Dihydrosphingosine 1-phosphate phosphatase LCB3 (P47013)

Identification
NameDihydrosphingosine 1-phosphate phosphatase LCB3
Synonyms
  • Long-chain base protein 3
  • Sphingolipid resistance protein 2
Gene NameLCB3
Enzyme Class
Biological Properties
General FunctionInvolved in catalytic activity
Specific FunctionDihydrosphingosine 1-phosphate phosphatase required for efficient ceramide synthesis from exogenous sphingoid bases. Involved in endocytosis and calcium-mediated signaling
Cellular LocationEndoplasmic reticulum membrane; Multi-pass membrane protein
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG Reactions
Phytosphingosine 1-phosphate + water → Phytosphingosine + phosphate
water + Sphinganine 1-phosphate → Sphinganine + phosphate
Metabolites
YMDB IDNameView
YMDB00285PhytosphingosineShow
YMDB00299Sphinganine 1-phosphateShow
YMDB00327SphinganineShow
YMDB00762Phytosphingosine 1-phosphateShow
YMDB00890waterShow
GO Classification
Component
cell part
membrane
Function
catalytic activity
Process
Not Available
Gene Properties
Chromosome Locationchromosome 10
LocusYJL134W
Gene Sequence>1230 bp ATGGTAGATGGACTGAATACCTCGAACATTAGGAAAAGAGCCAGGACTCTCTCTAACCCC AATGACTTTCAAGAGCCTAATTACTTGCTGGATCCCGGTAATCATCCCTCAGATCATTTT CGAACTCGAATGTCCAAATTTCGGTTTAATATTAGAGAGAAGCTGTTAGTGTTTACCAAC AATCAATCATTCACATTAAGCCGCTGGCAAAAGAAGTACCGTTCTGCGTTTAATGATCTC TACTTTACTTATACTTCCTTAATGGGATCGCATACCTTCTATGTTCTGTGTTTACCTATG CCCGTGTGGTTTGGATATTTTGAAACAACAAAAGATATGGTTTATATCTTGGGATATTCT ATCTACTTGAGTGGTTTTTTTAAAGATTACTGGTGCTTGCCCAGGCCTAGAGCACCTCCA TTACATCGAATTACGTTAAGTGAATATACAACGAAGGAATATGGTGCTCCAAGCTCCCAT ACAGCAAATGCAACAGGAGTGAGTCTCTTGTTTCTCTACAACATCTGGAGGATGCAAGAA TCTTCTGTCATGGTCCAACTATTGTTGTCATGTGTGGTTTTATTTTATTATATGACTTTG GTTTTCGGTAGAATATACTGTGGGATGCATGGCATATTAGATTTAGTAAGCGGTGGGCTC ATTGGAATAGTGTGTTTCATTGTTAGGATGTATTTCAAGTACAGGTTTCCGGGTTTACGC ATTGAGGAGCATTGGTGGTTTCCTTTGTTTAGTGTGGGATGGGGTCTTCTTCTTTTGTTT AAACATGTTAAGCCCGTAGACGAATGTCCTTGCTTCCAAGATAGTGTTGCGTTCATGGGC GTTGTGTCAGGTATTGAATGCTGTGATTGGTTGGGCAAAGTGTTTGGAGTCACCCTGGTG TACAATTTGGAACCTAACTGTGGCTGGCGGTTAACCTTAGCCAGGCTGCTGGTGGGCCTA CCGTGCGTTGTTATCTGGAAGTACGTGATCAGCAAACCGATGATCTACACGTTATTGATC AAAGTTTTCCATCTGAAGGATGACAGAAACGTTGCGGCAAGAAAAAGACTGGAGGCCACG CACAAAGAAGGTGCAAGCAAGTACGAATGTCCATTATATATTGGAGAGCCCAAGATTGAC ATTCTAGGTAGATTTATTATCTATGCTGGCGTTCCATTCACCGTTGTAATGTGCAGCCCC GTCCTATTTTCCCTCTTAAATATAGCATAA
Protein Properties
Pfam Domain Function
Protein Residues409
Protein Molecular Weight47371.30078
Protein Theoretical pI9.13
Signalling Regions
  • None
Transmembrane Regions
  • 87-107
  • 113-133
  • 183-203
  • 208-228
  • 246-266
  • 277-297
  • 316-336
  • 385-405
Protein Sequence>Dihydrosphingosine 1-phosphate phosphatase LCB3 MVDGLNTSNIRKRARTLSNPNDFQEPNYLLDPGNHPSDHFRTRMSKFRFNIREKLLVFTN NQSFTLSRWQKKYRSAFNDLYFTYTSLMGSHTFYVLCLPMPVWFGYFETTKDMVYILGYS IYLSGFFKDYWCLPRPRAPPLHRITLSEYTTKEYGAPSSHTANATGVSLLFLYNIWRMQE SSVMVQLLLSCVVLFYYMTLVFGRIYCGMHGILDLVSGGLIGIVCFIVRMYFKYRFPGLR IEEHWWFPLFSVGWGLLLLFKHVKPVDECPCFQDSVAFMGVVSGIECCDWLGKVFGVTLV YNLEPNCGWRLTLARLLVGLPCVVIWKYVISKPMIYTLLIKVFHLKDDRNVAARKRLEAT HKEGASKYECPLYIGEPKIDILGRFIIYAGVPFTVVMCSPVLFSLLNIA
References
External Links
ResourceLink
Saccharomyces Genome Database LCB3
Uniprot IDP47013
Uniprot NameDS1P1_YEAST
GenBank Gene IDX87371
Genebank Protein ID854562
General Reference
  • Katsoulou, C., Tzermia, M., Tavernarakis, N., Alexandraki, D. (1996). "Sequence analysis of a 40.7 kb segment from the left arm of yeast chromosome X reveals 14 known genes and 13 new open reading frames including homologues of genes clustered on the right arm of chromosome XI." Yeast 12:787-797.8813765
  • Galibert, F., Alexandraki, D., Baur, A., Boles, E., Chalwatzis, N., Chuat, J. C., Coster, F., Cziepluch, C., De Haan, M., Domdey, H., Durand, P., Entian, K. D., Gatius, M., Goffeau, A., Grivell, L. A., Hennemann, A., Herbert, C. J., Heumann, K., Hilger, F., Hollenberg, C. P., Huang, M. E., Jacq, C., Jauniaux, J. C., Katsoulou, C., Karpfinger-Hartl, L., et, a. l. .. (1996). "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." EMBO J 15:2031-2049.8641269
  • Qie, L., Nagiec, M. M., Baltisberger, J. A., Lester, R. L., Dickson, R. C. (1997). "Identification of a Saccharomyces gene, LCB3, necessary for incorporation of exogenous long chain bases into sphingolipids." J Biol Chem 272:16110-16117.9195906
  • Mao, C., Wadleigh, M., Jenkins, G. M., Hannun, Y. A., Obeid, L. M. (1997). "Identification and characterization of Saccharomyces cerevisiae dihydrosphingosine-1-phosphate phosphatase." J Biol Chem 272:28690-28694.9353337
  • Mandala, S. M., Thornton, R., Tu, Z., Kurtz, M. B., Nickels, J., Broach, J., Menzeleev, R., Spiegel, S. (1998). "Sphingoid base 1-phosphate phosphatase: a key regulator of sphingolipid metabolism and stress response." Proc Natl Acad Sci U S A 95:150-155.9419344
  • Mao, C., Saba, J. D., Obeid, L. M. (1999). "The dihydrosphingosine-1-phosphate phosphatases of Saccharomyces cerevisiae are important regulators of cell proliferation and heat stress responses." Biochem J 342 Pt 3:667-675.10477278
  • Zanolari, B., Friant, S., Funato, K., Sutterlin, C., Stevenson, B. J., Riezman, H. (2000). "Sphingoid base synthesis requirement for endocytosis in Saccharomyces cerevisiae." EMBO J 19:2824-2833.10856228
  • Mao, C., Obeid, L. M. (2000). "Yeast sphingosine-1-phosphate phosphatases: assay, expression, deletion, purification, and cellular localization by GFP tagging." Methods Enzymol 311:223-232.10563329
  • Birchwood, C. J., Saba, J. D., Dickson, R. C., Cunningham, K. W. (2001). "Calcium influx and signaling in yeast stimulated by intracellular sphingosine 1-phosphate accumulation." J Biol Chem 276:11712-11718.11278643
  • Ferguson-Yankey, S. R., Skrzypek, M. S., Lester, R. L., Dickson, R. C. (2002). "Mutant analysis reveals complex regulation of sphingolipid long chain base phosphates and long chain bases during heat stress in yeast." Yeast 19:573-586.11967828
  • Kobayashi, S. D., Nagiec, M. M. (2003). "Ceramide/long-chain base phosphate rheostat in Saccharomyces cerevisiae: regulation of ceramide synthesis by Elo3p and Cka2p." Eukaryot Cell 2:284-294.12684378
  • Kihara, A., Sano, T., Iwaki, S., Igarashi, Y. (2003). "Transmembrane topology of sphingoid long-chain base-1-phosphate phosphatase, Lcb3p." Genes Cells 8:525-535.12786943
  • Funato, K., Lombardi, R., Vallee, B., Riezman, H. (2003). "Lcb4p is a key regulator of ceramide synthesis from exogenous long chain sphingoid base in Saccharomyces cerevisiae." J Biol Chem 278:7325-7334.12493772
  • Gruhler, A., Olsen, J. V., Mohammed, S., Mortensen, P., Faergeman, N. J., Mann, M., Jensen, O. N. (2005). "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Mol Cell Proteomics 4:310-327.15665377
  • Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258
  • Li, X., Gerber, S. A., Rudner, A. D., Beausoleil, S. A., Haas, W., Villen, J., Elias, J. E., Gygi, S. P. (2007). "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." J Proteome Res 6:1190-1197.17330950
  • Smolka, M. B., Albuquerque, C. P., Chen, S. H., Zhou, H. (2007). "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Proc Natl Acad Sci U S A 104:10364-10369.17563356
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956