CTP synthase 1 (P28274) - Yeast Metabolome Database

Identification

Name

CTP synthase 1

Synonyms

CTP synthetase 1
UTP--ammonia ligase 1

Gene Name

URA7

Enzyme Class

6.3.4.2

Biological Properties

General Function

Involved in CTP synthase activity

Specific Function

Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

Cellular Location

Not Available

SMPDB Pathways

Pyrimidine metabolism

PW002469

KEGG Pathways

Pyrimidine metabolism

ec00240

SMPDB Reactions

Not Available

KEGG Reactions

Uridine triphosphate + Adenosine triphosphate + water + L-Glutamine → phosphate + L-Glutamic acid + hydron + ADP + Cytidine triphosphate

Uridine triphosphate + Adenosine triphosphate + Ammonium → phosphate + hydron + ADP + Cytidine triphosphate

Metabolites

YMDB ID	Name	View
YMDB00002	L-Glutamine	Show
YMDB00091	Ammonia	Show
YMDB00109	Adenosine triphosphate	Show
YMDB00271	L-Glutamic acid	Show
YMDB00279	Cytidine triphosphate	Show
YMDB00326	Uridine triphosphate	Show
YMDB00423	Ammonium	Show
YMDB00862	hydron	Show
YMDB00890	water	Show
YMDB00907	phosphate	Show
YMDB00914	ADP	Show

GO Classification

Component
Not Available
Function
ligase activity
ligase activity, forming carbon-nitrogen bonds
CTP synthase activity
catalytic activity
Process
nitrogen compound metabolic process
cellular nitrogen compound metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleobase, nucleoside and nucleotide metabolic process
nucleoside phosphate metabolic process
nucleotide metabolic process
pyrimidine nucleotide metabolic process
pyrimidine nucleotide biosynthetic process
metabolic process

Gene Properties

Chromosome Location

chromosome 2

Locus

YBL039C

Gene Sequence

>1740 bp ATGAAGTACGTTGTTGTTTCAGGTGGTGTCATTTCGGGTATCGGTAAAGGTGTTCTTGCA TCTTCAACTGGTATGCTAATGAAAACCCTCGGTTTAAAGGTTACGTCAATTAAAATTGAC CCTTATATGAACATTGATGCAGGTACTATGTCTCCTTTGGAACATGGTGAATGTTTCGTG CTTGACGACGGTGGTGAAACAGATTTGGACTTGGGTAACTACGAGAGATACCTTGGAGTT ACTTTGACTAAAGATCATAACATTACTACTGGTAAAATATACTCGCACGTTATTGCTAAG GAAAGAAAAGGTGACTATTTAGGCAAAACCGTGCAAATTGTCCCTCATTTGACCAACGCT ATCCAAGATTGGATTGAGCGCGTGGCCAAAATTCCTGTAGATGACACCGGTATGGAACCA GATGTTTGTATCATTGAATTGGGTGGGACAGTTGGTGACATTGAAAGTGCTCCTTTTGTG GAGGCACTAAGACAATTCCAATTTAAAGTTGGAAAGGAAAATTTTGCCTTGATACACGTT TCTCTAGTTCCTGTCATCCACGGTGAACAAAAAACTAAGCCAACTCAAGCTGCCATCAAA GGTTTGAGGTCTCTTGGTTTAGTTCCAGATATGATCGCCTGTAGATGTAGTGAAACTTTG GATAAACCAACAATCGATAAGATTGCCATGTTTTGTCATGTGGGGCCTGAACAGGTGGTT AACGTTCACGATGTTAATTCTACTTATCACGTCCCATTGTTGCTACTGGAACAAAAGATG ATTGATTACTTACACGCCAGATTAAAGTTAGATGAAATATCACTTACAGAAGAAGAAAAA CAAAGAGGCCTGGAATTATTGTCTAAATGGAAGGCCACAACAGGTAACTTCGATGAATCC ATGGAAACAGTGAAGATTCGTTTGGTTGGGAAATACACCAACTTGAAAGATTCTTATCTA TCAGTGATAAAAGCATTGGAACATTCATCCATGAAGTGTCGTCGTAAGTTAGACATTAAA TGGGTGGAAGCTACTGATTTGGAACCTGAAGCACAAGAAAGCAACAAAACTAAATTTCAT GAAGCATGGAACATGGTCAGTACCGCAGACGGTATCTTAATCCCCGGTGGTTTTGGTGTT AGAGGTACTGAAGGTATGGTTTTGGCTGCTAGATGGGCTCGTGAAAATCACATTCCATTC TTAGGGGTTTGCCTGGGTTTACAAATTGCCACCATTGAATTTACACGTAGTTCGTTGGGA AGAAAAGACAGTCATTCGGCAGAATTTTATCCAGACATTGATGAAAAAAATCACGTTGTC GTTTTCATGCCTGAAATTGACAAGGAAACCATGGGGGGCTCAATGAGATTAGGTTTGAGA CCAACATTTTTCCAAAATGAAACTGAATGGAGTCAAATCAAGAAGTTATACGGTGACGTC TCCGAAGTCCATGAAAGACACCGTCACCGTTACGAAATCAATCCAAAGATGGTTGATGAA TTGGAAAACAACGGGTTGATCTTTGTAGGTAAGGACGACACTGGAAAGCGTTGTGAGATT TTGGAATTAAAGAATCACCCATACTATATTGCTACTCAATATCACCCAGAATACACATCA AAGGTCTTAGATCCATCCAAACCATTTTTGGGTTTAGTTGCTGCTTCCGCTGGTATCCTT CAAGATGTCATTGAAGGTAAGTACGATCTTGAGGCCGGCGAAAACAAATTCAACTTTTAA

Protein Properties

Pfam Domain Function

GATase (PF00117 )
CTP_synth_N (PF06418 )

Protein Residues

579

Protein Molecular Weight

64709.80078

Protein Theoretical pI

5.91

Signalling Regions

None

Transmembrane Regions

None

Protein Sequence

>CTP synthase 1 MKYVVVSGGVISGIGKGVLASSTGMLMKTLGLKVTSIKIDPYMNIDAGTMSPLEHGECFV LDDGGETDLDLGNYERYLGVTLTKDHNITTGKIYSHVIAKERKGDYLGKTVQIVPHLTNA IQDWIERVAKIPVDDTGMEPDVCIIELGGTVGDIESAPFVEALRQFQFKVGKENFALIHV SLVPVIHGEQKTKPTQAAIKGLRSLGLVPDMIACRCSETLDKPTIDKIAMFCHVGPEQVV NVHDVNSTYHVPLLLLEQKMIDYLHARLKLDEISLTEEEKQRGLELLSKWKATTGNFDES METVKIALVGKYTNLKDSYLSVIKALEHSSMKCRRKLDIKWVEATDLEPEAQESNKTKFH EAWNMVSTADGILIPGGFGVRGTEGMVLAARWARENHIPFLGVCLGLQIATIEFTRSVLG RKDSHSAEFYPDIDEKNHVVVFMPEIDKETMGGSMRLGLRPTFFQNETEWSQIKKLYGDV SEVHERHRHRYEINPKMVDELENNGLIFVGKDDTGKRCEILELKNHPYYIATQYHPEYTS KVLDPSKPFLGLVAASAGILQDVIEGKYDLEAGENKFNF

References

External Links

Resource	Link
Saccharomyces Genome Database	URA7
Uniprot ID	P28274
Uniprot Name	URA7_YEAST
GenBank Gene ID	X53995
Genebank Protein ID	440583

General Reference

Ozier-Kalogeropoulos, O., Fasiolo, F., Adeline, M. T., Collin, J., Lacroute, F. (1991). "Cloning, sequencing and characterization of the Saccharomyces cerevisiae URA7 gene encoding CTP synthetase." Mol Gen Genet 231:7-16.1753946
De Wergifosse, P., Jacques, B., Jonniaux, J. L., Purnelle, B., Skala, J., Goffeau, A. (1994). "The sequence of a 22.4 kb DNA fragment from the left arm of yeast chromosome II reveals homologues to bacterial proline synthetase and murine alpha-adaptin, as well as a new permease and a DNA-binding protein." Yeast 10:1489-1496.7871888
Feldmann, H., Aigle, M., Aljinovic, G., Andre, B., Baclet, M. C., Barthe, C., Baur, A., Becam, A. M., Biteau, N., Boles, E., Brandt, T., Brendel, M., Bruckner, M., Bussereau, F., Christiansen, C., Contreras, R., Crouzet, M., Cziepluch, C., Demolis, N., Delaveau, T., Doignon, F., Domdey, H., Dusterhus, S., Dubois, E., Dujon, B., El Bakkoury, M., Entian, K. D., Feurmann, M., Fiers, W., Fobo, G. M., Fritz, C., Gassenhuber, H., Glandsdorff, N., Goffeau, A., Grivell, L. A., de Haan, M., Hein, C., Herbert, C. J., Hollenberg, C. P., Holmstrom, K., Jacq, C., Jacquet, M., Jauniaux, J. C., Jonniaux, J. L., Kallesoe, T., Kiesau, P., Kirchrath, L., Kotter, P., Korol, S., Liebl, S., Logghe, M., Lohan, A. J., Louis, E. J., Li, Z. Y., Maat, M. J., Mallet, L., Mannhaupt, G., Messenguy, F., Miosga, T., Molemans, F., Muller, S., Nasr, F., Obermaier, B., Perea, J., Pierard, A., Piravandi, E., Pohl, F. M., Pohl, T. M., Potier, S., Proft, M., Purnelle, B., Ramezani Rad, M., Rieger, M., Rose, M., Schaaff-Gerstenschlager, I., Scherens, B., Schwarzlose, C., Skala, J., Slonimski, P. P., Smits, P. H., Souciet, J. L., Steensma, H. Y., Stucka, R., Urrestarazu, A., van der Aart, Q. J., van Dyck, L., Vassarotti, A., Vetter, I., Vierendeels, F., Vissers, S., Wagner, G., de Wergifosse, P., Wolfe, K. H., Zagulski, M., Zimmermann, F. K., Mewes, H. W., Kleine, K. (1994). "Complete DNA sequence of yeast chromosome II." EMBO J 13:5795-5809.7813418
Yang, W. L., McDonough, V. M., Ozier-Kalogeropoulos, O., Adeline, M. T., Flocco, M. T., Carman, G. M. (1994). "Purification and characterization of CTP synthetase, the product of the URA7 gene in Saccharomyces cerevisiae." Biochemistry 33:10785-10793.8075080
Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
Peng, J., Schwartz, D., Elias, J. E., Thoreen, C. C., Cheng, D., Marsischky, G., Roelofs, J., Finley, D., Gygi, S. P. (2003). "A proteomics approach to understanding protein ubiquitination." Nat Biotechnol 21:921-926.12872131