Alpha-1,2 glucosyltransferase ALG10 (P50076)

Identification
NameAlpha-1,2 glucosyltransferase ALG10
Synonyms
  • Alpha-2-glucosyltransferase ALG10
  • Asparagine-linked glycosylation protein 10
  • Dolichyl-phosphoglucose-dependent glucosyltransferase ALG10
Gene NameDIE2
Enzyme Class
Biological Properties
General FunctionInvolved in transferase activity, transferring hexosyl groups
Specific FunctionAdds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc(2)Man(9)GlcNAc(2)-PP-Dol
Cellular LocationEndoplasmic reticulum membrane; Multi-pass membrane protein
SMPDB Pathways
N-Glycan biosynthesisPW002501 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
N-Glycan biosynthesisec00510 Map00510
SMPDB Reactions
(Glucosyl)2(mannosyl)9-(N-acetylglucosaminyl)2-diphosphodolichol + Dolichyl b-D-glucosyl phosphate → hydron + Dolichol phosphate + (glucosyl)3(mannosyl)9-(N-acetylglucosaminyl)2-diphosphodolichol
KEGG Reactions
D-Glucose + glycan G10599 ↔ glycan G00008
Metabolites
YMDB IDNameView
YMDB00241Dolichyl b-D-glucosyl phosphateShow
YMDB00286D-GlucoseShow
YMDB00862hydronShow
YMDB00864Dolichol phosphateShow
YMDB00954glycan G00008Show
YMDB00955glycan G10599Show
YMDB16224(Glucosyl)2(mannosyl)9-(N-acetylglucosaminyl)2-diphosphodolicholShow
YMDB16338(glucosyl)3(mannosyl)9-(N-acetylglucosaminyl)2-diphosphodolicholShow
GO Classification
Component
cell part
membrane part
intrinsic to membrane
integral to membrane
Function
catalytic activity
transferase activity
transferase activity, transferring glycosyl groups
transferase activity, transferring hexosyl groups
Process
Not Available
Gene Properties
Chromosome Locationchromosome 7
LocusYGR227W
Gene Sequence>1578 bp ATGGATGCAAAGAAAAATACTGGTGAAGCCAATAATGATGTATTGGAAGAGGAAGCAGCG ATTCAGTTGATTGCACCAGGTATAGCGAGAAACTTAACACAAGAAGTCATTACCGGTATC TTCTGTAATGTAGTAATATATCCTCTTCTTCTAATATATTTTGTCTTAACTTTCCGTTAC ATGACTACAAATATTGTACCTTATGAATTTATAGATGAAAAGTTTCACGTTGGCCAAACT TTGACCTATTTAAAAGGTAAATGGACTCAATGGGACCCTAAAATAACAACCCCCCCTGGA ATCTATATTTTGGGTTTAATTAATTATTATTGTATAAAGCCCATATTCAAGTCGTGGAGC ACACTAACCATACTAAGATTAGTTAATTTACTTGGAGGGATTATCGTTTTCCCCATTTTA GTACTCAGGCCAATATTCCTTTTTAATGCCTTAGGTTTTTGGCCCGTTTCATTAATGAGC TTCCCATTGATGACAACTTATTATTACTTATTTTACACCGATGTGTGGTCTACAATTCTG ATATTGCAATCACTCAGCTGCGTGTTAACATTACCGTTTGGCCCCGTGAAGAGTATATGG TTGAGTGCTTTTTTTGCTGGTGTAAGCTGTCTCTTCAGACAAACGAATATCATTTGGACG GGATTTATAATGATTCTTGCTGTTGAACGCCCAGCCATTTTACAAAAACAGTTCAACACG CATACGTTCAATAACTATTTGAAATTATTCATTCATGCTATTGACGATTTCTCCAACCTT GTTCTGCCCTATATGATAAATTTTGTTTTGTTCTTCATTTATCTGATTTGGAACAGATCC ATAACACTAGGTGATAAGTCCAGCCACTCAGCAGGCCTGCATATAGTACAAATATTCTAT TGTTTCACATTCATCACCGTTTTCAGTTTACCTATATGGATATCTCGTAATTTTATGAAG TTGTACAAATTAAGGATAAAACGGAAGCCAGTACAAACTTTTTTTGAATTTATTGGTATT ATGCTAATCATAAGATACTTTACAAAAGTTCACCCATTTTTGTTAGCAGATAATAGACAC TACACGTTCTACCTCTTCAGAAGATTGATAGGCAACAAAAGTAGACTAATCAAGTACTTT TTTATGACACCAATCTATCATTTCTCCACTTTCGCATACCTTGAAGTGATGAGACCCAAC CAGCTCACCTTTCACCCCATCACTCCACTCCCCATCAAAGAACCTGTACATTTACCCATA CAACTGACACATGTATCGTGGACGGCCTTAATAACGTGCACCATGGTAACTATCGTTCCA TCACCCTTATTCGAACCTCGTTACTATATATTGCCCTATTTCTTTTGGAGAATATTTATT ACCTGCAGCTGCGAACCTTTGATAAAGGATTTAAAACCTGCTAAAGAAGGCGAAAACCCA ATTACCATAAGCAGTACGAAAAGACTGTTCATGGAATTTTTATGGTTCATGCTCTTCAAT GTCGTTACTTTAGTTATCTTTTCCAAGGTCTCATTTCCTTGGACCACTGAGCCCTATTTG CAAAGAATAATATGGTGA
Protein Properties
Pfam Domain Function
Protein Residues525
Protein Molecular Weight61807.0
Protein Theoretical pI9.75
Signalling Regions
  • None
Transmembrane Regions
  • 36-56
  • 95-115
  • 128-148
  • 151-171
  • 188-208
  • 214-234
  • 257-277
  • 295-315
  • 334-354
  • 376-396
  • 424-444
  • 448-468
  • 493-513
Protein Sequence>Alpha-1,2 glucosyltransferase ALG10 MDAKKNTGEANNDVLEEEAAIQLIAPGIARNLTQEVITGIFCNVVIYPLLLIYFVLTFRY MTTNIVPYEFIDEKFHVGQTLTYLKGKWTQWDPKITTPPGIYILGLINYYCIKPIFKSWS TLTILRLVNLLGGIIVFPILVLRPIFLFNALGFWPVSLMSFPLMTTYYYLFYTDVWSTIL ILQSLSCVLTLPFGPVKSIWLSAFFAGVSCLFRQTNIIWTGFIMILAVERPAILQKQFNT HTFNNYLKLFIHAIDDFSNLVLPYMKNFVLFFIYLIWNRSITLGDKSSHSAGLHIVQIFY CFTFITVFSLPIWISRNFMKLYKLRIKRKPVQTFFEFIGIMLIIRYFTKVHPFLLADNRH YTFYLFRRLIGNKSRLIKYFFMTPIYHFSTFAYLEVMRPNQLTFHPITPLPIKEPVHLPI QLTHVSWTALITCTMVTIVPSPLFEPRYYILPYFFWRIFITCSCEPLIKDLKPAKEGENP ITISSTKRLFMEFLWFMLFNVVTLVIFSKVSFPWTTEPYLQRIIW
References
External Links
ResourceLink
Saccharomyces Genome Database DIE2
Uniprot IDP50076
Uniprot NameALG10_YEAST
GenBank Gene IDD38049
Genebank Protein ID902940
General Reference
  • Nikawa, J., Hosaka, K. (1995). "Isolation and characterization of genes that promote the expression of inositol transporter gene ITR1 in Saccharomyces cerevisiae." Mol Microbiol 16:301-308.7565092
  • van der Aart, Q. J., Kleine, K., Steensma, H. Y. (1996). "Sequence analysis of the 43 kb CRM1-YLM9-PET54-DIE2-SMI1-PHO81-YHB4-PFK1 region from the right arm of Saccharomyces cerevisiae chromosome VII." Yeast 12:385-390.8701610
  • Tettelin, H., Agostoni Carbone, M. L., Albermann, K., Albers, M., Arroyo, J., Backes, U., Barreiros, T., Bertani, I., Bjourson, A. J., Bruckner, M., Bruschi, C. V., Carignani, G., Castagnoli, L., Cerdan, E., Clemente, M. L., Coblenz, A., Coglievina, M., Coissac, E., Defoor, E., Del Bino, S., Delius, H., Delneri, D., de Wergifosse, P., Dujon, B., Kleine, K., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Nature 387:81-84.9169869
  • Burda, P., Aebi, M. (1998). "The ALG10 locus of Saccharomyces cerevisiae encodes the alpha-1,2 glucosyltransferase of the endoplasmic reticulum: the terminal glucose of the lipid-linked oligosaccharide is required for efficient N-linked glycosylation." Glycobiology 8:455-462.9597543