Tryptophanyl-tRNA synthetase, cytoplasmic (Q12109)

Identification
NameTryptophanyl-tRNA synthetase, cytoplasmic
Synonyms
  • Tryptophan--tRNA ligase
  • TrpRS
Gene NameWRS1
Enzyme Class
Biological Properties
General FunctionInvolved in nucleotide binding
Specific FunctionATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp)
Cellular LocationCytoplasm
SMPDB Pathways
Tryptophan metabolismPW002442 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
Tryptophan metabolismec00380 Map00380
SMPDB ReactionsNot Available
KEGG Reactions
Adenosine triphosphate + tRNA(Trp) + L-Tryptophan → Adenosine monophosphate + Pyrophosphate + Trp-tRNA(Trp)
Metabolites
YMDB IDNameView
YMDB00097Adenosine monophosphateShow
YMDB00109Adenosine triphosphateShow
YMDB00126L-TryptophanShow
YMDB00219PyrophosphateShow
GO Classification
Component
intracellular part
cytoplasm
cell part
Function
binding
nucleoside binding
purine nucleoside binding
adenyl nucleotide binding
tryptophan-tRNA ligase activity
adenyl ribonucleotide binding
ATP binding
ligase activity, forming carbon-oxygen bonds
ligase activity, forming aminoacyl-tRNA and related compounds
catalytic activity
aminoacyl-tRNA ligase activity
nucleotide binding
ligase activity
Process
biosynthetic process
tRNA metabolic process
tRNA aminoacylation
tRNA aminoacylation for protein translation
macromolecule biosynthetic process
cellular macromolecule biosynthetic process
translation
tryptophanyl-tRNA aminoacylation
metabolic process
macromolecule metabolic process
cellular macromolecule metabolic process
RNA metabolic process
ncRNA metabolic process
Gene Properties
Chromosome Locationchromosome 15
LocusYOL097C
Gene Sequence>1299 bp ATGAGCAACGACGAAACTGTAGAGAAAGTCACCCAACAAGTGTCGGAACTAAAAAGCACA GATGTTAAAGAGCAAGTAGTTACACCTTGGGATGTGGAAGGTGGGGTTGATGAACAAGGT AGAGCCCAAAATATTGATTACGACAAATTGATCAAACAATTCGGTACTAAGCCGGTCAAC GAAGAAACCCTGAAGAGATTCAAGCAAGTGACGGGTCGTGAACCACATCATTTTTTGCGT AAGGGATTGTTTTTCAGTGAGCGTGACTTCACTAAAATATTAGACCTTTACGAACAAGGC AAACCATTTTTCCTATACACTGGTAGAGGTCCTTCGAGCGATTCTATGCACTTGGGTCAT ATGATCCCTTTTGTCTTCACCAAATGGTTACAGGAAGTGTTTGACGTACCATTAGTCATA GAGTTGACAGATGACGAAAAATTTTTATTCAAACACAAGTTGACCATCAATGACGTTAAG AATTTTGCCCGTGAAAATGCCAAGGATATCATTGCTGTTGGCTTTGATCCAAAGAACACC TTTATCTTTTCTGATTTGCAATACATGGGTGGTGCATTTTACGAAACTGTAGTAAGAGTT TCCAGACAAATTACAGGATCCACTGCAAAGGCTGTTTTCGGGTTTAATGACTCCGACTGT ATTGGTAAGTTCCATTTTGCCTCCATTCAAATTGCTACCGCATTCCCAAGCTCATTTCCT AATGTGTTAGGCTTGCCTGATAAGACACCATGTTTGATTCCATGTGCAATTGACCAAGAT CCATATTTCAGAGTTTGTAGGGATGTCGCGGATAAATTGAAGTACTCCAAACCTGCTTTG CTTCATTCCAGATTCTTTCCAGCTTTGCAAGGTTCCACGACCAAAATGTCAGCCTCTGAT GATACCACTGCCATTTTCATGACCGATACACCAAAGCAAATTCAAAAGAAAATTAACAAG TACGCATTCAGCGGTGGTCAAGTGTCCGCCGACCTACATAGAGAATTAGGTGGTAATCCC GATGTCGATGTTGCATACCAATACTTGTCATTTTTCAAGGATGACGATGTTTTCTTGAAG GAATGCTATGACAAATATAAGTCCGGTGAATTACTATCAGGTGAAATGAAGAAACTGTGT ATCGAGACTCTGCAAGAATTCGTTAAGGCGTTCCAGGAACGCAGAGCTCAGGTGGACGAA GAGACCTTGGACAAATTCATGGTCCCACATAAGTTGGTTTGGGGCGAAAAGGAAAGACTT GTCGCACCTAAGCCAAAAACTAAGCAAGAAAAGAAGTAA
Protein Properties
Pfam Domain Function
Protein Residues432
Protein Molecular Weight49350.0
Protein Theoretical pI6.79
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Tryptophanyl-tRNA synthetase, cytoplasmic MSNDETVEKVTQQVSELKSTDVKEQVVTPWDVEGGVDEQGRAQNIDYDKLIKQFGTKPVN EETLKRFKQVTGREPHHFLRKGLFFSERDFTKILDLYEQGKPFFLYTGRGPSSDSMHLGH MIPFVFTKWLQEVFDVPLVIELTDDEKFLFKHKLTINDVKNFARENAKDIIAVGFDPKNT FIFSDLQYMGGAFYETVVRVSRQITGSTAKAVFGFNDSDCIGKFHFASIQIATAFPSSFP NVLGLPDKTPCLIPCAIDQDPYFRVCRDVADKLKYSKPALLHSRFFPALQGSTTKMSASD DTTAIFMTDTPKQIQKKINKYAFSGGQVSADLHRELGGNPDVDVAYQYLSFFKDDDVFLK ECYDKYKSGELLSGEMKKLCIETLQEFVKAFQERRAQVDEETLDKFMVPHKLVWGEKERL VAPKPKTKQEKK
References
External Links
ResourceLink
Saccharomyces Genome Database WRS1
Uniprot IDQ12109
Uniprot NameSYWC_YEAST
GenBank Gene IDZ48149
Genebank Protein ID663256
General Reference
  • Vandenbol, M., Durand, P., Portetelle, D., Hilger, F. (1995). "Sequence analysis of a 44 kb DNA fragment of yeast chromosome XV including the Tyl-H3 retrotransposon, the suf1(+) frameshift suppressor gene for tRNA-Gly, the yeast transfer RNA-Thr-1a and a delta element." Yeast 11:1069-1075.7502582
  • Dujon, B., Albermann, K., Aldea, M., Alexandraki, D., Ansorge, W., Arino, J., Benes, V., Bohn, C., Bolotin-Fukuhara, M., Bordonne, R., Boyer, J., Camasses, A., Casamayor, A., Casas, C., Cheret, G., Cziepluch, C., Daignan-Fornier, B., Dang, D. V., de Haan, M., Delius, H., Durand, P., Fairhead, C., Feldmann, H., Gaillon, L., Kleine, K., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Nature 387:98-102.9169874
  • John, T. R., Ghosh, M., Johnson, J. D. (1997). "Identification and expression of the Saccharomyces cerevisiae cytoplasmic tryptophanyl-tRNA synthetase gene." Yeast 13:37-41.9046085
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956