1,3-beta-glucan synthase component GSC2 (P40989)

Identification

Name

1,3-beta-glucan synthase component GSC2

Synonyms

1,3-beta-D-glucan-UDP glucosyltransferase
FK506 sensitivity protein 2
Glucan synthase of cerevisiae protein 2

Gene Name

GSC2

Enzyme Class

2.4.1.34

Biological Properties

General Function

Involved in 1,3-beta-D-glucan synthase activity

Specific Function

Alternate catalytic subunit of the 1,3-beta-glucan synthase (GS). Synthesizes 1,3-beta-glucan, a major structural component of the yeast cell wall. Required for spore wall assembly. Negative regulation of activity by SMK1 is important for spore wall deposition. Activity is positively regulated by RHO1

Cellular Location

Membrane; Multi-pass membrane protein

SMPDB Pathways

Starch and sucrose metabolism	PW002481
Stress-activated signalling pathways: cell wall stress test 1	PW007861

KEGG Pathways

Starch and sucrose metabolism

ec00500

SMPDB Reactions

1,3-beta-D-Glucan + Uridine 5'-diphosphate ↔ Uridine diphosphate glucose + 1,3-beta-D-Glucan

KEGG Reactions

Uridine diphosphate glucose → Uridine 5'-diphosphate + (1->3)-beta-D-glucan + hydron

Metabolites

YMDB ID	Name	View
YMDB00212	Uridine diphosphate glucose	Show
YMDB00307	Uridine 5'-diphosphate	Show
YMDB00415	UDP-D-glucose	Show
YMDB00862	hydron	Show
YMDB16314	1,3-beta-D-Glucan	Show

GO Classification

Component
1,3-beta-glucan synthase complex
cell part
membrane
macromolecular complex
protein complex
Function
catalytic activity
transferase activity
transferase activity, transferring glycosyl groups
UDP-glycosyltransferase activity
UDP-glucosyltransferase activity
1,3-beta-glucan synthase activity
Process
beta-glucan metabolic process
1,3-beta-glucan metabolic process
1,3-beta-glucan biosynthetic process
metabolic process
primary metabolic process
carbohydrate metabolic process
polysaccharide metabolic process
glucan metabolic process
cellular glucan metabolic process

Gene Properties

Chromosome Location

chromosome 7

Locus

YGR032W

Gene Sequence

>5688 bp ATGTCCTACAACGATCCAAACTTGAATGGACAGTATTACAGTAACGGTGATGGGACTGGT GACGGTAATTACCCTACGTACCAAGTGACACAGGATCAAAGTGCGTACGATGAGTACGGT CAGCCAATCTATACACAAAACCAACTGGATGATGGTTATTATGATCCAAACGAACAATAC GTTGACGGTACACAATTTCCTCAGGGACAAGATCCTTCACAAGACCAAGGTCCTTATAAT AACGATGCTAGTTACTATAACCAACCCCCCAATATGATGAACCCGTCTTCTCAAGATGGA GAGAACTTCTCAGATTTTAGCAGCTATGGTCCCCCATCCGGCACTTATCCTAACGATCAA TATACTCCTTCTCAAATGAGTTATCCTGATCAAGATGGTTCTTCAGGGACCTCAACCCCC TATGGAAATGGTGTCGTTAATGGTAATGGCCAGTACTACGACCCTAATGCTATTGAAATG GCTTTACCAAATGATCCATATCCCGCATGGACCGCAGATCCCCAGTCTCCCCTGCCCATC GAACAAATCGAAGATATCTTCATAGATTTAACAAATAAATTCGGTTTTCAGAGAGATTCC ATGAGAAATATGTTTGACCATTTTATGACCCTTTTGGACTCTAGATCTTCTAGGATGTCT CCAGAACAGGCCCTTTTATCATTACATGCAGACTACATAGGTGGAGATACGGCCAACTAC AAAAAATGGTACTTTGCCGCTCAACTTGATATGGATGATGAAATTGGTTTCAGGAATATG GAGTTGGGTAAGCTATCAAGAAAGGCAAGAAAGGCTAAGAAGAGAAATAAAAAAGCCATG CAAGAGGCTAGTCCTGAAGACACTGAGGAGACTTTAAATCAAATTGAGGGTGATAACTCA TTAGAAGCTGCGGATTTTAGATGGAAGTCAAAGATGAATCAACTTTCTCCATTTGAAATG GTTCGGCAAATTGCCTTGTTTTTATTATGTTGGGGCGAGGCAAATCAAGTCAGATTTACC CCGGAGTGTCTTTGTTTCATTTATAAATGCGCCTCTGATTACTTAGATTCTGCACAATGT CAACAACGTCCTGATCCCTTGCCTGAAGGTGATTTTTTGAATAGAGTTATTACTCCTCTT TATCGTTTTATTAGGAGCCAGGTTTACGAAATCGTGGATGGTCGATACGTGAAGAGTGAA AAAGATCATAACAAAGTTATTGGGTATGATGATGTCAATCAATTATTCTGGTATCCAGAA GGTATAGCAAAAATTGTCATGGAAGATGGAACCAGGTTGATTGATTTGCCAGCAGAGGAG CGTTATTTGAAATTGGGAGAAATTCCCTGGGATGATGTCTTCTTTAAAACTTACAAAGAA ACACGTTCCTGGTTACATTTAGTTACCAACTTCAATCGTATTTGGATCATGCACATCTCA GTATATTGGATGTATTGTGCTTACAATGCTCCAACTTTTTATACTCACAACTATCAACAA TTGGTCGACAATCAGCCTTTGGCAGCTTATAAATGGGCCACTGCAGCATTAGGTGGTACT GTGGCAAGTTTGATTCAAGTTGCCGCTACTTTGTGCGAGTGGTCATTCGTTCCTAGAAAA TGGGCGGGTGCTCAACATTTGTCCCGTAGATTCTGGTTCTTGTGTGTCATTATGGGTATT AATTTGGGGCCTGTGATATTTGTTTTCGCTTATGATAAGGACACAGTATATTCTACTGCC GCTCATGTCGTTGGAGCAGTTATGTTTTTTGTTGCTGTGGCAACACTTGTTTTCTTTTCC GTAATGCCATTGGGTGGATTATTTACATCGTATATGAAAAAGTCCACAAGAAGTTATGTT GCCTCACAGACCTTCACCGCATCTTTTGCTCCATTGCATGGTTTAGACAGGTGGATGTCT TATTTGGTTTGGGTAACCGTTTTTGCTGCTAAATATGCAGAGTCATATTTTTTTCTAATA CTGTCACTAAGAGATCCAATTAGGATTTTATCTACTACATCAATGAGATGTACTGGTGAA TACTGGTGGGGTAATAAGATTTGTAAGGTCCAGCCAAAGATTGTTTTAGGTTTAATGATT GCGACTGACTTCATTTTGTTCTTTTTGGATACCTACTTGTGGTATATCGTTGTTAACACT GTTTTCTCGGTCGGAAAATCGTTCTATTTGGGTATTTCTATCTTAACTCCATGGAGAAAT ATTTTCACTAGATTGCCAAAAAGAATTTATTCTAAGATCTTGGCTACTACTGATATGGAA ATAAAATATAAACCGAAAGTACTAATTTCTCAGATTTGGAATGCTATCATTATCTCCATG TACAGAGAACATTTATTAGCCATAGACCATGTACAAAAATTGTTATATCATCAGGTTCCG TCCGAAATTGAAGGTAAGAGGACTTTGAGAGCACCAACTTTCTTTGTTTCCCAAGATGAC AATAATTTTGAGACTGAATTTTTCCCTAGAGATTCAGAAGCTGAGCGCCGTATTTCATTT TTTGCCCAATCTCTATCCACTCCAATTCCAGAACCACTACCAGTTGACAACATGCCAACA TTTACTGTATTAACTCCCCATTACGCCGAGAGGATTCTATTATCATTGAGAGAAATTATT CGTGAAGATGATCAATTTTCAAGAGTTACTCTTTTGGAATACCTGAAGCAATTACACCCG GTAGAATGGGACTGTTTTGTTAAGGATACGAAAATTCTTGCTGAAGAAACGGCCGCATAT GAAAACAATGAAGATGAACCTGAAAAGGAAGACGCTCTGAAATCTCAAATTGATGATTTA CCTTTCTATTGTATTGGTTTCAAATCTGCTGCACCAGAATACACCTTACGTACGAGAATC TGGGCCTCTTTAAGGTCGCAAACTTTGTATCGCACAATCTCGGGGTTTATGAATTATTCG AGGGCCATAAAATTACTTTATCGTGTGGAAAATCCAGAAATCGTTCAAATGTTCGGTGGT AATGCTGATGGATTAGAAAGAGAACTGGAAAAAATGGCAAGGCGAAAATTCAAATTCTTG GTTTCGATGCAAAGATTGGCCAAGTTTAAACCACATGAACTAGAAAATGCTGAGTTCCTG TTGAGAGCTTATCCGGACTTGCAAATTGCCTACCTGGATGAAGAACCTCCCTTAAACGAA GGCGAAGAGCCAAGAATTTACTCGGCCTTAATTGATGGTCATTGTGAGATTTTAGAGAAT GGTCGTAGACGTCCCAAATTTAGAGTTCAACTATCCGGTAATCCAATTCTTGGTGATGGT AAATCAGATAATCAAAATCATGCTTTGATTTTTTACAGAGGTGAGTATATTCAATTGATT GATGCTAATCAAGACAATTACTTGGAAGAGTGTTTGAAAATCAGGTCTGTCTTAGCAGAA TTTGAAGAATTGGGAATTGAGCAAATTCATCCTTATACTCCTGGTTTAAAATATGAGGAC CAATCCACAAATCATCCTGTTGCAATTGTCGGCGCTAGAGAATATATTTTCTCAGAAAAC TCTGGTGTTCTTGGTGATGTAGCGGCTGGTAAAGAACAAACTTTTGGTACATTATTTGCC CGTACTTTGGCACAGATTGGTGGTAAATTGCATTATGGTCATCCAGATTTTATTAATGCG ACATTCATGACTACTAGGGGTGGTGTTTCCAAAGCACAAAAGGGTCTACATTTAAATGAA GATATTTATGCCGGTATGAATGCCGTACTTCGGGGTGGTCGTATCAAGCATTGCGAATAT TATCAGTGTGGTAAAGGTAGAGATTTAGGTTTTGGTACAATTTTGAATTTCACTACTAAG ATCGGTGCTGGTATGGGTGAACAAATGTTATCTCGTGAATACTATTATTTGGGTACGCAA TTACCTATTGACCGTTTTTTAACATTTTATTATGCGCATCCAGGGTTTCACTTGAATAAC TTATTTATTCAATTGTCTCTGCAGATGTTCATGTTAACTTTAGTGAACTTGCATGCTTTG GCTCATGAATCCATTCTGTGTGTTTACGATAGGGATAAGCCAATTACTGATGTTTTGTAT CCAATTGGTTGTTACAACTTTCATCCTGCGATTGATTGGGTGAGACGTTATACACTCTCT ATTTTCATCGTCTTTTGGATTGCTTTTGTCCCTATTGTCGTTCAGGAATTAATCGAGCGT GGTCTGTGGAAGGCGACACAAAGATTTTTCCGTCACATTTTATCTCTATCTCCAATGTTT GAAGTCTTTGCTGGCCAAATCTATTCTTCAGCACTGTTAAGTGATATCGCTGTGGGTGGT GCTCGTTATATTTCAACAGGTCGTGGCTTTGCTACATCTCGTATACCTTTCTCTATTCTT TATTCAAGATTTGCGGGTTCAGCCATTTATATGGGATCAAGATCAATGTTGATGTTATTA TTTGGTACCGTGGCACATTGGCAAGCTCCACTATTATGGTTTTGGGCATCATTATCAGCC TTAATCTTTGCACCATTCATTTTCAATCCACATCAATTTGCTTGGGAAGATTTTTTCCTA GACTACAGAGATTATATCAGATGGCTGTCAAGAGGTAATAATAAGTACCACAGGAACTCA TGGATTGGTTATGTAAGAATGTCGAGGTCTCGTGTTACTGGTTTCAAGCGCAAACTGGTG GGTGATGAGTCTGAAAAATCTGCAGGCGATGCAAGCAGGGCTCATAGAACCAATTTAATT ATGGCTGAAATTATACCGTGTGCGATTTACGCAGCAGGTTGTTTTATTGCCTTCACGTTT ATTAATGCACAAACTGGTGTCAAGACTACTGATGAAGATAGAGTAAATTCCACCTTACGT ATCATCATTTGCACCTTGGCGCCTATTGTTATCGATATTGGTGTTTTATTCTTCTGTATG GGTTTGTCATGCTGCTCTGGCCCTTTGTTGGGCATGTGCTGCAAGAAAACTGGTTCTGTT ATGGCAGGGATCGCTCACGGTATCGCTGTTGTTGTCCATATTGTCTTTTTCATTGTCATG TGGGTTTTAGAGGGTTTTAGTTTTGTTAGGATGTTGATTGGCGTTGTTACATGTATACAA TGTCAAAGGTTGATTTTTCACTGTATGACTGTACTGTTGCTGACCCGTGAGTTCAAGAAT GATCACGCTAATACTGCCTTTTGGACAGGCAAATGGTACAGCACCGGTTTAGGATATATG GCATGGACTCAACCGACAAGGGAATTGACTGCAAAAGTCATTGAGCTTTCCGAGTTTGCA GCGGATTTTGTTTTGGGGCATGTAATTTTGATCTTCCAACTACCAGTCATTTGTATTCCA AAGATAGATAAGTTTCACTCCATCATGTTATTTTGGTTAAAACCATCCCGTCAAATCCGT CCTCCTATTTACTCTTTGAAACAAGCACGCCTACGTAAACGTATGGTTAGGAGGTATTGC AGCTTGTACTTTTTGGTACTGATCATATTCGCGGGATGCATCGTTGGCCCTGCCGTTGCT TCAGCACATGTTCCAAAAGACCTTGGATCTGGGTTGACGGGTACTTTCCATAACTTGGTT CAACCAAGGAACGTATCTAACAATGATACAGGGTCCCAGATGTCTACTTATAAGAGTCAT TATTACACTCATACGCCATCCTTAAAGACCTGGTCTACGATCAAATGA

Protein Properties

Pfam Domain Function

Glucan_synthase (PF02364 )

Protein Residues

1895

Protein Molecular Weight

216988.0

Protein Theoretical pI

7.02

Signalling Regions

None

Transmembrane Regions

474-494
512-532
551-571
583-603
1580-1600
1621-1641
1759-1779
1822-1842

Protein Sequence

>1,3-beta-glucan synthase component GSC2 MSYNDPNLNGQYYSNGDGTGDGNYPTYQVTQDQSAYDEYGQPIYTQNQLDDGYYDPNEQY VDGTQFPQGQDPSQDQGPYNNDASYYNQPPNMMNPSSQDGENFSDFSSYGPPSGTYPNDQ YTPSQMSYPDQDGSSGASTPYGNGVVNGNGQYYDPNAIEMALPNDPYPAWTADPQSPLPI EQIEDIFIDLTNKFGFQRDSMRNMFDHFMTLLDSRSSRMSPEQALLSLHADYIGGDTANY KKWYFAAQLDMDDEIGFRNMKLGKLSRKARKAKKKNKKAMQEASPEDTEETLNQIEGDNS LEAADFRWKSKMNQLSPFEMVRQIALFLLCWGEANQVRFTPECLCFIYKCASDYLDSAQC QQRPDPLPEGDFLNRVITPLYRFIRSQVYEIVDGRYVKSEKDHNKVIGYDDVNQLFWYPE GIAKIVMEDGTRLIDLPAEERYLKLGEIPWDDVFFKTYKETRSWLHLVTNFNRIWIMHIS VYWMYCAYNAPTFYTHNYQQLVDNQPLAAYKWATAALGGTVASLIQVAATLCEWSFVPRK WAGAQHLSRRFWFLCVIMGINLGPVIFVFAYDKDTVYSTAAHVVGAVMFFVAVATLVFFS VMPLGGLFTSYMKKSTRSYVASQTFTASFAPLHGLDRWMSYLVWVTVFAAKYAESYFFLI LSLRDPIRILSTTSMRCTGEYWWGNKICKVQPKIVLGLMIATDFILFFLDTYLWYIVVNT VFSVGKSFYLGISILTPWRNIFTRLPKRIYSKILATTDMEIKYKPKVLISQIWNAIIISM YREHLLAIDHVQKLLYHQVPSEIEGKRTLRAPTFFVSQDDNNFETEFFPRDSEAERRISF FAQSLSTPIPEPLPVDNMPTFTVLTPHYAERILLSLREIIREDDQFSRVTLLEYLKQLHP VEWDCFVKDTKILAEETAAYENNEDEPEKEDALKSQIDDLPFYCIGFKSAAPEYTLRTRI WASLRSQTLYRTISGFMNYSRAIKLLYRVENPEIVQMFGGNADGLERELEKMARRKFKFL VSMQRLAKFKPHELENAEFLLRAYPDLQIAYLDEEPPLNEGEEPRIYSALIDGHCEILEN GRRRPKFRVQLSGNPILGDGKSDNQNHALIFYRGEYIQLIDANQDNYLEECLKIRSVLAE FEELGIEQIHPYTPGLKYEDQSTNHPVAIVGAREYIFSENSGVLGDVAAGKEQTFGTLFA RTLAQIGGKLHYGHPDFINATFMTTRGGVSKAQKGLHLNEDIYAGMNAVLRGGRIKHCEY YQCGKGRDLGFGTILNFTTKIGAGMGEQMLSREYYYLGTQLPIDRFLTFYYAHPGFHLNN LFIQLSLQMFMLTLVNLHALAHESILCVYDRDKPITDVLYPIGCYNFHPAIDWVRRYTLS IFIVFWIAFVPIVVQELIERGLWKATQRFFRHILSLSPMFEVFAGQIYSSALLSDIAVGG ARYISTGRGFATSRIPFSILYSRFAGSAIYMGSRSMLMLLFGTVAHWQAPLLWFWASLSA LIFAPFIFNPHQFAWEDFFLDYRDYIRWLSRGNNKYHRNSWIGYVRMSRSRVTGFKRKLV GDESEKSAGDASRAHRTNLIMAEIIPCAIYAAGCFIAFTFINAQTGVKTTDEDRVNSTLR IIICTLAPIVIDIGVLFFCMGLSCCSGPLLGMCCKKTGSVMAGIAHGIAVVVHIVFFIVM WVLEGFSFVRMLIGVVTCIQCQRLIFHCMTVLLLTREFKNDHANTAFWTGKWYSTGLGYM AWTQPTRELTAKVIELSEFAADFVLGHVILIFQLPVICIPKIDKFHSIMLFWLKPSRQIR PPIYSLKQARLRKRMVRRYCSLYFLVLIIFAGCIVGPAVASAHVPKDLGSGLTGTFHNLV QPRNVSNNDTGSQMSTYKSHYYTHTPSLKTWSTIK

References

External Links

Resource	Link
Saccharomyces Genome Database	GSC2
Uniprot ID	P40989
Uniprot Name	FKS2_YEAST
GenBank Gene ID	D42127
Genebank Protein ID	577655

General Reference

Inoue, S. B., Takewaki, N., Takasuka, T., Mio, T., Adachi, M., Fujii, Y., Miyamoto, C., Arisawa, M., Furuichi, Y., Watanabe, T. (1995). "Characterization and gene cloning of 1,3-beta-D-glucan synthase from Saccharomyces cerevisiae." Eur J Biochem 231:845-854.7649185
Mazur, P., Morin, N., Baginsky, W., el-Sherbeini, M., Clemas, J. A., Nielsen, J. B., Foor, F. (1995). "Differential expression and function of two homologous subunits of yeast 1,3-beta-D-glucan synthase." Mol Cell Biol 15:5671-5681.7565718
Deutschbauer, A. M., Davis, R. W. (2005). "Quantitative trait loci mapped to single-nucleotide resolution in yeast." Nat Genet 37:1333-1340.16273108
Rieger, M., Bruckner, M., Schafer, M., Muller-Auer, S. (1997). "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII." Yeast 13:1077-1090.9290212
Tettelin, H., Agostoni Carbone, M. L., Albermann, K., Albers, M., Arroyo, J., Backes, U., Barreiros, T., Bertani, I., Bjourson, A. J., Bruckner, M., Bruschi, C. V., Carignani, G., Castagnoli, L., Cerdan, E., Clemente, M. L., Coblenz, A., Coglievina, M., Coissac, E., Defoor, E., Del Bino, S., Delius, H., Delneri, D., de Wergifosse, P., Dujon, B., Kleine, K., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Nature 387:81-84.9169869
Mazur, P., Baginsky, W. (1996). "In vitro activity of 1,3-beta-D-glucan synthase requires the GTP-binding protein Rho1." J Biol Chem 271:14604-14609.8662910
Peng, J., Schwartz, D., Elias, J. E., Thoreen, C. C., Cheng, D., Marsischky, G., Roelofs, J., Finley, D., Gygi, S. P. (2003). "A proteomics approach to understanding protein ubiquitination." Nat Biotechnol 21:921-926.12872131
Lesage, G., Sdicu, A. M., Menard, P., Shapiro, J., Hussein, S., Bussey, H. (2004). "Analysis of beta-1,3-glucan assembly in Saccharomyces cerevisiae using a synthetic interaction network and altered sensitivity to caspofungin." Genetics 167:35-49.15166135
Huang, L. S., Doherty, H. K., Herskowitz, I. (2005). "The Smk1p MAP kinase negatively regulates Gsc2p, a 1,3-beta-glucan synthase, during spore wall morphogenesis in Saccharomyces cerevisiae." Proc Natl Acad Sci U S A 102:12431-12436.16116083
Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258
Ishihara, S., Hirata, A., Nogami, S., Beauvais, A., Latge, J. P., Ohya, Y. (2007). "Homologous subunits of 1,3-beta-glucan synthase are important for spore wall assembly in Saccharomyces cerevisiae." Eukaryot Cell 6:143-156.17158736
Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956