Identification
NamePhosphatidylinositol 4-kinase PIK1
Synonyms
  • PI4-kinase
  • PtdIns-4-kinase
Gene NamePIK1
Enzyme Class
Biological Properties
General FunctionInvolved in binding
Specific FunctionActs on phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,- trisphosphate. PIK1 is part of a nuclear phosphoinositide cycle and could control cytokinesis through the actin cytoskeleton
Cellular LocationNucleus
SMPDB Pathways
Inositol MetabolismPW002498 ThumbThumb?image type=greyscaleThumb?image type=simple
Inositol phosphate metabolismPW002495 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
Inositol phosphate metabolismec00562 Map00562
SMPDB Reactions
1-Phosphatidyl-D-myo-inositol + Adenosine triphosphate1-Phosphatidyl-1D-myo-inositol 3-phosphate + ADP
1-phosphatidyl-1D-myo-inositol 4,5-biphosphate + Adenosine triphosphatePhosphatidylinositol-3,4,5-trisphosphate + ADP
Phosphatidylinositol-3,4,5-trisphosphate + ADP1-phosphatidyl-1D-myo-inositol 4,5-biphosphate + Adenosine triphosphate
KEGG Reactions
Adenosine triphosphate + 1-Phosphatidyl-D-myo-inositol + hydron ↔ 1-phosphatidyl-1D-myo-inositol 4-phosphate + ADP
Adenosine triphosphate + 1-Phosphatidyl-D-myo-inositol + hydron → 1-phosphatidyl-1D-myo-inositol 4-phosphate + ADP
Metabolites
YMDB IDNameView
YMDB00109Adenosine triphosphateShow
YMDB002301-Phosphatidyl-D-myo-inositolShow
YMDB00862hydronShow
YMDB00914ADPShow
YMDB161651-Phosphatidyl-1D-myo-inositol 3-phosphateShow
YMDB16180Phosphatidylinositol-3,4,5-trisphosphateShow
YMDB162511-phosphatidyl-1D-myo-inositol 4,5-biphosphateShow
GO Classification
Component
Not Available
Function
phosphotransferase activity, alcohol group as acceptor
inositol or phosphatidylinositol kinase activity
catalytic activity
transferase activity
transferase activity, transferring phosphorus-containing groups
binding
kinase activity
Process
biological regulation
regulation of biological process
regulation of cellular process
signal transduction
organophosphate metabolic process
phospholipid metabolic process
intracellular signal transduction
second-messenger-mediated signaling
phosphoinositide-mediated signaling
metabolic process
glycerophospholipid metabolic process
phosphoinositide metabolic process
phosphoinositide phosphorylation
Gene Properties
Chromosome Locationchromosome 14
LocusYNL267W
Gene Sequence>3201 bp ATGCATAAAGCATCCAGTTCAAAGAAAAGCTTTGATGATACTATTGAACTAAAAAAAAAT GAACAATTACTGAAACTGATCAACTCTAGTGAATTCACCCTTCACAATTGTGTGGAGTTA CTGTGCAAACACTCCGAAAATATTGGTATACATTACTATTTATGTCAGAAATTGGCCACA TTTCCTCACAGCGAACTACAGTTCTATATTCCCCAACTAGTACAGGTCCTCGTCACCATG GAGACAGAATCAATGGCTTTAGAAGATTTACTATTAAGGTTGAGGGCGGAAAACCCTCAT TTTGCACTACTGACGTTTTGGCAGTTACAAGCGTTACTAACGGATTTATCTACTGACCCC GCTTCTTATGGCTTCCAAGTGGCTAGAAGAGTCTTAAACAACTTACAAACCAATCTCTTT AACACAAGTTCAGGTAGCGATAAAAATGTTAAAATACATGAAAACGTTGCACCGGCCTTA GTTCTTTCCTCTATGATAATGTCGGCTATAGCATTCCCCCAATTAAGCGAAGTGACCAAA CCATTAGTGGAATCTCAAGGTAGAAGACAAAAAGCTTTCGTTTTTAAGTTGGCTAGAAGT GCAATGAAAGATTTTACCAAGAACATGACCTTGAAAAATACCCTACTAAACAAGAAAACT TCCAGATCCAAAAGAGTTAGCTCAAATCGCAGTTCAACTCCGACTTCTCCAATAGATTTA ATAGATCCAATAAAAACTAAAGAAGATGCATCCTTCAGGAAATCCAGACATAGTGAGGTA AAATTGGATTTCGACATTGTGGATGATATAGGTAACCAGGTCTTTGAAGAAAGAATCTCA TCTTCAATCAAACTCCCAAAACGTAAACCTAAGTATTTGGATAATTCATACGTTCATAGG ACATATGATGGCAAAAATATAAACAGGGACGGAAGCATTTCAAATACCGCAAAGGCTCTC GATGGAAATAAAGGTGATTATATTTCTCCAAAGGGACGTAATGATGAAAATAATGAGATT GGTAACAATGAAGATGAAACTGGTGGTGAAACGGAGGAGGACGCGGACGCTTTAAACTCT GATCACTTCACCAGTTCTATGCCAGATCTGCATAATATTCAACCAAGGACTTCTTCTGCT TCATCTGCTTCTTTAGAAGGGACACCTAAGTTAAACAGGACCAATTCCCAACCCCTTTCG CGCCAGGCATTCAAAAACAGTAAAAAAGCGAATTCTTCTTTGAGTCAAGAAATTGACTTG TCGCAATTATCGACCACTTCAAAAATAAAAATGTTAAAAGCAAATTATTTTCGTTGCGAG ACACAATTTGCCATTGCGCTAGAAACAATATCTCAAAGGCTAGCTCGAGTACCGACAGAA GCTAGATTGAGTGCCTTACGAGCCGAGTTGTTTCTATTAAACAGGGACCTACCAGCGGAA GTAGATATCCCCACTTTATTGCCTCCAAATAAAAAAGGAAAGTTACATAAATTAGTGACC ATTACTGCTAATGAGGCACAAGTTTTGAACTCTGCAGAAAAAGTTCCATATTTACTCCTG ATAGAATACTTGAGAGACGAATTTGATTTTGACCCAACAAGTGAAACCAATGAAAGATTA TTAAAGAAGATCAGTGGTAATCAGGGAGGCTTGATATTTGATTTAAATTACATGAATAGA AAAGAAAACAACGAAAATAGAAATGAAAGTACTCTTACTAGCAACAACACTCGATCTTCG GTATATGATAGTAACTCGTTCAACAACGGGGCCTCCCGCAATGAAGGTCTATCCAGTACC TCAAGAAGTGATTCAGCGTCCACAGCTCATGTTAGGACTGAAGTCAATAAAGAGGAAGAT TTAGGTGATATGTCAATGGTAAAAGTCAGGAACAGAACGGATGACGAAGCGTATAGAAAT GCTTTAGTAATACAGAGTGCCGCAAATGTTCCAATTTTACCTGATGATAGTCAAGACAGA AGCCCAGAGTTGAACTTTGGCTCAAACTTGGACGAAGTACTCATCGAGAATGGAATTAAT AGCAAAAATATACATAGCCAAACTGACGCTTTAGCAGACCAGATGAGAGTTTCAGCTGTT ATGTTAGCACAATTGGATAAGTCGCCACAACAGTTATCAGAAAGTACCAAACAAATCCGC GCTCAAATTATTTCATCAATGAAGGAGGTGCAGGATAAATTTGGTTACCATGATTTGGAA GCCCTTCATGGAATGGCAGGTGAGAGAAAATTGGAAAACGATTTAATGACGGGTGGTATT GACACTTCATATCTAGGTGAAGATTGGGCTACCAAGAAAGAGAGGATACGTAAAACTTCG GAATATGGCCATTTCGAAAACTGGGATTTATGTTCTGTAATCGCCAAGACGGGTGATGAT TTGAGACAGGAGGCGTTTGCATACCAGATGATTCAAGCGATGGCCAATATTTGGGTTAAA GAAAAAGTTGACGTTTGGGTTAAAAGAATGAAAATTTTAATTACTAGTGCGAATACGGGA CTTGTGGAGACCATCACAAATGCTATGTCTGTGCATAGTATTAAAAAGGCTTTAACCAAA AAAATGATTGAAGATGCAGAACTAGATGACAAGGGTGGTATTGCCTCTTTGAATGATCAC TTCCTTAGAGCTTTTGGTAATCCTAATGGATTTAAGTATAGAAGAGCACAAGACAACTTT GCTTCTTCGTTAGCCGCATATTCTGTCATTTGCTATCTCTTGCAGGTTAAAGATAGACAC AACGGTAACATTATGATCGATAACGAAGGCCATGTAAGTCACATCGATTTTGGATTTATG CTATCAAATTCACCCGGCTCAGTGGGCTTTGAGGCCGCACCATTCAAATTAACTTACGAA TATATTGAACTGCTAGGCGGAGTAGAGGGAGAAGCGTTTAAAAAGTTTGTTGAACTAACT AAAAGTTCGTTCAAGGCTCTGAGAAAGTATGCTGATCAAATCGTATCAATGTGTGAGATT ATGCAAAAGGACAATATGCAGCCTTGTTTCGATGCTGGCGAACAAACAAGTGTACAACTA CGACAAAGGTTCCAATTGGACTTATCAGAAAAAGAAGTTGATGACTTCGTAGAAAATTTC TTGATAGGTAAATCTTTGGGTAGTATTTACACCAGAATATACGACCAATTTCAACTTATT ACACAGGGTATATATAGCTGA
Protein Properties
Pfam Domain Function
Protein Residues1066
Protein Molecular Weight119922.0
Protein Theoretical pI6.46
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Phosphatidylinositol 4-kinase PIK1 MHKASSSKKSFDDTIELKKNEQLLKLINSSEFTLHNCVELLCKHSENIGIHYYLCQKLAT FPHSELQFYIPQLVQVLVTMETESMALEDLLLRLRAENPHFALLTFWQLQALLTDLSTDP ASYGFQVARRVLNNLQTNLFNTSSGSDKNVKIHENVAPALVLSSMIMSAIAFPQLSEVTK PLVESQGRRQKAFVFKLARSAMKDFTKNMTLKNTLLNKKTSRSKRVSSNRSSTPTSPIDL IDPIKTKEDASFRKSRHSEVKLDFDIVDDIGNQVFEERISSSIKLPKRKPKYLDNSYVHR TYDGKNINRDGSISNTAKALDGNKGDYISPKGRNDENNEIGNNEDETGGETEEDADALNS DHFTSSMPDLHNIQPRTSSASSASLEGTPKLNRTNSQPLSRQAFKNSKKANSSLSQEIDL SQLSTTSKIKMLKANYFRCETQFAIALETISQRLARVPTEARLSALRAELFLLNRDLPAE VDIPTLLPPNKKGKLHKLVTITANEAQVLNSAEKVPYLLLIEYLRDEFDFDPTSETNERL LKKISGNQGGLIFDLNYMNRKENNENRNESTLTSNNTRSSVYDSNSFNNGASRNEGLSST SRSDSASTAHVRTEVNKEEDLGDMSMVKVRNRTDDEAYRNALVIQSAANVPILPDDSQDR SPELNFGSNLDEVLIENGINSKNIHSQTDALADQMRVSAVMLAQLDKSPQQLSESTKQIR AQIISSMKEVQDKFGYHDLEALHGMAGERKLENDLMTGGIDTSYLGEDWATKKERIRKTS EYGHFENWDLCSVIAKTGDDLRQEAFAYQMIQAMANIWVKEKVDVWVKRMKILITSANTG LVETITNAMSVHSIKKALTKKMIEDAELDDKGGIASLNDHFLRAFGNPNGFKYRRAQDNF ASSLAAYSVICYLLQVKDRHNGNIMIDNEGHVSHIDFGFMLSNSPGSVGFEAAPFKLTYE YIELLGGVEGEAFKKFVELTKSSFKALRKYADQIVSMCEIMQKDNMQPCFDAGEQTSVQL RQRFQLDLSEKEVDDFVENFLIGKSLGSIYTRIYDQFQLITQGIYS
References
External Links
ResourceLink
Saccharomyces Genome Database PIK1
Uniprot IDP39104
Uniprot NamePIK1_YEAST
GenBank Gene IDL20220
Genebank Protein ID432496
General Reference
  • Garcia-Bustos, J. F., Marini, F., Stevenson, I., Frei, C., Hall, M. N. (1994). "PIK1, an essential phosphatidylinositol 4-kinase associated with the yeast nucleus." EMBO J 13:2352-2361.8194527
  • Flanagan, C. A., Schnieders, E. A., Emerick, A. W., Kunisawa, R., Admon, A., Thorner, J. (1993). "Phosphatidylinositol 4-kinase: gene structure and requirement for yeast cell viability." Science 262:1444-1448.8248783
  • Sen-Gupta, M., Lyck, R., Fleig, U., Niedenthal, R. K., Hegemann, J. H. (1996). "The sequence of a 24,152 bp segment from the left arm of chromosome XIV from Saccharomyces cerevisiae between the BNI1 and the POL2 genes." Yeast 12:505-514.8740425
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  • Hendricks, K. B., Wang, B. Q., Schnieders, E. A., Thorner, J. (1999). "Yeast homologue of neuronal frequenin is a regulator of phosphatidylinositol-4-OH kinase." Nat Cell Biol 1:234-241.10559922
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Gruhler, A., Olsen, J. V., Mohammed, S., Mortensen, P., Faergeman, N. J., Mann, M., Jensen, O. N. (2005). "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Mol Cell Proteomics 4:310-327.15665377
  • Li, X., Gerber, S. A., Rudner, A. D., Beausoleil, S. A., Haas, W., Villen, J., Elias, J. E., Gygi, S. P. (2007). "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." J Proteome Res 6:1190-1197.17330950
  • Chi, A., Huttenhower, C., Geer, L. Y., Coon, J. J., Syka, J. E., Bai, D. L., Shabanowitz, J., Burke, D. J., Troyanskaya, O. G., Hunt, D. F. (2007). "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Proc Natl Acad Sci U S A 104:2193-2198.17287358
  • Smolka, M. B., Albuquerque, C. P., Chen, S. H., Zhou, H. (2007). "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Proc Natl Acad Sci U S A 104:10364-10369.17563356
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956
  • Strahl, T., Huttner, I. G., Lusin, J. D., Osawa, M., King, D., Thorner, J., Ames, J. B. (2007). "Structural insights into activation of phosphatidylinositol 4-kinase (Pik1) by yeast frequenin (Frq1)." J Biol Chem 282:30949-30959.17720810