Lysophospholipase NTE1 (Q04958) - Yeast Metabolome Database

Identification

Name

Lysophospholipase NTE1

Synonyms

Intracellular phospholipase B
Neuropathy target esterase homolog

Gene Name

NTE1

Enzyme Class

3.1.1.5

Biological Properties

General Function

Involved in metabolic process

Specific Function

Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis. Responsible for the rapid PC turnover in response to inositol, elevated temperatures, or when choline is present in the growth medium

Cellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein

SMPDB Pathways

Choline metabolism	PW002494
Glycerophospholipid metabolism	PW002493
Lysolipid incorporation into ER	PW002532
Lysolipid incorporation into ER PC(10:0/10:0)	PW002780
Lysolipid incorporation into ER PC(14:0/14:0)	PW002783

KEGG Pathways

Glycerophospholipid metabolism

ec00564

SMPDB Reactions

PC(16:0/16:0) + water → Glycerophosphocholine + hydron + Palmitic acid

PC(14:0/0:0) + water → Glycerophosphocholine + a carboxylate

PC(16:0/0:0) + water → Glycerophosphocholine + a carboxylate

LysoPC(10:0) + water → Glycerophosphocholine + a carboxylate

PC(16:1(9Z)/0:0) + water → Glycerophosphocholine + a carboxylate

KEGG Reactions

fatty acid + hydron + Glycerophosphocholine ↔ water + phosphatidylcholine

Metabolites

YMDB ID	Name	View
YMDB00069	Palmitic acid	Show
YMDB00309	Glycerophosphocholine	Show
YMDB00694	sn-Glycero-3-phosphocholine	Show
YMDB00862	hydron	Show
YMDB00890	water	Show
YMDB01175	PC(14:0/0:0)	Show
YMDB01179	PC(16:0/0:0)	Show
YMDB01180	PC(16:0/16:0)	Show
YMDB01184	PC(16:1(9Z)/0:0)	Show
YMDB01187	PC(18:0/0:0)	Show
YMDB01190	PC(18:1(9Z)/0:0)	Show
YMDB02213	LysoPC(20:4(5Z,8Z,11Z,14Z))	Show
YMDB16163	LysoPC(10:0)	Show

GO Classification

Component
cell part
membrane
organelle membrane
endoplasmic reticulum membrane
Function
lysophospholipase activity
catalytic activity
hydrolase activity
hydrolase activity, acting on ester bonds
carboxylesterase activity
Process
glycerophospholipid metabolic process
phosphatidylcholine metabolic process
metabolic process
primary metabolic process
lipid metabolic process
organophosphate metabolic process
phospholipid metabolic process

Gene Properties

Chromosome Location

chromosome 13

Locus

YML059C

Gene Sequence

>5040 bp ATGCGTTCAATGAATTGCACTACGAACAACACCAACAATACCGGCCAAAACACGAAAAAC AGTCTTGGCTCCAGCTTTAATTCCAGTAATTATACTTCTTATAGATTCCAGACATGTCTC ACCGACCAGATTATTTCTGAGGCGCAGACGTGGTCACTTTCATCCCTCTTCAACTTCTCG TGGGTTGTGTCCTACTTTGTTATGGGTGCCTCTCGTATGATCTTCAGGTACGGTTGGTAT CTGGCAACTTTATCGCTTTTAAGGATTCCGAAGTGGATCTTCTTCAAGTTACACCATGTT CAGTTCACTCTTTCCTTTTGGCTGATCCTGTTTGCCCTAGCCGTCATAGTCTTTGTGACT TACACCATCATGAAGGAGAGAATTCTATCCCAGTACAAGAGGCTGACCCCGGAGTTCTTG CCGCTGGAAAATACAGGTAAATCTGGCTCCAGCGCCAATATCAATGCAGCTTCCACTCAG TCAGCCAACGCGCCACCCGCCATAGGTTCCTCTACCACGGGTGCAAGCTCGATTATAGAC TCAAAAAAGCATTCATTGAAAGATGGCAACGAAAACGAAACCTTTCTTTCATCTTACCTT GACCAATTTCTCAGCGCCATCAAAATCTTTGGCTATCTGGAAAAACCAGTCTTCCATGAC CTGACCAAGAATATGAAAACTCAAAAAATGGATGAGGGTGAGATTTTACTGTTGGACAGT ACTATTGGGTTTGCCATCGTCGTTGAAGGCACCCTGCAGTTATATCATGAAGTAGATCAT TCAGATAAGGACCATGGCGATGAAACTGACCACAGTGACACGGATGGTCTTGACGACCAG GATAGAGATGAAGAAGACGAAGAAGAAGATGATGACATTGATAACTACGACACCAAATCT TGTTCTTCTAACCTCATCGATGAAGAAGATGAATCCGTTGGTTACATTCATCTGAAAAAT GGACTGGGAAATTTTCAACTGCTAAATACAGTCAAGCCAGGAAACCCGCTGACCTCCCTC GTGAGCATTTTAAACTTATTCACGCATTCCATGTCCTCGTATGGCAACAGTAACTTTCCC TCTGAGTTATCGTCACCTATAGACACAACGGTGTCGGTCAACAATATGTTCTGCTCATCA GAGCAGAACTTCTCCAACACAGATTCCATGACAAACTCCACCAACAGCTTTCCAACGTTC CCCTCTTCAATGCCTAAACTTGTCGCCCGTGCGGCTACAGACTGCACAATTGGTATTATC CCACCTCAATCGTTTGCCAAATTAACCGCAAAATACCCACGGTCTGCTTCCCATATCATA CAGATGGTTCTCACCAAGCTATACCATGTTACGTTCCAAACTGCTCACGACTACTTAGGT TTAACAAAGGAGATCATGGATATCGAAGTTTTACTAAATAAATCCATCGTTTATGAATTG CCTTATTATTTGAAAGAGGCCGTTATTCGTAAGTTTAAAACTGTAGATAAGTCGAGCGGA TCGGCTGATTTAGAACCGAAACCCAAAAATAGTAATGCTTCCTCTAAATTGAAGAAACCG CCCAAAGCGAAGCCATCTGATGGTATCATTCAATCGCTTAAGATTGCTAACGCCAACGCT AACACAAGTTCGAATTCCCTTTCACTGAAACCAGAATTCACCCACCATCCAAGTTCGAGA CATGTCGTACTGGGTTCAAGAGACCAATTTAATCCTGGTGATTTACTGTCAAATGTCCCA CTGTCTAGAACTATGGATATTCTGTCACCCAACCCAATCCATAATAACAACCGCAATAAA TCAAACGGTATTAACACTTCTACCTCTAACCAACATAAGCGATCTTCTAGGAGTAGCAGT AACAATGCTTCCGTACACTCTAAGAAGTTTTCTTCTTTGTCCCCAGAATTGCGCAACGCA CAACTTAGTACATCGCCCTTGTCCTTGGATAATACTTCTGTTCATGACCATATCCACCCA AGTCCCGTTCATTTGAAGGGACGGGTCTCTCCAAGACCTAATTTACTGCCCACAACATCC TTTTCTGCTGCCCAGGAAGAAACCGAGGACTCTGCTTTAAGAATGGCTTTAGTTGAGGCA ATGTTAACATACTTGGGTGTAAACAAAAGCAATATGTCCGTTTCATCTTCTTCGATAGCA AATATGTCTTCTTTAAACTCTCCTCAACTTAATGAAATGTATAGCCGCCGTCCAAGTAAC GCAAGTTTTCTGATGTCCCCTCACTGTACACCATCTGACATTTCCGTAGCTTCATCATTT GCCTCTCCTCAAACTCAACCGACAATGTTAAGAATCCTACCCAAAGAATACACTATCAGC AATAAAAGGCATAATAAAAGTAAAAGCCAGGACAAAAAAAAACCTAGAGCTTACAAAGAG GAGTTGACACCCAATTTGGATTTCGAAGATGTCAAGAAAGACTTTGCACAAGGCATTCAG TTGAAGTTCTTTAAAAAGGGCACCACAATTGTTGAGCAGAACGCTCGTGGTAAAGGCCTA TTCTATATCATTTCCGGGAAAGTTAATGTGACAACAAATTCGTCCTCGTCAGTAGTATCA TCAATGTCTAAACCAGAGCAGGTTTCAGCCCAAAGTTCACACAAGGGTGAAAATCCTCAT CATACTCAACATTTGTTATACTCTGTAGGATCTGGTGGTATTGTGGGCTACTTGTCCTCT CTAATTGGTTACAAATCATTTGTTAATATCGTTGCCAAGAGCGATGTCTATGTTGGATTT TTATCTAGTGCAACCTTAGAAAGATTGTTTGATAAATATTTCCTAATCTACTTACGGATT TCTGATTCATTAACCAAATTGCTATCTTCAAGGTTATTAAAGCTCGACCATGCTTTAGAA TGGGTTCACTTGAGGGCTTCTGAAACTCTATTTTCACAAGGTGACTCGGCTAACGGTATT TATGTTGTCCTGAATGGTAGACTAAGGCAGCTACAACAGCAGTCATTGTCAAACTCCAAT ACAAGTTCCGAAGAAGTTGAAACTCAGAACATTATTCTAGGAGAACTGGCCCAGGGGGAG AGTTTTGGTGAAGTTGAAGTGCTTACCGCTATGAATAGATATTCAACGATAGTGGCAGTG AGGGATTCTGAATTAGCGAGAATTCCGAGAACATTATTCGAACTATTAGCTCTTGAACAT CCATCTATCATGATTAGAGTTTCCAGATTAGTTGCTAAAAAAATTGTCGGAGATAGAACT GTTCCAGCATTAACAGGCGATCCATTATCTATAAAGGAAAACGACTTCACTTCTTTAATA CCTCCAACGAAAGCGTCTTACTCTAGCTCTTTAAGTCATAAACCCCAAAATATTACTTCT GGCACAATTACTTTTAGAACCATCACTATCTTACCAATTACCAGTGGACTACCTGTAGAA GCATTTGCTATGAAGTTAGTGCAAGCTTTCAAACAGGTAGGAAGGACCACGATTGGTTTA AATCAAAGAACTACCCTAACACATTTAGGTCGTCATGCATTTGACAGATTGTCCAAATTA AAACAAAGTGGTTACTTTGCTGAACTAGAAGAAATGTACCAGACTGTTGTTTATATATCA GATACACCAGTTAAATCTAATTGGACAAGAACCTGTATTGCGCAAGGTGATTGTATTCTC TTACTAGCAGATGCTAGATCTCCATCAGCCGAAATAGGAGAATATGAAAAGTTATTACTA AATTCAAAGACTACCGCAAGGACCGAGTTAATTCTTCTACACCCTGAAAGGTATGTGGAA CCAGGACTAACCCATAAGTGGTTACGCTATAGGCCATGGGTTCATTCGCATCATCACATT CAATTTAGTTTGACAGGAACAACCTTGATGAACGAAGGCAAAATGCACGTTTTGAATAAT GGTGCCTTGGCATTAATGGATAAGTTGATTCAAACAGAATTCAGTAGAAAAACTCAACAG AATATATCAAAACTACTTCCAGATTCTATCAAGAACACCGTAGAAAACTTTTCTTCAAGA TTCATGAAAAGTAAAAGACAATATTACACACCAGTTCATAGGCATAAAAATGATTTTCTA AGATTGGCTAGAATTCTTTCTGGACAAGCTATTGGACTTGTCCTCGGAGGAGGAGGTGCG AGAGGTATTAGTCATTTGGGTGTCATTCAAGCTATTGAAGAACAAGGTATTCCGGTCGAC GTTATTGGAGGAACATCGATTGGTTCCTTTGTTGGCGGTTTGTACGCGAAGGATTATGAT TTGGTGCCAATTTACGGCCGCGTGAAGAAGTTTGCTGGTCGAATTTCCTCTATATGGAGA ATGTTAACAGATTTAACCTGGCCGGTTACTTCTTATACTACTGGTCACGAATTTAATAGA GGTATTTGGAAAACTTTCGGGGATACAAGAATTGAAGACTTCTGGATCCAGTATTATTGT AATTCTACTAATATAACCGATTCGGTTCAAGAAATACATTCCTTTGGTTATGCGTGGAGG TACATCAGAGCTTCGATGTCTCTTGCTGGTCTTTTACCTCCTTTGGAAGAGAACGGGTCT ATGTTACTGGATGGTGGTTATGTTGATAATTTACCAGTAACTGAGATGAGAGCGCGTGGT TGCCAAACTATATTTGCAGTAGATGTAGGTTCTGCAGATGATAGAACGCCTATGGAATAT GGTGACTCCCTAAATGGGTTTTGGATCATTTTCAATAGATGGAATCCATTTTCTTCTCAT CCAAACATACCCAATATGGCAGAGATTCAAGTTAGATTGGGTTACGTCGCATCAGTAAAT GCTTTAGAGAAAGCGAAAAATACACCAGGTGTCGTTTATGTGAGACCACCTATTGAAGAA TATGCCACATTAGACTTTAGCAAGTTCGAAGAAATTTATCATGTGGGTGTAGACTATGGT CGTATCTTTTTGCAAGGGCTTATTGACGACGACAAGATGCCATATATACCTGGTTCACAA GAAACCACTTTGAATTCTCAAGTTCCTGAGTTTTTATTACATAGAAGAAATAGTATTTGA

Protein Properties

Pfam Domain Function

cNMP_binding (PF00027 )
Patatin (PF01734 )

Protein Residues

1679

Protein Molecular Weight

187131.0

Protein Theoretical pI

8.24

Signalling Regions

None

Transmembrane Regions

50-70
104-124

Protein Sequence

>Lysophospholipase NTE1 MRSMNCTTNNTNNTGQNTKNSLGSSFNSSNYTSYRFQTCLTDQIISEAQTWSLSSLFNFS WVVSYFVMGASRMIFRYGWYLATLSLLRIPKWIFFKLHHVQFTLSFWLILFALAVIVFVT YTIMKERILSQYKRLTPEFLPLENTGKSGSSANINAASTQSANAPPAIGSSTTGASSIID SKKHSLKDGNENETFLSSYLDQFLSAIKIFGYLEKPVFHDLTKNMKTQKMDEGEILLLDS TIGFAIVVEGTLQLYHEVDHSDKDHGDETDHSDTDGLDDQDRDEEDEEEDDDIDNYDTKS CSSNLIDEEDESVGYIHLKNGLGNFQLLNTVKPGNPLTSLVSILNLFTHSMSSYGNSNFP SELSSPIDTTVSVNNMFCSSEQNFSNTDSMTNSTNSFPTFPSSMPKLVARAATDCTIGII PPQSFAKLTAKYPRSASHIIQMVLTKLYHVTFQTAHDYLGLTKEIMDIEVLLNKSIVYEL PYYLKEAVIRKFKTVDKSSGSADLEPKPKNSNASSKLKKPPKAKPSDGIIQSLKIANANA NTSSNSLSLKPEFTHHPSSRHVVLGSRDQFNPGDLLSNVPLSRTMDILSPNPIHNNNRNK SNGINTSTSNQHKRSSRSSSNNASVHSKKFSSLSPELRNAQLSTSPLSLDNTSVHDHIHP SPVHLKGRVSPRPNLLPTTSFSAAQEETEDSALRMALVEAMLTYLGVNKSNMSVSSSSIA NMSSLNSPQLNEMYSRRPSNASFLMSPHCTPSDISVASSFASPQTQPTMLRILPKEYTIS NKRHNKSKSQDKKKPRAYKEELTPNLDFEDVKKDFAQGIQLKFFKKGTTIVEQNARGKGL FYIISGKVNVTTNSSSSVVSSMSKPEQVSAQSSHKGENPHHTQHLLYSVGSGGIVGYLSS LIGYKSFVNIVAKSDVYVGFLSSATLERLFDKYFLIYLRISDSLTKLLSSRLLKLDHALE WVHLRASETLFSQGDSANGIYVVLNGRLRQLQQQSLSNSNTSSEEVETQNIILGELAQGE SFGEVEVLTAMNRYSTIVAVRDSELARIPRTLFELLALEHPSIMIRVSRLVAKKIVGDRT VPALTGDPLSIKENDFTSLIPPTKASYSSSLSHKPQNITSGTITFRTITILPITSGLPVE AFAMKLVQAFKQVGRTTIGLNQRTTLTHLGRHAFDRLSKLKQSGYFAELEEMYQTVVYIS DTPVKSNWTRTCIAQGDCILLLADARSPSAEIGEYEKLLLNSKTTARTELILLHPERYVE PGLTHKWLRYRPWVHSHHHIQFSLTGTTLMNEGKMHVLNNGALALMDKLIQTEFSRKTQQ NISKLLPDSIKNTVENFSSRFMKSKRQYYTPVHRHKNDFLRLARILSGQAIGLVLGGGGA RGISHLGVIQAIEEQGIPVDVIGGTSIGSFVGGLYAKDYDLVPIYGRVKKFAGRISSIWR MLTDLTWPVTSYTTGHEFNRGIWKTFGDTRIEDFWIQYYCNSTNITDSVQEIHSFGYAWR YIRASMSLAGLLPPLEENGSMLLDGGYVDNLPVTEMRARGCQTIFAVDVGSADDRTPMEY GDSLNGFWIIFNRWNPFSSHPNIPNMAEIQVRLGYVASVNALEKAKNTPGVVYVRPPIEE YATLDFSKFEEIYHVGVDYGRIFLQGLIDDDKMPYIPGSQETTLNSQVPEFLLHRRNSI

References

External Links

Resource	Link
Saccharomyces Genome Database	NTE1
Uniprot ID	Q04958
Uniprot Name	NTE1_YEAST
GenBank Gene ID	Z46729
Genebank Protein ID	577137

General Reference

Bowman, S., Churcher, C., Badcock, K., Brown, D., Chillingworth, T., Connor, R., Dedman, K., Devlin, K., Gentles, S., Hamlin, N., Hunt, S., Jagels, K., Lye, G., Moule, S., Odell, C., Pearson, D., Rajandream, M., Rice, P., Skelton, J., Walsh, S., Whitehead, S., Barrell, B. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Nature 387:90-93.9169872
Huh, W. K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., O'Shea, E. K. (2003). "Global analysis of protein localization in budding yeast." Nature 425:686-691.14562095
Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
Zaccheo, O., Dinsdale, D., Meacock, P. A., Glynn, P. (2004). "Neuropathy target esterase and its yeast homologue degrade phosphatidylcholine to glycerophosphocholine in living cells." J Biol Chem 279:24024-24033.15044461
Murray, J. P., McMaster, C. R. (2005). "Nte1p-mediated deacylation of phosphatidylcholine functionally interacts with Sec14p." J Biol Chem 280:8544-8552.15611060
Gruhler, A., Olsen, J. V., Mohammed, S., Mortensen, P., Faergeman, N. J., Mann, M., Jensen, O. N. (2005). "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Mol Cell Proteomics 4:310-327.15665377
Gaspar, M. L., Aregullin, M. A., Jesch, S. A., Henry, S. A. (2006). "Inositol induces a profound alteration in the pattern and rate of synthesis and turnover of membrane lipids in Saccharomyces cerevisiae." J Biol Chem 281:22773-22785.16777854
Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258
Li, X., Gerber, S. A., Rudner, A. D., Beausoleil, S. A., Haas, W., Villen, J., Elias, J. E., Gygi, S. P. (2007). "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." J Proteome Res 6:1190-1197.17330950
Smolka, M. B., Albuquerque, C. P., Chen, S. H., Zhou, H. (2007). "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Proc Natl Acad Sci U S A 104:10364-10369.17563356
Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956