| Identification |
|---|
| Name | Dolichyl-phosphate-mannose--protein mannosyltransferase 2 |
|---|
| Synonyms | Not Available |
|---|
| Gene Name | PMT2 |
|---|
| Enzyme Class | |
|---|
| Biological Properties |
|---|
| General Function | Involved in mannosyltransferase activity |
|---|
| Specific Function | Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins. Seems to be active on chitinase |
|---|
| Cellular Location | Endoplasmic reticulum membrane; Multi-pass membrane protein |
|---|
| SMPDB Pathways | Not Available |
|---|
| KEGG Pathways | | O-Mannosyl glycan biosynthesis | ec00514 |  |
|
|---|
| SMPDB Reactions | Not Available |
|---|
| KEGG Reactions | Not Available |
|---|
| Metabolites | | YMDB ID | Name | View |
|---|
| YMDB00937 | dolichyl D-mannosyl phosphate | Show |
|
|---|
| GO Classification | | Component |
|---|
| cell part | | membrane | | Function |
|---|
| transferase activity, transferring hexosyl groups | | mannosyltransferase activity | | catalytic activity | | transferase activity | | transferase activity, transferring glycosyl groups | | Process |
|---|
| metabolic process | | macromolecule metabolic process | | macromolecule modification | | protein modification process | | protein amino acid glycosylation | | protein amino acid O-linked glycosylation |
|
|---|
| Gene Properties |
|---|
| Chromosome Location | chromosome 1 |
|---|
| Locus | YAL023C |
|---|
| Gene Sequence | >2280 bp
ATGTCCTCGTCTTCGTCTACCGGGTACAGCAAAAACAATGCCGCCCACATTAAGCAAGAG
AATACACTGAGACAAAGAGAATCGTCTTCCATCAGCGTCAGTGAGGAACTTTCGAGCGCT
GATGAGAGAGACGCGGAAGATTTCTCGAAGGAAAAGCCCGCTGCACAAAGCTCACTGTTA
CGCCTGGAATCCGTTGTAATGCCGGTGATCTTTACTGCATTGGCGTTGTTTACCAGGATG
TACAAAATCGGCATCAACAACCATGTTGTTTGGGATGAGGCGCACTTTGGTAAATTTGGT
TCTTATTACTTGAGACACGAATTTTACCACGATGTCCATCCTCCCCTAGGAAAAATGCTG
GTCGGGTTGTCTGGTTATTTGGCAGGCTACAACGGTTCTTGGGACTTCCCTTCTGGGGAA
ATTTACCCAGACTATTTGGATTATGTTAAAATGAGACTGTTCAACGCGTCATTTTCCGCG
CTCTGTGTGCCATTGGCCTACTTCACTGCCAAAGCTATTGGATTTTCTTTACCAACAGTT
TGGCTGATGACCGTGTTGGTTTTGTTTGAAAACTCGTATAGTACTTTGGGCAGGTTCATT
CTTTTGGACTCCATGCTACTTTTCTTCACTGTCGCATCGTTCTTTAGTTTTGTTATGTTC
CACAACCAGAGGTCCAAGCCGTTCTCTAGAAAGTGGTGGAAATGGCTGTTGATCACTGGT
ATTTCTTTGGGTTGCACTATTTCCGTCAAAATGGTGGGTCTATTTATCATCACTATGGTC
GGTATCTATACTGTGATTGACTTATGGACCTTTTTGGCAGATAAATCCATGTCATGGAAA
ACCTATATTAACCACTGGTTGGCAAGAATATTTGGTCTTATTATCGTCCCCTTCTGCATT
TTCCTATTGTGCTTCAAAATACATTTTGACCTATTATCGCATTCTGGTACAGGTGATGCT
AACATGCCATCTCTTTTCCAAGCAAGATTAGTGGGTTCTGACGTCGGACAAGGCCCCCGT
GACATTGCTCTAGGTTCCTCCGTTGTTTCCATCAAAAACCAAGCTCTTGGAGGATCTCTA
TTGCACTCACATATACAAACTTATCCAGATGGGTCCAACCAACAACAAGTAACCTGTTAT
GGTTACAAAGATGCTAACAACGAATGGTTTTTCAACAGAGAAAGAGGCTTACCATCATGG
TCAGAAAACGAAACTGACATCGAGTATTTGAAGCCAGGTACCTCCTATAGATTGGTACAC
AAAAGCACGGGCAGAAACTTGCACACCCACCCAGTTGCTGCACCAGTGTCAAAGACACAA
TGGGAGGTTTCTGGTTACGGTGACAATGTTGTTGGTGACAACAAAGACAATTGGGTTATT
GAGATCATGGACCAAAGAGGAGATGAAGACCCTGAGAAGTTGCACACATTGACCACCTCT
TTCCGTATCAAGAACTTGGAGATGGGCTGTTACTTGGCTCAAACCGGTAACAGTTTGCCC
GAATGGGGTTTCAGACAACAAGAGGTTGTCTGCATGAAAAACCCATTCAAGAGGGACAAG
AGGACCTGGTGGAACATCGAGACCCACGAAAATGAAAGGTTGCCACCAAGACCCGAAGAT
TTTCAATACCCAAAGACCAACTTCTTAAAAGACTTCATTCATTTAAATCTAGCCATGATG
GCCACTAATAACGCTTTGGTGCCAGATCCAGACAAATTTGATTACTTAGCTTCCTCAGCA
TGGCAATGGCCAACTTTGAATGTGGGTTTGAGACTATGTGGCTGGGGTGATGATAATCCA
AAATACTTCCTATTGGGTACCCCAGCTTCCACGTGGGCTTCTAGTGTTGCCGTCCTCGCA
TTCATGGCCACGGTCGTTATCTTACTGATCAGATGGCAAAGACAATATGTGGACCTAAGA
AATCCATCTAACTGGAACGTTTTCTTAATGGGCGGGTTCTACCCACTACTAGCTTGGGGC
CTACACTACATGCCATTCGTTATCATGTCTAGAGTCACCTACGTTCATCATTACTTGCCT
GCCTTGTATTTTGCACTGATCATTTTGGCGTACTGTTTCGACGCCGGTTTGCAAAAATGG
TCCAGATCTAAGTGCGGCCGTATCATGCGGTTCGTCCTATACGCCGGATTCATGGCACTT
GTAATTGGTTGCTTCTGGTACTTCTCCCCAATATCATTTGGTATGGAGGGACCAAGTAGT
AACTTCCGCTACTTAAACTGGTTTTCCACTTGGGACATTGCCGACAAGCAAGAAGCATGA
|
|---|
| Protein Properties |
|---|
| Pfam Domain Function | |
|---|
| Protein Residues | 759 |
|---|
| Protein Molecular Weight | 86869.0 |
|---|
| Protein Theoretical pI | 7.78 |
|---|
| Signalling Regions | |
|---|
| Transmembrane Regions | - 65-85
- 168-188
- 199-219
- 252-272
- 290-310
- 610-630
- 648-668
- 677-697
- 711-731
|
|---|
| Protein Sequence | >Dolichyl-phosphate-mannose--protein mannosyltransferase 2
MSSSSSTGYSKNNAAHIKQENTLRQRESSSISVSEELSSADERDAEDFSKEKPAAQSSLL
RLESVVMPVIFTALALFTRMYKIGINNHVVWDEAHFGKFGSYYLRHEFYHDVHPPLGKML
VGLSGYLAGYNGSWDFPSGEIYPDYLDYVKMRLFNASFSALCVPLAYFTAKAIGFSLPTV
WLMTVLVLFENSYSTLGRFILLDSMLLFFTVASFFSFVMFHNQRSKPFSRKWWKWLLITG
ISLGCTISVKMVGLFIITMVGIYTVIDLWTFLADKSMSWKTYINHWLARIFGLIIVPFCI
FLLCFKIHFDLLSHSGTGDANMPSLFQARLVGSDVGQGPRDIALGSSVVSIKNQALGGSL
LHSHIQTYPDGSNQQQVTCYGYKDANNEWFFNRERGLPSWSENETDIEYLKPGTSYRLVH
KSTGRNLHTHPVAAPVSKTQWEVSGYGDNVVGDNKDNWVIEIMDQRGDEDPEKLHTLTTS
FRIKNLEMGCYLAQTGNSLPEWGFRQQEVVCMKNPFKRDKRTWWNIETHENERLPPRPED
FQYPKTNFLKDFIHLNLAMMATNNALVPDPDKFDYLASSAWQWPTLNVGLRLCGWGDDNP
KYFLLGTPASTWASSVAVLAFMATVVILLIRWQRQYVDLRNPSNWNVFLMGGFYPLLAWG
LHYMPFVIMSRVTYVHHYLPALYFALIILAYCFDAGLQKWSRSKCGRIMRFVLYAGFMAL
VIGCFWYFSPISFGMEGPSSNFRYLNWFSTWDIADKQEA |
|---|
| References |
|---|
| External Links | |
|---|
| General Reference | - Ouellette, B. F., Clark, M. W., Keng, T., Storms, R. K., Zhong, W., Zeng, B., Fortin, N., Delaney, S., Barton, A., Kaback, D. B., et, a. l. .. (1993). "Sequencing of chromosome I from Saccharomyces cerevisiae: analysis of a 32 kb region between the LTE1 and SPO7 genes." Genome 36:32-42.8458570
- Bussey, H., Kaback, D. B., Zhong, W., Vo, D. T., Clark, M. W., Fortin, N., Hall, J., Ouellette, B. F., Keng, T., Barton, A. B., et, a. l. .. (1995). "The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Proc Natl Acad Sci U S A 92:3809-3813.7731988
- Lussier, M., Gentzsch, M., Sdicu, A. M., Bussey, H., Tanner, W. (1995). "Protein O-glycosylation in yeast. The PMT2 gene specifies a second protein O-mannosyltransferase that functions in addition to the PMT1-encoded activity." J Biol Chem 270:2770-2775.7852348
- Gentzsch, M., Tanner, W. (1996). "The PMT gene family: protein O-glycosylation in Saccharomyces cerevisiae is vital." EMBO J 15:5752-5759.8918452
- Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
- Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258
- Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956
|
|---|