Ribose-phosphate pyrophosphokinase 5 (Q12265)

Identification

Name

Ribose-phosphate pyrophosphokinase 5

Synonyms

Phosphoribosyl pyrophosphate synthase 5

Gene Name

PRS5

Enzyme Class

2.7.6.1

Biological Properties

General Function

Involved in magnesium ion binding

Specific Function

5-phosphoribose 1-diphosphate synthase involved in nucleotide, histidine, and tryptophan biosynthesis. Active in heteromultimeric complexes with other 5-phosphoribose 1- diphosphate synthases (PRS2, PRS3, PRS4 and PRS5)

Cellular Location

Cytoplasm

SMPDB Pathways

Histidine Biosynthesis

PW002418

KEGG Pathways

Pentose phosphate pathway	ec00030
Purine metabolism	ec00230

SMPDB Reactions

D-Ribose 5-phosphate + Adenosine triphosphate → Adenosine monophosphate + hydron + Phosphoribosyl pyrophosphate

KEGG Reactions

Adenosine triphosphate + alpha-D-ribose 5-phosphate ↔ Adenosine monophosphate + hydron + Phosphoribosyl pyrophosphate

Metabolites

YMDB ID	Name	View
YMDB00097	Adenosine monophosphate	Show
YMDB00109	Adenosine triphosphate	Show
YMDB00119	D-Ribose 5-phosphate	Show
YMDB00402	Phosphoribosyl pyrophosphate	Show
YMDB00650	alpha-D-ribose 5-phosphate	Show
YMDB00862	hydron	Show

GO Classification

Component
Not Available
Function
binding
ion binding
cation binding
metal ion binding
catalytic activity
magnesium ion binding
transferase activity
transferase activity, transferring phosphorus-containing groups
diphosphotransferase activity
ribose phosphate diphosphokinase activity
Process
ribonucleoside monophosphate biosynthetic process
nucleoside phosphate metabolic process
nucleotide metabolic process
biosynthetic process
nucleoside metabolic process
cellular biosynthetic process
metabolic process
nucleoside monophosphate metabolic process
nucleoside monophosphate biosynthetic process
nitrogen compound metabolic process
nucleotide biosynthetic process
cellular nitrogen compound metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleobase, nucleoside and nucleotide metabolic process

Gene Properties

Chromosome Location

chromosome 15

Locus

YOL061W

Gene Sequence

>1491 bp ATGTCAATGAGTAATATTGTTGTTTTTGGAGGGGACTCGCACCCCGAGTTAGTTACTAAG ATCTGTGAAAATTTGGACATTCACCCATCGAAAGTAGAATTAGGGAAGTTTTCTAATGGG GAAACGAACATTGCTCTTCGCGAATCTGTTCGTGAAAAGGATGTATATATCATCCAGAGT GGTTGTGGCCAGGTGAACGACACGTTCATGCAGTTGCTGATTTTAATCAGTGCCTGCAAG TCCGCTTCTGCCTCGAGGGTTACAGCCGTAATGCCATATCTCTGCTACTCGAGACAGCCA GATATTCCATATACTGCCAAGGGTGCTCCCATAATTTCCAAGCCTAAAGAAAACTATACT TTTGAATCGCATCCAGGCACACCCGTGTCATCTTCTTTAATGACGCAAAGACCAGGTGCT GAGAGCTCGTTGAAGAGTTTGGATAGTGCAATACGATCAACTATCAACTTAGAAAATCCT CAACCTATCAGAACACCAAACAGCAGTGCTACGGCGAATAACAATTTCGACATCAAGAAG ACGCTTTCTTTTTCAAGAATTCCTATGATTCCCGGTGGTAAGTTACAAAATACAAGCAAT AGCACGGACGCTGGTGAATTGTTCAACGCTCAAAATGCAGGCTATAAGCTATGGGTAGTA CAAGCCGGTACTTTGATTGCTCATTTGTTGAGTGCTGCAGGTGCTGACCATGTGATCACA ATGGATTTGCACGATCCACAGTTCCCTGGGTTTTTTGACATTCCAGTGGATAATCTCTAC TGTAAACCCATTGCACAAAACTACATCCAGCATCGCATTCCAGATTATCAGGATGCTGTG ATCGTTTCTCCAGATGCTGGTGGTGCAAAGAGAGCTACGGCTATTGCAGACGCCTTGGAA TTGTCCTTCGCCCTAATTCATAAAGAAAGAAGATCTCAGTTATTGAAGGGCCCTCCAGAT GCGACGTTAACCTCTGGTGGTTCGTTACCAGTATCTCCAAGGCCATTAGTTACTACTTTG GTTTCCTCCCAAAATACTACTTCTTCAGGTGCCACTGGGGTTGCGGCCCTTGAAATGAAG AAAACAACTTCAACATCTTCCACCTCGTCGCAATCTTCTAATTCGTCCAAGTTCGTTCAA ACTACCATGCTTGTTGGCGATGTTAGAAACAAGGTGTGTATTATAGTCGACGACTTGGTG GATACTTCATACACTATTACAAGAGCTGCGAAATTGTTGAAGGATCAAGGATCTACCAAA GTTTATGCCTTAATAACGCACGGTGTTTTTTCCGGTGATGCGCTAGAAAGAATCGGCCAA AGTAGTATAGATAAGTTGATCATTTCTAACACGGTTCCTCAAGATAGAACACTACAGTAC CTAGGTAAGGACAGAGTGGATGTTATTGATGTCTCCTGCATAATCGGTGAAGCAATTAGA AGAATCCATAACGGTGAATCCATTTCTATGTTGTTCGAGCATGGATGGTAG

Protein Properties

Pfam Domain Function

Pribosyltran (PF00156 )

Protein Residues

496

Protein Molecular Weight

53504.19922

Protein Theoretical pI

7.31

Signalling Regions

None

Transmembrane Regions

None

Protein Sequence

>Ribose-phosphate pyrophosphokinase 5 MSMSNIVVFGGDSHPELVTKICENLDIHPSKVELGKFSNGETNIALRESVREKDVYIIQS GCGQVNDTFMQLLILISACKSASASRVTAVMPYLCYSRQPDIPYTAKGAPIISKPKENYT FESHPGTPVSSSLMTQRPGAESSLKSLDSAIRSTINLENPQPIRTPNSSATANNNFDIKK TLSFSRIPMIPGGKLQNTSNSTDAGELFNAQNAGYKLWVVQAGTLIAHLLSAAGADHVIT MDLHDPQFPGFFDIPVDNLYCKPIAQNYIQHRIPDYQDAVIVSPDAGGAKRATAIADALE LSFALIHKERRSQLLKGPPDATLTSGGSLPVSPRPLVTTLVSSQNTTSSGATGVAALEMK KTTSTSSTSSQSSNSSKFVQTTMLVGDVRNKVCIIVDDLVDTSYTITRAAKLLKDQGSTK VYALITHGVFSGDALERIGQSSIDKLIISNTVPQDRTLQYLGKDRVDVIDVSCIIGEAIR RIHNGESISMLFEHGW

References

External Links

Resource	Link
Saccharomyces Genome Database	PRS5
Uniprot ID	Q12265
Uniprot Name	KPR5_YEAST
GenBank Gene ID	X91067
Genebank Protein ID	984179

General Reference

Mannhaupt, G., Vetter, I., Schwarzlose, C., Mitzel, S., Feldmann, H. (1996). "Analysis of a 26 kb region on the left arm of yeast chromosome XV." Yeast 12:67-76.8789261
Dujon, B., Albermann, K., Aldea, M., Alexandraki, D., Ansorge, W., Arino, J., Benes, V., Bohn, C., Bolotin-Fukuhara, M., Bordonne, R., Boyer, J., Camasses, A., Casamayor, A., Casas, C., Cheret, G., Cziepluch, C., Daignan-Fornier, B., Dang, D. V., de Haan, M., Delius, H., Durand, P., Fairhead, C., Feldmann, H., Gaillon, L., Kleine, K., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Nature 387:98-102.9169874
Hernando, Y., Parr, A., Schweizer, M. (1998). "PRS5, the fifth member of the phosphoribosyl pyrophosphate synthetase gene family in Saccharomyces cerevisiae, is essential for cell viability in the absence of either PRS1 or PRS3." J Bacteriol 180:6404-6407.9829955
Hernando, Y., Carter, A. T., Parr, A., Hove-Jensen, B., Schweizer, M. (1999). "Genetic analysis and enzyme activity suggest the existence of more than one minimal functional unit capable of synthesizing phosphoribosyl pyrophosphate in Saccharomyces cerevisiae." J Biol Chem 274:12480-12487.10212224
Ficarro, S. B., McCleland, M. L., Stukenberg, P. T., Burke, D. J., Ross, M. M., Shabanowitz, J., Hunt, D. F., White, F. M. (2002). "Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Nat Biotechnol 20:301-305.11875433
Huh, W. K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., O'Shea, E. K. (2003). "Global analysis of protein localization in budding yeast." Nature 425:686-691.14562095
Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
Hove-Jensen, B. (2004). "Heterooligomeric phosphoribosyl diphosphate synthase of Saccharomyces cerevisiae: combinatorial expression of the five PRS genes in Escherichia coli." J Biol Chem 279:40345-40350.15280369
Gruhler, A., Olsen, J. V., Mohammed, S., Mortensen, P., Faergeman, N. J., Mann, M., Jensen, O. N. (2005). "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Mol Cell Proteomics 4:310-327.15665377
Li, X., Gerber, S. A., Rudner, A. D., Beausoleil, S. A., Haas, W., Villen, J., Elias, J. E., Gygi, S. P. (2007). "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." J Proteome Res 6:1190-1197.17330950
Chi, A., Huttenhower, C., Geer, L. Y., Coon, J. J., Syka, J. E., Bai, D. L., Shabanowitz, J., Burke, D. J., Troyanskaya, O. G., Hunt, D. F. (2007). "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Proc Natl Acad Sci U S A 104:2193-2198.17287358
Smolka, M. B., Albuquerque, C. P., Chen, S. H., Zhou, H. (2007). "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Proc Natl Acad Sci U S A 104:10364-10369.17563356
Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956