Identification
NameGPI mannosyltransferase 2
Synonyms
  • GPI mannosyltransferase II
  • GPI-MT-II
  • Glycosylphosphatidylinositol-anchor biosynthesis protein 18
Gene NameGPI18
Enzyme Class
Biological Properties
General FunctionInvolved in transferase activity, transferring hexosyl groups
Specific FunctionMannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the second mannose to the glycosylphosphatidylinositol during GPI precursor assembly
Cellular LocationEndoplasmic reticulum membrane; Multi-pass membrane protein
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG Reactions
6-O-{2-O-[(2-aminoethyl)phosphoryl]-alpha-D-mannosyl-(1->4)-alpha-D-glucosaminyl}-O-acyl-1-phosphatidyl-1D-myo-inositol + dolichyl D-mannosyl phosphatedolichyl phosphate + 6-O-{alpha-D-mannosyl-(1->6)-2-O-[(2-aminoethyl)phosphoryl]-alpha-D-mannosyl-(1->4)-alpha-D-glucosaminyl}-O-acyl-1-phosphatidyl-1D-myo-inositol
Metabolites
YMDB IDNameView
YMDB00937dolichyl D-mannosyl phosphateShow
YMDB00941dolichyl phosphateShow
GO Classification
Component
cell part
membrane
membrane part
intrinsic to membrane
integral to membrane
organelle membrane
endoplasmic reticulum membrane
Function
catalytic activity
transferase activity
transferase activity, transferring glycosyl groups
transferase activity, transferring hexosyl groups
Process
protein amino acid lipidation
GPI anchor biosynthetic process
metabolic process
macromolecule metabolic process
macromolecule modification
protein modification process
Gene Properties
Chromosome Locationchromosome 2
LocusYBR004C
Gene Sequence>1302 bp ATGATTGTGGGGTTGACACTTTATTTTGTATTATTCCGTTCAATACAGTATTTACTGGTT TTTTTGACTCCAATTAGGCAGTTTGATACATCAACATCACTTTTATTAAACGAATTATGT TCTTCTCCCTCTGAAATCAACAGTTATTGGAACAAGTATTTTTGGAATAAGCTACTATCA TGGGACAGTGTTTTTTTTATCAAGAACATAACTTCCAAAAACGGAAAACCTCAATTTGAG CATGAATACGCGTTTTCTCAGTTGTGGACTTTTTTCGTTAGGCTGTTTATTAAAAGTAAT AACGATAGCATCTACCATGCCTTAAGGGTTGGAGTTGCAATAGAAAATGTTTTATTTTAC TTGTCAGGTATTGTTTTATATTTTCTAACAAAAAAAATTTTCAGCCAAAATATAAGGCAA TCACAGTTTGCTAGAACTATCGCTAAAAAAACATCTCTGTTGTTTTTCTTAACGAGTGCC GCTGGATTTTTAACAAGCATATATTCTGAACCATTATCTTTTTTTTTTGCATTTGTTGGT ATTTGGAGTCGTGAATGCACCATTTCCGTGCCCGTATTGGGTCAATTCGATATTTCGTGG AGATATTGGTTTCCTTACTCCTTTATCAGCATGGCTTGCTTTACCTTAGCATCCTTGAAT CGTTCAAACTGTGTTTTGTTAGGGATTTACTTTATTTTTGACCTTATTGAACTAACAAAG AACAGGAAGTTTGTAAAAGCAATATGTTTCCCACTATTATCAGGATCATTAATGTTTTCT GCTCTACTATATCAACAATATTACCTACCATATAAGACATTTTGTCCTCAAAGGGGTGAA TGGTGTAAATCTCAATTGTTTTCAAGCATTTTTATCACGAAAACTTCTTTATATTCCTAC ATTCAGAGTCATTATTGGGGAGTTGGGTTATTGAAATACTGGACCCCAAACAACATCCCA AACTTTTTGTTTGCTGTCCCAAATATTATTATTTTAATCTATTCCTCCATATATTTCAGC AAAATTTATCCCTCCTATAACTTGAAGGCTCTCGTATGGATCACCAGAGCACTGGTCGTC ATAGTATGCTTTTTTGCCCATGTCCAAATTCTGAATCGTATAGCCTCCTTTTTACCCTTG CACCTTTGGTATTTGGCTGATAGATTGGTTAAAACTTCTGATCCAAAAAAAATGGAAAAT CCGAAAGGTGACGATAAGATAGTCAAGTTTTACATATACTGGTTGGCATTCTGGATACCT TTACAGACTATCCTATTTGCAGCTTTTTTACCACCAGCCTGA
Protein Properties
Pfam Domain FunctionNot Available
Protein Residues433
Protein Molecular Weight50772.19922
Protein Theoretical pI9.81
Signalling Regions
  • None
Transmembrane Regions
  • 2-22
  • 110-130
  • 151-168
  • 170-182
  • 198-220
  • 222-236
  • 244-264
  • 319-339
  • 351-371
  • 410-430
Protein Sequence>GPI mannosyltransferase 2 MIVGLTLYFVLFRSIQYLLVFLTPIRQFDTSTSLLLNELCSSPSEINSYWNKYFWNKLLS WDSVFFIKNITSKNGKPQFEHEYAFSQLWTFFVRLFIKSNNDSIYHALRVGVAIENVLFY LSGIVLYFLTKKIFSQNIRQSQFARTIAKKTSLLFFLTSAAGFLTSIYSEPLSFFFAFVG IWSRECTISVPVLGQFDISWRYWFPYSFISMACFTLASLNRSNCVLLGIYFIFDLIELTK NRKFVKAICFPLLSGSLMFSALLYQQYYLPYKTFCPQRGEWCKSQLFSSIFITKTSLYSY IQSHYWGVGLLKYWTPNNIPNFLFAVPNIIILIYSSIYFSKIYPSYNLKALVWITRALVV IVCFFAHVQILNRIASFLPLHLWYLADRLVKTSDPKKMENPKGDDKIVKFYIYWLAFWIP LQTILFAAFLPPA
References
External Links
ResourceLink
Saccharomyces Genome Database GPI18
Uniprot IDP38211
Uniprot NameGPI18_YEAST
GenBank Gene IDZ35873
Genebank Protein ID536192
General Reference
  • Feldmann, H., Aigle, M., Aljinovic, G., Andre, B., Baclet, M. C., Barthe, C., Baur, A., Becam, A. M., Biteau, N., Boles, E., Brandt, T., Brendel, M., Bruckner, M., Bussereau, F., Christiansen, C., Contreras, R., Crouzet, M., Cziepluch, C., Demolis, N., Delaveau, T., Doignon, F., Domdey, H., Dusterhus, S., Dubois, E., Dujon, B., El Bakkoury, M., Entian, K. D., Feurmann, M., Fiers, W., Fobo, G. M., Fritz, C., Gassenhuber, H., Glandsdorff, N., Goffeau, A., Grivell, L. A., de Haan, M., Hein, C., Herbert, C. J., Hollenberg, C. P., Holmstrom, K., Jacq, C., Jacquet, M., Jauniaux, J. C., Jonniaux, J. L., Kallesoe, T., Kiesau, P., Kirchrath, L., Kotter, P., Korol, S., Liebl, S., Logghe, M., Lohan, A. J., Louis, E. J., Li, Z. Y., Maat, M. J., Mallet, L., Mannhaupt, G., Messenguy, F., Miosga, T., Molemans, F., Muller, S., Nasr, F., Obermaier, B., Perea, J., Pierard, A., Piravandi, E., Pohl, F. M., Pohl, T. M., Potier, S., Proft, M., Purnelle, B., Ramezani Rad, M., Rieger, M., Rose, M., Schaaff-Gerstenschlager, I., Scherens, B., Schwarzlose, C., Skala, J., Slonimski, P. P., Smits, P. H., Souciet, J. L., Steensma, H. Y., Stucka, R., Urrestarazu, A., van der Aart, Q. J., van Dyck, L., Vassarotti, A., Vetter, I., Vierendeels, F., Vissers, S., Wagner, G., de Wergifosse, P., Wolfe, K. H., Zagulski, M., Zimmermann, F. K., Mewes, H. W., Kleine, K. (1994). "Complete DNA sequence of yeast chromosome II." EMBO J 13:5795-5809.7813418
  • Fabre, A. L., Orlean, P., Taron, C. H. (2005). "Saccharomyces cerevisiae Ybr004c and its human homologue are required for addition of the second mannose during glycosylphosphatidylinositol precursor assembly." FEBS J 272:1160-1168.15720390
  • Kang, J. Y., Hong, Y., Ashida, H., Shishioh, N., Murakami, Y., Morita, Y. S., Maeda, Y., Kinoshita, T. (2005). "PIG-V involved in transferring the second mannose in glycosylphosphatidylinositol." J Biol Chem 280:9489-9497.15623507