Diacylglycerol pyrophosphate phosphatase 1 (Q05521)

Identification
NameDiacylglycerol pyrophosphate phosphatase 1
Synonyms
  • DGPP phosphatase
  • Phosphatidate phosphatase
Gene NameDPP1
Enzyme Class
Biological Properties
General FunctionInvolved in catalytic activity
Specific FunctionCatalyzes the dephosphorylation of diacylglycerol phosphate (DGPP) to phosphatidate (PA) and the subsequent dephosphorylation of PA to diacylglycerol (DAG). Together with LPP1, regulates intracellular DGPP and PA levels which are phospholipid molecules believed to play a signaling role in stress response. Can also use lysophosphatidic acid (LPA) as a substrate. Substrate preference is DGPP > LPA > PA
Cellular LocationVacuole membrane; Multi-pass membrane protein
SMPDB Pathways
Ether lipid metabolismPW002492 ThumbThumb?image type=greyscaleThumb?image type=simple
Glycerophospholipid metabolismPW002493 ThumbThumb?image type=greyscaleThumb?image type=simple
Sphingolipid metabolismPW002479 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
Ether lipid metabolismec00565 Map00565
Glycerolipid metabolismec00561 Map00561
Glycerophospholipid metabolismec00564 Map00564
Sphingolipid metabolismec00600 Map00600
SMPDB ReactionsNot Available
KEGG Reactions
LPA(16:0/0:0) + water ↔ DG(16:0/16:0/0:0) + phosphate + hydron
Metabolites
YMDB IDNameView
YMDB00818lysophosphatidic acidShow
YMDB00862hydronShow
YMDB00890waterShow
YMDB00907phosphateShow
YMDB01170PA(18:1(9Z)/0:0)Show
GO Classification
Component
cell part
membrane
Function
catalytic activity
Process
Not Available
Gene Properties
Chromosome Locationchromosome 4
LocusYDR284C
Gene Sequence>870 bp ATGAACAGAGTTTCGTTTATTAAAACGCCTTTCAACATAGGGGCGAAATGGAGATTAGAA GATGTCTTTTTGCTCATTATCATGATACTTCTTAACTACCCAGTGTATTACCAACAACCG TTCGAACGTCAGTTTTACATTAACGATCTCACTATATCGCATCCTTATGCGACAACTGAA CGTGTAAATAACAACATGTTGTTTGTTTATAGTTTTGTCGTGCCATCTTTAACCATATTG ATAATTGGTTCCATTTTGGCCGATAGAAGACACTTGATTTTTATTTTGTACACATCTCTC CTTGGTTTATCACTCGCTTGGTTCAGTACGAGTTTCTTTACAAACTTCATCAAGAATTGG ATTGGAAGACTAAGACCAGATTTTCTAGATCGTTGCCAACCTGTTGAAGGCTTGCCATTG GACACTTTATTTACTGCAAAAGATGTGTGTACGACTAAGAATCACGAACGTCTGTTGGAT GGGTTTAGGACAACTCCGTCAGGTCATTCAAGTGAAAGCTTTGCAGGACTGGGTTATTTG TACTTCTGGCTATGTGGGCAACTTTTGACTGAATCACCGTTGATGCCTTTATGGAGAAAA ATGGTGGCCTTTCTACCACTGTTAGGAGCTGCACTAATTGCTCTATCCAGAACTCAAGAT TACAGACATCATTTCGTCGATGTAATTTTAGGGTCTATGTTGGGTTATATAATGGCACAC TTTTTCTACAGAAGAATCTTCCCACCCATTGATGATCCTCTTCCGTTCAAACCATTGATG GACGATTCAGATGTCACCCTGGAGGAAGCAGTCACCCATCAGAGGATCCCGGATGAGGAA TTACATCCTTTGTCCGATGAAGGTATGTAA
Protein Properties
Pfam Domain Function
Protein Residues289
Protein Molecular Weight33513.60156
Protein Theoretical pI6.42
Signalling Regions
  • None
Transmembrane Regions
  • 22-42
  • 66-86
  • 93-113
  • 173-193
  • 194-214
  • 223-243
Protein Sequence>Diacylglycerol pyrophosphate phosphatase 1 MNRVSFIKTPFNIGAKWRLEDVFLLIIMILLNYPVYYQQPFERQFYINDLTISHPYATTE RVNNNMLFVYSFVVPSLTILIIGSILADRRHLIFILYTSLLGLSLAWFSTSFFTNFIKNW IGRLRPDFLDRCQPVEGLPLDTLFTAKDVCTTKNHERLLDGFRTTPSGHSSESFAGLGYL YFWLCGQLLTESPLMPLWRKMVAFLPLLGAALIALSRTQDYRHHFVDVILGSMLGYIMAH FFYRRIFPPIDDPLPFKPLMDDSDVTLEEAVTHQRIPDEELHPLSDEGM
References
External Links
ResourceLink
Saccharomyces Genome Database DPP1
Uniprot IDQ05521
Uniprot NameDPP1_YEAST
GenBank Gene IDAY557744
Genebank Protein ID45269379
General Reference
  • Jacq, C., Alt-Morbe, J., Andre, B., Arnold, W., Bahr, A., Ballesta, J. P., Bargues, M., Baron, L., Becker, A., Biteau, N., Blocker, H., Blugeon, C., Boskovic, J., Brandt, P., Bruckner, M., Buitrago, M. J., Coster, F., Delaveau, T., del Rey, F., Dujon, B., Eide, L. G., Garcia-Cantalejo, J. M., Goffeau, A., Gomez-Peris, A., Zaccaria, P., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Nature 387:75-78.9169867
  • Hu, Y., Rolfs, A., Bhullar, B., Murthy, T. V., Zhu, C., Berger, M. F., Camargo, A. A., Kelley, F., McCarron, S., Jepson, D., Richardson, A., Raphael, J., Moreira, D., Taycher, E., Zuo, D., Mohr, S., Kane, M. F., Williamson, J., Simpson, A., Bulyk, M. L., Harlow, E., Marsischky, G., Kolodner, R. D., LaBaer, J. (2007). "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Genome Res 17:536-543.17322287
  • Wu, W. I., Liu, Y., Riedel, B., Wissing, J. B., Fischl, A. S., Carman, G. M. (1996). "Purification and characterization of diacylglycerol pyrophosphate phosphatase from Saccharomyces cerevisiae." J Biol Chem 271:1868-1876.8567632
  • Han, G. S., Johnston, C. N., Chen, X., Athenstaedt, K., Daum, G., Carman, G. M. (2001). "Regulation of the Saccharomyces cerevisiae DPP1-encoded diacylglycerol pyrophosphate phosphatase by zinc." J Biol Chem 276:10126-10133.11139591
  • Oshiro, J., Han, G. S., Iwanyshyn, W. M., Conover, K., Carman, G. M. (2003). "Regulation of the yeast DPP1-encoded diacylglycerol pyrophosphate phosphatase by transcription factor Gis1p." J Biol Chem 278:31495-31503.12799368
  • Han, G. S., Johnston, C. N., Carman, G. M. (2004). "Vacuole membrane topography of the DPP1-encoded diacylglycerol pyrophosphate phosphatase catalytic site from Saccharomyces cerevisiae." J Biol Chem 279:5338-5345.14630917
  • Toke, D. A., McClintick, M. L., Carman, G. M. (1999). "Mutagenesis of the phosphatase sequence motif in diacylglycerol pyrophosphate phosphatase from Saccharomyces cerevisiae." Biochemistry 38:14606-14613.10545184
  • Huh, W. K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., O'Shea, E. K. (2003). "Global analysis of protein localization in budding yeast." Nature 425:686-691.14562095
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Oshiro, J., Han, G. S., Carman, G. M. (2003). "Diacylglycerol pyrophosphate phosphatase in Saccharomyces cerevisiae." Biochim Biophys Acta 1635:1-9.14642771
  • Gruhler, A., Olsen, J. V., Mohammed, S., Mortensen, P., Faergeman, N. J., Mann, M., Jensen, O. N. (2005). "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Mol Cell Proteomics 4:310-327.15665377
  • Li, X., Gerber, S. A., Rudner, A. D., Beausoleil, S. A., Haas, W., Villen, J., Elias, J. E., Gygi, S. P. (2007). "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." J Proteome Res 6:1190-1197.17330950
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956