Vacuolar protein sorting-associated protein 30 (Q02948)

Identification
NameVacuolar protein sorting-associated protein 30
Synonyms
  • Autophagy-related protein 6
Gene NameVPS30
Enzyme ClassNot Available
Biological Properties
General Functionretrograde transport, endosome to Golgi
Specific FunctionRequired for cytoplasm to vacuole transport (Cvt), autophagy, nucleophagy, and mitophagy, as a part of the autophagy-specific VPS34 PI3-kinase complex I. This complex is essential to recruit the ATG8-phosphatidylinositol conjugate and the ATG12-ATG5 conjugate to the pre-autophagosomal structure. Also involved in endosome-to-Golgi retrograde transport as part of the VPS34 PI3-kinase complex II. This second complex is required for the endosome-to-Golgi retrieval of PEP1 and KEX2, and the recruitment of VPS5 and VPS7, two components of the retromer complex, to endosomal membranes (probably through the synthesis of a specific pool of phosphatidylinositol 3-phosphate recruiting the retromer to the endosomes). Plays also a role in regulation of filamentous growth.
Cellular LocationNot Available
SMPDB Pathways
Inositol MetabolismPW002498 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG PathwaysNot Available
SMPDB Reactions
1-Phosphatidyl-D-myo-inositol + Adenosine triphosphate → 1-Phosphatidyl-1D-myo-inositol 3-phosphate + ADP
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00109Adenosine triphosphateShow
YMDB002301-Phosphatidyl-D-myo-inositolShow
YMDB00914ADPShow
YMDB161651-Phosphatidyl-1D-myo-inositol 3-phosphateShow
GO Classification
Function
phosphatidylinositol 3-kinase complex, class III, type I
retrograde transport, endosome to Golgi
phosphatidylinositol 3-kinase complex, class III, type II
late endosome to vacuole transport
macroautophagy
pexophagy
protein targeting to vacuole
extrinsic component of membrane
cytoplasmic, membrane-bounded vesicle
pre-autophagosomal structure
pre-autophagosomal structure membrane
autophagosome assembly
autophagy
cellular response to nitrogen starvation
cytosol
CVT pathway
cytoplasm
phosphatidylinositol biosynthetic process
endosome membrane
piecemeal microautophagy of nucleus
Gene Properties
Chromosome LocationNot Available
Locus
Gene Sequence>1674 bp ATGAAGTGCCAAACATGTCACTTACCCCTGCAACTAGACCCATCTTTAGAAGGTCTTAGT TTAACCCAAAGAAATTTGCTACTTTCAAATAATTCAATTATCACTGCAACGAATGAAAAT GTCATCAGCAACAAGGGCATCGAAGCCGCAGATAATTGTGGGCCTCAAATACCGAAGGAG AGGCTGAGGCGATTAGGGGAGATTCAGAACATAAAAGACTTGAATTTAAAAGATGATAAA TTAATCACTGATTCGTTTGTTTTTCTTAATCACGATGATGATGATAATGCTAATATTACG AGTAATAGCAGAGAGGACCAGCGTTATGGAAATGCCAACGGAAATGATAATAAAAAAGCT AATAGTGATACTTCCGATGGAACCAGTACCTTTCGAGATCATGACGAAGAGGAACAAGAA GCTACGGATGAGGACGAAAACCAACAAATACAATTGAATTCCAAGACTTTGTCCACACAA GTCAACGCAATGACTAATGTATTTAACATTCTATCTTCTCAAACAAACATAGACTTTCCT ATCTGTCAGGACTGCTGTAACATCTTGATAAACAGACTAAAAAGTGAGTATGATGATGCA ATAAAAGAGAGAGATACTTACGCTCAATTTTTATCGAAGTTAGAGAGTCAAAACAAAGAA ATATCAGAAAGCAATAAAGAAAAGCAGTATTCCCATAATCTTTCGGAAAAAGAAAATTTG AAAAAGGAAGAGGAAAGACTCTTGGATCAATTATTACGTTTGGAAATGACAGATGATGAC TTAGATGGAGAACTAGTTCGTTTACAAGAAAAGAAAGTTCAACTGGAAAATGAGAAACTC CAAAAACTGAGTGATCAGAATTTAATGGATTTAAATAACATTCAATTCAACAAAAATTTA CAGTCATTAAAACTGCAATACGAATTATCCTTGAATCAATTAGATAAGTTGAGGAAAATC AATATTTTCAATGCTACATTCAAAATCTCACATAGCGGCCCCTTTGCCACTATAAATGGA TTGAGATTAGGCAGTATACCAGAATCAGTGGTACCTTGGAAGGAAATCAATGCAGCACTG GGCCAATTGATTCTTTTACTGGCTACCATAAACAAAAACCTGAAGATAAATTTAGTAGAC TACGAATTACAACCGATGGGTTCATTTTCCAAAATCAAGAAAAGAATGGTTAACAGTGTC GAATATAATAATTCCACTACGAACGCTCCCGGTGATTGGTTGATTCTGCCTGTCTACTAT GATGAGAATTTCAATTTGGGGAGAATCTTCCGCAAGGAAACGAAGTTTGATAAATCATTA GAAACAACATTAGAAATAATAAGCGAAATCACTCGGCAGCTTTCTACGATAGCGTCATCA TATTCATCTCAAACACTCACAACAAGCCAAGATGAATCTTCAATGAATAACGCAAATGAC GTTGAAAATAGTACTTCCATTCTCGAGCTACCCTATATCATGAATAAGGACAAGATTAAT GGATTATCCGTCAAATTGCATGGTAGCAGTCCCAACCTTGAATGGACAACGGCTATGAAA TTTTTATTAACCAATGTAAAGTGGTTGTTAGCGTTTTCTTCCAATTTACTATCCAAGTCA ATTACCTTAAGCCCTACAGTGAATTACAATGATAAGACCATCAGCGGAAACTAA
Protein Properties
Pfam Domain FunctionNot Available
Protein Residues557
Protein Molecular Weight63260.22
Protein Theoretical pINot Available
PDB Fileshow
Signalling RegionsNot Available
Transmembrane RegionsNot Available
Protein Sequence>Vacuolar protein sorting-associated protein 30 MKCQTCHLPLQLDPSLEGLSLTQRNLLLSNNSIITATNENVISNKGIEAADNCGPQIPKE RLRRLGEIQNIKDLNLKDDKLITDSFVFLNHDDDDNANITSNSREDQRYGNANGNDNKKA NSDTSDGTSTFRDHDEEEQEATDEDENQQIQLNSKTLSTQVNAMTNVFNILSSQTNIDFP ICQDCCNILINRLKSEYDDAIKERDTYAQFLSKLESQNKEISESNKEKQYSHNLSEKENL KKEEERLLDQLLRLEMTDDDLDGELVRLQEKKVQLENEKLQKLSDQNLMDLNNIQFNKNL QSLKLQYELSLNQLDKLRKINIFNATFKISHSGPFATINGLRLGSIPESVVPWKEINAAL GQLILLLATINKNLKINLVDYELQPMGSFSKIKKRMVNSVEYNNSTTNAPGDWLILPVYY DENFNLGRIFRKETKFDKSLETTLEIISEITRQLSTIASSYSSQTLTTSQDESSMNNAND VENSTSILELPYIMNKDKINGLSVKLHGSSPNLEWTTAMKFLLTNVKWLLAFSSNLLSKS ITLSPTVNYNDKTISGN
References
External Links
ResourceLink
Saccharomyces Genome Database VPS30
Uniprot IDQ02948
Uniprot NameVPS30
PDB ID
3VP7
General ReferenceNot Available