Dolichyl-phosphate-mannose--protein mannosyltransferase 2 (P31382)

Identification
NameDolichyl-phosphate-mannose--protein mannosyltransferase 2
SynonymsNot Available
Gene NamePMT2
Enzyme Class
Biological Properties
General FunctionInvolved in mannosyltransferase activity
Specific FunctionTransfers mannose from Dol-P-mannose to Ser or Thr residues on proteins. Seems to be active on chitinase
Cellular LocationEndoplasmic reticulum membrane; Multi-pass membrane protein
SMPDB PathwaysNot Available
KEGG Pathways
O-Mannosyl glycan biosynthesisec00514 Map00514
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00937dolichyl D-mannosyl phosphateShow
GO Classification
Component
cell part
membrane
Function
catalytic activity
transferase activity
transferase activity, transferring glycosyl groups
transferase activity, transferring hexosyl groups
mannosyltransferase activity
Process
metabolic process
macromolecule metabolic process
macromolecule modification
protein modification process
protein amino acid glycosylation
protein amino acid O-linked glycosylation
Gene Properties
Chromosome Locationchromosome 1
LocusYAL023C
Gene Sequence>2280 bp ATGTCCTCGTCTTCGTCTACCGGGTACAGCAAAAACAATGCCGCCCACATTAAGCAAGAG AATACACTGAGACAAAGAGAATCGTCTTCCATCAGCGTCAGTGAGGAACTTTCGAGCGCT GATGAGAGAGACGCGGAAGATTTCTCGAAGGAAAAGCCCGCTGCACAAAGCTCACTGTTA CGCCTGGAATCCGTTGTAATGCCGGTGATCTTTACTGCATTGGCGTTGTTTACCAGGATG TACAAAATCGGCATCAACAACCATGTTGTTTGGGATGAGGCGCACTTTGGTAAATTTGGT TCTTATTACTTGAGACACGAATTTTACCACGATGTCCATCCTCCCCTAGGAAAAATGCTG GTCGGGTTGTCTGGTTATTTGGCAGGCTACAACGGTTCTTGGGACTTCCCTTCTGGGGAA ATTTACCCAGACTATTTGGATTATGTTAAAATGAGACTGTTCAACGCGTCATTTTCCGCG CTCTGTGTGCCATTGGCCTACTTCACTGCCAAAGCTATTGGATTTTCTTTACCAACAGTT TGGCTGATGACCGTGTTGGTTTTGTTTGAAAACTCGTATAGTACTTTGGGCAGGTTCATT CTTTTGGACTCCATGCTACTTTTCTTCACTGTCGCATCGTTCTTTAGTTTTGTTATGTTC CACAACCAGAGGTCCAAGCCGTTCTCTAGAAAGTGGTGGAAATGGCTGTTGATCACTGGT ATTTCTTTGGGTTGCACTATTTCCGTCAAAATGGTGGGTCTATTTATCATCACTATGGTC GGTATCTATACTGTGATTGACTTATGGACCTTTTTGGCAGATAAATCCATGTCATGGAAA ACCTATATTAACCACTGGTTGGCAAGAATATTTGGTCTTATTATCGTCCCCTTCTGCATT TTCCTATTGTGCTTCAAAATACATTTTGACCTATTATCGCATTCTGGTACAGGTGATGCT AACATGCCATCTCTTTTCCAAGCAAGATTAGTGGGTTCTGACGTCGGACAAGGCCCCCGT GACATTGCTCTAGGTTCCTCCGTTGTTTCCATCAAAAACCAAGCTCTTGGAGGATCTCTA TTGCACTCACATATACAAACTTATCCAGATGGGTCCAACCAACAACAAGTAACCTGTTAT GGTTACAAAGATGCTAACAACGAATGGTTTTTCAACAGAGAAAGAGGCTTACCATCATGG TCAGAAAACGAAACTGACATCGAGTATTTGAAGCCAGGTACCTCCTATAGATTGGTACAC AAAAGCACGGGCAGAAACTTGCACACCCACCCAGTTGCTGCACCAGTGTCAAAGACACAA TGGGAGGTTTCTGGTTACGGTGACAATGTTGTTGGTGACAACAAAGACAATTGGGTTATT GAGATCATGGACCAAAGAGGAGATGAAGACCCTGAGAAGTTGCACACATTGACCACCTCT TTCCGTATCAAGAACTTGGAGATGGGCTGTTACTTGGCTCAAACCGGTAACAGTTTGCCC GAATGGGGTTTCAGACAACAAGAGGTTGTCTGCATGAAAAACCCATTCAAGAGGGACAAG AGGACCTGGTGGAACATCGAGACCCACGAAAATGAAAGGTTGCCACCAAGACCCGAAGAT TTTCAATACCCAAAGACCAACTTCTTAAAAGACTTCATTCATTTAAATCTAGCCATGATG GCCACTAATAACGCTTTGGTGCCAGATCCAGACAAATTTGATTACTTAGCTTCCTCAGCA TGGCAATGGCCAACTTTGAATGTGGGTTTGAGACTATGTGGCTGGGGTGATGATAATCCA AAATACTTCCTATTGGGTACCCCAGCTTCCACGTGGGCTTCTAGTGTTGCCGTCCTCGCA TTCATGGCCACGGTCGTTATCTTACTGATCAGATGGCAAAGACAATATGTGGACCTAAGA AATCCATCTAACTGGAACGTTTTCTTAATGGGCGGGTTCTACCCACTACTAGCTTGGGGC CTACACTACATGCCATTCGTTATCATGTCTAGAGTCACCTACGTTCATCATTACTTGCCT GCCTTGTATTTTGCACTGATCATTTTGGCGTACTGTTTCGACGCCGGTTTGCAAAAATGG TCCAGATCTAAGTGCGGCCGTATCATGCGGTTCGTCCTATACGCCGGATTCATGGCACTT GTAATTGGTTGCTTCTGGTACTTCTCCCCAATATCATTTGGTATGGAGGGACCAAGTAGT AACTTCCGCTACTTAAACTGGTTTTCCACTTGGGACATTGCCGACAAGCAAGAAGCATGA
Protein Properties
Pfam Domain Function
Protein Residues759
Protein Molecular Weight86869.0
Protein Theoretical pI7.78
Signalling Regions
  • None
Transmembrane Regions
  • 65-85
  • 168-188
  • 199-219
  • 252-272
  • 290-310
  • 610-630
  • 648-668
  • 677-697
  • 711-731
Protein Sequence>Dolichyl-phosphate-mannose--protein mannosyltransferase 2 MSSSSSTGYSKNNAAHIKQENTLRQRESSSISVSEELSSADERDAEDFSKEKPAAQSSLL RLESVVMPVIFTALALFTRMYKIGINNHVVWDEAHFGKFGSYYLRHEFYHDVHPPLGKML VGLSGYLAGYNGSWDFPSGEIYPDYLDYVKMRLFNASFSALCVPLAYFTAKAIGFSLPTV WLMTVLVLFENSYSTLGRFILLDSMLLFFTVASFFSFVMFHNQRSKPFSRKWWKWLLITG ISLGCTISVKMVGLFIITMVGIYTVIDLWTFLADKSMSWKTYINHWLARIFGLIIVPFCI FLLCFKIHFDLLSHSGTGDANMPSLFQARLVGSDVGQGPRDIALGSSVVSIKNQALGGSL LHSHIQTYPDGSNQQQVTCYGYKDANNEWFFNRERGLPSWSENETDIEYLKPGTSYRLVH KSTGRNLHTHPVAAPVSKTQWEVSGYGDNVVGDNKDNWVIEIMDQRGDEDPEKLHTLTTS FRIKNLEMGCYLAQTGNSLPEWGFRQQEVVCMKNPFKRDKRTWWNIETHENERLPPRPED FQYPKTNFLKDFIHLNLAMMATNNALVPDPDKFDYLASSAWQWPTLNVGLRLCGWGDDNP KYFLLGTPASTWASSVAVLAFMATVVILLIRWQRQYVDLRNPSNWNVFLMGGFYPLLAWG LHYMPFVIMSRVTYVHHYLPALYFALIILAYCFDAGLQKWSRSKCGRIMRFVLYAGFMAL VIGCFWYFSPISFGMEGPSSNFRYLNWFSTWDIADKQEA
References
External Links
ResourceLink
Saccharomyces Genome Database PMT2
Uniprot IDP31382
Uniprot NamePMT2_YEAST
GenBank Gene IDL05146
Genebank Protein ID27316977
General Reference
  • Ouellette, B. F., Clark, M. W., Keng, T., Storms, R. K., Zhong, W., Zeng, B., Fortin, N., Delaney, S., Barton, A., Kaback, D. B., et, a. l. .. (1993). "Sequencing of chromosome I from Saccharomyces cerevisiae: analysis of a 32 kb region between the LTE1 and SPO7 genes." Genome 36:32-42.8458570
  • Bussey, H., Kaback, D. B., Zhong, W., Vo, D. T., Clark, M. W., Fortin, N., Hall, J., Ouellette, B. F., Keng, T., Barton, A. B., et, a. l. .. (1995). "The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Proc Natl Acad Sci U S A 92:3809-3813.7731988
  • Lussier, M., Gentzsch, M., Sdicu, A. M., Bussey, H., Tanner, W. (1995). "Protein O-glycosylation in yeast. The PMT2 gene specifies a second protein O-mannosyltransferase that functions in addition to the PMT1-encoded activity." J Biol Chem 270:2770-2775.7852348
  • Gentzsch, M., Tanner, W. (1996). "The PMT gene family: protein O-glycosylation in Saccharomyces cerevisiae is vital." EMBO J 15:5752-5759.8918452
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956