Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (P14742)

Identification
NameGlucosamine--fructose-6-phosphate aminotransferase [isomerizing]
Synonyms
  • GFAT
  • D-fructose-6-phosphate amidotransferase
  • Hexosephosphate aminotransferase
Gene NameGFA1
Enzyme Class
Biological Properties
General FunctionInvolved in metabolic process
Specific FunctionInvolved in amino sugar synthesis (formation of chitin, supplies the amino sugars of asparagine-linked oligosaccharides of glycoproteins)
Cellular LocationNot Available
SMPDB Pathways
Amino sugar and nucleotide sugar metabolismPW002413 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
Alanine, aspartate and glutamate metabolismec00250 Map00250
Amino sugar and nucleotide sugar metabolismec00520 Map00520
SMPDB Reactions
Beta-D-Fructose 6-phosphate + L-Glutamine → Glucosamine 6-phosphate + L-Glutamic acid
KEGG Reactions
Fructose 6-phosphate + L-Glutamine → L-Glutamic acid + Glucosamine 6-phosphate
Metabolites
YMDB IDNameView
YMDB00002L-GlutamineShow
YMDB00044Glucosamine 6-phosphateShow
YMDB00078Fructose 6-phosphateShow
YMDB00271L-Glutamic acidShow
YMDB16176Beta-D-Fructose 6-phosphateShow
GO Classification
Component
intracellular part
cytoplasm
cell part
Function
transaminase activity
glutamine-fructose-6-phosphate transaminase (isomerizing) activity
catalytic activity
transferase activity
binding
carbohydrate binding
sugar binding
transferase activity, transferring nitrogenous groups
Process
primary metabolic process
carbohydrate metabolic process
carbohydrate biosynthetic process
metabolic process
Gene Properties
Chromosome Locationchromosome 11
LocusYKL104C
Gene Sequence>2154 bp ATGTGTGGTATCTTTGGTTACTGCAATTATCTAGTGGAAAGATCCAGAGGAGAAATTATC GACACCTTAGTGGATGGTTTACAAAGATTAGAATATAGAGGCTATGATTCCACCGGTATT GCTATCGATGGTGACGAAGCTGATTCTACTTTCATCTATAAGCAAATCGGTAAAGTGAGT GCTTTGAAAGAGGAGATTACTAAGCAAAATCCGAACAGAGACGTTACTTTTGTCTCTCAT TGTGGTATTGCGCATACTAGATGGGCTACTCACGGTCGACCAGAACAAGTTAACTGTCAC CCTCAAAGATCTGACCCAGAAGACCAATTTGTGGTCGTTCATAATGGTATCATCACAAAT TTTAGAGAACTGAAGACTCTTTTAATTAACAAAGGTTATAAATTCGAAAGTGATACCGAT ACCGAGTGTATTGCTAAACTATATTTGCATTTATACAATACAAATTTACAAAATGGGCAT GACTTAGATTTCCACGAATTAACCAAGCTAGTTCTTTTAGAACTAGAAGGTTCATACGGG TTATTATGTAAATCTTGTCACTATCCTAATGAGGTTATCGCCACTAGAAAAGGGTCCCCT TTACTGATTGGTGTCAAATCTGAAAAAAAACTAAAAGTCGACTTCGTGGATGTGGAATTT CCCGAAGAAAACGCTGGTCAACCGGAAATTCCATTGAAATCTAACAACAAATCATTTGGC TTGGGCCCAAAGAAAGCTCGTGAATTTGAAGCTGGTTCCCAAAATGCCAATTTACTACCA ATTGCCGCCAATGAATTTAACTTGAGACATTCTCAATCCAGGGCTTTCCTATCAGAAGAT GGATCTCCAACACCGGTGGAATTTTTTGTTTCTTCGGATGCGGCATCTGTTGTTAAACAT ACCAAGAAGGTGCTATTTTTAGAAGATGACGATTTGGCTCATATTTACGATGGTGAGTTA CATATTCATAGATCTAGAAGAGAAGTAGGCGCATCAATGACAAGGTCCATTCAAACTTTA GAGATGGAGTTAGCTCAGATCATGAAGGGCCCTTACGACCATTTTATGCAAAAGGAAATC TATGAGCAACCAGAATCTACTTTCAATACTATGAGAGGTAGAATCGACTATGAAAATAAT AAAGTGATATTGGGTGGTTTAAAGGCATGGTTACCAGTTGTCAGAAGAGCACGGAGACTG ATCATGATCGCATGCGGTACTTCTTATCATTCATGTTTGGCTACTCGTGCTATCTTCGAA GAATTATCAGATATCCCAGTTAGTGTGGAATTAGCGTCTGACTTTCTGGACAGAAAATGC CCTGTCTTCAGAGACGATGTATGCGTGTTTGTTTCACAAAGTGGTGAAACTGCGGATACC ATGCTGGCTCTAAATTATTGTTTAGAAAGAGGAGCCTTAACTGTCGGAATTGTTAACAGT GTTGGTTCTTCTATCTCTCGTGTCACCCACTGTGGTGTTCATATTAACGCTGGTCCTGAA ATTGGTGTTGCCTCTACAAAAGCTTATACTTCCCAGTATATTGCCTTAGTGATGTTTGCT CTATCGCTGTCAGATGACCGTGTATCGAAAATAGACAGAAGAATTGAAATCATTCAAGGC TTGAAGTTAATCCCGGGCCAAATTAAGCAGGTATTAAAGCTGGAACCAAGAATAAAAAAG CTCTGTGCGACTGAATTAAAGGATCAAAAATCTCTATTGTTATTGGGTAGAGGTTACCAA TTTGCTGCTGCTCTGGAAGGTGCTTTGAAGATCAAAGAAATTTCTTATATGCATTCTGAA GGTGTTTTGGCAGGTGAGTTGAAGCACGGTGTCTTGGCCTTGGTGGACGAAAACTTGCCA ATCATTGCTTTTGGTACCAGAGACTCTCTATTCCCTAAAGTAGTTTCCTCTATTGAGCAA GTTACTGCAAGAAAGGGCCATCCAATTATTATTTGTAACGAAAATGATGAAGTGTGGGCG CAAAAATCTAAATCAATCGACCTGCAAACCTTAGAAGTTCCACAAACTGTTGATTGTTTA CAAGGTCTAATTAATATTATTCCATTACAACTAATGTCATATTGGTTGGCTGTTAATAAA GGGATTGATGTTGATTTTCCAAGAAACTTGGCTAAATCTGTTACCGTCGAATAA
Protein Properties
Pfam Domain Function
Protein Residues717
Protein Molecular Weight80046.0
Protein Theoretical pI6.4
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] MCGIFGYCNYLVERSRGEIIDTLVDGLQRLEYRGYDSTGIAIDGDEADSTFIYKQIGKVS ALKEEITKQNPNRDVTFVSHCGIAHTRWATHGRPEQVNCHPQRSDPEDQFVVVHNGIITN FRELKTLLINKGYKFESDTDTECIAKLYLHLYNTNLQNGHDLDFHELTKLVLLELEGSYG LLCKSCHYPNEVIATRKGSPLLIGVKSEKKLKVDFVDVEFPEENAGQPEIPLKSNNKSFG LGPKKAREFEAGSQNANLLPIAANEFNLRHSQSRAFLSEDGSPTPVEFFVSSDAASVVKH TKKVLFLEDDDLAHIYDGELHIHRSRREVGASMTRSIQTLEMELAQIMKGPYDHFMQKEI YEQPESTFNTMRGRIDYENNKVILGGLKAWLPVVRRARRLIMIACGTSYHSCLATRAIFE ELSDIPVSVELASDFLDRKCPVFRDDVCVFVSQSGETADTMLALNYCLERGALTVGIVNS VGSSISRVTHCGVHINAGPEIGVASTKAYTSQYIALVMFALSLSDDRVSKIDRRIEIIQG LKLIPGQIKQVLKLEPRIKKLCATELKDQKSLLLLGRGYQFAAALEGALKIKEISYMHSE GVLAGELKHGVLALVDENLPIIAFGTRDSLFPKVVSSIEQVTARKGHPIIICNENDEVWA QKSKSIDLQTLEVPQTVDCLQGLINIIPLQLMSYWLAVNKGIDVDFPRNLAKSVTVE
References
External Links
ResourceLink
Saccharomyces Genome Database GFA1
Uniprot IDP14742
Uniprot NameGFA1_YEAST
GenBank Gene IDX71133
Genebank Protein ID431212
General Reference
  • Watzele, G., Tanner, W. (1989). "Cloning of the glutamine:fructose-6-phosphate amidotransferase gene from yeast. Pheromonal regulation of its transcription." J Biol Chem 264:8753-8758.2656689
  • Cheret, G., Pallier, C., Valens, M., Diagnan-Fornier, B., Fukuhara, H., Bolotin-Fukuhara, M., Sor, F. (1993). "The DNA sequence analysis of the HAP4-LAP4 region on chromosome XI of Saccharomyces cerevisiae suggests the presence of a second aspartate aminotransferase gene in yeast." Yeast 9:1259-1265.8109175
  • Dujon, B., Alexandraki, D., Andre, B., Ansorge, W., Baladron, V., Ballesta, J. P., Banrevi, A., Bolle, P. A., Bolotin-Fukuhara, M., Bossier, P., et, a. l. .. (1994). "Complete DNA sequence of yeast chromosome XI." Nature 369:371-378.8196765
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Gruhler, A., Olsen, J. V., Mohammed, S., Mortensen, P., Faergeman, N. J., Mann, M., Jensen, O. N. (2005). "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Mol Cell Proteomics 4:310-327.15665377
  • Smolka, M. B., Albuquerque, C. P., Chen, S. H., Zhou, H. (2007). "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Proc Natl Acad Sci U S A 104:10364-10369.17563356
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956