Bifunctional purine biosynthesis protein ADE16 (P54113)

Identification
NameBifunctional purine biosynthesis protein ADE16
Synonyms
  • Phosphoribosylaminoimidazolecarboxamide formyltransferase
  • 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase
  • AICAR transformylase
  • IMP cyclohydrolase
  • ATIC
  • IMP synthase
  • Inosinicase
Gene NameADE16
Enzyme Class
Biological Properties
General FunctionInvolved in IMP cyclohydrolase activity
Specific Function10-formyltetrahydrofolate + 5-amino-1-(5- phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5- formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
Cellular LocationCytoplasmic
SMPDB PathwaysNot Available
KEGG Pathways
Purine metabolismec00230 Map00230
SMPDB ReactionsNot Available
KEGG Reactions
Inosinic acid + water ↔ Phosphoribosyl formamidocarboxamide
5-Amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + N10-Formyl-THF ↔ 5,6,7,8-Tetrahydrofolic acid + Phosphoribosyl formamidocarboxamide
Metabolites
YMDB IDNameView
YMDB00080N10-Formyl-THFShow
YMDB00139Tetrahydrofolic acidShow
YMDB00281Phosphoribosyl formamidocarboxamideShow
YMDB00352Inosinic acidShow
YMDB004835,6,7,8-Tetrahydrofolic acidShow
YMDB0051710-Formyltetrahydrofolic acidShow
YMDB005625-Amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamideShow
YMDB00890waterShow
GO Classification
Component
Not Available
Function
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines
phosphoribosylaminoimidazolecarboxamide formyltransferase activity
cyclohydrolase activity
IMP cyclohydrolase activity
transferase activity, transferring one-carbon groups
catalytic activity
methyltransferase activity
transferase activity
glycine hydroxymethyltransferase activity
hydroxymethyl-, formyl- and related transferase activity
hydrolase activity
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
Process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleobase, nucleoside and nucleotide metabolic process
nucleoside phosphate metabolic process
nucleotide metabolic process
purine nucleotide metabolic process
purine nucleotide biosynthetic process
purine nucleoside monophosphate biosynthetic process
purine ribonucleoside monophosphate biosynthetic process
IMP biosynthetic process
metabolic process
nitrogen compound metabolic process
cellular nitrogen compound metabolic process
Gene Properties
Chromosome Locationchromosome 12
LocusYLR028C
Gene Sequence>1776 bp ATGGGCAAATATACCAAGACTGCGATCTTGTCAGTTTATGATAAGACAGGTTTATTAGAC TTAGCCAAGGGATTGGTAGAGAACAATGTTCGTATCCTAGCATCCGGTGGTACGGCCAAC ATGGTCAGAGAAGCTGGTTTTCCGGTCGACGATGTTTCTTCAATTACGCATGCACCAGAA ATGCTAGGGGGTAGAGTCAAGACTCTTCACCCTGCTGTGCATGCAGGTATTCTTGCCAGA AATTTAGAGGGCGATGAAAAAGATTTAAAAGAGCAGCACATTGATAAGGTGGATTTTGTT GTATGTAATCTATATCCGTTCAAAGAAACTGTGGCTAAAATTGGTGTTACAGTTCAAGAA GCTGTAGAGGAGATCGACATTGGCGGTGTGACTTTGTTGAGAGCAGCCGCAAAGAACCAT TCCAGAGTGACTATTCTTTCAGACCCTAATGACTATTCTATCTTTCTGCAAGACCTGTCT AAGGACGGTGAAATTTCTCAGGATTTAAGAAATAGATTTGCACTTAAGGCGTTTGAACAT ACGGCCGATTATGATGCGGCCATTTCTGACTTTTTCAGGAAACAGTACTCCGAAGGGAAA GCCCAATTACCGCTAAGATATGGTTGTAATCCCCACCAAAGACCTGCACAAGCTTATATC ACTCAGCAGGAGGAGTTGCCCTTCAAAGTGCTCTGCGGTACACCAGGATATATAAATCTT TTGGATGCCTTGAACTCATGGCCCTTAGTGAAAGAGTTATCTGCATCATTGAACTTGCCT GCGGCAGCATCGTTCAAGCACGTTTCTCCAGCAGGTGCTGCTGTGGGACTTCCATTATCC GATGTGGAAAGACAAGTTTATTTTGTGAATGATATGGAGGATTTATCACCATTGGCATGC GCATACGCTAGGGCCCGTGGTGCAGATAGGATGTCATCCTTTGGTGATTTCATTGCATTG TCCAATATTGTAGATGTCGCCACTGCAAAAATCATCTCTAAAGAAGTTTCCGATGGTGTT ATTGCTCCAGGGTATGAACCAGAGGCATTGAATATACTATCTAAGAAGAAAAACGGAAAA TACTGTATTTTACAAATCGATCCAAACTATGTTCCTGGCCAAATGGAATCCCGAGAGGTT TTTGGCGTAACGTTACAACAGAAGAGAAACGATGCGATCATAAACCAATCCACTTTCAAA GAAATTGTCTCCAAAAACAAAGCACTCACCGAGCAAGCCGTTATCGATCTAACTGTTGCT ACGCTGGTTTTAAAATACACACAATCAAATTCTGTTTGCTACGCAAAGAATGGTATGGTT GTGGGGCTTGGGGCCGGCCAACAATCGCGTATACACTGTACCAGATTAGCTGGTGACAAG ACGGACAACTGGTGGTTGAGACAACATCCAAAGGTTTTGAACATGAAGTGGGCCAAGGGA ATTAAGAGAGCGGACAAGTCGAATGCGATTGATCTTTTTGTTACAGGGCAAAGAATAGAA GGCCCGGAAAAGGTGGATTACGAAAGCAAATTTGAAGAAGTCCCTGAACCTTTCACCAAA GAAGAAAGACTAGAATGGTTAAGCAAATTAAACAATGTATCGTTATCATCCGATGCGTTC TTTCCCTTCCCAGACAACGTTTATAGAGCTGTGCAGTCTGGTGTAAAGTTCATCACGGCT CCTTCGGGGTCTGTCATGGACAAAGTTGTCTTCCAAGCGGCGGATTCATTTGATATCGTC TACGTGGAGAACCCAATTCGTTTATTCCACCACTAA
Protein Properties
Pfam Domain Function
Protein Residues591
Protein Molecular Weight65281.80078
Protein Theoretical pI6.52
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Bifunctional purine biosynthesis protein ADE16 MGKYTKTAILSVYDKTGLLDLAKGLVENNVRILASGGTANMVREAGFPVDDVSSITHAPE MLGGRVKTLHPAVHAGILARNLEGDEKDLKEQHIDKVDFVVCNLYPFKETVAKIGVTVQE AVEEIDIGGVTLLRAAAKNHSRVTILSDPNDYSIFLQDLSKDGEISQDLRNRFALKAFEH TADYDAAISDFFRKQYSEGKAQLPLRYGCNPHQRPAQAYITQQEELPFKVLCGTPGYINL LDALNSWPLVKELSASLNLPAAASFKHVSPAGAAVGLPLSDVERQVYFVNDMEDLSPLAC AYARARGADRMSSFGDFIALSNIVDVATAKIISKEVSDGVIAPGYEPEALNILSKKKNGK YCILQIDPNYVPGQMESREVFGVTLQQKRNDAIINQSTFKEIVSKNKALTEQAVIDLTVA TLVLKYTQSNSVCYAKNGMVVGLGAGQQSRIHCTRLAGDKTDNWWLRQHPKVLNMKWAKG IKRADKSNAIDLFVTGQRIEGPEKVDYESKFEEVPEPFTKEERLEWLSKLNNVSLSSDAF FPFPDNVYRAVQSGVKFITAPSGSVMDKVVFQAADSFDIVYVENPIRLFHH
References
External Links
ResourceLink
Saccharomyces Genome Database ADE16
Uniprot IDP54113
Uniprot NamePUR91_YEAST
GenBank Gene IDU62402
Genebank Protein ID1480728
General Reference
  • Johnston, M., Hillier, L., Riles, L., Albermann, K., Andre, B., Ansorge, W., Benes, V., Bruckner, M., Delius, H., Dubois, E., Dusterhoft, A., Entian, K. D., Floeth, M., Goffeau, A., Hebling, U., Heumann, K., Heuss-Neitzel, D., Hilbert, H., Hilger, F., Kleine, K., Kotter, P., Louis, E. J., Messenguy, F., Mewes, H. W., Hoheisel, J. D., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Nature 387:87-90.9169871
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106