Aromatic amino acid aminotransferase 2 (P38840)

Identification

Name

Aromatic amino acid aminotransferase 2

Synonyms

Aromatic amino acid aminotransferase II
Aromatic amino acid-requiring protein 9

Gene Name

ARO9

Enzyme Class

2.6.1.57

Biological Properties

General Function

Involved in transferase activity, transferring nitrogenous groups

Specific Function

Has aromatic amino acid transaminase activity and kynurenine aminotransferase activity

Cellular Location

Cytoplasm

SMPDB Pathways

Methionine metabolism and salvage	PW002384
Phenylalanine metabolism	PW002437
Tryptophan metabolism	PW002442
Tyrosine metabolism	PW002441

KEGG Pathways

Tyrosine metabolism

ec00350

SMPDB Reactions

4-Methylthio-2-oxobutanoate + L-Phenylalanine → L-Methionine + 2-Ketobutyric acid

keto-phenylpyruvic acid + L-Alanine ↔ Pyruvic acid + L-Phenylalanine

(4-hydroxyphenyl)pyruvic acid + L-Alanine ↔ Pyruvic acid + L-Tyrosine

L-Tryptophan + keto-phenylpyruvic acid → 3-(indol-3-yl)pyruvate + L-Phenylalanine

KEGG Reactions

Oxoglutaric acid + L-Phenylalanine ↔ L-Glutamic acid + keto-phenylpyruvic acid

Oxoglutaric acid + L-Tryptophan ↔ L-Glutamic acid + 3-(indol-3-yl)pyruvic acid

L-Glutamic acid + (4-hydroxyphenyl)pyruvic acid → L-Tyrosine + Oxoglutaric acid

Metabolites

YMDB ID	Name	View
YMDB00071	2-Ketobutyric acid	Show
YMDB00126	L-Tryptophan	Show
YMDB00153	Oxoglutaric acid	Show
YMDB00154	L-Alanine	Show
YMDB00169	3-(indol-3-yl)pyruvic acid	Show
YMDB00175	Pyruvic acid	Show
YMDB00271	L-Glutamic acid	Show
YMDB00304	L-Phenylalanine	Show
YMDB00318	L-Methionine	Show
YMDB00364	L-Tyrosine	Show
YMDB00421	4-Methylthio-2-oxobutanoate	Show
YMDB00786	keto-phenylpyruvic acid	Show
YMDB00958	(4-hydroxyphenyl)pyruvic acid	Show
YMDB01069	3-(indol-3-yl)pyruvate	Show

GO Classification

Component
Not Available
Function
catalytic activity
transferase activity
binding
transferase activity, transferring nitrogenous groups
cofactor binding
pyridoxal phosphate binding
Process
metabolic process
biosynthetic process

Gene Properties

Chromosome Location

chromosome 8

Locus

YHR137W

Gene Sequence

>1542 bp ATGACTGCTGGTTCTGCCCCCCCTGTTGATTACACTTCCTTAAAGAAGAACTTCCAACCG TTTCTCTCCAGAAGAGTAGAAAATAGATCTCTGAAAAGCTTTTGGGATGCTTCTGATATC TCAGATGACGTCATTGAGCTAGCTGGTGGAATGCCAAACGAGAGATTTTTTCCTATCGAA TCTATGGATTTGAAAATATCAAAAGTTCCTTTTAATGATAACCCAAAATGGCATAATTCG TTTACCACGGCGCATTTGGACTTGGGATCCCCCAGTGAGCTACCCATTGCACGTTCTTTC CAATATGCAGAAACCAAGGGTTTACCCCCTCTCTTACATTTTGTTAAAGATTTTGTGTCC AGAATTAATCGCCCAGCCTTTTCCGATGAGACGGAGTCTAACTGGGATGTCATCCTTTCT GGCGGGTCCAACGATTCAATGTTTAAGGTTTTTGAAACAATTTGCGACGAATCGACCACT GTGATGATTGAAGAGTTTACTTTCACCCCGGCTATGTCCAATGTGGAGGCTACAGGAGCA AAAGTCATCCCCATCAAGATGAACCTGACCTTCGACAGAGAGTCCCAGGGTATTGATGTC GAATATCTAACGCAGTTGCTCGATAATTGGTCAACTGGACCATACAAAGACTTAAACAAG CCAAGGGTCCTATATACCATTGCAACGGGCCAAAATCCTACCGGGATGTCTGTCCCCCAG TGGAAAAGAGAGAAAATTTACCAGTTGGCCCAAAGACACGATTTCCTCATTGTTGAAGAT GATCCCTACGGTTATCTGTACTTTCCTTCCTATAATCCGCAAGAGCCATTAGAAAACCCT TACCATTCTAGCGACCTGACTACTGAACGGTATTTGAATGATTTTTTAATGAAATCATTC TTGACTTTGGATACAGATGCCCGTGTCATCCGTTTGGAGACTTTTTCTAAAATTTTTGCT CCTGGATTAAGGTTATCCTTCATCGTTGCTAATAAATTCCTTTTGCAAAAAATCTTGGAT TTGGCCGACATTACTACAAGGGCCCCCAGTGGTACCTCACAAGCTATTGTTTATTCTACA ATAAAGGCAATGGCTGAGTCCAACTTATCGTCCTCTCTTTCTATGAAAGAAGCAATGTTT GAGGGTTGGATAAGATGGATAATGCAGATTGCTTCTAAATACAATCATAGGAAAAATCTT ACTTTGAAAGCCTTATACGAAACAGAATCTTACCAAGCTGGTCAGTTTACCGTTATGGAA CCCTCCGCGGGTATGTTCATCATTATTAAAATCAATTGGGGGAATTTCGATAGACCTGAC GATTTGCCGCAACAGATGGATATTTTAGATAAGTTCTTGCTGAAGAATGGTGTTAAAGTA GTGCTTGGTTATAAAATGGCTGTTTGCCCAAATTATTCAAAGCAGAATTCAGATTTTCTA AGACTCACCATCGCCTATGCAAGGGATGATGATCAGTTGATTGAAGCTTCCAAAAGAATC GGTAGTGGCATAAAAGAATTTTTTGACAACTATAAAAGTTGA

Protein Properties

Pfam Domain Function

Aminotran_1_2 (PF00155 )

Protein Residues

513

Protein Molecular Weight

58527.0

Protein Theoretical pI

5.16

Signalling Regions

None

Transmembrane Regions

None

Protein Sequence

>Aromatic amino acid aminotransferase 2 MTAGSAPPVDYTSLKKNFQPFLSRRVENRSLKSFWDASDISDDVIELAGGMPNERFFPIE SMDLKISKVPFNDNPKWHNSFTTAHLDLGSPSELPIARSFQYAETKGLPPLLHFVKDFVS RINRPAFSDETESNWDVILSGGSNDSMFKVFETICDESTTVMIEEFTFTPAMSNVEATGA KVIPIKMNLTFDRESQGIDVEYLTQLLDNWSTGPYKDLNKPRVLYTIATGQNPTGMSVPQ WKREKIYQLAQRHDFLIVEDDPYGYLYFPSYNPQEPLENPYHSSDLTTERYLNDFLMKSF LTLDTDARVIRLETFSKIFAPGLRLSFIVANKFLLQKILDLADITTRAPSGTSQAIVYST IKAMAESNLSSSLSMKEAMFEGWIRWIMQIASKYNHRKNLTLKALYETESYQAGQFTVME PSAGMFIIIKINWGNFDRPDDLPQQMDILDKFLLKNGVKVVLGYKMAVCPNYSKQNSDFL RLTIAYARDDDQLIEASKRIGSGIKEFFDNYKS

References

External Links

Resource	Link
Saccharomyces Genome Database	ARO9
Uniprot ID	P38840
Uniprot Name	ARO9_YEAST
GenBank Gene ID	U10398
Genebank Protein ID	500670

General Reference

Iraqui, I., Vissers, S., Cartiaux, M., Urrestarazu, A. (1998). "Characterisation of Saccharomyces cerevisiae ARO8 and ARO9 genes encoding aromatic aminotransferases I and II reveals a new aminotransferase subfamily." Mol Gen Genet 257:238-248.9491083
Johnston, M., Andrews, S., Brinkman, R., Cooper, J., Ding, H., Dover, J., Du, Z., Favello, A., Fulton, L., Gattung, S., et, a. l. .. (1994). "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Science 265:2077-2082.8091229
Urrestarazu, A., Vissers, S., Iraqui, I., Grenson, M. (1998). "Phenylalanine- and tyrosine-auxotrophic mutants of Saccharomyces cerevisiae impaired in transamination." Mol Gen Genet 257:230-237.9491082
Iraqui, I., Vissers, S., Andre, B., Urrestarazu, A. (1999). "Transcriptional induction by aromatic amino acids in Saccharomyces cerevisiae." Mol Cell Biol 19:3360-3371.10207060
Huh, W. K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., O'Shea, E. K. (2003). "Global analysis of protein localization in budding yeast." Nature 425:686-691.14562095
Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956