DNA ligase 4 (Q08387)

Identification
NameDNA ligase 4
Synonyms
  • DNA ligase II
  • DNA ligase IV
  • Polydeoxyribonucleotide synthase [ATP] 4
Gene NameDNL4
Enzyme Class
Biological Properties
General FunctionInvolved in DNA ligase (ATP) activity
Specific FunctionHas minor DNA joining activity. Can act on oligo(PDT)/poly(rA) substrate
Cellular LocationNucleus (Potential)
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00097Adenosine monophosphateShow
GO Classification
Component
intracellular
cell part
Function
catalytic activity
DNA ligase (ATP) activity
DNA binding
ligase activity, forming phosphoric ester bonds
DNA ligase activity
ligase activity
binding
nucleoside binding
purine nucleoside binding
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
nucleic acid binding
Process
DNA replication
metabolic process
macromolecule metabolic process
DNA ligation
DNA ligation involved in DNA repair
DNA recombination
DNA repair
cellular macromolecule metabolic process
DNA metabolic process
Gene Properties
Chromosome Locationchromosome 15
LocusYOR005C
Gene Sequence>2835 bp ATGATATCAGCACTAGATTCTATACCCGAGCCCCAAAACTTTGCGCCTAGTCCAGATTTC AAATGGCTTTGTGAAGAGCTATTTGTGAAGATACATGAAGTTCAAATTAATGGAACGGCC GGCACTGGCAAATCAAGGTCTTTCAAGTACTATGAAATAATATCGAATTTCGTCGAAATG TGGAGAAAAACCGTGGGAAATAATATATATCCTGCACTGGTTCTTGCTCTTCCCTACCGC GATAGACGAATCTATAATATTAAGGATTATGTATTAATAAGAACTATATGCTCTTACTTG AAGTTGCCAAAAAATTCTGCAACAGAGCAGCGGTTAAAAGATTGGAAACAGCGTGTCGGT AAAGGTGGGAATCTTTCTTCTCTTCTTGTGGAAGAAATTGCTAAAAGAAGGGCTGAACCT AGCTCAAAAGCGATTACAATTGATAACGTCAATCACTATCTGGATAGTTTGAGTGGAGAC AGGTTCGCTTCCGGACGAGGATTTAAGAGTCTTGTCAAGTCCAAACCTTTCCTGCACTGT GTGGAGAATATGAGTTTCGTCGAATTAAAATACTTCTTTGATATCGTGCTTAAAAATAGA GTAATAGGAGGTCAAGAGCACAAATTGCTAAACTGCTGGCATCCTGATGCTCAGGATTAT CTTAGCGTGATATCTGATTTAAAGGTGGTAACTTCAAAACTTTATGATCCAAAAGTTCGT CTAAAGGATGATGATTTGAGTATAAAAGTTGGCTTTGCATTCGCCCCCCAATTAGCCAAA AAAGTGAATCTTTCTTATGAGAAAATATGCCGTACACTACATGATGATTTTTTGGTAGAA GAAAAAATGGATGGAGAACGAATTCAAGTTCATTATATGAATTATGGTGAATCCATAAAA TTTTTTAGTAGACGGGGCATCGACTATACCTATTTGTACGGAGCGAGCTTATCATCAGGA ACTATATCTCAACATTTGAGGTTTACAGATAGTGTTAAAGAATGTGTTTTAGATGGAGAA ATGGTGACGTTTGATGCAAAAAGACGGGTGATTCTTCCATTCGGTCTTGTTAAAGGAAGT GCAAAGGAAGCGCTATCTTTTAATAGTATAAATAATGTTGACTTTCACCCCTTATATATG GTGTTTGATCTGTTATACCTGAATGGGACTTCGTTGACACCATTACCCCTTCATCAAAGG AAGCAATATCTGAACAGCATTTTAAGTCCCTTGAAAAATATTGTAGAAATAGTACGATCT TCTAGATGTTATGGTGTGGAGTCAATCAAAAAGTCTTTAGAAGTTGCAATCTCACTGGGT TCAGAAGGAGTTGTTTTGAAATATTATAATTCAAGTTATAATGTCGCCAGTCGAAACAAC AACTGGATCAAGGTAAAACCTGAATATTTGGAGGAATTTGGAGAGAATTTAGACTTAATA GTAATAGGCAGAGATTCTGGGAAAAAAGATTCTTTTATGCTAGGGTTACTTGTGCTAGAT GAAGAAGAGTATAAAAAGCACCAAGGAGACTCCTCTGAAATTGTAGACCACTCAAGCCAA GAAAAACACATACAAAATTCAAGAAGAAGGGTGAAAAAAATACTTTCATTCTGTTCTATC GCAAACGGTATATCTCAAGAAGAATTCAAAGAAATCGACCGCAAAACGAGAGGACATTGG AAAAGAACCTCCGAAGTTGCTCCCCCTGCTTCAATTTTAGAATTTGGCTCAAAAATACCT GCCGAATGGATTGACCCCAGTGAATCAATTGTTCTAGAAATAAAATCACGGTCTTTGGAT AACACAGAAACGAATATGCAGAAGTACGCTACCAATTGTACTTTGTACGGTGGCTATTGT AAAAGAATACGGTACGATAAAGAATGGACAGATTGTTACACACTTAACGACTTATACGAA AGTAGGACGGTTAAATCTAACCCCAGCTATCAAGCGGAAAGGTCACAGCTTGGATTGATA CGGAAAAAGAGAAAGAGAGTACTTATTTCAGACAGCTTTCACCAAAACAGGAAACAACTG CCAATTTCAAACATCTTTGCCGGATTACTTTTTTATGTTCTCTCTGACTATGTCACGGAG GACACTGGAATACGGATTACACGGGCAGAACTTGAAAAAACTATTGTGGAACATGGTGGT AAACTGATATATAATGTAATTTTAAAACGTCATTCAATTGGGGACGTTCGGTTAATCAGC TGTAAAACTACCACGGAATGCAAGGCTTTAATAGATCGAGGATATGATATATTGCACCCA AATTGGGTACTCGATTGTATAGCATATAAGAGGCTCATCCTGATCGAGCCCAATTATTGC TTTAACGTCTCTCAAAAAATGAGAGCCGTCGCTGAAAAAAGGGTAGATTGTTTGGGTGAT AGTTTTGAAAATGACATTTCGGAAACCAAACTGTCATCATTGTATAAATCACAACTAAGT CTACCACCGATGGGGGAACTCGAGATAGATTCTGAGGTTCGGCGGTTTCCATTATTTTTA TTCTCCAACAGGATTGCATACGTACCACGTCGCAAAATTAGCACAGAAGATGACATTATA GAAATGAAAATTAAGTTGTTTGGTGGAAAAATAACAGATCAACAGTCACTTTGTAACTTA ATAATTATACCATATACTGATCCTATTTTGAGGAAAGACTGCATGAATGAGGTACACGAA AAAATAAAAGAACAAATAAAGGCTTCTGATACTATACCGAAAATAGCCAGGGTCGTTGCC CCTGAATGGGTGGATCATTCTATTAATGAAAACTGTCAAGTGCCTGAAGAAGACTTCCCC GTAGTCAACTACTGA
Protein Properties
Pfam Domain Function
Protein Residues944
Protein Molecular Weight108514.0
Protein Theoretical pI8.55
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>DNA ligase 4 MISALDSIPEPQNFAPSPDFKWLCEELFVKIHEVQINGTAGTGKSRSFKYYEIISNFVEM WRKTVGNNIYPALVLALPYRDRRIYNIKDYVLIRTICSYLKLPKNSATEQRLKDWKQRVG KGGNLSSLLVEEIAKRRAEPSSKAITIDNVNHYLDSLSGDRFASGRGFKSLVKSKPFLHC VENMSFVELKYFFDIVLKNRVIGGQEHKLLNCWHPDAQDYLSVISDLKVVTSKLYDPKVR LKDDDLSIKVGFAFAPQLAKKVNLSYEKICRTLHDDFLVEEKMDGERIQVHYMNYGESIK FFSRRGIDYTYLYGASLSSGTISQHLRFTDSVKECVLDGEMVTFDAKRRVILPFGLVKGS AKEALSFNSINNVDFHPLYMVFDLLYLNGTSLTPLPLHQRKQYLNSILSPLKNIVEIVRS SRCYGVESIKKSLEVAISLGSEGVVLKYYNSSYNVASRNNNWIKVKPEYLEEFGENLDLI VIGRDSGKKDSFMLGLLVLDEEEYKKHQGDSSEIVDHSSQEKHIQNSRRRVKKILSFCSI ANGISQEEFKEIDRKTRGHWKRTSEVAPPASILEFGSKIPAEWIDPSESIVLEIKSRSLD NTETNMQKYATNCTLYGGYCKRIRYDKEWTDCYTLNDLYESRTVKSNPSYQAERSQLGLI RKKRKRVLISDSFHQNRKQLPISNIFAGLLFYVLSDYVTEDTGIRITRAELEKTIVEHGG KLIYNVILKRHSIGDVRLISCKTTTECKALIDRGYDILHPNWVLDCIAYKRLILIEPNYC FNVSQKMRAVAEKRVDCLGDSFENDISETKLSSLYKSQLSLPPMGELEIDSEVRRFPLFL FSNRIAYVPRRKISTEDDIIEMKIKLFGGKITDQQSLCNLIIIPYTDPILRKDCMNEVHE KIKEQIKASDTIPKIARVVAPEWVDHSINENCQVPEEDFPVVNY
References
External Links
ResourceLink
Saccharomyces Genome Database DNL4
Uniprot IDQ08387
Uniprot NameDNLI4_YEAST
GenBank Gene IDZ74913
Genebank Protein ID1420096
General Reference
  • Sterky, F., Holmberg, A., Pettersson, B., Uhlen, M. (1996). "The sequence of a 30 kb fragment on the left arm of chromosome XV from Saccharomyces cerevisiae reveals 15 open reading frames, five of which correspond to previously identified genes." Yeast 12:1091-1095.8896276
  • Dujon, B., Albermann, K., Aldea, M., Alexandraki, D., Ansorge, W., Arino, J., Benes, V., Bohn, C., Bolotin-Fukuhara, M., Bordonne, R., Boyer, J., Camasses, A., Casamayor, A., Casas, C., Cheret, G., Cziepluch, C., Daignan-Fornier, B., Dang, D. V., de Haan, M., Delius, H., Durand, P., Fairhead, C., Feldmann, H., Gaillon, L., Kleine, K., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Nature 387:98-102.9169874
  • Ramos, W., Tappe, N., Talamantez, J., Friedberg, E. C., Tomkinson, A. E. (1997). "Two distinct DNA ligase activities in mitotic extracts of the yeast Saccharomyces cerevisiae." Nucleic Acids Res 25:1485-1492.9092653
  • Herrmann, G., Lindahl, T., Schar, P. (1998). "Saccharomyces cerevisiae LIF1: a function involved in DNA double-strand break repair related to mammalian XRCC4." EMBO J 17:4188-4198.9670033
  • Tseng, H. M., Tomkinson, A. E. (2002). "A physical and functional interaction between yeast Pol4 and Dnl4-Lif1 links DNA synthesis and ligation in nonhomologous end joining." J Biol Chem 277:45630-45637.12235149
  • Dore, A. S., Furnham, N., Davies, O. R., Sibanda, B. L., Chirgadze, D. Y., Jackson, S. P., Pellegrini, L., Blundell, T. L. (2006). "Structure of an Xrcc4-DNA ligase IV yeast ortholog complex reveals a novel BRCT interaction mode." DNA Repair (Amst) 5:362-368.16388993