Glutamyl-tRNA synthetase, mitochondrial (P48525)

Identification
NameGlutamyl-tRNA synthetase, mitochondrial
Synonyms
  • Glutamate--tRNA ligase
  • GluRS
Gene NameMSE1
Enzyme Class
Biological Properties
General FunctionInvolved in nucleotide binding
Specific FunctionCatalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)
Cellular LocationMitochondrion matrix
SMPDB PathwaysNot Available
KEGG Pathways
Porphyrin and chlorophyll metabolismec00860 Map00860
SMPDB ReactionsNot Available
KEGG Reactions
Adenosine triphosphate + tRNA(Glu) + L-Glutamic acid → Adenosine monophosphate + Pyrophosphate + Glu-tRNA(Glu)
Metabolites
YMDB IDNameView
YMDB00097Adenosine monophosphateShow
YMDB00109Adenosine triphosphateShow
YMDB00219PyrophosphateShow
YMDB00271L-Glutamic acidShow
GO Classification
Component
intracellular part
cytoplasm
cell part
Function
binding
nucleoside binding
purine nucleoside binding
glutamate-tRNA ligase activity
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
ligase activity, forming carbon-oxygen bonds
ligase activity, forming aminoacyl-tRNA and related compounds
catalytic activity
aminoacyl-tRNA ligase activity
nucleotide binding
ligase activity
Process
biosynthetic process
tRNA metabolic process
tRNA aminoacylation
tRNA aminoacylation for protein translation
macromolecule biosynthetic process
cellular macromolecule biosynthetic process
translation
glutamyl-tRNA aminoacylation
metabolic process
macromolecule metabolic process
cellular macromolecule metabolic process
RNA metabolic process
ncRNA metabolic process
Gene Properties
Chromosome Locationchromosome 15
LocusYOL033W
Gene Sequence>1611 bp ATGATCATGTTGAGAATACCGACACGGTCTTATTGTTCACCTTCGAAGCTAATAAAGGGC GTAGGTCTCTCACCATTGAAGAAATCTCTGTTATCCAAGAAAATAAAGGAAGATATCCAT CCTTCTTTACCTGTTAGAACTAGGTTTGCGCCTTCTCCAACTGGGTTTTTACACTTGGGG TCACTGAGAACAGCCCTTTACAATTACCTATTAGCAAGAAACACCAATGGGCAGTTTTTG CTTCGTTTAGAAGATACAGACCAGAAGAGGCTAATAGAGGGAGCAGAAGAAAACATCTAC GAGATTCTGAAATGGTGTAACATAAACTACGATGAAACCCCTATAAAGCAAAGTGAAAGG AAACTAATATATGACAAATATGTAAAAATATTACTATCTTCTGGGAAGGCGTATAGATGT TTTTGTTCAAAGGAGCGATTGAACGATTTGAGACACTCTGCAATGGAATTGAAACCTCCT TCGATGGCAAGCTATGATCGATGTTGTGCACACTTAGGGGAGGAAGAAATAAAATCTAAG CTAGCGCAAGGTATACCTTTCACTGTGCGGTTTAAATCGCCAGAACGATATCCAACGTTT ACTGATCTTTTACATGGACAAATAAATCTTCAACCGCAAGTAAACTTTAACGATAAACGG TACGATGACCTTATCCTTGTTAAGTCGGATAAGTTGCCAACCTATCATCTGGCAAATGTT GTGGATGACCATTTGATGGGAATAACCCATGTTATAAGAGGTGAGGAATGGCTGCCCTCT ACTCCTAAACATATCGCCCTATATAATGCATTTGGGTGGGCTTGCCCAAAGTTCATCCAC ATTCCCTTATTGACGACGGTTGGTGATAAGAAATTGAGTAAAAGAAAAGGTGATATGTCT ATCTCGGATTTAAAAAGACAGGGAGTTTTGCCAGAAGCTTTGATTAACTTTTGTGCGCTA TTTGGCTGGTCTCCTCCAAGAGATCTGGCTTCGAAAAAGCATGAGTGTTTTTCAATGGAG GAATTGGAAACCATTTTTAATTTGAATGGTTTGACGAAGGGAAATGCCAAAGTCGATGAT AAAAAATTGTGGTTCTTCAATAAGCATTTTTTGCAAAAAAGAATACTTAATCCTTCTACA TTGAGGGAGCTTGTCGACGACATTATGCCTTCTTTAGAGTCCATATATAACACATCTACC ATAAGCCGTGAGAAAGTAGCTAAAATTTTACTGAACTGTGGTGGTTCTCTATCAAGGATT AACGATTTTCATGATGAATTTTATTATTTTTTTGAAAAGCCCAAATATAATGATAATGAT GCTGTCACGAAATTTCTAAGCAAGAACGAAAGCCGCCACATTGCTCACTTATTGAAGAAG CTTGGACAGCTTCAAGAGGGCACCGATGCTCAAGAAGTCGAATCTATGGTCGAAACGATG TATTATGAGAACGGTTTTTCTAGGAAAGTCACGTATCAGGCAATGAGATTTGCTCTTGCG GGATGTCACCCAGGTGCCAAAATAGCAGCCATGATAGATATTTTGGGCATCAAGGAAAGT AACAAAAGATTATCCGAAGGTTTACAGTTCTTGCAAAGAGAGAAAAAGTAA
Protein Properties
Pfam Domain Function
Protein Residues536
Protein Molecular Weight61602.69922
Protein Theoretical pI9.48
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Glutamyl-tRNA synthetase, mitochondrial MIMLRIPTRSYCSPSKLIKGVGLSPLKKSLLSKKIKEDIHPSLPVRTRFAPSPTGFLHLG SLRTALYNYLLARNTNGQFLLRLEDTDQKRLIEGAEENIYEILKWCNINYDETPIKQSER KLIYDKYVKILLSSGKAYRCFCSKERLNDLRHSAMELKPPSMASYDRCCAHLGEEEIKSK LAQGIPFTVRFKSPERYPTFTDLLHGQINLQPQVNFNDKRYDDLILVKSDKLPTYHLANV VDDHLMGITHVIRGEEWLPSTPKHIALYNAFGWACPKFIHIPLLTTVGDKKLSKRKGDMS ISDLKRQGVLPEALINFCALFGWSPPRDLASKKHECFSMEELETIFNLNGLTKGNAKVDD KKLWFFNKHFLQKRILNPSTLRELVDDIMPSLESIYNTSTISREKVAKILLNCGGSLSRI NDFHDEFYYFFEKPKYNDNDAVTKFLSKNESRHIAHLLKKLGQFQEGTDAQEVESMVETM YYENGFSRKVTYQAMRFALAGCHPGAKIAAMIDILGIKESNKRLSEGLQFLQREKK
References
External Links
ResourceLink
Saccharomyces Genome Database MSE1
Uniprot IDP48525
Uniprot NameSYEM_YEAST
GenBank Gene IDL39015
Genebank Protein ID625182
General Reference
  • Dujon, B., Albermann, K., Aldea, M., Alexandraki, D., Ansorge, W., Arino, J., Benes, V., Bohn, C., Bolotin-Fukuhara, M., Bordonne, R., Boyer, J., Camasses, A., Casamayor, A., Casas, C., Cheret, G., Cziepluch, C., Daignan-Fornier, B., Dang, D. V., de Haan, M., Delius, H., Durand, P., Fairhead, C., Feldmann, H., Gaillon, L., Kleine, K., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Nature 387:98-102.9169874
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106