Identification |
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Name | 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase 1 |
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Synonyms | - Phosphoinositide phospholipase C
- Phospholipase C-1
- PLC-1
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Gene Name | PLC1 |
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Enzyme Class | |
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Biological Properties |
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General Function | Involved in protein binding |
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Specific Function | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Required for cell growth, osmoresistance and expression of GPD1 |
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Cellular Location | Cytoplasmic |
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SMPDB Pathways | |
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KEGG Pathways | Inositol phosphate metabolism | ec00562 | |
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SMPDB Reactions | |
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KEGG Reactions | |
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Metabolites | YMDB ID | Name | View |
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YMDB00291 | Inositol 1,4,5-trisphosphate | Show | YMDB00890 | water | Show | YMDB16247 | ()-Glycerol 1,2-diacetate | Show | YMDB16251 | 1-phosphatidyl-1D-myo-inositol 4,5-biphosphate | Show |
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GO Classification | Component |
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Not Available | Function |
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phospholipase activity | ion binding | phospholipase C activity | cation binding | phosphoric diester hydrolase activity | metal ion binding | phosphoinositide phospholipase C activity | hydrolase activity | calcium ion binding | hydrolase activity, acting on ester bonds | phosphoric ester hydrolase activity | catalytic activity | binding | lipase activity | Process |
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biological regulation | regulation of biological process | regulation of cellular process | signal transduction | signaling | metabolic process | signaling pathway | intracellular signaling pathway | lipid metabolic process | primary metabolic process |
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Gene Properties |
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Chromosome Location | chromosome 16 |
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Locus | YPL268W |
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Gene Sequence | >2610 bp
ATGACTGAAAGTGCTATAGATGACCAAAGGTTTAATCTTACAAAGGAGTTACAACGACAC
TCATGCAGAGATCAAGGGAAAATAACACAAAAGGATGATGCTTTAGATTTTATAAGTTAC
AGTTCTTTTCAATCCTCTTTTAATACTGACCAAAAAAGCGCGAACAATGGAAGTACTGTG
AGAAGAAGCATAAGGTCGATTTTTAGAAGAGCAGCCGAATTGCCTAGAGTCCATATGGGG
CCTCTTACTTATTCACATGGGATAAATGAGCTTGTTAACAAGAAATTAAGAAAAGACTGT
GATCTCAGCACGCTATGTCGCGTATTGCAAAGAGGAATCAGGATGATTAGGATGACAAGA
AGAAGAAGGAAGTTCTATGAATTTAAATTAATCAATAACAACGGGCAAATAATATGGAAA
GATGGTTCGAAGTATCTGGAATTAGACTCCGTTAAAGACATCAGAATCGGTGATACGGCG
AGTACTTATCAAGAGGAAGTAGATCCGAAAAGATTGAGATCGGACAGTAAACTTTGGATT
GCTATTATTTATAAGGTTTCCAACAAATTGAAAGCGTTACACGTAGTTGCTTTGAATGAA
TTGGACTTTAACACATTCTTAAGCTGCATTTGCGGTTTAGTGAAGTTAAGGAGGGAATTA
ATGGAAAGCATACTTTTGCCTGATAATTCGCAATTCGCTAGGATACATTGGCAAATTACT
GTTTCTGAAAAAGAGGAAGATGAGAAAAAGGACACATTGAGCTTTGCAGATGTGAAGAAA
CTATGTGATAAATTTCATATTTATGTGTCAACTGGTCAATTGTTAGAGTTTTTCCAATTA
GCAGACATCAATCATAACGGACTATTAAATTATTTTGAATTTGAAAAGTTCATTAAAATC
TTAAAGAATAGGAAAGAGGTAAATATGATTTGGTCTAAATTTACAAAACCTCCACACTCC
CACTTATCATTTGAAAATTTTTTTCAATTTTTAATTACAGAGCAGCATGAGCAAGTGGAT
CGTCAAACTGCATGGTCATATTTTATTAAGTACAGAGAACCGACTCAGCTGACGATGGGG
CAAGATGGTTTTACCAAGTTCTTGAAGGAGCAACCATATTTGGTGGAGGTTAAAGAGGAG
TTATATTCAAAGCCATTAAACCATTATTTTATTGCATCTTCACATAATACTTATTTATTG
GGGAAACAAATTGCAGAAACTCCATCTGTGGAGGGATATATTCAAGTCCTACAACAAGGT
TGTAGGTGCGTAGAAATTGATATATGGGATGGTGAAAATGGGCCAGTGGTATGCCATGGA
TTTTTAACGTCAGCTATTCCCTTAAAAACAGTTATACGCGTGATAAAGAAGTACGCTTTT
ATAACGTCTCCGTATCCTTTGATTATTTCACTGGAAATAAACTGTAACAAGGATAATCAA
AAATTGGCAAGCCTGATAATGAGAGAGGTTTTAGCAGAACAGCTGTACTTTGTTGGCACA
AGAACTGATAAACTGCCATCACCAAGGGAATTAAAACATAAAATACTGTTGAAATCCAAG
AAGACGAGTGAAGCAACTAGAGGTCTTAGCGTTAATGAGCCATTTCCATCTTCTTTTAGT
TCTTCTTACGAATCAGCTAATGAACAGGAATTAAGAATGAAAGATGACTCCACGAACAGT
AGTAGCGCAACAAATTCTTCTAGTATGCAACGCATCAAAAGAATTGGATTAAAAAAGCAC
GCTGATATAATTAATGATGTTTCTAACATTTCTGGAATACATGGCATCAAGTTCAGGAAT
TTCTCTTTACCAGAGTCCAAAACGATAGCGCATTGTTTTTCACTGAATGAGCGCAAAGTA
GAATACATGATAAAAGACAAACACTTAAAATTATCGTTGGATAAACATAATAGAAGATAT
TTGATGAGAGTCTATCCTCACGTTTTACGATACAAATCATCAAATTTTAACCCAATACCT
TTTTGGAAGGCCGGTGTACAAATGGTGGCTACGAATTGGCAAACTAATGATATTGGACAG
CAACTTAATCTGGCAATGTTTCAAATATTAGATCACCAACCTGATGGAAGTTTTAAATCA
GGATACGTGCTAAAACCAAAAAAGTTGCTACCTGTAGTAACAAAGGCGAAAATGATTCCA
TTAATTTACGAACATTTTGAAAATGGTAGTGATCCTGTTACTGTTAAAATTCGAATTTTA
TCTACACAGTTATTGCCCAGATTGAATGATACATCGCCAAGCAGAAATAACACCAATTCC
TTTGTTAAAGTGGAGTTTCATACCGATGACGAGCCGACAATGCCCATCTCAATCGATAAG
GGTACAAGGATTTCTGCCACTGAAGCATCCACTAAGAGTTCTCAAGGGAATGGATTCAAC
CCAATATGGGATGCTGAAGTTTCTATCACATTGAAAGATACAGACCTCACATTTATAAAA
TTCATGGTTATCTCTGAAGAGACCCAGATTGCATCTGTTTGCCTTAAGCTGAACTATTTG
AGGATGGGATATCGTCACATTCCGTTGTTTAACATGGAGGGGGAACAATACATATTTTGC
ACTTTATTTATTCATACACAAATTTTATAG |
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Protein Properties |
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Pfam Domain Function | |
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Protein Residues | 869 |
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Protein Molecular Weight | 100547.0 |
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Protein Theoretical pI | 9.55 |
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Signalling Regions | |
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Transmembrane Regions | |
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Protein Sequence | >1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase 1
MTESAIDDQRFNLTKELQRHSCRDQGKITQKDDALDFISYSSFQSSFNTDQKSANNGSTV
RRSIRSIFRRAAELPRVHMGPLTYSHGINELVNKKLRKDCDLSTLCRVLQRGIRMIRMTR
RRRKFYEFKLINNNGQIIWKDGSKYLELDSVKDIRIGDTASTYQEEVDPKRLRSDSKLWI
AIIYKVSNKLKALHVVALNELDFNTFLSCICGLVKLRRELMESILLPDNSQFARIHWQIT
VSEKEEDEKKDTLSFADVKKLCDKFHIYVSTGQLLEFFQLADINHNGLLNYFEFEKFIKI
LKNRKEVNMIWSKFTKPPHSHLSFENFFQFLITEQHEQVDRQTAWSYFIKYREPTQLTMG
QDGFTKFLKEQPYLVEVKEELYSKPLNHYFIASSHNTYLLGKQIAETPSVEGYIQVLQQG
CRCVEIDIWDGENGPVVCHGFLTSAIPLKTVIRVIKKYAFITSPYPLIISLEINCNKDNQ
KLASLIMREVLAEQLYFVGTRTDKLPSPRELKHKILLKSKKTSEATRGLSVNEPFPSSFS
SSYESANEQELRMKDDSTNSSSATNSSSMQRIKRIGLKKHADIINDVSNISGIHGIKFRN
FSLPESKTIAHCFSLNERKVEYMIKDKHLKLSLDKHNRRYLMRVYPHVLRYKSSNFNPIP
FWKAGVQMVATNWQTNDIGQQLNLAMFQILDHQPDGSFKSGYVLKPKKLLPVVTKAKMIP
LIYEHFENGSDPVTVKIRILSTQLLPRLNDTSPSRNNTNSFVKVEFHTDDEPTMPISIDK
GTRISATEASTKSSQGNGFNPIWDAEVSITLKDTDLTFIKFMVISEETQIASVCLKLNYL
RMGYRHIPLFNMEGEQYIFCTLFIHTQIL |
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References |
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External Links | |
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General Reference | - Yoko-o, T., Matsui, Y., Yagisawa, H., Nojima, H., Uno, I., Toh-e, A. (1993). "The putative phosphoinositide-specific phospholipase C gene, PLC1, of the yeast Saccharomyces cerevisiae is important for cell growth." Proc Natl Acad Sci U S A 90:1804-1808.8383328
- Flick, J. S., Thorner, J. (1993). "Genetic and biochemical characterization of a phosphatidylinositol-specific phospholipase C in Saccharomyces cerevisiae." Mol Cell Biol 13:5861-5876.8395015
- Payne, W. E., Fitzgerald-Hayes, M. (1993). "A mutation in PLC1, a candidate phosphoinositide-specific phospholipase C gene from Saccharomyces cerevisiae, causes aberrant mitotic chromosome segregation." Mol Cell Biol 13:4351-4364.8391635
- Bussey, H., Storms, R. K., Ahmed, A., Albermann, K., Allen, E., Ansorge, W., Araujo, R., Aparicio, A., Barrell, B., Badcock, K., Benes, V., Botstein, D., Bowman, S., Bruckner, M., Carpenter, J., Cherry, J. M., Chung, E., Churcher, C., Coster, F., Davis, K., Davis, R. W., Dietrich, F. S., Delius, H., DiPaolo, T., Hani, J., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Nature 387:103-105.9169875
- Lin, H., Nguyen, P., Vancura, A. (2002). "Phospholipase C interacts with Sgd1p and is required for expression of GPD1 and osmoresistance in Saccharomyces cerevisiae." Mol Genet Genomics 267:313-320.12073033
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