| Identification |
|---|
| Name | Polyamine transporter TPO5 |
|---|
| Synonyms | Not Available |
|---|
| Gene Name | TPO5 |
|---|
| Enzyme Class | Not Available |
|---|
| Biological Properties |
|---|
| General Function | Involved in transport |
|---|
| Specific Function | Required for polyamine transport. Transports putrescine effectively and spermidine less effectively |
|---|
| Cellular Location | Golgi apparatus membrane; Multi-pass membrane protein |
|---|
| SMPDB Pathways | Not Available |
|---|
| KEGG Pathways | Not Available |
|---|
| SMPDB Reactions | Not Available |
|---|
| KEGG Reactions | Not Available |
|---|
| Metabolites | | YMDB ID | Name | View |
|---|
| YMDB00132 | Putrescine | Show | | YMDB00232 | Spermidine | Show |
|
|---|
| GO Classification | | Component |
|---|
| cell part | | membrane | | membrane part | | intrinsic to membrane | | integral to membrane | | Function |
|---|
| amine transmembrane transporter activity | | amino acid transmembrane transporter activity | | transporter activity | | transmembrane transporter activity | | active transmembrane transporter activity | | Process |
|---|
| amine transport | | amino acid transport | | transmembrane transport | | establishment of localization | | transport |
|
|---|
| Gene Properties |
|---|
| Chromosome Location | chromosome 11 |
|---|
| Locus | YKL174C |
|---|
| Gene Sequence | >1857 bp
ATGCCAGAGTATACGCTACTGGCTGATAATATAAGGGAGAATATCGTTCATTTCGATCCG
AATGGTTTGTTTGATAACTTGCACACCATTGTTCATGAAGATGACAGTCAAGAGAACGAG
GAGGCCGAGCATTTCAATTATGATCAGGTGTTGGATAAATCGTTATTGTCAAGAGGTTCT
ATTGTCGGTCTCGGTTTAGGACTAATGAGTCCCGTTTTAGGAATGTGCACTAGTATGGCC
ATTGGGCTAATTAATGGTGGTCCGTTAACTATAATGCTAGGTTTTTTAATCAGTGGAGTG
TGTATATGGTTTTCGTCGCTTTCTCTTGGTGAGATTGTTTCAAAATTTCCGATGGAACTG
CATGTTGGGAGTGCCATGTTGGCCCCGGAGAAATTGAAATTAGTATGTTCGTGGTACACT
GGCTGGTTAATGCTCATAGGGAATTGGACTATGAGTACCAGTATTACTTTTGCAGGCGCT
CAACTTACCATTTCTTTGATTCTGATGACGAACTCCAACCTAATATCCGAGGCACACTTG
ATTTTTTACACAGTCATTGTATTTTACTTAGTTGTGACTGTTGTAGGCCTCGTGAATTTG
AAATTTGCAAGATTTATTGAAACAATAAACAAAGTCTGTGTTTATTGGATCATATATGCC
ATTATATTTATTGATATTCTTCTACTAGTATTCCACAAAGGTAAATTTCGATCTTTGAAG
TACGCGCTATTTCACTTTGATAATAATCTATCAGGGTATAAAAGCGCATTTCTTTCCTTC
ATCATTGGATTCCAACAGTCTAATTTCACGTTACAAGGTTTCAGTATGTTACCTGCTTTA
GCTGACGAAGTCAAAGTTCCTGAGAAGGATATTCCACGTGGTATGTCGAATGCGGTATTG
TTATCCGCGTTCTCTGGAGTCATTTTTCTTATACCAATAATGTTAATCCTGCCAGATAAT
GATTTGCTTTTTACCAATCATAAGGTTCTACCAATAGTGAACATTTTTACAAAATCGACT
GATTCGGTGGTCTTGTCTTTTTTTTTAGTGCTCCTAATTTTAGGAAACTTACTGTTTTCC
GGAATTGGCTCGATTACTACATCTTCTCGTGCGGTATATAGTTTTAGTCGTGACCAGGCT
ATACCATACTACGATAAATGGACCTACGTCGAACCGGATTCTCAGTCAAAAGTCCCCAAG
AATTCTGTTGTATTGAGTATGATAATATCATACTTTTTAGGTCTGCTAGCTTTGATTTCA
ACGGCCGCATTTAATGCTTTTATAGGCGCTGCAGTGCTCTGTCTTTGTTCTGCGACTTTC
ATTCCGTTAGTCTTGGTGCTGTTTACGAGAAGAAGAGCTATCCGAAGCGCGCCAGTAAAA
ATCAGGTATAAGTTTGGTTGGTTCATCAACATTGTTTCTATTGTGTGGCTCTTGTTATCT
ATGGTTTCTGTTTGCCTACCAACGCAAGTGCCTGTAACTTTCAAAACAATGAATTATGCT
TTAATGGTGTACGTATTCTGCATTTTAGTTATCACTGGTCTTTATTTCAAATGGGGGAAG
TATAATTTTAGATTACCCTTGGCAGATGACATCAAGGCTCCAATTCCCAGTGATGCGGAA
GAAACTGTTTTTGAACTAGAGGATAGCAATGTTGAACATACTCTAAACTCGGGAACCACA
GTGAAAGAGTCTGTAGAAAATAATTCTGAAGAAGGTTTCATCAAGGTGCATCCTAAAAGT
AGTACAGAAAATCCCTTTGAGGAAAATGAGGAAAACGTGATAACCGATTATGGTGATGAG
CACCATACAGCAGAACAAGAATTTGATCTTGCCGATGATCGTAGATATGATATATGA |
|---|
| Protein Properties |
|---|
| Pfam Domain Function | |
|---|
| Protein Residues | 618 |
|---|
| Protein Molecular Weight | 69242.0 |
|---|
| Protein Theoretical pI | 5.02 |
|---|
| Signalling Regions | |
|---|
| Transmembrane Regions | - 61-84
- 91-110
- 132-148
- 155-171
- 180-200
- 212-231
- 298-317
- 343-367
- 403-419
- 426-449
- 465-486
- 499-516
|
|---|
| Protein Sequence | >Polyamine transporter TPO5
MPEYTLLADNIRENIVHFDPNGLFDNLHTIVHEDDSQENEEAEHFNYDQVLDKSLLSRGS
IVGLGLGLMSPVLGMCTSMAIGLINGGPLTIMLGFLISGVCIWFSSLSLGEIVSKFPMEL
HVGSAMLAPEKLKLVCSWYTGWLMLIGNWTMSTSITFAGAQLTISLILMTNSNLISEAHL
IFYTVIVFYLVVTVVGLVNLKFARFIETINKVCVYWIIYAIIFIDILLLVFHKGKFRSLK
YALFHFDNNLSGYKSAFLSFIIGFQQSNFTLQGFSMLPALADEVKVPEKDIPRGMSNAVL
LSAFSGVIFLIPIMLILPDNDLLFTNHKVLPIVNIFTKSTDSVVLSFFLVLLILGNLLFS
GIGSITTSSRAVYSFSRDQAIPYYDKWTYVEPDSQSKVPKNSVVLSMIISYFLGLLALIS
TAAFNAFIGAAVLCLCSATFIPLVLVLFTRRRAIRSAPVKIRYKFGWFINIVSIVWLLLS
MVSVCLPTQVPVTFKTMNYALMVYVFCILVITGLYFKWGKYNFRLPLADDIKAPIPSDAE
ETVFELEDSNVEHTLNSGTTVKESVENNSEEGFIKVHPKSSTENPFEENEENVITDYGDE
HHTAEQEFDLADDRRYDI |
|---|
| References |
|---|
| External Links | |
|---|
| General Reference | - Vandenbol, M., Bolle, P. A., Dion, C., Portetelle, D., Hilger, F. (1994). "Sequencing and analysis of a 20.5 kb DNA segment located on the left arm of yeast chromosome XI." Yeast 10 Suppl A:S25-S33.8091858
- Dujon, B., Alexandraki, D., Andre, B., Ansorge, W., Baladron, V., Ballesta, J. P., Banrevi, A., Bolle, P. A., Bolotin-Fukuhara, M., Bossier, P., et, a. l. .. (1994). "Complete DNA sequence of yeast chromosome XI." Nature 369:371-378.8196765
- Peng, J., Schwartz, D., Elias, J. E., Thoreen, C. C., Cheng, D., Marsischky, G., Roelofs, J., Finley, D., Gygi, S. P. (2003). "A proteomics approach to understanding protein ubiquitination." Nat Biotechnol 21:921-926.12872131
- Tachihara, K., Uemura, T., Kashiwagi, K., Igarashi, K. (2005). "Excretion of putrescine and spermidine by the protein encoded by YKL174c (TPO5) in Saccharomyces cerevisiae." J Biol Chem 280:12637-12642.15668236
- Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258
- Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956
|
|---|