Identification |
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Name | Squalene synthase |
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Synonyms | - SQS
- SS
- FPP:FPP farnesyltransferase
- Farnesyl-diphosphate farnesyltransferase
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Gene Name | ERG9 |
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Enzyme Class | |
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Biological Properties |
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General Function | Involved in transferase activity |
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Specific Function | May regulate the flux of isoprene intermediates through the sterol pathway. Squalene synthase is crucial for balancing the incorporation of farnesyl diphosphate (FPP) into sterol and nonsterol isoprene synthesis. ERG9 is also essential for cell growth in yeast |
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Cellular Location | Endoplasmic reticulum membrane; Multi-pass membrane protein |
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SMPDB Pathways | Cholesterol biosynthesis and metabolism CE(10:0) | PW002545 | | Cholesterol biosynthesis and metabolism CE(12:0) | PW002548 | | Cholesterol biosynthesis and metabolism CE(14:0) | PW002544 | | Cholesterol biosynthesis and metabolism CE(16:0) | PW002550 | | Cholesterol biosynthesis and metabolism CE(18:0) | PW002551 | |
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KEGG Pathways | |
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SMPDB Reactions | |
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KEGG Reactions | |
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Metabolites | YMDB ID | Name | View |
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YMDB00219 | Pyrophosphate | Show | YMDB00229 | Farnesyl pyrophosphate | Show | YMDB00325 | 2-trans,6-trans-farnesyl diphosphate | Show | YMDB00426 | NADPH | Show | YMDB00427 | NADP | Show | YMDB00769 | squalene | Show | YMDB00843 | presqualene diphosphate | Show | YMDB00862 | hydron | Show |
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GO Classification | Component |
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cell part | membrane part | intrinsic to membrane | integral to membrane | Function |
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farnesyl-diphosphate farnesyltransferase activity | catalytic activity | transferase activity | transferase activity, transferring alkyl or aryl (other than methyl) groups | prenyltransferase activity | farnesyltranstransferase activity | Process |
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metabolic process | biosynthetic process | primary metabolic process | lipid metabolic process | lipid biosynthetic process |
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Gene Properties |
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Chromosome Location | chromosome 8 |
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Locus | YHR190W |
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Gene Sequence | >1335 bp
ATGGGAAAGCTATTACAATTGGCATTGCATCCGGTCGAGATGAAGGCAGCTTTGAAGCTG
AAGTTTTGCAGAACACCGCTATTCTCCATCTATGATCAGTCCACGTCTCCATATCTCTTG
CACTGTTTCGAACTGTTGAACTTGACCTCCAGATCGTTTGCTGCTGTGATCAGAGAGCTG
CATCCAGAATTGAGAAACTGTGTTACTCTCTTTTATTTGATTTTAAGGGCTTTGGATACC
ATCGAAGACGATATGTCCATCGAACACGATTTGAAAATTGACTTGTTGCGTCACTTCCAC
GAGAAATTGTTGTTAACTAAATGGAGTTTCGACGGAAATGCCCCCGATGTGAAGGACAGA
GCCGTTTTGACAGATTTCGAATCGATTCTTATTGAATTCCACAAATTGAAACCAGAATAT
CAAGAAGTCATCAAGGAGATCACCGAGAAAATGGGTAATGGTATGGCCGACTACATCTTG
GATGAAAATTACAACTTGAATGGGTTGCAAACCGTCCACGACTACGACGTGTACTGTCAC
TACGTAGCTGGTTTGGTCGGTGATGGTTTGACCCGTTTGATTGTCATTGCCAAGTTTGCC
AACGAATCTTTGTATTCTAATGAGCAATTGTATGAAAGCATGGGTCTTTTCCTACAAAAA
ACCAACATCATCAGAGACTACAATGAAGATTTGGTCGATGGTAGATCCTTCTGGCCCAAG
GAAATCTGGTCACAATACGCTCCTCAGTTGAAGGACTTCATGAAACCTGAAAACGAACAA
CTGGGGTTGGACTGTATAAACCACCTCGTCTTAAACGCATTGAGTCATGTTATCGATGTG
TTGACTTATTTGGCCAGTATCCACGAGCAATCCACTTTCCAATTTTGTGCCATTCCCCAA
GTTATGGCCATTGCAACCTTGGCTTTGGTATTCAACAACCGTGAAGTGCTACATGGCAAT
GTAAAGATTCGTAAGGGTACTACCTGCTATTTAATTTTGAAATCAAGGACTTTGCGTGGC
TGTGTCGAGATTTTTGACTATTACTTACGTGATATCAAATCTAAATTGGCTGTGCAAGAT
CCAAATTTCTTAAAATTGAACATTCAAATCTCCAAGATCGAACAATTCATGGAAGAAATG
TACCAGGATAAATTACCTCCTAACGTGAAGCCAAATGAAACTCCAATTTTCTTGAAAGTT
AAAGAAAGATCCAGATACGATGATGAATTGGTCCCAACCCAACAAGAAGAAGAGTACAAG
TTCAATATGGTTTTATCTATCATCTTGTCCGTTCTTCTTGGGTTTTATTATATATACACT
TTACACAGAGCGTGA |
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Protein Properties |
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Pfam Domain Function | |
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Protein Residues | 444 |
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Protein Molecular Weight | 51719.39844 |
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Protein Theoretical pI | 5.75 |
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Signalling Regions | |
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Transmembrane Regions | |
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Protein Sequence | >Squalene synthase
MGKLLQLALHPVEMKAALKLKFCRTPLFSIYDQSTSPYLLHCFELLNLTSRSFAAVIREL
HPELRNCVTLFYLILRALDTIEDDMSIEHDLKIDLLRHFHEKLLLTKWSFDGNAPDVKDR
AVLTDFESILIEFHKLKPEYQEVIKEITEKMGNGMADYILDENYNLNGLQTVHDYDVYCH
YVAGLVGDGLTRLIVIAKFANESLYSNEQLYESMGLFLQKTNIIRDYNEDLVDGRSFWPK
EIWSQYAPQLKDFMKPENEQLGLDCINHLVLNALSHVIDVLTYLAGIHEQSTFQFCAIPQ
VMAIATLALVFNNREVLHGNVKIRKGTTCYLILKSRTLRGCVEIFDYYLRDIKSKLAVQD
PNFLKLNIQISKIEQFMEEMYQDKLPPNVKPNETPIFLKVKERSRYDDELVPTQQEEEYK
FNMVLSIILSVLLGFYYIYTLHRA |
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References |
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External Links | |
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General Reference | - Fegueur, M., Richard, L., Charles, A. D., Karst, F. (1991). "Isolation and primary structure of the ERG9 gene of Saccharomyces cerevisiae encoding squalene synthetase." Curr Genet 20:365-372.1807826
- Jennings, S. M., Tsay, Y. H., Fisch, T. M., Robinson, G. W. (1991). "Molecular cloning and characterization of the yeast gene for squalene synthetase." Proc Natl Acad Sci U S A 88:6038-6042.2068081
- Johnston, M., Andrews, S., Brinkman, R., Cooper, J., Ding, H., Dover, J., Du, Z., Favello, A., Fulton, L., Gattung, S., et, a. l. .. (1994). "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Science 265:2077-2082.8091229
- Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
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