GPI mannosyltransferase 1 (P47088)

Identification
NameGPI mannosyltransferase 1
Synonyms
  • GPI mannosyltransferase I
  • GPI-MT-I
  • Glycosylphosphatidylinositol-anchor biosynthesis protein 14
Gene NameGPI14
Enzyme Class
Biological Properties
General FunctionInvolved in transferase activity, transferring hexosyl groups
Specific FunctionMannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the first alpha-1,4-mannose to GlcN-acyl-PI during GPI precursor assembly. Required for cell wall integrity
Cellular LocationEndoplasmic reticulum membrane; Multi-pass membrane protein
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00937dolichyl D-mannosyl phosphateShow
YMDB00941dolichyl phosphateShow
GO Classification
Component
cell part
membrane
membrane part
intrinsic to membrane
integral to membrane
organelle membrane
endoplasmic reticulum membrane
Function
transferase activity, transferring hexosyl groups
catalytic activity
transferase activity
transferase activity, transferring glycosyl groups
Process
metabolic process
macromolecule metabolic process
macromolecule modification
protein modification process
protein amino acid lipidation
GPI anchor biosynthetic process
Gene Properties
Chromosome Locationchromosome 10
LocusYJR013W
Gene Sequence>YJR013W GPI14 SGDID:S000003774, Chr X from 460382-461593, Verified ORF, "Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M" ATGACTGGCGAAGAATGGGGCTTGACTGTACTGTCATTCTTGGTAAGAGTCGGATTTTTT CTTTTTGGCATTTACCAGGACGCTAATTTCAAAGTGAGGTACACTGATATCGACTATTTT GTGTTTCATGATGCTGCTAAATATGTATACGAAGGCAAATCACCTTATGCAAGGGATACA TATCGTTACACACCGCTACTAAGTTGGCTGCTAGTGCCTAATCATTATTTTGGATGGTTT CATTTGGGGAAGGTCATATTTGTTATTTTCGATTTGGTTACTGGGCTCATTATAATGAAG CTTTTGAACCAGGCCATCTCCAGAAAAAGAGCTTTAATATTGGAAAGCATTTGGCTATTG AATCCGATGGTTATCACCATCAGTACAAGGGGAAACGCTGAAAGCGTGCTTTGCTGTTTG ATCATGTTCACGCTTTTTTTTCTACAAAAGAGTAGATACACCCTAGCTGGAATCTTATAT GGTCTTTCTATCCATTTCAAAATATATCCAATCATATATTGTATCCCAATAGCAATTTTT ATATACTACAATAAAAGAAACCAGGGTCCACGAACACAACTTACATCGCTACTCAATATC GGTTTGAGTACTCTAACAACTTTGCTTGGATGTGGTTGGGCAATGTACAAGATATACGGA TACGAGTTTCTGGATCAGGCATACTTGTACCACTTATACAGAACGGATCACAGACACAAT TTTTCAGTGTGGAACATGCTATTGTATTTAGATTCTGCAAATAAGGAAAATGGTGAGTCC AATCTCTCAAGGTACGCATTTGTGCCTCAATTGCTGCTTGTTTTAGTAACTGGATGTCTT GAATGGTGGAATCCCACATTCGATAACCTATTGAGGGTTTTATTTGTGCAGACGTTCGCA TTTGTGACATATAATAAAGTGTGCACATCGCAATATTTTGTTTGGTACCTGATCTTTCTA CCATTTTACCTCTCAAGAACGCACATTGGTTGGAAGAAGGGGCTTTTAATGGCCACGCTT TGGGTAGGAACGCAGGGAATTTGGTTAAGCCAAGGTTACTATTTGGAATTCGAAGGCAAG AATGTATTCTATCCTGGACTATTTATTGCAAGCGTGTTATTTTTCGTCACCAATGTGTGG CTACTGGGTCAATTTATTACTGATATTAAGATACCGACGCAACCCACAGTTTCCAACAAA AAGAACAACTGA
Protein Properties
Pfam Domain Function
Protein Residues403
Protein Molecular Weight47165.89844
Protein Theoretical pI9.33
Signalling Regions
  • None
Transmembrane Regions
  • 5-25
  • 79-99
  • 111-131
  • 133-149
  • 161-181
  • 194-214
  • 267-287
  • 311-331
  • 335-355
  • 362-382
Protein Sequence>GPI mannosyltransferase 1 MTGEEWGLTVLSFLVRVGFFLFGIYQDANFKVRYTDIDYFVFHDAAKYVYEGKSPYARDT YRYTPLLSWLLVPNHYFGWFHLGKVIFVIFDLVTGLIIMKLLNQAISRKRALILESIWLL NPMVITISTRGNAESVLCCLIMFTLFFLQKSRYTLAGILYGLSIHFKIYPIIYCIPIAIF IYYNKRNQGPRTQLTSLLNIGLSTLTTLLGCGWAMYKIYGYEFLDQAYLYHLYRTDHRHN FSVWNMLLYLDSANKENGESNLSRYAFVPQLLLVLVTGCLEWWNPTFDNLLRVLFVQTFA FVTYNKVCTSQYFVWYLIFLPFYLSRTHIGWKKGLLMATLWVGTQGIWLSQGYYLEFEGK NVFYPGLFIASVLFFVTNVWLLGQFITDIKIPTQPTVSNKKNN
References
External Links
ResourceLink
Saccharomyces Genome Database GPI14
Uniprot IDP47088
Uniprot NameGPI14_YEAST
General Reference
  • Galibert, F., Alexandraki, D., Baur, A., Boles, E., Chalwatzis, N., Chuat, J. C., Coster, F., Cziepluch, C., De Haan, M., Domdey, H., Durand, P., Entian, K. D., Gatius, M., Goffeau, A., Grivell, L. A., Hennemann, A., Herbert, C. J., Heumann, K., Hilger, F., Hollenberg, C. P., Huang, M. E., Jacq, C., Jauniaux, J. C., Katsoulou, C., Karpfinger-Hartl, L., et, a. l. .. (1996). "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." EMBO J 15:2031-2049.8641269
  • Hu, Y., Rolfs, A., Bhullar, B., Murthy, T. V., Zhu, C., Berger, M. F., Camargo, A. A., Kelley, F., McCarron, S., Jepson, D., Richardson, A., Raphael, J., Moreira, D., Taycher, E., Zuo, D., Mohr, S., Kane, M. F., Williamson, J., Simpson, A., Bulyk, M. L., Harlow, E., Marsischky, G., Kolodner, R. D., LaBaer, J. (2007). "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Genome Res 17:536-543.17322287
  • Huh, W. K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., O'Shea, E. K. (2003). "Global analysis of protein localization in budding yeast." Nature 425:686-691.14562095
  • Davydenko, S. G., Feng, D., Jantti, J., Keranen, S. (2005). "Characterization of GPI14/YJR013w mutation that induces the cell wall integrity signalling pathway and results in increased protein production in Saccharomyces cerevisiae." Yeast 22:993-1009.16134120
  • Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258