Carbonic anhydrase (P53615)

Identification
NameCarbonic anhydrase
Synonyms
  • Carbonate dehydratase
  • Non-classical export protein 3
Gene NameNCE103
Enzyme Class
Biological Properties
General FunctionInvolved in carbonate dehydratase activity
Specific FunctionCatalyzes the reversible hydration of CO(2) to H(2)CO(3). The main role may be to provide inorganic carbon for the bicarbonate-dependent carboxylation reactions catalyzed by pyruvate carboxylase, acetyl-CoA carboxylase and carbamoyl- phosphate synthetase. Involved in protection against oxidative damage. Encodes a substrate for the non-classical protein export pathway for proteins that lack a cleavable signal sequence
Cellular LocationCytoplasm. Nucleus
SMPDB Pathways
Nitrogen metabolismPW002504 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
Nitrogen metabolismec00910 Map00910
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00382Carbonic acidShow
YMDB00912Carbon dioxideShow
GO Classification
Component
Not Available
Function
catalytic activity
lyase activity
binding
ion binding
cation binding
metal ion binding
transition metal ion binding
zinc ion binding
carbon-oxygen lyase activity
hydro-lyase activity
carbonate dehydratase activity
Process
carbon utilization
Gene Properties
Chromosome Locationchromosome 14
LocusYNL036W
Gene Sequence>666 bp ATGAGCGCTACCGAATCTTCATCTATATTCACATTGAGTCACAACTCAAACCTACAAGAT ATCTTGGCCGCCAATGCCAAATGGGCCTCCCAGATGAACAACATACAGCCAACTTGGTTC CCAGATCACAATGCGAAGGGCCAGTCCCCTCACACTCTTTTCATCGGCTGCTCCGATTCG CGTTACAACGAAAACTGTTTAGGTGTCTTGCCCGGCGAAGTGTTCACTTGGAAAAATGTT GCTAACATATGTCACTCAGAGGATTTAACTTTGAAGGCCACTTTAGAGTTTGCCATTATT TGTCTAAAAGTTAACAAAGTTATTATTTGTGGCCACACTGATTGTGGTGGTATAAAGACA TGTTTAACTAACCAAAGGGAAGCCTTACCAAAAGTTAACTGTTCTCATCTGTACAAGTAC TTAGACGATATTGACACCATGTACCATGAAGAGTCACAAAATTTGATCCATTTGAAAACG CAACGTGAAAAATCTCATTACCTGTCGCACTGTAACGTCAAAAGGCAGTTTAATAGGATT ATTGAAAACCCTACTGTGCAAACTGCTGTACAAAATGGAGAATTACAGGTATACGGTCTG CTTTACAACGTAGAGGACGGTCTACTGCAAACAGTTAGCACTTACACAAAAGTTACCCCA AAATAG
Protein Properties
Pfam Domain Function
Protein Residues221
Protein Molecular Weight24859.0
Protein Theoretical pI7.02
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Carbonic anhydrase MSATESSSIFTLSHNSNLQDILAANAKWASQMNNIQPTLFPDHNAKGQSPHTLFIGCSDS RYNENCLGVLPGEVFTWKNVANICHSEDLTLKATLEFAIICLKVNKVIICGHTDCGGIKT CLTNQREALPKVNCSHLYKYLDDIDTMYHEESQNLIHLKTQREKSHYLSHCNVKRQFNRI IENPTVQTAVQNGELQVYGLLYNVEDGLLQTVSTYTKVTPK
References
External Links
ResourceLink
Saccharomyces Genome Database NCE103
Uniprot IDP53615
Uniprot NameCAN_YEAST
GenBank Gene IDU52369
Genebank Protein ID1277232
General Reference
  • Cleves, A. E., Cooper, D. N., Barondes, S. H., Kelly, R. B. (1996). "A new pathway for protein export in Saccharomyces cerevisiae." J Cell Biol 133:1017-1026.8655575
  • Philippsen, P., Kleine, K., Pohlmann, R., Dusterhoft, A., Hamberg, K., Hegemann, J. H., Obermaier, B., Urrestarazu, L. A., Aert, R., Albermann, K., Altmann, R., Andre, B., Baladron, V., Ballesta, J. P., Becam, A. M., Beinhauer, J., Boskovic, J., Buitrago, M. J., Bussereau, F., Coster, F., Crouzet, M., D'Angelo, M., Dal Pero, F., De Antoni, A., Del Rey, F., Doignon, F., Domdey, H., Dubois, E., Fiedler, T., Fleig, U., Floeth, M., Fritz, C., Gaillardin, C., Garcia-Cantalejo, J. M., Glansdorff, N. N., Goffeau, A., Gueldener, U., Herbert, C., Heumann, K., Heuss-Neitzel, D., Hilbert, H., Hinni, K., Iraqui Houssaini, I., Jacquet, M., Jimenez, A., Jonniaux, J. L., Karpfinger, L., Lanfranchi, G., Lepingle, A., Levesque, H., Lyck, R., Maftahi, M., Mallet, L., Maurer, K. C., Messenguy, F., Mewes, H. W., Mosti, D., Nasr, F., Nicaud, J. M., Niedenthal, R. K., Pandolfo, D., Pierard, A., Piravandi, E., Planta, R. J., Pohl, T. M., Purnelle, B., Rebischung, C., Remacha, M., Revuelta, J. L., Rinke, M., Saiz, J. E., Sartorello, F., Scherens, B., Sen-Gupta, M., Soler-Mira, A., Urbanus, J. H., Valle, G., Van Dyck, L., Verhasselt, P., Vierendeels, F., Vissers, S., Voet, M., Volckaert, G., Wach, A., Wambutt, R., Wedler, H., Zollner, A., Hani, J. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Nature 387:93-98.9169873
  • Gotz, R., Gnann, A., Zimmermann, F. K. (1999). "Deletion of the carbonic anhydrase-like gene NCE103 of the yeast Saccharomyces cerevisiae causes an oxygen-sensitive growth defect." Yeast 15:855-864.10407265
  • Huh, W. K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., O'Shea, E. K. (2003). "Global analysis of protein localization in budding yeast." Nature 425:686-691.14562095
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Clark, D., Rowlett, R. S., Coleman, J. R., Klessig, D. F. (2004). "Complementation of the yeast deletion mutant DeltaNCE103 by members of the beta class of carbonic anhydrases is dependent on carbonic anhydrase activity rather than on antioxidant activity." Biochem J 379:609-615.15096093
  • Aguilera, J., Van Dijken, J. P., De Winde, J. H., Pronk, J. T. (2005). "Carbonic anhydrase (Nce103p): an essential biosynthetic enzyme for growth of Saccharomyces cerevisiae at atmospheric carbon dioxide pressure." Biochem J 391:311-316.15948716
  • Aguilera, J., Petit, T., de Winde, J. H., Pronk, J. T. (2005). "Physiological and genome-wide transcriptional responses of Saccharomyces cerevisiae to high carbon dioxide concentrations." FEMS Yeast Res 5:579-593.15780657
  • Amoroso, G., Morell-Avrahov, L., Muller, D., Klug, K., Sultemeyer, D. (2005). "The gene NCE103 (YNL036w) from Saccharomyces cerevisiae encodes a functional carbonic anhydrase and its transcription is regulated by the concentration of inorganic carbon in the medium." Mol Microbiol 56:549-558.15813743