Pyridoxamine 5'-phosphate oxidase (P38075)

Identification
NamePyridoxamine 5'-phosphate oxidase
Synonyms
  • PNP/PMP oxidase
  • PNPOx
Gene NamePDX3
Enzyme Class
Biological Properties
General FunctionInvolved in pyridoxamine-phosphate oxidase activity
Specific FunctionCatalyzes the oxidation of either pyridoxine 5'- phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP)
Cellular LocationNot Available
SMPDB PathwaysNot Available
KEGG Pathways
Vitamin B6 metabolismec00750 Map00750
SMPDB ReactionsNot Available
KEGG Reactions
water + oxygen + Ammonium + Pyridoxal ↔ Hydrogen peroxide + Pyridoxamine
water + Pyridoxamine 5'-phosphate + oxygen → Pyridoxal 5'-phosphate + Hydrogen peroxide + Ammonium
Pyridoxine 5'-phosphate + oxygen ↔ Pyridoxal 5'-phosphate + Hydrogen peroxide
Pyridoxine + oxygen ↔ Hydrogen peroxide + Pyridoxal
Metabolites
YMDB IDNameView
YMDB00037PyridoxineShow
YMDB00085Flavin MononucleotideShow
YMDB00091AmmoniaShow
YMDB00363Pyridoxal 5'-phosphateShow
YMDB00392PyridoxalShow
YMDB00397Pyridoxamine 5'-phosphateShow
YMDB00407Pyridoxine 5'-phosphateShow
YMDB00423AmmoniumShow
YMDB00686FMNH2Show
YMDB00888Hydrogen peroxideShow
YMDB00889PyridoxamineShow
YMDB00890waterShow
YMDB00900oxygenShow
GO Classification
Component
Not Available
Function
FMN binding
oxidoreductase activity, acting on the CH-NH2 group of donors
oxidoreductase activity
oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor
pyridoxamine-phosphate oxidase activity
catalytic activity
binding
nucleotide binding
Process
oxidation reduction
small molecule metabolic process
vitamin metabolic process
water-soluble vitamin metabolic process
vitamin B6 metabolic process
pyridoxine metabolic process
pyridoxine biosynthetic process
metabolic process
Gene Properties
Chromosome Locationchromosome 2
LocusYBR035C
Gene Sequence>687 bp ATGACTAAACAAGCTGAGGAGACCCAAAAGCCAATCATATTTGCTCCTGAGACGTATCAA TATGATAAATTTACTTTGAATGAAAAACAACTTACTGACGACCCAATCGATCTTTTCACC AAATGGTTCAACGAAGCCAAGGAAGACCCAAGGGAAACGTTGCCAGAAGCAATTACTTTT TCATCCGCGGAACTACCTAGTGGGAGGGTGTCGTCCAGGATTCTTCTTTTTAAGGAGCTC GACCATAGAGGTTTTACTATTTATTCTAACTGGGGAACCTCTAGAAAGGCTCATGATATT GCTACCAACCCGAATGCGGCAATCGTATTCTTTTGGAAGGATCTGCAAAGGCAGGTGAGA GTTGAAGGTATCACAGAGCATGTTAACAGAGAAACTTCTGAAAGATACTTTAAGACGAGA CCTCGTGGATCCAAGATCGGTGCATGGGCTTCCCGCCAATCGGATGTTATCAAGAACAGA GAAGAACTAGACGAGTTGACCCAAAAAAACACCGAACGTTTCAAGGATGCTGAAGACATC CCATGTCCAGATTATTGGGGTGGCTTGAGAATCGTTCCACTGGAAATTGAGTTCTGGCAA GGTAGACCCTCGAGATTGCATGATAGATTCGTTTACAGAAGAAAAACAGAAAACGATCCA TGGAAAGTCGTTAGACTAGCCCCATGA
Protein Properties
Pfam Domain Function
Protein Residues228
Protein Molecular Weight26908.0
Protein Theoretical pI7.67
PDB Fileshow
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Pyridoxamine 5'-phosphate oxidase MTKQAEETQKPIIFAPETYQYDKFTLNEKQLTDDPIDLFTKWFNEAKEDPRETLPEAITF SSAELPSGRVSSRILLFKELDHRGFTIYSNWGTSRKAHDIATNPNAAIVFFWKDLQRQVR VEGITEHVNRETSERYFKTRPRGSKIGAWASRQSDVIKNREELDELTQKNTERFKDAEDI PCPDYWGGLRIVPLEIEFWQGRPSRLHDRFVYRRKTENDPWKVVRLAP
References
External Links
ResourceLink
Saccharomyces Genome Database PDX3
Uniprot IDP38075
Uniprot NamePDX3_YEAST
GenBank Gene IDAY557712
Genebank Protein ID45269315
PDB ID
1CI0
General Reference
  • Loubbardi, A., Marcireau, C., Karst, F., Guilloton, M. (1995). "Sterol uptake induced by an impairment of pyridoxal phosphate synthesis in Saccharomyces cerevisiae: cloning and sequencing of the PDX3 gene encoding pyridoxine (pyridoxamine) phosphate oxidase." J Bacteriol 177:1817-1823.7896706
  • Smits, P. H., De Haan, M., Maat, C., Grivell, L. A. (1994). "The complete sequence of a 33 kb fragment on the right arm of chromosome II from Saccharomyces cerevisiae reveals 16 open reading frames, including ten new open reading frames, five previously identified genes and a homologue of the SCO1 gene." Yeast 10 Suppl A:S75-S80.8091864
  • Feldmann, H., Aigle, M., Aljinovic, G., Andre, B., Baclet, M. C., Barthe, C., Baur, A., Becam, A. M., Biteau, N., Boles, E., Brandt, T., Brendel, M., Bruckner, M., Bussereau, F., Christiansen, C., Contreras, R., Crouzet, M., Cziepluch, C., Demolis, N., Delaveau, T., Doignon, F., Domdey, H., Dusterhus, S., Dubois, E., Dujon, B., El Bakkoury, M., Entian, K. D., Feurmann, M., Fiers, W., Fobo, G. M., Fritz, C., Gassenhuber, H., Glandsdorff, N., Goffeau, A., Grivell, L. A., de Haan, M., Hein, C., Herbert, C. J., Hollenberg, C. P., Holmstrom, K., Jacq, C., Jacquet, M., Jauniaux, J. C., Jonniaux, J. L., Kallesoe, T., Kiesau, P., Kirchrath, L., Kotter, P., Korol, S., Liebl, S., Logghe, M., Lohan, A. J., Louis, E. J., Li, Z. Y., Maat, M. J., Mallet, L., Mannhaupt, G., Messenguy, F., Miosga, T., Molemans, F., Muller, S., Nasr, F., Obermaier, B., Perea, J., Pierard, A., Piravandi, E., Pohl, F. M., Pohl, T. M., Potier, S., Proft, M., Purnelle, B., Ramezani Rad, M., Rieger, M., Rose, M., Schaaff-Gerstenschlager, I., Scherens, B., Schwarzlose, C., Skala, J., Slonimski, P. P., Smits, P. H., Souciet, J. L., Steensma, H. Y., Stucka, R., Urrestarazu, A., van der Aart, Q. J., van Dyck, L., Vassarotti, A., Vetter, I., Vierendeels, F., Vissers, S., Wagner, G., de Wergifosse, P., Wolfe, K. H., Zagulski, M., Zimmermann, F. K., Mewes, H. W., Kleine, K. (1994). "Complete DNA sequence of yeast chromosome II." EMBO J 13:5795-5809.7813418
  • Hu, Y., Rolfs, A., Bhullar, B., Murthy, T. V., Zhu, C., Berger, M. F., Camargo, A. A., Kelley, F., McCarron, S., Jepson, D., Richardson, A., Raphael, J., Moreira, D., Taycher, E., Zuo, D., Mohr, S., Kane, M. F., Williamson, J., Simpson, A., Bulyk, M. L., Harlow, E., Marsischky, G., Kolodner, R. D., LaBaer, J. (2007). "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Genome Res 17:536-543.17322287
  • Peng, J., Schwartz, D., Elias, J. E., Thoreen, C. C., Cheng, D., Marsischky, G., Roelofs, J., Finley, D., Gygi, S. P. (2003). "A proteomics approach to understanding protein ubiquitination." Nat Biotechnol 21:921-926.12872131