tRNA guanosine-2'-O-methyltransferase TRM3 (Q07527)

Identification
NametRNA guanosine-2'-O-methyltransferase TRM3
Synonyms
  • tRNA [Gm18] methyltransferase
  • tRNA methylase 3
Gene NameTRM3
Enzyme Class
Biological Properties
General FunctionInvolved in RNA binding
Specific FunctionSpecifically methylates guanosine-18 in various tRNAs
Cellular LocationCytoplasm
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00198S-AdenosylhomocysteineShow
YMDB00297S-AdenosylmethionineShow
GO Classification
Component
Not Available
Function
transferase activity
transferase activity, transferring one-carbon groups
methyltransferase activity
binding
nucleic acid binding
RNA binding
RNA methyltransferase activity
catalytic activity
Process
metabolic process
macromolecule metabolic process
cellular macromolecule metabolic process
RNA metabolic process
RNA processing
Gene Properties
Chromosome Locationchromosome 4
LocusYDL112W
Gene Sequence>4311 bp ATGGTTGGAGGTGCTCTCATCTGCAAATATTTACCACGCGAAGAGCAACTAAAGCTTATA TCAGACTTGATTCAGAATGACAGCTTAGAAGAGGTGCTGGAGTTGATTGAAACTTCGCCA CTGGATATAACGACGGACAGCAACATTGAAACACCAATCTTTGAAAAAATTACTGAGCAG GTCATTGCGTATGCTAGTATAGACGGTGAAGCCAGGGAAATGTTTCGCTCTTCGAGGGCT GAAATGAACAAGGCGCTAAGAACTTCTGCACAATTACTATGTTGTTTACCCAGTGTATGG CATAAATTTCAGGTGTGGATGAGCTACCGGTTAAATGACATCATTAGCGAAAACTATAAA CATCTCTTCAATGACAACTTTGGAAAAAAGATTGTACAACCATTTTTCGATTCTTTTGCT GAGGAACAAAACGCTAATATCAAGCATGAAAATCTGCACTTAGATATCCTTAGCCTGCTT CACTACTTAGAAGTTGTATATTTATTTGACGAATGTAAGAATGGTATATCGTCCAAATGC CTAGATTTTATCATTGTTCCATTGTTAGGCTGTAATTCGGAAGAGATTGCTGATTCCTGC TCCAAGTTAATGAGATGGCATATCAAATATTTATCGAAATGCTGCAATACCGATAGTAAC TTTGATAAGCTCATTTGGACATTTATCAAACAATTGTACGCTGAAGGCTCCCAACAAGCT TGGAAGCAAAAGAACAGTTTATCTTTTCTCCTAAGATTCCTTTTAGCAGCAGAGTTGTCT CCTGAACTTATTACTTATATAAAGACCGATGCTTATTGGAGGCACATTCAAACAGAGCTG GATAATGATGTGCACGAACACAGAAAATTGGCACTCTCAATTCTTAAATTAACAATTCAA AAACTGTCATCTCATGGTATAACATTGCAAACAACATTTTATAAATGCAATGATCTAGCA AATATCGAGATGCTGGGTAGCTGGAAGAAATTCACCACCTTATATGAAATGATTGCTCTG GACACATCATTGAATCAAATACAAGCGGCCAAACAAGATATAATAAAAATTTTTGATAAC GAGCATTTACACCATAGTTGGGGCCTTATCTTGCTTTCTACGGGATTGAAATCATCAATG GAGAGTGTCAGAAAATACATGATGACGTTAATGTTCTCGATTACAAATATGTCAGCATTC TCTTCAAACTTGCCATTATTGACAAAAACTCTTCTTCCAGCTGCAATGTCCGCTCATTAT TTTGATGTAAAAGGTGTCAGTTGTCCCCATGGTGAAAAACTTTCATTATTTGTCAATAAC TTACTTTCCCAAACAACAGAAGGCATATCAGATATATTATTTGAAATTTTGAAATTATTG GTGGAAAAAGGGACCTCTTTTGACCCTTCTAGGATATACTTGTCCTACGGTATCTTAGTT TTCTTCCAGAATAACAAACAGAAAACAATCAACTCTGACCATTTGAGTCTGATCAGGAAA TTGTACGATTTTGCAGCCGAAGAAGAGGTTCTCGAAACTACAATCCAAACAATTTACTTG AAGTTCTTACTGTACATTGATCCATCTGTCTCCGCATCTGAATTATTGTTTACATTAGTT TCACACATTAAGTTAAAGGGAGGGACATACAAATATGTTGAGCCTCTTTTTGAAGATTAT AGGGATCTTGCCGTTTCTCACTTTGACGATTTGCAAGCGAAAGAAAACTTGACGACAAAC ATCGGAAAGGATACTATTTTTGACCTCTTAGCATCTATCATCTTCGATTTCAAGGATATT GATATTACTCCCGATTTTTTAATTGAAGTTGCGAAGTCGAAGCAAGATATACCCGTTTAC ACTTCAAAGGCAGTTACTTTTCTAACTCAGCTACTTTCAGGCGAACCTTCGAACGGTTAT ACCTATGAAAATGCAACCGCTTTGTTGTCTTACCCAAATTTTACAATATCTACATGGAAA TCCATCAATGTGAATAATTTATTTAAGTCGGTAATGGAAAAATTTTCGCTTGATAAATTC AAGTTCTTTGCTGAAATTTACCAGAAGACTTATGAATGTAGGTTCGATACAATCGAGCTA AATTTTAATGACCTTCTGAGCCTATATGAAATGGTTAAAAAGTCTGCCAATCAGTGTTCA AGAGAGAGCTTTAAAGTGAAGGACAGCGCTTATTCTTCTTACTTTGAACTTTTAAATACC TTCCTGAAAACATACGCCTTAAATCGTGACTCTTCTGAAGGAAATGATGATGAGCTTCAC ATACTACTACGCTTAGTTGACGAGAATATTAACAAAGATAATGGGAATTATCTGGGGAAC CTGGCAGTCTGTAAATTATTGTACTTTATCATAGATTCCTACATCCATTGCTCCACATCT GTTTCTGATGATGATATTTTCATAGTGAAATTCATTTTTGAGAAATTTTCGTTTATATGG GAATGTATTAATAGTGAAAGATTAGTTTTGAAGGAAAGGGATCTGCATTTGATGCTAATT AAGGGATTATTTCATCCTGTGATCCTGTACTTTGGATCAAATCAGTATATCGATACTTTG ACCTCGAAGTTGGAGGAACATGCACAAACGATTATATCATTGAGTTATTCCAGAAGAAGC CTACTCCCTCTTCTCGGATCACAACTCAGAGTATTTATGAAGTTTTATGGGAAATTGTTA AGAGAAGATGTCAACTATTGGTGGTTGATTAACATTATAGTAGGTGTTTTCAAACAGCCG CAAATGGATGTCAACCTTTACAAGCTCAAACCTGTTATTTCCAGCCTATTTGACCACAAA CTGAACAATTATTACATAAAAGGCGATGAACTTTATGAAAAGGTGTACGGACCCGACGAA ATATTGGCTAGAGTATCTATTATTGATTCTATTTTATATGCAAATGATCAGTTGAAAATT CGATTAATCGAAAAAGTTACTGAAAAAACAAACGCACTTTATGCTATAAAGAGAACCGAC GGTGCCGAAGCGTTACAGAGGTTGTTGCAGTGGCAATTGTTATTATTATCACTACTAACC ACCAATGAAAAAAAATTAAGCGAAACTAGCATGATCAGAATTCTAAAGAGTATCGAGGAT GAAAGTTCACCACTAGTAAGAGTATATAAAGAATGGTTCATTTCCTCAAAGGTTGTTGAT TATTATAAAACTGGCAATCCAAAATTTGCTGAGGATTATCTCTTCTCATTGCTGGAGGAT CATAGCAAACCGGTGTTTGTAGTTAGCGCAGAAAAAATATGCTTTATGGTGTTGAAAGAT TTAAGAAACGACGAAAAGAAATATGGATTTACACAGTTACTAGACAGATTCATTTGCACG TTAGTTCCTAACGCAGCTTCAAACAAACCTCTGGTAAGGCATTTTTCTAATTCTCTAATA ATTTCATTATGGCCTACATTCAAAGCCTACCTATCGGATCACACACTGAGAAACATCATT GAGAACTTATATTCCAATGCCAAAAAGACTCAAATATTTGGTCAATATCGTGCCGGGGAT GCCAACATATGGGATCTGAAGGGTGATAGGAAATTAACGAACATGTTTGGTGGTGTGCTC AAAAAAGTTACCGACCATGATTGCCCGTACATTTCTGAGTCTGTTTTCGAAAAATATTTG CAGGAGAAAGATATTGTTCCTATAGGTACCGATGAAAGGTCCTTATGGCTTGATAAAAGA GACACCAATACGGAATCTGTTAACAACGCGAACATCTCCTGTGACACTTCTCCACTGCAA ACAAAAAGTGGAGCTTGGGAAACCGTTCTGGATCTGGACAATAAAAAATCGAATGATGTC GTTACACGTTCTGAATTGATTGTGGTATCTTCATTAGTTGATAAGCCGCCAAACCTGGGA GGTATTTGTAGGTTATGTGATGTTTTAGGTGTGGGACTGCTTACTGTACAAGACATCAAA GTCAAAAACCATCCTCAATTTAAAAATGTTGCTGTGACTGCTGATAGATGGATGCCCATG GAGGAAGTTGCCCTAGATGAGATTGCAAGTTTCATGAAAGAGAAAAAGAAGGAAGGGTAC ACATTAATTGGGCTAGAGCAAACGGATAAATCAGTGAAGCTAGACAACAATTTCCAATTC CCTAAAAAATCATTGATCTTGCTAGGAACCGAAGCATTCGGTATTCCGGGGACTTTGTTA AGCGAATTGGATTTATGTTTAGAGATTCAACAATTTGGTGTGATCAGATCAATGAATATT CAAACGGCAACCGCAGTTATCGTTCATTCCTATACAGTTCAACACATGTAA
Protein Properties
Pfam Domain Function
Protein Residues1436
Protein Molecular Weight165046.0
Protein Theoretical pI5.96
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>tRNA guanosine-2'-O-methyltransferase TRM3 MVGGALICKYLPREEQLKLISDLIQNDSLEEVLELIETSPLDITTDSNIETPIFEKITEQ VIAYASIDGEAREMFRSSRAEMNKALRTSAQLLCCLPSVWHKFQVWMSYRLNDIISENYK HLFNDNFGKKIVQPFFDSFAEEQNANIKHENLHLDILSLLHYLEVVYLFDECKNGISSKC LDFIIVPLLGCNSEEIADSCSKLMRWHIKYLSKCCNTDSNFDKLIWTFIKQLYAEGSQQA WKQKNSLSFLLRFLLAAELSPELITYIKTDAYWRHIQTELDNDVHEHRKLALSILKLTIQ KLSSHGITLQTTFYKCNDLANIEMLGSWKKFTTLYEMIALDTSLNQIQAAKQDIIKIFDN EHLHHSWGLILLSTGLKSSMESVRKYMMTLMFSITNMSAFSSNLPLLTKTLLPAAMSAHY FDVKGVSCPHGEKLSLFVNNLLSQTTEGISDILFEILKLLVEKGTSFDPSRIYLSYGILV FFQNNKQKTINSDHLSLIRKLYDFAAEEEVLETTIQTIYLKFLLYIDPSVSASELLFTLV SHIKLKGGTYKYVEPLFEDYRDLAVSHFDDLQAKENLTTNIGKDTIFDLLASIIFDFKDI DITPDFLIEVAKSKQDIPVYTSKAVTFLTQLLSGEPSNGYTYENATALLSYPNFTISTWK SINVNNLFKSVMEKFSLDKFKFFAEIYQKTYECRFDTIELNFNDLLSLYEMVKKSANQCS RESFKVKDSAYSSYFELLNTFLKTYALNRDSSEGNDDELHILLRLVDENINKDNGNYLGN LAVCKLLYFIIDSYIHCSTSVSDDDIFIVKFIFEKFSFIWECINSERLVLKERDLHLMLI KGLFHPVILYFGSNQYIDTLTSKLEEHAQTIISLSYSRRSLLPLLGSQLRVFMKFYGKLL REDVNYWWLINIIVGVFKQPQMDVNLYKLKPVISSLFDHKLNNYYIKGDELYEKVYGPDE ILARVSIIDSILYANDQLKIRLIEKVTEKTNALYAIKRTDGAEALQRLLQWQLLLLSLLT TNEKKLSETSMIRILKSIEDESSPLVRVYKEWFISSKVVDYYKTGNPKFAEDYLFSLLED HSKPVFVVSAEKICFMVLKDLRNDEKKYGFTQLLDRFICTLVPNAASNKPLVRHFSNSLI ISLWPTFKAYLSDHTLRNIIENLYSNAKKTQIFGQYRAGDANIWDLKGDRKLTNMFGGVL KKVTDHDCPYISESVFEKYLQEKDIVPIGTDERSLWLDKRDTNTESVNNANISCDTSPLQ TKSGAWETVLDLDNKKSNDVVTRSELIVVSSLVDKPPNLGGICRLCDVLGVGLLTVQDIK VKNHPQFKNVAVTADRWMPMEEVALDEIASFMKEKKKEGYTLIGLEQTDKSVKLDNNFQF PKKSLILLGTEAFGIPGTLLSELDLCLEIQQFGVIRSMNIQTATAVIVHSYTVQHM
References
External Links
ResourceLink
Saccharomyces Genome Database TRM3
Uniprot IDQ07527
Uniprot NameTRM3_YEAST
GenBank Gene IDZ74160
Genebank Protein ID1431162
General Reference
  • Jacq, C., Alt-Morbe, J., Andre, B., Arnold, W., Bahr, A., Ballesta, J. P., Bargues, M., Baron, L., Becker, A., Biteau, N., Blocker, H., Blugeon, C., Boskovic, J., Brandt, P., Bruckner, M., Buitrago, M. J., Coster, F., Delaveau, T., del Rey, F., Dujon, B., Eide, L. G., Garcia-Cantalejo, J. M., Goffeau, A., Gomez-Peris, A., Zaccaria, P., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Nature 387:75-78.9169867
  • Boskovic, J., Soler-Mira, A., Garcia-Cantalejo, J. M., Ballesta, J. P., Jimenez, A., Remacha, M. (1996). "The sequence of a 16,691 bp segment of Saccharomyces cerevisiae chromosome IV identifies the DUN1, PMT1, PMT5, SRP14 and DPR1 genes, and five new open reading frames." Yeast 12:1377-1384.8923743
  • Cavaille, J., Chetouani, F., Bachellerie, J. P. (1999). "The yeast Saccharomyces cerevisiae YDL112w ORF encodes the putative 2'-O-ribose methyltransferase catalyzing the formation of Gm18 in tRNAs." RNA 5:66-81.9917067
  • Huh, W. K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., O'Shea, E. K. (2003). "Global analysis of protein localization in budding yeast." Nature 425:686-691.14562095
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106