Lipid phosphate phosphatase 1 (Q04396)

Identification
NameLipid phosphate phosphatase 1
Synonyms
  • Phosphatidate phosphatase
Gene NameLPP1
Enzyme Class
Biological Properties
General FunctionInvolved in catalytic activity
Specific FunctionCatalyzes the dephosphorylation of diacylglycerol phosphate (DGPP) to phosphatidate (PA) and the subsequent dephosphorylation of PA to diacylglycerol (DAG). Together with DPP1, regulates intracellular DGPP and PA levels which are phospholipid molecules believed to play a signaling role in stress response. Can also use lysophosphatidic acid (LPA) as a substrate. Substrate preference is PA > DGPP > LPA
Cellular LocationGolgi apparatus membrane; Multi-pass membrane protein
SMPDB Pathways
Ether lipid metabolismPW002492 ThumbThumb?image type=greyscaleThumb?image type=simple
Glycerophospholipid metabolismPW002493 ThumbThumb?image type=greyscaleThumb?image type=simple
Sphingolipid metabolismPW002479 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
Ether lipid metabolismec00565 Map00565
Glycerolipid metabolismec00561 Map00561
Glycerophospholipid metabolismec00564 Map00564
Sphingolipid metabolismec00600 Map00600
SMPDB ReactionsNot Available
KEGG Reactions
water + 1,2-diacylglycerol 3-diphosphate → LPA(16:0/0:0) + phosphate + hydron
Metabolites
YMDB IDNameView
YMDB00818lysophosphatidic acidShow
YMDB00862hydronShow
YMDB00890waterShow
YMDB00907phosphateShow
YMDB01170PA(18:1(9Z)/0:0)Show
GO Classification
Component
cell part
membrane
Function
catalytic activity
Process
Not Available
Gene Properties
Chromosome Locationchromosome 4
LocusYDR503C
Gene Sequence>825 bp ATGATCTCTGTCATGGCGGATGAGAAACATAAGGAGTATTTTAAGCTATACTACTTTCAG TACATGATAATTGGTCTATGTACGATATTATTCCTCTATTCGGAGATATCCCTGGTACCT AGGGGCCAAAACATCGAATTTAGTCTTGATGACCCCAGTATATCAAAACGTTATGTACCT AACGAACTCGTGGGCCCACTAGAATGTTTGATTTTGAGTGTTGGACTGAGTAACATGGTC GTCTTCTGGACCTGCATGTTTGACAAGGACTTACTGAAGAAGAATAGAGTAAAGAGACTA AGAGAGAGGCCGGACGGAATCTCGAACGATTTTCACTTCATGCATACTAGCATTCTATGT CTGATGCTGATTATAAGCATAAATGCTGCCCTAACAGGCGCCTTAAAGTTGATTATAGGA AACTTGAGGCCTGACTTTGTTGATAGATGTATACCTGACCTCCAAAAGATGAGTGATTCA GATTCTTTGGTTTTTGGCTTGGACATTTGCAAGCAGACTAACAAATGGATTCTATACGAA GGCTTAAAAAGCACTCCAAGCGGACATTCAAGTTTCATAGTCAGTACCATGGGCTTTACA TATCTTTGGCAAAGGGTTTTCACCACACGCAATACAAGAAGTTGCATTTGGTGCCCTTTA TTAGCTCTAGTAGTAATGGTTTCAAGGGTTATCGATCACAGACATCATTGGTACGATGTT GTCTCTGGAGCTGTTCTAGCATTTTTAGTCATTTATTGTTGCTGGAAATGGACATTTACA AACTTGGCGAAAAGAGACATACTTCCTTCACCGGTTAGTGTTTAG
Protein Properties
Pfam Domain Function
Protein Residues274
Protein Molecular Weight31585.90039
Protein Theoretical pI8.4
Signalling Regions
  • None
Transmembrane Regions
  • 16-33
  • 70-87
  • 118-139
  • 190-203
  • 215-231
  • 238-255
Protein Sequence>Lipid phosphate phosphatase 1 MISVMADEKHKEYFKLYYFQYMIIGLCTILFLYSEISLVPRGQNIEFSLDDPSISKRYVP NELVGPLECLILSVGLSNMVVFWTCMFDKDLLKKNRVKRLRERPDGISNDFHFMHTSILC LMLIISINAALTGALKLIIGNLRPDFVDRCIPDLQKMSDSDSLVFGLDICKQTNKWILYE GLKSTPSGHSSFIVSTMGFTYLWQRVFTTRNTRSCIWCPLLALVVMVSRVIDHRHHWYDV VSGAVLAFLVIYCCWKWTFTNLAKRDILPSPVSV
References
External Links
ResourceLink
Saccharomyces Genome Database LPP1
Uniprot IDQ04396
Uniprot NameLPP1_YEAST
GenBank Gene IDAY693191
Genebank Protein ID51013833
General Reference
  • Jacq, C., Alt-Morbe, J., Andre, B., Arnold, W., Bahr, A., Ballesta, J. P., Bargues, M., Baron, L., Becker, A., Biteau, N., Blocker, H., Blugeon, C., Boskovic, J., Brandt, P., Bruckner, M., Buitrago, M. J., Coster, F., Delaveau, T., del Rey, F., Dujon, B., Eide, L. G., Garcia-Cantalejo, J. M., Goffeau, A., Gomez-Peris, A., Zaccaria, P., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Nature 387:75-78.9169867
  • Hu, Y., Rolfs, A., Bhullar, B., Murthy, T. V., Zhu, C., Berger, M. F., Camargo, A. A., Kelley, F., McCarron, S., Jepson, D., Richardson, A., Raphael, J., Moreira, D., Taycher, E., Zuo, D., Mohr, S., Kane, M. F., Williamson, J., Simpson, A., Bulyk, M. L., Harlow, E., Marsischky, G., Kolodner, R. D., LaBaer, J. (2007). "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Genome Res 17:536-543.17322287
  • Toke, D. A., Bennett, W. L., Oshiro, J., Wu, W. I., Voelker, D. R., Carman, G. M. (1998). "Isolation and characterization of the Saccharomyces cerevisiae LPP1 gene encoding a Mg2+-independent phosphatidate phosphatase." J Biol Chem 273:14331-14338.9603941
  • Furneisen, J. M., Carman, G. M. (2000). "Enzymological properties of the LPP1-encoded lipid phosphatase from Saccharomyces cerevisiae." Biochim Biophys Acta 1484:71-82.10685032
  • Kihara, A., Sano, T., Iwaki, S., Igarashi, Y. (2003). "Transmembrane topology of sphingoid long-chain base-1-phosphate phosphatase, Lcb3p." Genes Cells 8:525-535.12786943
  • Huh, W. K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., O'Shea, E. K. (2003). "Global analysis of protein localization in budding yeast." Nature 425:686-691.14562095
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106