRE LTWSEYERWVKTYDVAAASVTNREEELDKVTDVIERELILLGGTAIEDRLQDGVPDSIAL LAEAGIKLWVLTGDKVETAINIGFSCNVLNNDMELLVVKASGEDVEEFGSDPIQVVNNLV TKYLREKFGMSGSEEELKEAKREHGLPQGNFAVIIDGDALKVALNGEEMRRKFLLLCKNC KAVLCCRVSPAQKAAVVKLVKKTLDVMTLAIGDGSNDVAMIQSADVGVGIAGEEGRQAVM CSDYAIGQFRYVTRLVLVHGKWCYKRLAEMIPQFFYKNVIFTLSLFWYGIYNNFDGSYLF EYTYLTFYNLAFTSVPVILLAVLDQDVSDTVSMLVPQLYRVGILRKEWNQTKFLWYMLDG VYQSVICFFFPYLAYHKNMVVTENGLGLDHRYFVGVFVTAIAVTSCNFYVFMEQYRWDWF CGLFICLSLAVFYGWTGIWTSSSSSNEFYKGAARVFAQPAYWAVLFVGVLFCLLPRFTID CIRKIFYPKDIEIVREMWLRGDFDLYPQGYDPTDPSRPRINEIRPLTDFKEPISLDTHFD GVSHSQETIVTEEIPMSILNGEQGSRKGYRVSTTLERRDQLSPVTTTNNLPRRSMASARG NKLRTSLDRTREEMLANHQLDTRYSVERARASLDLPGINHAETLLSQRSRDR

References

External Links

Resource	Link
Saccharomyces Genome Database	DNF2
Uniprot ID	Q12675
Uniprot Name	ATC4_YEAST
GenBank Gene ID	Z47746
Genebank Protein ID	633628

General Reference

Jacq, C., Alt-Morbe, J., Andre, B., Arnold, W., Bahr, A., Ballesta, J. P., Bargues, M., Baron, L., Becker, A., Biteau, N., Blocker, H., Blugeon, C., Boskovic, J., Brandt, P., Bruckner, M., Buitrago, M. J., Coster, F., Delaveau, T., del Rey, F., Dujon, B., Eide, L. G., Garcia-Cantalejo, J. M., Goffeau, A., Gomez-Peris, A., Zaccaria, P., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Nature 387:75-78.9169867
Hua, Z., Fatheddin, P., Graham, T. R. (2002). "An essential subfamily of Drs2p-related P-type ATPases is required for protein trafficking between Golgi complex and endosomal/vacuolar system." Mol Biol Cell 13:3162-3177.12221123
Gruhler, A., Olsen, J. V., Mohammed, S., Mortensen, P., Faergeman, N. J., Mann, M., Jensen, O. N. (2005). "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Mol Cell Proteomics 4:310-327.15665377
Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258
Li, X., Gerber, S. A., Rudner, A. D., Beausoleil, S. A., Haas, W., Villen, J., Elias, J. E., Gygi, S. P. (2007). "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." J Proteome Res 6:1190-1197.17330950
Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956