RNA polymerase II-associated protein 1 (P38351)

Identification
NameRNA polymerase II-associated protein 1
Synonyms
  • Protein PAF1
Gene NamePAF1
Enzyme ClassNot Available
Biological Properties
General Functiontranscription from RNA polymerase I promoter
Specific FunctionThe PAF1 complex is a multifunctional complex. Involved in transcription initiation via genetic interactions with TATA-binding proteins. Involved in elongation. It regulates 3'-end formation of snR47 by modulating the recruitment or stable association of NRD1 and NAB3 with RNA polymerase II. Also has a role in transcription-coupled histone modification. Required for activation of the RAD6/UBC2-BRE1 ubiquitin ligase complex, which ubiquitinates histone H2B to form H2BK123ub1. Also required for the methylation of histone H3 by the COMPASS complex to form H3K4me, by SET2 to form H3K36me, and by DOT1 to form H3K79me. RNA polymerase II associated protein important for transcription of a subset of genes. Required for both positive and negative regulation. Negatively regulates MAK16 expression. Also required for efficient CLN2 transcription in late G1 and may be involved in transcription of galactose-inducible genes.
Cellular LocationNot Available
SMPDB Pathways
Stress-activated signalling pathways: cell wall stress test 1PW007861 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
GO Classification
Function
DNA-templated transcription, termination
rRNA processing
global genome nucleotide-excision repair
snoRNA 3'-end processing
histone modification
snoRNA transcription from an RNA polymerase II promoter
mRNA 3'-end processing
transcription elongation from RNA polymerase I promoter
negative regulation of DNA recombination
transcription elongation from RNA polymerase II promoter
nucleus
positive regulation of histone H3-K36 trimethylation
transcription from RNA polymerase I promoter
RNA polymerase II core binding
positive regulation of phosphorylation of RNA polymerase II C-terminal domain serine 2 residues
negative regulation of transcription from RNA polymerase II promoter
positive regulation of transcription elongation from RNA polymerase I promoter
chromatin binding
regulation of chromatin silencing at telomere
positive regulation of transcription elongation from RNA polymerase II promoter
regulation of histone H2B conserved C-terminal lysine ubiquitination
Cdc73/Paf1 complex
regulation of histone H2B ubiquitination
transcriptionally active chromatin
regulation of histone H3-K4 methylation
RNA polymerase II C-terminal domain phosphoserine binding
transcription factor activity, RNA polymerase II core promoter sequence-specific
regulation of phosphorylation of RNA polymerase II C-terminal domain serine 2 residues
transcription factor activity, RNA polymerase II transcription factor binding
regulation of transcription from RNA polymerase II promoter
transcription factor activity, TFIIF-class transcription factor binding
regulation of transcription involved in G1/S transition of mitotic cell cycle
chromatin silencing at rDNA
regulation of transcription-coupled nucleotide-excision repair
Gene Properties
Chromosome LocationNot Available
Locus
Gene Sequence>1338 bp ATGTCCAAAAAACAGGAATATATTGCACCAATAAAATACCAAAACAGCTTGCCTGTGCCA CAATTACCTCCTAAACTTCTGGTTTACCCCGAGTCTCCGGAGACGAATGCAGACTCCTCC CAACTAATCAATTCACTATACATCAAAACAAACGTCACTAATCTAATTCAACAGGATGAA GATTTAGGTATGCCGGTTGATTTGATGAAATTCCCAGGCCTATTGAACAAATTAGATTCT AAACTGCTTTACGGCTTTGATAATGTGAAATTGGACAAAGATGATCGAATTTTACTGAGG GACCCTAGAATAGATAGACTGACCAAGACTGATATATCAAAGGTTACCTTCTTGAGACGC ACCGAATATGTCTCCAATACAATTGCAGCCCATGATAACACATCGTTGAAAAGGAAAAGG CGCTTGGATGATGGAGATTCGGATGATGAAAACCTTGATGTTAATCATATAATTAGTAGG GTCGAAGGAACATTCAATAAGACGGACAAATGGCAACATCCGGTGAAGAAGGGTGTCAAG ATGGTTAAAAAATGGGACCTATTGCCCGACACAGCTTCAATGGACCAGGTGTATTTTATC CTAAAATTCATGGGCAGTGCATCATTGGATACTAAAGAAAAAAAATCTTTAAATACAGGA ATTTTCAGGCCAGTGGAACTGGAAGAAGACGAATGGATTTCCATGTATGCCACAGATCAT AAGGACTCTGCCATTCTGGAAAACGAATTGGAAAAGGGTATGGATGAAATGGATGATGAC TCTCATGAAGGTAAAATATACAAGTTTAAAAGGATAAGAGACTACGACATGAAACAAGTG GCTGAAAAGCCAATGACAGAGTTAGCTATACGTTTGAATGATAAGGACGGCATAGCTTAC TACAAACCCCTACGTTCAAAGATCGAATTAAGACGTAGGAGAGTAAACGACATCATTAAA CCTTTGGTGAAGGAGCACGATATAGACCAACTTAATGTTACTTTGAGAAACCCTAGCACT AAGGAAGCTAACATAAGGGACAAGTTAAGAATGAAGTTTGACCCCATAAATTTCGCCACT GTAGATGAAGAAGACGATGAAGACGAGGAGCAACCAGAAGACGTTAAGAAAGAATCGGAA GGCGATTCAAAAACAGAAGGTTCTGAGCAAGAAGGAGAAAATGAGAAGGATGAAGAAATC AAACAGGAAAAAGAGAATGAACAAGATGAAGAAAATAAACAAGATGAAAACCGTGCAGCT GATACGCCCGAAACTTCAGATGCTGTTCATACTGAACAAAAACCAGAGGAAGAAAAGGAA ACTTTACAAGAAGAATAG
Protein Properties
Pfam Domain FunctionNot Available
Protein Residues445
Protein Molecular Weight51800.4
Protein Theoretical pINot Available
Signalling RegionsNot Available
Transmembrane RegionsNot Available
Protein Sequence>RNA polymerase II-associated protein 1 MSKKQEYIAPIKYQNSLPVPQLPPKLLVYPESPETNADSSQLINSLYIKTNVTNLIQQDE DLGMPVDLMKFPGLLNKLDSKLLYGFDNVKLDKDDRILLRDPRIDRLTKTDISKVTFLRR TEYVSNTIAAHDNTSLKRKRRLDDGDSDDENLDVNHIISRVEGTFNKTDKWQHPVKKGVK MVKKWDLLPDTASMDQVYFILKFMGSASLDTKEKKSLNTGIFRPVELEEDEWISMYATDH KDSAILENELEKGMDEMDDDSHEGKIYKFKRIRDYDMKQVAEKPMTELAIRLNDKDGIAY YKPLRSKIELRRRRVNDIIKPLVKEHDIDQLNVTLRNPSTKEANIRDKLRMKFDPINFAT VDEEDDEDEEQPEDVKKESEGDSKTEGSEQEGENEKDEEIKQEKENEQDEENKQDENRAA DTPETSDAVHTEQKPEEEKETLQEE
References
External Links
ResourceLink
Saccharomyces Genome Database PAF1
Uniprot IDP38351
Uniprot NamePAF1
General ReferenceNot Available