Identification |
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Name | Bifunctional protein RIB2 |
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Synonyms | - tRNA pseudouridine synthase 8, cytoplasmic
- tRNA pseudouridylate synthase 8
- tRNA-uridine isomerase 8
- Diaminohydroxyphosphoribosylaminopyrimidine deaminase
- DRAP deaminase
- Riboflavin-specific deaminase
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Gene Name | RIB2 |
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Enzyme Class | |
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Biological Properties |
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General Function | Involved in zinc ion binding |
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Specific Function | Involved in riboflavin biosynthesis. Converts 2,5- diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate into 5- amino-6-(ribosylamino)-2,4(1H,3H)-pyrimidinedione 5'-phosphate |
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Cellular Location | Cytoplasm |
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SMPDB Pathways | |
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KEGG Pathways | |
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SMPDB Reactions | |
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KEGG Reactions | |
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Metabolites | YMDB ID | Name | View |
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YMDB00091 | Ammonia | Show | YMDB00147 | 2,5-Diamino-4-hydroxy-6-(5-phosphoribosylamino)pyrimidine | Show | YMDB00163 | 5-Amino-6-(5'-phosphoribosylamino)uracil | Show | YMDB00423 | Ammonium | Show | YMDB00424 | 2,5-Diamino-6-(5-phosphono)ribitylamino-4(3H)-pyrimidinone | Show | YMDB00425 | 5-Amino-6-(5-phospho-D-ribitylamino)uracil | Show | YMDB00862 | hydron | Show | YMDB00890 | water | Show | YMDB16310 | 2,5-Diamino-6-(1-D-ribitylamino)pyrimidin-4(3H)-one 5'-phosphate | Show | YMDB16311 | 5-Amino-2,6-dioxy-4-(5'-phospho-D-ribitylamino)pyrimidine | Show |
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GO Classification | Component |
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Not Available | Function |
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catalytic activity | pseudouridine synthase activity | RNA binding | binding | ion binding | cation binding | metal ion binding | transition metal ion binding | zinc ion binding | hydrolase activity | isomerase activity | intramolecular transferase activity | nucleic acid binding | Process |
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metabolic process | macromolecule metabolic process | RNA modification | pseudouridine synthesis | cellular macromolecule metabolic process | RNA metabolic process |
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Gene Properties |
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Chromosome Location | chromosome 15 |
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Locus | YOL066C |
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Gene Sequence | >1776 bp
ATGGAGGACTCTAATAATGAAGCCAGCGATGACTTCAACAACTTATTAAACAAAGAAATT
GAGTCTGCAAAGGAAGTGAAGTTAAGAAAATTCGCGAACAGGAATAATAATAGGAACGAA
AACAGTTCCAAAGTCAAAGATGCGAGTGGATTCAGACTGAGAGTTATTCAAACCGATGGG
CACAAAACTAAAAAGACAGACCCTGATTATGAAGTGACAATTGATGGACCCTTAAGGAAG
ATTGAGCCTTATTTTTTCACTTATAAAACTTTCTGTAAGGAGAGGTGGAGAGATCGTAAG
TTGGTGGATGTATTCGTGAGCGAATTCAGGGATCGAGAGCCCAGCTATTATTCCAAAACC
ATTGCAGAGGGAAAAGTATACTTAAACGATGAACCTGCAAACCTTGATACCATCATTCGT
GACGGTGATCTGATCACACATAAAGTACATCGACATGAACCGCCAGTCACATCTAAACCA
ATAGATATTGTGTTTGAAGACGAAGATATCCTTGTCATTGATAAACCCAGCAGTATTCCA
GTGCACCCAACGGGCAGATACAGATTCAATACCATTACAAAAATGCTAGAAAGACAGCTA
GGTTATTCAGTACATCCATGTAATAGACTAGACAAGCCAACTAGTGGACTAATGTTTTTA
GCCAAGACTCCATTAGGTGCAGATAGAATGGGAGACCAAATGAAAGCGAGGGAAGTCACC
AAAGAATACGTAGCCCGCGTGAAGGGCGAGTTCCCTATAGGTATAGTAGAGGTAGATAAG
CCTGTCAGATCTGTTAACCCTAAAGTTGCACTAAACGCTGTTTGCGAGATGAGCGATGAA
AACGCTAAACATGCCAAGACCGTTTTCCAAAGGGTTAGCTATGATGGACAGACGAGTATT
GTAAAGTGTAAACCATTGACAGGTAGAACTCACCAAATAAGAGTCCATTTGCAGTACTTG
GGATTTCCCATAGCAAATGACCCTATATACTCGAACCCAGATATCTGGGGTCCAGATTTG
GGCCGCGGTGGACTTCAAAACTATGATGACATCGTTCTGAAACTAGACGCTATTGGCAAG
ACTAATCCTGCAGAGAGCTGGATTCATCCTCATAGCGAGGGTGAATACTTGCTTGGCCGT
CAGTGCGAAGAATGCGAGGCTGAAATGTACACAGATCCCGGTACTAATGATCTCGACCTC
TGGCTGCATGCCTTCCGGTACGAGTCATTGGAAAGAAATTCGGATACGCAAAAACCTCTC
TGGAGTTATAGAACAAAATACCCTGAATGGGCCTTAGAGCCACATCGCCGATATATGGAA
ATGGCCGTTAAGGAAGCTGGTAAGTGTGGCCCGACAAAGACTGCTTTTAGTGTTGGTGCC
GTTCTTGTTCATGGAACTCAAGTACTTGCCACAGGATATTCAAGAGAGCTACCAGGAAAC
ACTCATGCAGAACAGTGTGCTTTGATAAAGTACTCGCAGTTACACCCGAACTGTCCCACT
ATAGTCCCTATGGGAACAGTGCTCTATACAACCATGGAACCCTGTTCCTTCAGGCTAAGT
GGCAATGAGCCTTGCTGCGATAGAATCCTGGCCACCCAAGGTGCCATTGGTACTGTCTTC
GTGGGGGTTATGGAGCCCGATACATTTGTTAAAAACAATACAAGTTTGAACAAGCTGGAA
TCGCACGGTGTGAACTACATACAAATACCAGGCTACGAGGAGGAGTGCACCATCATTGCC
TTCAAAGGCCACGATAATAGTGACGACAAAGCTTAG |
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Protein Properties |
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Pfam Domain Function | |
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Protein Residues | 591 |
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Protein Molecular Weight | 67035.29688 |
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Protein Theoretical pI | 6.51 |
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Signalling Regions | |
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Transmembrane Regions | |
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Protein Sequence | >Bifunctional protein RIB2
MEDSNNEASDDFNNLLNKEIESAKEVKLRKFANRNNNRNENSSKVKDASGFRLRVIQTDG
HKTKKTDPDYEVTIDGPLRKIEPYFFTYKTFCKERWRDRKLVDVFVSEFRDREPSYYSKT
IAEGKVYLNDEPANLDTIIRDGDLITHKVHRHEPPVTSKPIDIVFEDEDILVIDKPSSIP
VHPTGRYRFNTITKMLERQLGYSVHPCNRLDKPTSGLMFLAKTPLGADRMGDQMKAREVT
KEYVARVKGEFPIGIVEVDKPVRSVNPKVALNAVCEMSDENAKHAKTVFQRVSYDGQTSI
VKCKPLTGRTHQIRVHLQYLGFPIANDPIYSNPDIWGPDLGRGGLQNYDDIVLKLDAIGK
TNPAESWIHPHSEGEYLLGRQCEECEAEMYTDPGTNDLDLWLHAFRYESLERNSDTQKPL
WSYRTKYPEWALEPHRRYMEMAVKEAGKCGPTKTAFSVGAVLVHGTQVLATGYSRELPGN
THAEQCALIKYSQLHPNCPTIVPMGTVLYTTMEPCSFRLSGNEPCCDRILATQGAIGTVF
VGVMEPDTFVKNNTSLNKLESHGVNYIQIPGYEEECTIIAFKGHDNSDDKA |
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References |
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External Links | |
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General Reference | - Tzermia, M., Katsoulou, C., Alexandraki, D. (1997). "Sequence analysis of a 33.2 kb segment from the left arm of yeast chromosome XV reveals eight known genes and ten new open reading frames including homologues of ABC transporters, inositol phosphatases and human expressed sequence tags." Yeast 13:583-589.9178509
- Dujon, B., Albermann, K., Aldea, M., Alexandraki, D., Ansorge, W., Arino, J., Benes, V., Bohn, C., Bolotin-Fukuhara, M., Bordonne, R., Boyer, J., Camasses, A., Casamayor, A., Casas, C., Cheret, G., Cziepluch, C., Daignan-Fornier, B., Dang, D. V., de Haan, M., Delius, H., Durand, P., Fairhead, C., Feldmann, H., Gaillon, L., Kleine, K., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Nature 387:98-102.9169874
- Bacher, A., Baur, R., Oltmanns, O., Lingens, F. (1969). "Biosynthesis of riboflavin. Mutants accumulating 6-hydroxy-2,4,5-triaminopyrimidine." FEBS Lett 5:316-318.11947306
- Oltmanns, O., Bacher, A. (1972). "Biosynthesis of riboflavine in Saccharomyces cerevisiae: the role of genes rib 1 and rib 7 ." J Bacteriol 110:818-822.4555411
- Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
- Behm-Ansmant, I., Grosjean, H., Massenet, S., Motorin, Y., Branlant, C. (2004). "Pseudouridylation at position 32 of mitochondrial and cytoplasmic tRNAs requires two distinct enzymes in Saccharomyces cerevisiae." J Biol Chem 279:52998-53006.15466869
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