Aspartate aminotransferase, mitochondrial (Q01802)

Identification

Name

Aspartate aminotransferase, mitochondrial

Synonyms

Transaminase A

Gene Name

AAT1

Enzyme Class

2.6.1.1

Biological Properties

General Function

Involved in transferase activity, transferring nitrogenous groups

Specific Function

Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol

Cellular Location

Mitochondrion matrix

SMPDB Pathways

Asparagine metabolism	PW002274
Aspartate metabolism	PW002375
Phenylalanine metabolism	PW002437
Tyrosine metabolism	PW002441

KEGG Pathways

Alanine, aspartate and glutamate metabolism	ec00250
Arginine and proline metabolism	ec00330
Cysteine and methionine metabolism	ec00270
Phenylalanine metabolism	ec00360
Phenylalanine, tyrosine and tryptophan biosynthesis	ec00400

SMPDB Reactions

L-Glutamic acid + Oxalacetic acid → L-Aspartic acid + Oxoglutaric acid

L-Aspartic acid + Oxoglutaric acid → Oxalacetic acid + L-Glutamic acid

KEGG Reactions

L-Aspartic acid + Oxoglutaric acid ↔ L-Glutamic acid + Oxalacetic acid

Oxoglutaric acid + erythro-4-hydroxy-L-glutamic acid → 4-hydroxy-2-oxoglutaric acid + L-Glutamic acid

L-Glutamic acid + (4-hydroxyphenyl)pyruvic acid → L-Tyrosine + Oxoglutaric acid

Metabolites

YMDB ID	Name	View
YMDB00153	Oxoglutaric acid	Show
YMDB00235	Oxalacetic acid	Show
YMDB00271	L-Glutamic acid	Show
YMDB00364	L-Tyrosine	Show
YMDB00719	erythro-4-hydroxy-L-glutamic acid	Show
YMDB00722	4-hydroxy-2-oxoglutaric acid	Show
YMDB00896	L-Aspartic acid	Show
YMDB00958	(4-hydroxyphenyl)pyruvic acid	Show

GO Classification

Component
Not Available
Function
binding
transferase activity, transferring nitrogenous groups
transaminase activity
cofactor binding
pyridoxal phosphate binding
catalytic activity
transferase activity
Process
metabolic process
biosynthetic process
cellular metabolic process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process

Gene Properties

Chromosome Location

chromosome 11

Locus

YKL106W

Gene Sequence

>1356 bp ATGTTGAGGACGAGGCTTACTAATTGCAGTCTATGGAGGCCCTACTACACGTCATCGCTT AGTAGAGTACCAAGAGCACCTCCAGATAAAGTCTTGGGGTTATCTGAACACTTCAAAAAG GCAAAAAATGTTAACAAAATTGACCTGACCGTAGGAATATATAAAGATGGTTGGGGCAAA GTGACGACGTTTCCCTCCGTTGCAAAAGCTCAAAAATTGATTGAATCTCATTTAGAGTTG AATAAGAATCTTTCATATTTACCAATAACAGGTTCCAAAGAATTTCAGGAAAACGTTATG AAATTTTTGTTCAAGGAATCATGTCCGCAGTTTGGGCCATTTTATTTAGCCCATGATAGA ATCAGCTTTGTTCAGACTTTGAGTGGTACAGGCGCCCTAGCTGTAGCAGCTAAATTCTTG GCATTATTTATTTCAAGAGATATTTGGATACCTGACCCATCATGGGCAAATCATAAGAAC ATTTTTCAGAACAATGGTTTTGAAAATATTTACCGGTATTCCTATTATAAGGACGGTCAG ATAGACATCGACGGATGGATTGAGCAATTGAAAACCTTTGCATATAACAACCAGCAAGAA AACAATAAGAACCCACCTTGCATAATCTTGCATGCGTGTTGTCACAACCCTACAGGTCTC GACCCAACTAAAGAGCAATGGGAAAAGATTATAGATACTATATATGAGCTAAAAATGGTA CCCATTGTTGATATGGCTTATCAGGGTTTAGAGTCTGGTAACTTACTAAAGGACGCATAT TTATTGAGGCTATGTCTCAATGTAAATAAATATCCAAATTGGAGTAACGGTATCTTTCTT TGTCAATCTTTTGCCAAGAACATGGGCCTTTATGGTGAACGAGTTGGTTCCTTAAGCGTT ATCACGCCGGCAACTGCGAACAATGGAAAGTTCAACCCTCTACAACAGAAAAACTCATTG CAGCAAAATATTGACTCCCAATTAAAAAAGATTGTCAGAGGTATGTATTCTTCTCCACCA GGATACGGTTCTCGTGTGGTAAATGTAGTATTATCAGATTTCAAATTGAAACAGCAATGG TTCAAGGATGTTGATTTCATGGTTCAGAGATTGCATCACGTCAGACAGGAGATGTTTGAC CGTCTAGGGTGGCCGGATCTTGTAAATTTCGCACAACAGCACGGTATGTTTTACTATACA AGGTTTAGTCCAAAGCAAGTCGAAATATTGAGAAACAATTCATTCGTCTATTTAACAGGT GATGGTAGATTGTCGCTTAGCGGAGTCAATGATTCGAACGTTGATTACTTATGTGAATCT CTTGAAGCAGTCTCGAAAATGGACAAACTCGCATAA

Protein Properties

Pfam Domain Function

Aminotran_1_2 (PF00155 )

Protein Residues

451

Protein Molecular Weight

51795.10156

Protein Theoretical pI

9.22

Signalling Regions

None

Transmembrane Regions

None

Protein Sequence

>Aspartate aminotransferase, mitochondrial MLRTRLTNCSLWRPYYTSSLSRVPRAPPDKVLGLSEHFKKVKNVNKIDLTVGIYKDGWGK VTTFPSVAKAQKLIESHLELNKNLSYLPITGSKEFQENVMKFLFKESCPQFGPFYLAHDR ISFVQTLSGTGALAVAAKFLALFISRDIWIPDPSWANHKNIFQNNGFENIYRYSYYKDGQ IDIDGWIEQLKTFAYNNQQENNKNPPCIILHACCHNPTGLDPTKEQWEKIIDTIYELKMV PIVDMAYQGLESGNLLKDAYLLRLCLNVNKYPNWSNGIFLCQSFAKNMGLYGERVGSLSV ITPATANNGKFNPLQQKNSLQQNIDSQLKKIVRGMYSSPPGYGSRVVNVVLSDFKLKQQW FKDVDFMVQRLHHVRQEMFDRLGWPDLVNFAQQHGMFYYTRFSPKQVEILRNNYFVYLTG DGRLSLSGVNDSNVDYLCESLEAVSKMDKLA

References

External Links

Resource	Link
Saccharomyces Genome Database	AAT1
Uniprot ID	Q01802
Uniprot Name	AATM_YEAST
GenBank Gene ID	X68052
Genebank Protein ID	3318

General Reference

Morin, P. J., Subramanian, G. S., Gilmore, T. D. (1992). "AAT1, a gene encoding a mitochondrial aspartate aminotransferase in Saccharomyces cerevisiae." Biochim Biophys Acta 1171:211-214.1482685
Cheret, G., Pallier, C., Valens, M., Diagnan-Fornier, B., Fukuhara, H., Bolotin-Fukuhara, M., Sor, F. (1993). "The DNA sequence analysis of the HAP4-LAP4 region on chromosome XI of Saccharomyces cerevisiae suggests the presence of a second aspartate aminotransferase gene in yeast." Yeast 9:1259-1265.8109175
Dujon, B., Alexandraki, D., Andre, B., Ansorge, W., Baladron, V., Ballesta, J. P., Banrevi, A., Bolle, P. A., Bolotin-Fukuhara, M., Bossier, P., et, a. l. .. (1994). "Complete DNA sequence of yeast chromosome XI." Nature 369:371-378.8196765
Huh, W. K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., O'Shea, E. K. (2003). "Global analysis of protein localization in budding yeast." Nature 425:686-691.14562095
Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
Sickmann, A., Reinders, J., Wagner, Y., Joppich, C., Zahedi, R., Meyer, H. E., Schonfisch, B., Perschil, I., Chacinska, A., Guiard, B., Rehling, P., Pfanner, N., Meisinger, C. (2003). "The proteome of Saccharomyces cerevisiae mitochondria." Proc Natl Acad Sci U S A 100:13207-13212.14576278
Reinders, J., Zahedi, R. P., Pfanner, N., Meisinger, C., Sickmann, A. (2006). "Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics." J Proteome Res 5:1543-1554.16823961