Vacuolar amino acid transporter 1 (P47082)

Identification
NameVacuolar amino acid transporter 1
SynonymsNot Available
Gene NameAVT1
Enzyme ClassNot Available
Biological Properties
General FunctionAmino acid transport and metabolism
Specific FunctionRequired for the vacuolar uptake of large neutral amino acids including tyrosine, glutamine, asparagine, isoleucine and leucine. Requires ATP for function
Cellular LocationVacuole membrane; Multi-pass membrane protein
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00002L-GlutamineShow
YMDB00038L-IsoleucineShow
YMDB00226L-AsparagineShow
YMDB00364L-TyrosineShow
YMDB00387L-LeucineShow
YMDB00862hydronShow
GO ClassificationNot Available
Gene Properties
Chromosome Locationchromosome 10
LocusYJR001W
Gene Sequence>1809 bp ATGCCTGAGCAAGAACCATTGAGTCCCAATGGCCGTAAGCGCTCTGAGGTTCACTATATC TCAATCCCATTGAACAGAGGTTCCGCATTCTCACCAGATGATTCTGTATCACAGTTTCAG TCTGACGGGTTCATGACACGTAGGCAATCCATATTGGACCATCCTGTGGGCTCATTTAAG GGAGTTAATTCTTTAAGTCGATTTGCGACTTCGTTGAGGAGAGCCAATTCGTTTCGTAAT ATCGAGCTAAATGCGGATAATGAAAGATCCTTTTTCAAAGAGAGTAACGATGAAACCTAC GATCCGGATACTTTAGCACCAGCTTTGGACGGTAGAAGATTATCAGTAACTTTAAATAAT GCTGGTCGCCCGCGCATTACTAATTTGGCTAACAACGATAGAGTTAGTACAGCCAGCATG GCTATTCACGATGATGATTATGGGTCCATCCAAAATTCAACAATTGGGGATTCTGGGTCA ATATTACGCCCTACTGCCTCCTTAACAGAAATGATGAGTGGTGGAGCGGGGAGAAGGTTT ACAAATAATGACATGGATTCAATTGTAGTGAAGAGAGTGGAGGGTGTAGATGGTAAGGTA GTAACTCTTCTTGCTGGTCAGTCGACCGCTCCACAGACCATATTCAACTCAATCAATGTA CTGATTGGTATTGGGCTCCTGGCGTTACCTCTTGGTTTGAAATACGCCGGCTGGGTTATA GGACTTACTATGCTTGCAATCTTCGCCTTAGCGACATTTTGTACTGCTGAACTTTTGTCT AGGTGCCTAGATACAGATCCAACTTTGATATCTTACGCGGACTTGGGATATGCAGCATTT GGTACAAAAGGGCGCGCACTAATTTCTGCTCTTTTCACACTTGACCTACTGGGTAGTGGG GTGTCCTTGGTGATTCTCTTCGGAGATTCTTTAAATGCTCTATTTCCTCAATACTCCACC ACTTTTTTCAAAATAGTTTCATTTTTTATCGTTACACCCCCGGTTTTTATACCATTGAGT GTTCTATCTAATATCTCGCTTCTGGGAATCCTTTCAACAACCGGAACTGTGCTAGTCATT TGCTGCTGCGGACTATACAAGTCGTCTTCTCCAGGTTCATTAGTTAATCCGATGGAAACG AGCATGTGGCCGATAGATCTAAAGCATCTCTGTTTATCTATAGGTTTATTGAGTGCCTGC TGGGGAGGACATGCGGTTTTTCCCAACTTAAAGACAGATATGAGGCATCCAGATAAATTT AAAGACTGTTTGAAAACTACCTATAAGATAACATCGGTTACGGACATAGGTACTGCAGTT ATTGGCTTTTTGATGTTTGGAAATCTTGTTAAAGATGAAATTACTAAGAACGTTTTGCTG ACGGAGGGATATCCAAAATTCGTTTATGGTCTGATTTCAGCGCTTATGACTATCATTCCT ATCGCAAAGACCCCATTGAACGCAAGACCTATCGTTTCTGTATTGGACGTTCTGATGAAT GTGCAACACATAGATGAAGCGGCCAGCGCCATAAAAAGAAGAGCTGCGAAAGGCCTTCAA GTATTCAATAGGATCTTTATTAATGTTGTCTTCGTTTTGATAGCTATTAATTTCCCTGAA TTTGATAAGATTATCGCATTTCTGGGGGCCGGTTTGTGTTTTACAATTTGCCTAATTTTG CCGTGTTGGTTCTATTTAAGATTGTGCAAGACAACAATCAAACCATGGGAGAGAGTGGCT TGTCACGTAACCATATGTATTAGTGTCGTGTTGTCCACATTAGGTGTTGGTGCTGCAATT ATTTCATAA
Protein Properties
Pfam Domain Function
Protein Residues602
Protein Molecular Weight65345.30078
Protein Theoretical pI8.2
Signalling Regions
  • None
Transmembrane Regions
  • 209-231
  • 241-261
  • 287-307
  • 322-342
  • 345-365
  • 390-410
  • 430-450
  • 467-487
  • 518-538
  • 544-564
  • 581-601
Protein Sequence>Vacuolar amino acid transporter 1 MPEQEPLSPNGRKRSEVHYISIPLNRGSAFSPDDSVSQFQSDGFMTRRQSILDHPVGSFK GVNSLSRFATSLRRANSFRNIELNADNERSFFKESNDETYDPDTLAPALDGRRLSVTLNN AGRPRITNLANNDRVSTASMAIHDDDYGSIQNSTIGDSGSILRPTASLTEMMSGGAGRRF TNNDMDSIVVKRVEGVDGKVVTLLAGQSTAPQTIFNSINVLIGIGLLALPLGLKYAGWVI GLTMLAIFALATFCTAELLSRCLDTDPTLISYADLGYAAFGTKGRALISALFTLDLLGSG VSLVILFGDSLNALFPQYSTTFFKIVSFFIVTPPVFIPLSVLSNISLLGILSTTGTVLVI CCCGLYKSSSPGSLVNPMETSMWPIDLKHLCLSIGLLSACWGGHAVFPNLKTDMRHPDKF KDCLKTTYKITSVTDIGTAVIGFLMFGNLVKDEITKNVLLTEGYPKFVYGLISALMTIIP IAKTPLNARPIVSVLDVLMNVQHIDEAASAIKRRAAKGLQVFNRIFINVVFVLIAINFPE FDKIIAFLGAGLCFTICLILPCWFYLRLCKTTIKPWERVACHVTICISVVLSTLGVGAAI IS
References
External Links
ResourceLink
Saccharomyces Genome Database AVT1
Uniprot IDP47082
Uniprot NameAVT1_YEAST
GenBank Gene IDX87611
Genebank Protein ID854573
General Reference
  • Galibert, F., Alexandraki, D., Baur, A., Boles, E., Chalwatzis, N., Chuat, J. C., Coster, F., Cziepluch, C., De Haan, M., Domdey, H., Durand, P., Entian, K. D., Gatius, M., Goffeau, A., Grivell, L. A., Hennemann, A., Herbert, C. J., Heumann, K., Hilger, F., Hollenberg, C. P., Huang, M. E., Jacq, C., Jauniaux, J. C., Katsoulou, C., Karpfinger-Hartl, L., et, a. l. .. (1996). "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." EMBO J 15:2031-2049.8641269
  • Russnak, R., Konczal, D., McIntire, S. L. (2001). "A family of yeast proteins mediating bidirectional vacuolar amino acid transport." J Biol Chem 276:23849-23857.11274162
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258
  • Li, X., Gerber, S. A., Rudner, A. D., Beausoleil, S. A., Haas, W., Villen, J., Elias, J. E., Gygi, S. P. (2007). "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." J Proteome Res 6:1190-1197.17330950
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956