Phosphatidylinositol N-acetylglucosaminyltransferase subunit GPI15 (P53961)

Identification
NamePhosphatidylinositol N-acetylglucosaminyltransferase subunit GPI15
Synonyms
  • PIGH homolog
Gene NameGPI15
Enzyme Class
Biological Properties
General FunctionInvolved in phosphatidylinositol N-acetylglucosaminyltransferase activity
Specific FunctionPart of the complex catalyzing the transfer of N- acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis
Cellular LocationMembrane; Multi-pass membrane protein (Potential)
SMPDB PathwaysNot Available
KEGG Pathways
Glycosylphosphatidylinositol(GPI)-anchor biosynthesisec00563 Map00563
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB002301-Phosphatidyl-D-myo-inositolShow
YMDB00277Uridine diphosphate-N-acetylglucosamineShow
YMDB00307Uridine 5'-diphosphateShow
GO ClassificationNot Available
Gene Properties
Chromosome Locationchromosome 14
LocusYNL038W
Gene Sequence>YNL038W GPI15 SGDID:S000004983, Chr XIV from 557020-557783, Verified ORF, "Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; h ATGATTAGTAAAGAGTATGAATTTGGTAAGACTAGTATACTGAATAGAAAGAAGTATACA TTAGTTATCGATGAAGACAAGAATGGCAATTTTATAAGATTTACCGTTTTACCTGTATCT AACCGAAAGTTCAAAAAAGTCAAGCAAAATGGGAGGGTAGAGATTAACATGGGCATACAA TATCACCAAATTGTACTTATTTTACTACTGAATATTTTGTTCTATGTAATTTGCCTAAGA TCAAGATTTCTCGAACATATTAATAGAACTTTTGAAGTGACAATCGCGCGAAGTTTCCAG ATCTTAATTATAATGGGATTGTTTGCCTTAGGTACAATTATACTTGTGAGGGGACCTAGT GTGGAAACTGTAACAATTTTCAAAGAAAGTGGACTACAGCTGTCCAGAGTGAAGGGTATG GTTATATTTCCTCAACAATGGAATCGGAAGTTCTTTGAACAAGTAGAGTTTATATCCAAT GAAAGAATTATTGATGTAGTGATCAATGAAGGATTCTGTCGGGGATTTCGAGTGATATTC TATCTTGCAGCAATTGTACGTAAATCGTCTACGCTTAAGCTATTATTTCCAGTATGTATT CAAGCGAATTTAAGATTGTTTCTTACTTCAACGATCTGATTAGAAATACTAACACAACAA TGCAGTCAAATTTGCCCAGTATCGATGACCAACGTCTAATATACAACATATCTAGAAAAT ATCTCAGTAAGCAAGAAAAACCCCTGAGCAGACCAAAAGATTGA
Protein Properties
Pfam Domain Function
Protein Residues229
Protein Molecular Weight26816.5
Protein Theoretical pI10.71
Signalling Regions
  • None
Transmembrane Regions
  • 59-79
  • 101-121
Protein Sequence>Phosphatidylinositol N-acetylglucosaminyltransferase subunit GPI15 MISKEYEFGKTSILNRKKYTLVIDEDKNGNFIRFTVLPVSNRKFKKVKQNGRVEINMGIQ YHQIVLILLLNILFYVICLRSRFLEHINRTFEVTIARSFQILIIMGLFALGTIILVRGPS VETVTIFKESGLQLSRVKGMVIFPQQWNRKFFEQVEFISNERIIDVVINEGFCRGFRVIF YLAAIVRKSSTLKLLFPSNLPSIDDQRLIYNISRKYLSKQEKPLSRPKD
References
External Links
ResourceLink
Saccharomyces Genome Database GPI15
Uniprot IDP53961
Uniprot NameGPI15_YEAST
General Reference
  • Philippsen, P., Kleine, K., Pohlmann, R., Dusterhoft, A., Hamberg, K., Hegemann, J. H., Obermaier, B., Urrestarazu, L. A., Aert, R., Albermann, K., Altmann, R., Andre, B., Baladron, V., Ballesta, J. P., Becam, A. M., Beinhauer, J., Boskovic, J., Buitrago, M. J., Bussereau, F., Coster, F., Crouzet, M., D'Angelo, M., Dal Pero, F., De Antoni, A., Del Rey, F., Doignon, F., Domdey, H., Dubois, E., Fiedler, T., Fleig, U., Floeth, M., Fritz, C., Gaillardin, C., Garcia-Cantalejo, J. M., Glansdorff, N. N., Goffeau, A., Gueldener, U., Herbert, C., Heumann, K., Heuss-Neitzel, D., Hilbert, H., Hinni, K., Iraqui Houssaini, I., Jacquet, M., Jimenez, A., Jonniaux, J. L., Karpfinger, L., Lanfranchi, G., Lepingle, A., Levesque, H., Lyck, R., Maftahi, M., Mallet, L., Maurer, K. C., Messenguy, F., Mewes, H. W., Mosti, D., Nasr, F., Nicaud, J. M., Niedenthal, R. K., Pandolfo, D., Pierard, A., Piravandi, E., Planta, R. J., Pohl, T. M., Purnelle, B., Rebischung, C., Remacha, M., Revuelta, J. L., Rinke, M., Saiz, J. E., Sartorello, F., Scherens, B., Sen-Gupta, M., Soler-Mira, A., Urbanus, J. H., Valle, G., Van Dyck, L., Verhasselt, P., Vierendeels, F., Vissers, S., Voet, M., Volckaert, G., Wach, A., Wambutt, R., Wedler, H., Zollner, A., Hani, J. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Nature 387:93-98.9169873
  • Hu, Y., Rolfs, A., Bhullar, B., Murthy, T. V., Zhu, C., Berger, M. F., Camargo, A. A., Kelley, F., McCarron, S., Jepson, D., Richardson, A., Raphael, J., Moreira, D., Taycher, E., Zuo, D., Mohr, S., Kane, M. F., Williamson, J., Simpson, A., Bulyk, M. L., Harlow, E., Marsischky, G., Kolodner, R. D., LaBaer, J. (2007). "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Genome Res 17:536-543.17322287
  • Yan, B. C., Westfall, B. A., Orlean, P. (2001). "Ynl038wp (Gpi15p) is the Saccharomyces cerevisiae homologue of human Pig-Hp and participates in the first step in glycosylphosphatidylinositol assembly." Yeast 18:1383-1389.11746600
  • Cliften, P., Sudarsanam, P., Desikan, A., Fulton, L., Fulton, B., Majors, J., Waterston, R., Cohen, B. A., Johnston, M. (2003). "Finding functional features in Saccharomyces genomes by phylogenetic footprinting." Science 301:71-76.12775844