Mitochondrial 2-methylisocitrate lyase (Q12031)

Identification
NameMitochondrial 2-methylisocitrate lyase
Synonyms
  • Methylisocitrate lyase
Gene NameICL2
Enzyme Class
Biological Properties
General FunctionInvolved in isocitrate lyase activity
Specific FunctionCatalyzes the formation of pyruvate and succinate from 2-methylisocitrate during the metabolism of endogenous propionyl- CoA. Does not act on isocitrate
Cellular LocationMitochondrion matrix
SMPDB PathwaysNot Available
KEGG Pathways
Propanoate metabolismec00640 Map00640
SMPDB ReactionsNot Available
KEGG Reactions
Methylisocitric acid → Pyruvic acid + Succinic acid
Metabolites
YMDB IDNameView
YMDB00094Methylisocitric acidShow
YMDB00175Pyruvic acidShow
YMDB00338Succinic acidShow
YMDB00535(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylic acidShow
GO Classification
Component
Not Available
Function
catalytic activity
oxo-acid-lyase activity
lyase activity
carbon-carbon lyase activity
isocitrate lyase activity
Process
oxoacid metabolic process
carboxylic acid metabolic process
metabolic process
cellular metabolic process
organic acid metabolic process
Gene Properties
Chromosome Locationchromosome 16
LocusYPR006C
Gene Sequence>1728 bp ATGATTACAATGATTAACAACAAAACTTTCAATAGAAAAACTACGGGAACACTGAAGAAG CTGGTTTTGTCATCAGACAAGTCACTGCGAAGATCTTTCAACGGGGCAAGCAGTACTAAA GACTTCGTGTTCTCGGAATCAAGTAAAGTAGAAGAATGGTGGGAATCAGCCAGATTTAAG AATATCAGTCGTCCATACTCAGCTACGGATGTTGTAAAGCATCGGGGCAGCCTTCCTGCT AATACTTCAATTTATCCTTCATCTTATCAAGCTCGAAAGCTGTTCAACTTACTAGAAGAG AATTTCAAGAATGGTACTCCACTTCACACGTTGGGAGTTATCGATCCTGTACAAATGAGT CAGTTGGCCCGTTGCAGAAATATCAAAGTTGCTTACATATCTGGTTGGGCTTGTTCTTCC ACATTAGTTGGATCTACAAATGAAGTATCGCCGGATTTTGGCGATTATCCATACGATACT GTGCCGAATCAAGTGGAGAGAATATTCAAGGCGCAGCAATTGCACGATCGAAAGGCTTTC TTGGAGGCCTCCATCAAAGGTTCTACTCCTGTTGATTATTTAAAACCAATTATCGCGGAC GCAGACATGGGTCACGGTGGCCCAACGACTGTAATGAAAGTTGCTAAGTTGTTTGCGGAG AAAGGGGCTGCTGGTATTCATCTTGAAGATCAGATGGTGGGAGGCAAAAGGTGTGGCCAT CTAAGTGGGGCTGTTTTGGTGCCCACAGCAACACATCTTATGAGATTGATTTCCACCAGG TTTCAATGGGATATCATGGGGACAGAAAATTTGGTTATTGCAAGAACAGATTCCTGTAAT GGTAAGCTGTTGAGTTCAAGCAGTGATCCGAGAGACCACGAATTCATTAGGGGTATAATA AGAGATAACGTGGTGCCTTGGAGCGAAAAATTGATTGAGATGGAGGACAAAAAAATTCCC AACTCCGCCATTGCTGACATGGAAAAAGAATGGTACCATGAAAATGAGCTGTTTACATTT GAAGAGGCCTTAGAAAAGCAGTTTACGGCAAGCGAGTTTGAAAGCTATAAAGAAAAGAAG GAAGACCTTATGGTAAACAAGCTGGGTAGAGCATACCTAAGCTTAAGAGAAATGAAACTT TTAGCTCAAGAAGTGACACCACTAAAGAAAATTATATTTGACTGGGATGCTCCGAGGACC AAAGAGGGATATTACATGTTTAACGGTTGTATTGAAGCAGCTATTAGAAGATCTTTGGTG TTTGCCCCATATTCTGATATGATTTGGCTGGAAACGAAAACTCCTGACCTGGAGCAGGCA CGCTCATTTTCAAGAAAAATTCATAAACAACTTCCAGCTACCAAGCTAGTATATAACTTA TCTCCTAGTTTCAATTGGAGCGCTCACGGCTTTGATGATAAGGCATTAAAATCCTTCGTA TGGGACTTGGCAAAAGAAGGCTTCACGTTGCAACTGGTTTCCCTTGCCGGTCTGCATTCA GATGGAGTATCATTCTGGGAATTAGCCAATAGCTTCCAAAGTGACGGAATGAAAGCATAC GTCGAAAAAGTGCAAAAACGGGAAAAGGAGACTAATTGTGATATCATGACACACCAGCTA TGGTCTGGTGCAGAATATGTTGATTCCTTAATGAAAGTTGTCCAAAACGGTGCTTCATCC CAAACTTTAAGTACATCTGGTGAGAGCTTTACCGAAACGCAATTTTGA
Protein Properties
Pfam Domain Function
Protein Residues575
Protein Molecular Weight64975.39844
Protein Theoretical pI7.63
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Mitochondrial 2-methylisocitrate lyase MITMINNKTFNRKTTGTLKKLVLSSDKSLRRSFNGASSTKDFVFSESSKVEEWWESARFK NISRPYSATDVVKHRGSLPANTSIYPSSYQARKLFNLLEENFKNGTPLHTLGVIDPVQMS QLARCRNIKVAYISGWACSSTLVGSTNEVSPDFGDYPYDTVPNQVERIFKAQQLHDRKAF LEASIKGSTPVDYLKPIIADADMGHGGPTTVMKVAKLFAEKGAAGIHLEDQMVGGKRCGH LSGAVLVPTATHLMRLISTRFQWDIMGTENLVIARTDSCNGKLLSSSSDPRDHEFIRGII RDNVVPWSEKLIEMEDKKIPNSAIADMEKEWYHENELFTFEEALEKQFTASEFESYKEKK EDLMVNKLGRAYLSLREMKLLAQEVTPLKKIIFDWDAPRTKEGYYMFNGCIEAAIRRSLV FAPYSDMIWLETKTPDLEQARSFSRKIHKQLPATKLVYNLSPSFNWSAHGFDDKALKSFV WDLAKEGFTLQLVSLAGLHSDGVSFWELANSFQSDGMKAYVEKVQKREKETNCDIMTHQL WSGAEYVDSLMKVVQNGASSQTLSTSGESFTETQF
References
External Links
ResourceLink
Saccharomyces Genome Database ICL2
Uniprot IDQ12031
Uniprot NameACEB_YEAST
GenBank Gene IDZ48951
Genebank Protein ID763005
General Reference
  • Bussey, H., Storms, R. K., Ahmed, A., Albermann, K., Allen, E., Ansorge, W., Araujo, R., Aparicio, A., Barrell, B., Badcock, K., Benes, V., Botstein, D., Bowman, S., Bruckner, M., Carpenter, J., Cherry, J. M., Chung, E., Churcher, C., Coster, F., Davis, K., Davis, R. W., Dietrich, F. S., Delius, H., DiPaolo, T., Hani, J., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Nature 387:103-105.9169875
  • Heinisch, J. J., Valdes, E., Alvarez, J., Rodicio, R. (1996). "Molecular genetics of ICL2, encoding a non-functional isocitrate lyase in Saccharomyces cerevisiae." Yeast 12:1285-1295.8923733
  • Luttik, M. A., Kotter, P., Salomons, F. A., van der Klei, I. J., van Dijken, J. P., Pronk, J. T. (2000). "The Saccharomyces cerevisiae ICL2 gene encodes a mitochondrial 2-methylisocitrate lyase involved in propionyl-coenzyme A metabolism." J Bacteriol 182:7007-7013.11092862
  • Huh, W. K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., O'Shea, E. K. (2003). "Global analysis of protein localization in budding yeast." Nature 425:686-691.14562095
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Sickmann, A., Reinders, J., Wagner, Y., Joppich, C., Zahedi, R., Meyer, H. E., Schonfisch, B., Perschil, I., Chacinska, A., Guiard, B., Rehling, P., Pfanner, N., Meisinger, C. (2003). "The proteome of Saccharomyces cerevisiae mitochondria." Proc Natl Acad Sci U S A 100:13207-13212.14576278