Sterol O-acyltransferase 2 (P53629)

Identification
NameSterol O-acyltransferase 2
Synonyms
  • Sterol-ester synthase 2
Gene NameARE2
Enzyme Class
Biological Properties
General FunctionInvolved in O-acyltransferase activity
Specific FunctionEnsures probably most of the acyltransferase activity. Suppression of ARE2 reduces sterol ester levels to 25% of the normal value
Cellular LocationEndoplasmic reticulum membrane; Multi-pass membrane protein
SMPDB Pathways
Steroid biosynthesisPW002482 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
Steroid biosynthesisec00100 Map00100
SMPDB ReactionsNot Available
KEGG Reactions
ergosterol + oleoyl-CoA ↔ ergosteryl oleate + Coenzyme A
ergosterol ↔ ergosteryl palmitoleate + Coenzyme A
Metabolites
YMDB IDNameView
YMDB00045Coenzyme AShow
YMDB00313CholesterolShow
YMDB00539oleoyl-CoAShow
YMDB00542ergosteryl oleateShow
YMDB00543ergosterolShow
YMDB00551ergosteryl palmitoleateShow
GO ClassificationNot Available
Gene Properties
Chromosome Locationchromosome 14
LocusYNR019W
Gene Sequence>1929 bp ATGGACAAGAAGAAGGATCTACTGGAGAACGAACAATTTCTCCGCATCCAAAAGCTCAAC GCTGCCGATGCGGGCAAAAGACAATCTATAACAGTGGACGACGAGGGCGAACTATATGGG TTAGACACCTCCGGCAACTCACCAGCCAATGAACACACAGCTACCACAATTACACAGAAT CACAGCGTGGTGGCCTCAAACGGAGACGTCGCATTCATCCCAGGAACTGCTACCGAAGGC AATACAGAGATTGTAACTGAAGAAGTGATTGAGACCGATGATAACATGTTCAAGACCCAT GTGAAGACTTTAAGCTCCAAAGAGAAGGCACGGTATAGGCAAGGGTCCTCTAACTTTATA TCGTATTTCGATGATATGTCATTTGAACACAGGCCCAGTATATTAGATGGGTCAGTTAAC GAGCCCTTCAAGACCAAATTCGTGGGACCTACTTTAGAAAAGGAGATCAGAAGAAGGGAG AAAGAGCTAATGGCCATGCGCAAAAATTTACACCACCGCAAGTCCTCCCCAGATGCTGTC GACTCAGTAGGGAAAAATGATGGCGCCGCCCCAACTACTGTTCCAACTGCCGCCACCTCA GAAACGGTGGTCACCGTTGAAACCACCATAATTTCATCCAATTTCTCCGGGTTGTACGTG GCGTTTTGGATGGCTATTGCATTTGGTGCTGTCAAGGCTTTAATAGACTATTATTACCAG CATAATGGTAGCTTCAAGGATTCGGAGATCTTGAAATTTATGACTACGAATTTGTTCACT GTGGCATCCGTAGATCTTTTGATGTATTTGAGCACTTATTTTGTCGTTGGAATACAATAC TTATGCAAGTGGGGGGTCTTGAAATGGGGCACTACCGGCTGGATCTTCACCTCAATTTAC GAGTTTTTGTTTGTTATCTTCTACATGTATTTAACAGAAAACATCCTAAAACTACACTGG CTGTCCAAGATCTTCCTTTTTTTGCATTCTTTAGTTTTATTGATGAAAATGCATTCTTTC GCCTTCTACAATGGCTATCTATGGGGTATAAAGGAAGAACTACAATTTTCCAAAAGCGCT CTTGCCAAATACAAGGATTCTATAAATGATCCAAAAGTTATTGGTGCTCTTGAGAAAAGC TGTGAGTTTTGTAGTTTTGAATTGAGCTCTCAGTCTTTAAGCGACCAAACTCAAAAATTC CCCAACAATATCAGTGCAAAAAGCTTTTTTTGGTTCACCATGTTTCCAACCCTAATTTAC CAAATTGAATATCCAAGAACTAAGGAAATCAGATGGAGCTACGTATTAGAAAAGATCTGC GCCATCTTCGGTACCATTTTCTTAATGATGATAGATGCTCAAATCTTGATGTATCCTGTA GCAATGAGAGCATTGGCTGTGCGCAATTCTGAATGGACTGGTATATTGGATAGATTATTG AAATGGGTTGGATTGCTCGTTGATATCGTCCCAGGGTTTATCGTGATGTACATCTTGGAC TTCTATTTGATTTGGGATGCCATTTTGAACTGTGTGGCTGAATTGACAAGATTTGGCGAC AGATATTTCTACGGTGACTGGTGGAATTGTGTTAGTTGGGCAGACTTCAGTAGAATTTGG AACATCCCAGTGCATAAGTTTTTGTTAAGACATGTTTACCATAGTTCAATGAGTTCATTC AAATTGAACAAGAGTCAAGCAACTTTGATGACCTTTTTCTTAAGTTCCGTCGTTCATGAA TTAGCAATGTACGTTATCTTCAAGAAATTGAGGTTTTACTTGTTCTTCTTCCAAATGCTG CAAATGCCATTAGTAGCTTTAACAAATACTAAATTCATGAGGAACAGAACCATAATCGGA AATGTTATTTTCTGGCTCGGTATCTGCATGGGACCAAGTGTCATGTGTACGTTGTACTTG ACATTCTAA
Protein Properties
Pfam Domain Function
Protein Residues642
Protein Molecular Weight74022.20313
Protein Theoretical pI7.8
Signalling Regions
  • None
Transmembrane Regions
  • 215-235
  • 292-312
  • 404-424
  • 442-462
  • 485-505
  • 567-587
  • 622-642
Protein Sequence>Sterol O-acyltransferase 2 MDKKKDLLENEQFLRIQKLNAADAGKRQSITVDDEGELYGLDTSGNSPANEHTATTITQN HSVVASNGDVAFIPGTATEGNTEIVTEEVIETDDNMFKTHVKTLSSKEKARYRQGSSNFI SYFDDMSFEHRPSILDGSVNEPFKTKFVGPTLEKEIRRREKELMAMRKNLHHRKSSPDAV DSVGKNDGAAPTTVPTAATSETVVTVETTIISSNFSGLYVAFWMAIAFGAVKALIDYYYQ HNGSFKDSEILKFMTTNLFTVASVDLLMYLSTYFVVGIQYLCKWGVLKWGTTGWIFTSIY EFLFVIFYMYLTENILKLHWLSKIFLFLHSLVLLMKMHSFAFYNGYLWGIKEELQFSKSA LAKYKDSINDPKVIGALEKSCEFCSFELSSQSLSDQTQKFPNNISAKSFFWFTMFPTLIY QIEYPRTKEIRWSYVLEKICAIFGTIFLMMIDAQILMYPVAMRALAVRNSEWTGILDRLL KWVGLLVDIVPGFIVMYILDFYLIWDAILNCVAELTRFGDRYFYGDWWNCVSWADFSRIW NIPVHKFLLRHVYHSSMSSFKLNKSQATLMTFFLSSVVHELAMYVIFKKLRFYLFFFQML QMPLVALTNTKFMRNRTIIGNVIFWLGICMGPSVMCTLYLTF
References
External Links
ResourceLink
Saccharomyces Genome Database ARE2
Uniprot IDP53629
Uniprot NameARE2_YEAST
GenBank Gene IDU51790
Genebank Protein ID1373171
General Reference
  • Yang, H., Bard, M., Bruner, D. A., Gleeson, A., Deckelbaum, R. J., Aljinovic, G., Pohl, T. M., Rothstein, R., Sturley, S. L. (1996). "Sterol esterification in yeast: a two-gene process." Science 272:1353-1356.8650549
  • Yu, C., Kennedy, N. J., Chang, C. C., Rothblatt, J. A. (1996). "Molecular cloning and characterization of two isoforms of Saccharomyces cerevisiae acyl-CoA:sterol acyltransferase." J Biol Chem 271:24157-24163.8798656
  • Philippsen, P., Kleine, K., Pohlmann, R., Dusterhoft, A., Hamberg, K., Hegemann, J. H., Obermaier, B., Urrestarazu, L. A., Aert, R., Albermann, K., Altmann, R., Andre, B., Baladron, V., Ballesta, J. P., Becam, A. M., Beinhauer, J., Boskovic, J., Buitrago, M. J., Bussereau, F., Coster, F., Crouzet, M., D'Angelo, M., Dal Pero, F., De Antoni, A., Del Rey, F., Doignon, F., Domdey, H., Dubois, E., Fiedler, T., Fleig, U., Floeth, M., Fritz, C., Gaillardin, C., Garcia-Cantalejo, J. M., Glansdorff, N. N., Goffeau, A., Gueldener, U., Herbert, C., Heumann, K., Heuss-Neitzel, D., Hilbert, H., Hinni, K., Iraqui Houssaini, I., Jacquet, M., Jimenez, A., Jonniaux, J. L., Karpfinger, L., Lanfranchi, G., Lepingle, A., Levesque, H., Lyck, R., Maftahi, M., Mallet, L., Maurer, K. C., Messenguy, F., Mewes, H. W., Mosti, D., Nasr, F., Nicaud, J. M., Niedenthal, R. K., Pandolfo, D., Pierard, A., Piravandi, E., Planta, R. J., Pohl, T. M., Purnelle, B., Rebischung, C., Remacha, M., Revuelta, J. L., Rinke, M., Saiz, J. E., Sartorello, F., Scherens, B., Sen-Gupta, M., Soler-Mira, A., Urbanus, J. H., Valle, G., Van Dyck, L., Verhasselt, P., Vierendeels, F., Vissers, S., Voet, M., Volckaert, G., Wach, A., Wambutt, R., Wedler, H., Zollner, A., Hani, J. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Nature 387:93-98.9169873
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Li, X., Gerber, S. A., Rudner, A. D., Beausoleil, S. A., Haas, W., Villen, J., Elias, J. E., Gygi, S. P. (2007). "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." J Proteome Res 6:1190-1197.17330950
  • Chi, A., Huttenhower, C., Geer, L. Y., Coon, J. J., Syka, J. E., Bai, D. L., Shabanowitz, J., Burke, D. J., Troyanskaya, O. G., Hunt, D. F. (2007). "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Proc Natl Acad Sci U S A 104:2193-2198.17287358
  • Smolka, M. B., Albuquerque, C. P., Chen, S. H., Zhou, H. (2007). "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Proc Natl Acad Sci U S A 104:10364-10369.17563356
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956