| Identification |
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| Name | Phosphatidylinositol N-acetylglucosaminyltransferase subunit GPI1 |
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| Synonyms | Not Available |
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| Gene Name | GPI1 |
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| Enzyme Class | |
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| Biological Properties |
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| General Function | Involved in phosphatidylinositol N-acetylglucosaminyltransferase activity |
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| Specific Function | Part of the complex catalyzing the transfer of N- acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis |
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| Cellular Location | Membrane; Multi-pass membrane protein (Potential) |
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| SMPDB Pathways | Not Available |
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| KEGG Pathways | | Glycosylphosphatidylinositol(GPI)-anchor biosynthesis | ec00563 |  |
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| SMPDB Reactions | Not Available |
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| KEGG Reactions | Not Available |
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| Metabolites | | YMDB ID | Name | View |
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| YMDB00230 | 1-Phosphatidyl-D-myo-inositol | Show | | YMDB00277 | Uridine diphosphate-N-acetylglucosamine | Show | | YMDB00307 | Uridine 5'-diphosphate | Show |
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| GO Classification | | Component |
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| cell part | | membrane part | | intrinsic to membrane | | integral to membrane | | Function |
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| UDP-glycosyltransferase activity | | acetylglucosaminyltransferase activity | | phosphatidylinositol N-acetylglucosaminyltransferase activity | | catalytic activity | | transferase activity | | transferase activity, transferring glycosyl groups | | Process |
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| GPI anchor biosynthetic process | | metabolic process | | macromolecule metabolic process | | macromolecule modification | | protein modification process | | protein amino acid lipidation |
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| Gene Properties |
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| Chromosome Location | chromosome 7 |
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| Locus | YGR216C |
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| Gene Sequence | >1830 bp
ATGCCAAATTACATTTTCTGGCCATACGAATCCTTGTTCGAGAATTCCGCAGCTCAAGGA
CCTCAGGTAGCTTTGGCAATTTCATTTGAGAAGACGCATTTTGTTGTCTTAGGTGTTTGC
GAACCTCAGTATTTAGAAGAAGTAAGCATACGGCCCCCTTATTCAGTGGTAGCTACTAAG
AACAATGGAGCGGAGGGTTGGAACTACAAAGTTGCTGATCCCTGTAATGTCCATTTTAGA
ATACCCAAGCTGAAATTTATGCAATTCTATTCTTCTGATCCCATCTCGCTCATCATACCA
GAAAAAGAAGTTGGTCTTCATAGTAGTGTGGGTGAAACTCTGAACTATTCTAAATTAGAG
CAACATCCAAGATACAAGAGAGATAATAAGAAATTGAGTGAAACTTTGAACATCATTAAT
CTATTCCCCGCATATTGTAAAGCATTAAATGAGCTTTACCCGTTTATTCAAACATCACAG
GAAAATTTGAGGGGTACTATGCTTAATAGTGTCGCAGCTTGGTGTTCATCTACTTGTATT
TATAAAATGGTCGCAAAAATTGGTTTTTATTTGACTTTTGTGATCTGCTCTATTGCTAGT
CTCGTATCCTCGTTATTAAATTATTCGCACTTTCAGTTGGTAAATTACTCGGCATTTGTC
CAGCAAATCGATCTTCGTTGTCAACAAATTTGCTATTTCCCAGTACAGTATGAGCGTATT
AATAAGAAGGATAACATACAGAATGTTGGCTCTATGGTGGAAAAAGATAATTCTAATAGT
CAATTCTCACATTCATATATGCCTTCTAAATTCTATCCCGATTATATTTTATTGTATAAT
ACAATTTGGCTTATTATTAATGACATTTCATTTGGTTTAATATTGGGGGCCATACTTATT
GAGAATCGAGATTTCTTGGTGTCGGCATCCCATAGAGTATTGAAGTTTTTTTTATATGAT
TCATTAAAAACAATAACTGAAACGTTAGCAAATAACCCCCTTGGCATCAAATTAAATGCA
GAACTTGCGAACTTTTTGAGCGAATTATTTCTTTGGGTGATAGAATTTTCTTATACCACA
TTCATAAAAAGACTCATTGATCCGAAAACCCTATCATCTTTGCTCACGTTAACGATATAT
ATGATGTTTCTCGTTGGCTTTTCTTTTGCTGTTTCCCTAGCGATTGATTTTTTTGCAATT
TTAAGTTTTCCAATATATGTTTTTTATCGTATCAGCAGTAAACTGTACCATTGTCAGTTG
AATATTATGGCTAGTCTTTTTAACCTGTTCTGCGGTAAAAAGAGGAACGTTTTACGAAAT
AGGATTGACCATAACTATTTTCAACTAGATCAATTGCTATTGGGAACATTATTATTTATT
ATTCTAGTGTTCTTGACTCCCACAGTGATGGCATTCTATATGTCATACACAGTTTTGAGA
ATGCTAACAATAACTATCGAAATATTCTCGGAAGCTGTCATTGCACTCATTAACCATTTT
CCATTGTTCGCATTATTACTTAGGTTAAAAGATCCTAAACGTCTACCCGGTGGCATTTCA
ATTGAGCTCAAAACCACGGTTTCTAACAAGCATACAACCTTAGAACTTCAAAACAATCCT
ATTAAATTCAAGAGTATGTTCAGACCATACAATCTATTGTTGTCGCAAATGAGGACAAAT
TATTTCTCGTTTGCTACTGTACGAAAGATTGTCCGAGGGGAGTCTATAATGGTCAATAGA
AATAAGCTGTACTATGTTCTTTATTCTTCACTGCCTAGTAAGCCATTGAGTGTAAAGGAT
TTATACAAAAGGCTGACTATTCAGGCATAA |
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| Protein Properties |
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| Pfam Domain Function | |
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| Protein Residues | 609 |
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| Protein Molecular Weight | 70353.70313 |
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| Protein Theoretical pI | 9.4 |
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| Signalling Regions | |
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| Transmembrane Regions | - 187-207
- 281-301
- 381-401
- 452-472
- 486-506
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| Protein Sequence | >Phosphatidylinositol N-acetylglucosaminyltransferase subunit GPI1
MPNYIFWPYESLFENSAAQGPQVALAISFEKTHFVVLGVCEPQYLEEVSIRPPYSVVATK
NNGAEGWNYKVADPCNVHFRIPKLKFMQFYSSDPISLIIPEKEVGLHSSVGETLNYSKLE
QHPRYKRDNKKLSETLNIINLFPAYCKALNELYPFIQTSQENLRGTMLNSVAAWCSSTCI
YKMVAKIGFYLTFVICSIASLVSSLLNYSHFQLVNYSAFVQQIDLRCQQICYFPVQYERI
NKKDNIQNVGSMVEKDNSNSQFSHSYMPSKFYPDYILLYNTIWLIINDISFGLILGAILI
ENRDFLVSASHRVLKFFLYDSLKTITETLANNPLGIKLNAELANFLSELFLWVIEFSYTT
FIKRLIDPKTLSSLLTLTIYMMFLVGFSFAVSLAIDFFAILSFPIYVFYRISSKLYHCQL
NIMASLFNLFCGKKRNVLRNRIDHNYFQLDQLLLGTLLFIILVFLTPTVMAFYMSYTVLR
MLTITIEIFSEAVIALINHFPLFALLLRLKDPKRLPGGISIELKTTVSNKHTTLELQNNP
IKFKSMFRPYNLLLSQMRTNYFSFATVRKIVRGESIMVNRNKLYYVLYSSLPSKPLSVKD
LYKRLTIQA |
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| References |
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| External Links | |
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| General Reference | - Leidich, S. D., Orlean, P. (1996). "Gpi1, a Saccharomyces cerevisiae protein that participates in the first step in glycosylphosphatidylinositol anchor synthesis." J Biol Chem 271:27829-27837.8910381
- Rieger, M., Bruckner, M., Schafer, M., Muller-Auer, S. (1997). "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII." Yeast 13:1077-1090.9290212
- Tettelin, H., Agostoni Carbone, M. L., Albermann, K., Albers, M., Arroyo, J., Backes, U., Barreiros, T., Bertani, I., Bjourson, A. J., Bruckner, M., Bruschi, C. V., Carignani, G., Castagnoli, L., Cerdan, E., Clemente, M. L., Coblenz, A., Coglievina, M., Coissac, E., Defoor, E., Del Bino, S., Delius, H., Delneri, D., de Wergifosse, P., Dujon, B., Kleine, K., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Nature 387:81-84.9169869
- Sobering, A. K., Watanabe, R., Romeo, M. J., Yan, B. C., Specht, C. A., Orlean, P., Riezman, H., Levin, D. E. (2004). "Yeast Ras regulates the complex that catalyzes the first step in GPI-anchor biosynthesis at the ER." Cell 117:637-648.15163411
- Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258
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