Dolichyl-phosphate-mannose--protein mannosyltransferase 4 (P46971)

Identification
NameDolichyl-phosphate-mannose--protein mannosyltransferase 4
SynonymsNot Available
Gene NamePMT4
Enzyme Class
Biological Properties
General FunctionInvolved in mannosyltransferase activity
Specific FunctionTransfers mannose from Dol-P-mannose to Ser or Thr residues on proteins. Seems to be active on GAS1
Cellular LocationEndoplasmic reticulum membrane; Multi-pass membrane protein
SMPDB PathwaysNot Available
KEGG Pathways
O-Mannosyl glycan biosynthesisec00514 Map00514
SMPDB ReactionsNot Available
KEGG Reactions
dolichyl D-mannosyl phosphate → dolichyl phosphate + hydron + mannan
Metabolites
YMDB IDNameView
YMDB00937dolichyl D-mannosyl phosphateShow
GO Classification
Component
cell part
membrane
Function
catalytic activity
transferase activity
transferase activity, transferring glycosyl groups
transferase activity, transferring hexosyl groups
mannosyltransferase activity
Process
metabolic process
macromolecule metabolic process
macromolecule modification
protein modification process
protein amino acid glycosylation
protein amino acid O-linked glycosylation
Gene Properties
Chromosome Locationchromosome 10
LocusYJR143C
Gene Sequence>2289 bp ATGTCTGTGCCCAAAAAACGTAACCATGGGAAGTTACCACCTTCCACTAAGGACGTAGAC GATCCTTCGTTGAAGTACACGAAGGCCGCGCCTAAATGTGAACAAGTTGCTGAACATTGG CTCTTGCAGCCACTACCGGAACCGGAATCACGTTATAGCTTTTGGGTAACAATTGTTACC TTATTAGCGTTTGCTGCTAGATTTTATAAGATCTGGTATCCAAAAGAAGTTGTTTTTGAT GAGGTACATTTCGGGAAATTTGCATCGTATTACTTAGAAAGGTCTTATTTCTTTGACGTT CATCCCCCTTTTGCTAAGATGATGATTGCCTTCATTGGTTGGTTATGTGGCTATGATGGT TCCTTTAAGTTTGATGAGATTGGGTATTCTTATGAAACTCATCCAGCTCCATATATCGCG TACCGTTCTTTCAACGCGATATTGGGCACATTGACTGTACCAATTATGTTCAACACTTTG AAGGAACTGAATTTCAGGGCTATTACATGTGCGTTTGCATCTCTCTTGGTTGCAATCGAT ACTGCGCATGTTACAGAAACTAGGCTGATTTTACTGGATGCCATCTTGATTATTTCTATT GCTGCTACTATGTATTGTTACGTTCGTTTCTACAAGTGCCAATTGCGTCAACCTTTTACA TGGAGTTGGTATATTTGGTTACACGCTACTGGTTTGTCTTTATCCTTCGTGATTTCCACA AAATATGTTGGTGTTATGACATATTCCGCTATTGGTTTTGCTGCTGTGGTCAACTTATGG CAATTACTGGACATCAAGGCGGGTTTGTCCTTGAGGCAGTTCATGAGACATTTTAGTAAA AGGCTGAATGGTTTAGTTTTGATTCCATTTGTGATTTACTTGTTTTGGTTCTGGGTTCAT TTCACCGTTTTGAATACTTCAGGTCCTGGCGACGCATTTATGTCTGCTGAATTTCAAGAA ACACTTAAGGACTCTCCCTTAAGTGTTGACTCTAAAACCGTCAATTATTTTGATATTATC ACTATCAAACATCAAGATACTGACGCATTCTTACATTCTCACTTGGCACGTTACCCACAA CGGTATGAAGATGGTCGTATCTCATCCGCTGGTCAACAAGTTACTGGTTACACGCATCCT GACTTCAACAATCAATGGGAAGTGTTACCACCCCATGGCAGTGATGTCGGTAAAGGACAA GCTGTTCTATTGAACCAGCATATAAGATTGAGACATGTAGCCACCGATACCTACTTGTTA GCCCATGATGTCGCCTCCCCATTTTACCCTACCAATGAAGAAATCACTACAGTAACTTTA GAAGAAGGTGATGGGGAATTATATCCTGAAACACTTTTTGCATTCCAACCACTGAAAAAA TCAGATGAAGGTCACGTTTTAAAGAGTAAAACGGTTTCTTTCCGTCTTTTCCACGTTGAT ACCTCCGTAGCTTTGTGGACTCACAACGACGAGTTGTTACCGGATTGGGGGTTCCAACAA CAAGAAATTAATGGTAACAAAAAAGTTATTGATCCTTCAAATAACTGGGTTGTTGATGAA ATTGTTAACCTGGACGAGGTTCGGAAGGTATATATTCCGAAGGTTGTTAAGCCATTACCA TTCTTGAAGAAATGGATTGAAACTCAAAAATCTATGTTCGAACATAACAATAAACTATCA TCAGAGCATCCATTTGCCTCTGAACCTTACAGTTGGCCCGGTAGTTTAAGTGGTGTTTCG TTCTGGACCAACGGTGACGAAAAGAAGCAAATATATTTCATTGGTAACATCATTGGGTGG TGGTTCCAAGTCATATCATTGGCTGTTTTTGTTGGCATTATCGTGGCCGATTTAATTACT AGACATCGTGGCTATTATGCCCTAAACAAGATGACCAGAGAAAAGCTGTATGGCCCATTG ATGTTTTTCTTCGTCTCCTGGTGCTGTCATTATTTTCCATTCTTTTTAATGGCGCGTCAA AAGTTTTTGCATCATTACTTACCAGCTCATTTAATCGCGTGCTTATTCTCAGGAGCACTA TGGGAAGTAATTTTCAGTGATTGCAAATCATTGGATTTGGAGAAAGACGAGGATATTTCA GGTGCATCATATGAACGGAACCCTAAGGTCTACGTTAAACCCTATACCGTCTTCTTGGTG TGTGTCTCCTGTGCTGTTGCGTGGTTTTTTGTATACTTTTCACCACTAGTGTATGGAGAT GTCAGCTTGTCACCATCGGAAGTTGTTTCTAGAGAGTGGTTTGACATAGAATTAAATTTC TCCAAGTAA
Protein Properties
Pfam Domain Function
Protein Residues762
Protein Molecular Weight87965.5
Protein Theoretical pI6.93
Signalling Regions
  • None
Transmembrane Regions
  • 54-74
  • 137-157
  • 167-187
  • 190-210
  • 218-238
  • 243-263
  • 284-304
  • 594-614
  • 636-656
  • 717-737
Protein Sequence>Dolichyl-phosphate-mannose--protein mannosyltransferase 4 MSVPKKRNHGKLPPSTKDVDDPSLKYTKAAPKCEQVAEHWLLQPLPEPESRYSFWVTIVT LLAFAARFYKIWYPKEVVFDEVHFGKFASYYLERSYFFDVHPPFAKMMIAFIGWLCGYDG SFKFDEIGYSYETHPAPYIAYRSFNAILGTLTVPIMFNTLKELNFRAITCAFASLLVAID TAHVTETRLILLDAILIISIAATMYCYVRFYKCQLRQPFTWSWYIWLHATGLSLSFVIST KYVGVMTYSAIGFAAVVNLWQLLDIKAGLSLRQFMRHFSKRLNGLVLIPFVIYLFWFWVH FTVLNTSGPGDAFMSAEFQETLKDSPLSVDSKTVNYFDIITIKHQDTDAFLHSHLARYPQ RYEDGRISSAGQQVTGYTHPDFNNQWEVLPPHGSDVGKGQAVLLNQHIRLRHVATDTYLL AHDVASPFYPTNEEITTVTLEEGDGELYPETLFAFQPLKKSDEGHVLKSKTVSFRLFHVD TSVALWTHNDELLPDWGFQQQEINGNKKVIDPSNNWVVDEIVNLDEVRKVYIPKVVKPLP FLKKWIETQKSMFEHNNKLSSEHPFASEPYSWPGSLSGVSFWTNGDEKKQIYFIGNIIGW WFQVISLAVFVGIIVADLITRHRGYYALNKMTREKLYGPLMFFFVSWCCHYFPFFLMARQ KFLHHYLPAHLIACLFSGALWEVIFSDCKSLDLEKDEDISGASYERNPKVYVKPYTVFLV CVSCAVAWFFVYFSPLVYGDVSLSPSEVVSREWFDIELNFSK
References
External Links
ResourceLink
Saccharomyces Genome Database PMT4
Uniprot IDP46971
Uniprot NamePMT4_YEAST
GenBank Gene IDX83798
Genebank Protein ID633654
General Reference
  • Immervoll, T., Gentzsch, M., Tanner, W. (1995). "PMT3 and PMT4, two new members of the protein-O-mannosyltransferase gene family of Saccharomyces cerevisiae." Yeast 11:1345-1351.8585318
  • Galibert, F., Alexandraki, D., Baur, A., Boles, E., Chalwatzis, N., Chuat, J. C., Coster, F., Cziepluch, C., De Haan, M., Domdey, H., Durand, P., Entian, K. D., Gatius, M., Goffeau, A., Grivell, L. A., Hennemann, A., Herbert, C. J., Heumann, K., Hilger, F., Hollenberg, C. P., Huang, M. E., Jacq, C., Jauniaux, J. C., Katsoulou, C., Karpfinger-Hartl, L., et, a. l. .. (1996). "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." EMBO J 15:2031-2049.8641269
  • Gentzsch, M., Tanner, W. (1996). "The PMT gene family: protein O-glycosylation in Saccharomyces cerevisiae is vital." EMBO J 15:5752-5759.8918452
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258
  • Imai, K., Noda, Y., Adachi, H., Yoda, K. (2007). "Peculiar protein-protein interactions of the novel endoplasmic reticulum membrane protein Rcr1 and ubiquitin ligase Rsp5." Biosci Biotechnol Biochem 71:249-252.17213653