3-hydroxyisobutyryl-CoA hydrolase, mitochondrial (P28817)

Identification
Name3-hydroxyisobutyryl-CoA hydrolase, mitochondrial
Synonyms
  • 3-hydroxyisobutyryl-coenzyme A hydrolase
  • HIB-CoA hydrolase
  • HIBYL-CoA-H
Gene NameEHD3
Enzyme Class
Biological Properties
General FunctionInvolved in catalytic activity
Specific FunctionHydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA. Has an indirect role in endocytic membrane trafficking. May have a function in protein biosynthesis in mitochondrial small ribosomal subunit
Cellular LocationMitochondrion
SMPDB Pathways
Fatty acid metabolismPW002460 ThumbThumb?image type=greyscaleThumb?image type=simple
beta-Alanine metabolismPW002381 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
Fatty acid metabolismec00071 Map00071
Propanoate metabolismec00640 Map00640
Valine, leucine and isoleucine degradationec00280 Map00280
beta-Alanine metabolismec00410 Map00410
SMPDB Reactions
(S)-3-hydroxypalmitoyl-CoA ↔ hexadec-2-enoyl-CoA + water
(S)-3-hydroxytetradecanoyl-CoA ↔ (2E)-Tetradecenoyl-CoA + water
(S)-3-hydroxydecanoyl-CoA ↔ (2E)-Decenoyl-CoA + water
(S)-Hydroxyoctanoyl-CoA ↔ (2E)-Octenoyl-CoA + water
(S)-Hydroxyhexanoyl-CoA ↔ trans-2-Hexenoyl-CoA + water
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00045Coenzyme AShow
YMDB00053(S)-3-Hydroxyisobutyryl-CoAShow
YMDB00215(2E)-Dodecenoyl-CoAShow
YMDB00337(S)-3-Hydroxyisobutyric acidShow
YMDB00464(S)-3-hydroxydecanoyl-CoAShow
YMDB00465(S)-3-hydroxylauroyl-CoAShow
YMDB00468(S)-3-hydroxypalmitoyl-CoAShow
YMDB00469hexadec-2-enoyl-CoAShow
YMDB00473(S)-3-hydroxytetradecanoyl-CoAShow
YMDB00890waterShow
YMDB161333-Hydroxybutyryl-CoAShow
YMDB16143Crotonoyl-CoAShow
YMDB16167(S)-Hydroxyoctanoyl-CoAShow
YMDB16169(S)-Hydroxyhexanoyl-CoAShow
YMDB16171trans-2-Hexenoyl-CoAShow
YMDB16172(2E)-Tetradecenoyl-CoAShow
YMDB16173(2E)-Decenoyl-CoAShow
YMDB16174(2E)-Octenoyl-CoAShow
GO Classification
Component
Not Available
Function
catalytic activity
Process
metabolic process
Gene Properties
Chromosome Locationchromosome 4
LocusYDR036C
Gene Sequence>1503 bp ATGCTCAGAAATACGCTAAAATGTGCCCAATTGTCGTCTAAATACGGATTTAAAACTACC ACACGTACATTCATGACTACTCAACCCCAGCTAAATGTCACCGACGCACCACCTGTGCTA TTTACCGTTCAAGATACAGCTAGAGTTATCACGCTAAATAGGCCCAAAAAGCTCAATGCT TTGAACGCCGAAATGTCAGAATCCATGTTCAAGACTTTGAACGAGTATGCAAAGAGCGAT ACTACAAACTTAGTCATTTTAAAGTCATCCAACCGACCACGTTCGTTCTGTGCTGGTGGT GATGTAGCTACTGTGGCAATATTCAATTTTAACAAAGAATTTGCCAAGTCCATCAAATTT TTTACTGATGAATATTCTTTGAATTTTCAAATAGCAACTTACTTGAAACCAATTGCTACC TTCATGGACGGTATCACCATGGGTGGCGGCGTTGGTCTATCCATTCACACGCCCTTTAGA ATTGCTACAGAAAACACCAAATGGGCCATGCCCGAGATGGACATTGGTTTTTTTCCAGAT GTAGGCTCAACTTTTGCTCTCCCTAGAATCGTGACATTGGCTAACTCAAACTCACAAATG GCCCTGTATCTATGTCTTACAGGAGAAGTAGTCACAGGAGCAGACGCTTATATGCTCGGC TTAGCGTCTCATTACGTCAGTAGTGAAAATTTAGATGCTTTGCAGAAAAGATTAGGTGAA ATTAGCCCCCCTTTTAATAACGATCCACAATCTGCATACTTCTTCGGGATGGTTAACGAA TCCATCGACGAATTCGTATCACCATTACCAAAAGATTATGTTTTCAAGTATTCTAACGAG AAATTAAACGTTATTGAAGCCTGTTTTAACTTGTCTAAAAATGGTACTATTGAAGACATA ATGAATAACTTACGTCAATATGAAGGTTCTGCGGAAGGTAAGGCTTTCGCACAAGAAATC AAAACGAAATTGTTAACCAAGTCACCATCCTCTCTTCAAATCGCCTTGAGATTGGTGCAA GAGAATTCCAGAGATCACATAGAATCTGCTATCAAAAGAGACTTATACACAGCGGCTAAC ATGTGCATGAACCAGGACTCTTTGGTGGAATTCTCTGAAGCCACAAAGCATAAACTTATT GATAAACAAAGGGTCCCGTATCCATGGACAAAGAAGGAACAGTTATTTGTATCTCAGTTG ACATCTATCACATCTCCTAAACCATCGCTACCAATGTCATTACTAAGAAATACCTCGAAT GTTACTTGGACTCAATATCCCTACCATTCTAAATACCAATTGCCTACAGAACAGGAAATC GCTGCGTATATTGAAAAGAGAACGAATGATGACACTGGCGCCAAAGTTACCGAAAGAGAA GTACTAAATCACTTTGCCAATGTGATTCCTTCTAGAAGAGGGAAACTGGGTATCCAATCG CTATGTAAAATTGTTTGTGAAAGAAAATGTGAAGAAGTTAACGATGGCTTAAGATGGAAA TAA
Protein Properties
Pfam Domain Function
Protein Residues500
Protein Molecular Weight56287.89844
Protein Theoretical pI8.5
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>3-hydroxyisobutyryl-CoA hydrolase, mitochondrial MLRNTLKCAQLSSKYGFKTTTRTFMTTQPQLNVTDAPPVLFTVQDTARVITLNRPKKLNA LNAEMSESMFKTLNEYAKSDTTNLVILKSSNRPRSFCAGGDVATVAIFNFNKEFAKSIKF FTDEYSLNFQIATYLKPIVTFMDGITMGGGVGLSIHTPFRIATENTKWAMPEMDIGFFPD VGSTFALPRIVTLANSNSQMALYLCLTGEVVTGADAYMLGLASHYVSSENLDALQKRLGE ISPPFNNDPQSAYFFGMVNESIDEFVSPLPKDYVFKYSNEKLNVIEACFNLSKNGTIEDI MNNLRQYEGSAEGKAFAQEIKTKLLTKSPSSLQIALRLVQENSRDHIESAIKRDLYTAAN MCMNQDSLVEFSEATKHKLIDKQRVPYPWTKKEQLFVSQLTSITSPKPSLPMSLLRNTSN VTWTQYPYHSKYQLPTEQEIAAYIEKRTNDDTGAKVTEREVLNHFANVIPSRRGKLGIQS LCKIVCERKCEEVNDGLRWK
References
External Links
ResourceLink
Saccharomyces Genome Database EHD3
Uniprot IDP28817
Uniprot NameHIBCH_YEAST
GenBank Gene IDAY723769
Genebank Protein ID51830224
General Reference
  • Jacq, C., Alt-Morbe, J., Andre, B., Arnold, W., Bahr, A., Ballesta, J. P., Bargues, M., Baron, L., Becker, A., Biteau, N., Blocker, H., Blugeon, C., Boskovic, J., Brandt, P., Bruckner, M., Buitrago, M. J., Coster, F., Delaveau, T., del Rey, F., Dujon, B., Eide, L. G., Garcia-Cantalejo, J. M., Goffeau, A., Gomez-Peris, A., Zaccaria, P., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Nature 387:75-78.9169867
  • Hu, Y., Rolfs, A., Bhullar, B., Murthy, T. V., Zhu, C., Berger, M. F., Camargo, A. A., Kelley, F., McCarron, S., Jepson, D., Richardson, A., Raphael, J., Moreira, D., Taycher, E., Zuo, D., Mohr, S., Kane, M. F., Williamson, J., Simpson, A., Bulyk, M. L., Harlow, E., Marsischky, G., Kolodner, R. D., LaBaer, J. (2007). "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Genome Res 17:536-543.17322287
  • Mirande, M., Waller, J. P. (1988). "The yeast lysyl-tRNA synthetase gene. Evidence for general amino acid control of its expression and domain structure of the encoded protein." J Biol Chem 263:18443-18451.2903861
  • Reinders, J., Wagner, K., Zahedi, R. P., Stojanovski, D., Eyrich, B., van der Laan, M., Rehling, P., Sickmann, A., Pfanner, N., Meisinger, C. (2007). "Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase." Mol Cell Proteomics 6:1896-1906.17761666
  • Wiederkehr, A., Meier, K. D., Riezman, H. (2001). "Identification and characterization of Saccharomyces cerevisiae mutants defective in fluid-phase endocytosis." Yeast 18:759-773.11378903
  • Hiltunen, J. K., Mursula, A. M., Rottensteiner, H., Wierenga, R. K., Kastaniotis, A. J., Gurvitz, A. (2003). "The biochemistry of peroxisomal beta-oxidation in the yeast Saccharomyces cerevisiae." FEMS Microbiol Rev 27:35-64.12697341
  • Huh, W. K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., O'Shea, E. K. (2003). "Global analysis of protein localization in budding yeast." Nature 425:686-691.14562095
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Samanta, M. P., Liang, S. (2003). "Predicting protein functions from redundancies in large-scale protein interaction networks." Proc Natl Acad Sci U S A 100:12579-12583.14566057
  • Sickmann, A., Reinders, J., Wagner, Y., Joppich, C., Zahedi, R., Meyer, H. E., Schonfisch, B., Perschil, I., Chacinska, A., Guiard, B., Rehling, P., Pfanner, N., Meisinger, C. (2003). "The proteome of Saccharomyces cerevisiae mitochondria." Proc Natl Acad Sci U S A 100:13207-13212.14576278
  • Reinders, J., Zahedi, R. P., Pfanner, N., Meisinger, C., Sickmann, A. (2006). "Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics." J Proteome Res 5:1543-1554.16823961