GPI transamidase component GAA1 (P39012)

Identification
NameGPI transamidase component GAA1
SynonymsNot Available
Gene NameGAA1
Enzyme ClassNot Available
Biological Properties
General FunctionInvolved in GPI-anchor transamidase activity
Specific FunctionComponent of the GPI transamidase complex. Required for a terminal step of GPI anchor attachment onto proteins. Affects endocytosis
Cellular LocationEndoplasmic reticulum membrane; Multi-pass membrane protein
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
GO Classification
Component
macromolecular complex
protein complex
GPI-anchor transamidase complex
cell part
membrane part
intrinsic to membrane
integral to membrane
Gene Properties
Chromosome LocationNot Available
Locus
Gene Sequence>1845 bp ATGGCCTTATTGGAGAAGTTGCATCGAAGGATTGTTGATATGGGGCTTGTCCCGCGTATA ATCGCCTTATTACCAGTTATTTCCATGCTATGCGCTCTATTTGGGTTTATTTCTATAGCT ATTCTGCCTATGGATGGACAGTACAGAAGAACATACATTTCTGAGAATGCATTGATGCCT TCACAAGCGTATAGTTACTTTAGAGAATCTGAATGGAACATTTTGAGGGGCTATCGATCT CAAATTAAAGAAATGGTAAACATGACTTCTATGGAAAGAAACAATTTGATGGGTTCTTGG TTACAAGAATTTGGTACTAAGACTGCTATTTACGAAAATGAACAATATGGAGAAACATTG TACGGTGTAATGCACGCTCCTAGGGGTGATGGAACAGAAGCGATGGTGCTTGCCGTTCCA TGGTTTAATTCAGATGATGAATTCAATATTGGCGGCGCAGCTTTGGGTGTATCTTTAGCA AGATTTTTCTCACGTTGGCCAGTATGGTCCAAGAATATAATTGTTGTCTTCAGCGAAAAT CCTCGTGCAGCATTAAGATCATGGGTTGAGGCATACCATACTTCCTTAGATTTGACTGGT GGTTCCATTGAAGCTGCTGTTGTGTTGGATTATTCGAGTACGGAAGATTTCTTCGAGTAT GTAGAAATCTCATACGACGGTCTGAATGGTGAGCTGCCCAATTTGGATCTTGTCAACATC GCTATATCCATTACGGAACATGAAGGTATGAAAGTTTCTTTGCACGGTCTACCCAGTGAT CAGTTAACTAATAATAATTTCTGGTCAAGATTAAAAATATTATGCCTGGGAATAAGGGAT TGGGCGTTGTCCGGTGTTAAAAAGCCCCATGGTAACGAGGCATTTAGCGGCTGGAGGATT CAATCTGTAACATTGAAAGCACATGGAAACAGTGGTCATGATATTACTACATTTGGACGT ATACCCGAAGCAATGTTTCGCTCTATTAATAACCTTTTGGAAAAATTTCACCAATCGTTC TTCTTTTATTTGTTATTAGCACCACGTCAGTTCGTATCCATTAGTAGTTATTTGCCAAGC GCTGTGGCTTTATCTATAGCATTCGCCATAAGTTCATTAAATGCATTTATAAACAATGCT TATGCAAATATATCCTTATTTTCCGAGTATAATTTGGTAGCGTTGTTGGTTTGGTTCGTG TCATTGGTGATATCATTTGTTGTTTCACAAGCGTTTCTTCTAATACCTTCATCGGGATTA TTGATGACAATTAGCATGGCATCTTGTTTTTTACCTTTGATACTTTCCAGAAAAATTCAC ATCTCAGAACCACTATCATACAGGTTGAAAAATGTTGCTTTTTTATATTTCAGTTTGGTT TCAACATCTTTGCTAATGATAAACTTTGCAATGGCTTTACTGATCGGCACATTGGCATTT CCTATGACATTTGTGAAGACCATTGTTGAAAGTTCTAGCGAACATGAGGTGACAACTCAA TCCTCTAACCCAATAAAAACTGAGCCGAAAGATGAGATAGAGCTCGTCGAGAATCACATG GATACAACGCCAGCAACCCCCCAACAACAGAAACAAAAACTAAAAAATTTAGTACTATTA ATTTTGACAAATCCATTTATTTCAATAACCTTATTCGGACTATTTTTTGATGATGAATTT CATGGATTTGATATAATAAACAAACTGGTTTCAGCATGGTTGGATTTGAAATGTTGGAGT TGGTTTGTACTTTGTATAGGTTGGCTTCCATGTTGGCTATTGATATTAGCGTCATCGTTT GAATCTAAATCTGTCGTAGTAAGGTCGAAAGAAAAGCAAAGTTAG
Protein Properties
Pfam Domain Function
Protein Residues614
Protein Molecular Weight69220.5
Protein Theoretical pI6.2
Signalling Regions
  • None
Transmembrane Regions
  • 20-40
  • 357-377
  • 395-415
  • 465-485
  • 536-556
  • 578-598
Protein Sequence>GPI transamidase component GAA1 MALLEKLHRRIVDMGLVPRIIALLPVISMLCALFGFISIAILPMDGQYRRTYISENALMP SQAYSYFRESEWNILRGYRSQIKEMVNMTSMERNNLMGSWLQEFGTKTAIYENEQYGETL YGVMHAPRGDGTEAMVLAVPWFNSDDEFNIGGAALGVSLARFFSRWPVWSKNIIVVFSEN PRAALRSWVEAYHTSLDLTGGSIEAAVVLDYSSTEDFFEYVEISYDGLNGELPNLDLVNI AISITEHEGMKVSLHGLPSDQLTNNNFWSRLKILCLGIRDWALSGVKKPHGNEAFSGWRI QSVTLKAHGNSGHDITTFGRIPEAMFRSINNLLEKFHQSFFFYLLLAPRQFVSISSYLPS AVALSIAFAISSLNAFINNAYANISLFSEYNLVALLVWFVSLVISFVVSQAFLLIPSSGL LMTISMASCFLPLILSRKIHISEPLSYRLKNVAFLYFSLVSTSLLMINFAMALLIGTLAF PMTFVKTIVESSSEHEVTTQSSNPIKTEPKDEIELVENHMDTTPATPQQQKQKLKNLVLL ILTNPFISITLFGLFFDDEFHGFDIINKLVSAWLDLKCWSWFVLCIGWLPCWLLILASSF ESKSVVVRSKEKQS
References
External Links
ResourceLink
Saccharomyces Genome Database GAA1
Uniprot IDP39012
Uniprot NameGAA1_YEAST
GenBank Gene IDU53880
Genebank Protein ID1256890
General Reference
  • Hamburger, D., Egerton, M., Riezman, H. (1995). "Yeast Gaa1p is required for attachment of a completed GPI anchor onto proteins." J Cell Biol 129:629-639.7730400
  • Johnston, M., Hillier, L., Riles, L., Albermann, K., Andre, B., Ansorge, W., Benes, V., Bruckner, M., Delius, H., Dubois, E., Dusterhoft, A., Entian, K. D., Floeth, M., Goffeau, A., Hebling, U., Heumann, K., Heuss-Neitzel, D., Hilbert, H., Hilger, F., Kleine, K., Kotter, P., Louis, E. J., Messenguy, F., Mewes, H. W., Hoheisel, J. D., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Nature 387:87-90.9169871
  • Fraering, P., Imhof, I., Meyer, U., Strub, J. M., van Dorsselaer, A., Vionnet, C., Conzelmann, A. (2001). "The GPI transamidase complex of Saccharomyces cerevisiae contains Gaa1p, Gpi8p, and Gpi16p." Mol Biol Cell 12:3295-3306.11598210
  • Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258