| Identification |
|---|
| Name | Putative lipase ATG15 |
|---|
| Synonyms | - Autophagy-related protein 15
- Cytoplasm to vacuole targeting protein 17
|
|---|
| Gene Name | ATG15 |
|---|
| Enzyme Class | |
|---|
| Biological Properties |
|---|
| General Function | Involved in triglyceride lipase activity |
|---|
| Specific Function | Essential for lysis of subvacuolar cytoplasm to vacuole targeted bodies and intravacuolar autophagic bodies. Involved in the lysis of intravacuolar multivesicular body (MVB) vesicles |
|---|
| Cellular Location | Endoplasmic reticulum membrane; Single-pass type II membrane protein. Golgi apparatus membrane; Single-pass type II membrane protein. Endosome, multivesicular body membrane; Single-pass type II membrane protein. Prevacuolar compartment membrane; Single-pa |
|---|
| SMPDB Pathways | Not Available |
|---|
| KEGG Pathways | |
|---|
| SMPDB Reactions | Not Available |
|---|
| KEGG Reactions | Not Available |
|---|
| Metabolites | | YMDB ID | Name | View |
|---|
| YMDB00069 | Palmitic acid | Show | | YMDB00679 | myristic acid | Show | | YMDB00809 | TG(16:0/16:0/16:0) | Show | | YMDB00825 | Trimyristin | Show | | YMDB00834 | 1,3-Dipalmitin | Show | | YMDB00890 | water | Show |
|
|---|
| GO Classification | | Component |
|---|
| Not Available | | Function |
|---|
| hydrolase activity | | hydrolase activity, acting on ester bonds | | carboxylesterase activity | | triglyceride lipase activity | | catalytic activity | | Process |
|---|
| primary metabolic process | | lipid metabolic process | | metabolic process |
|
|---|
| Gene Properties |
|---|
| Chromosome Location | chromosome 3 |
|---|
| Locus | YCR068W |
|---|
| Gene Sequence | >1563 bp
ATGTTGCATAAAAGCCCTTCAAGAAAGAGATTTGCTTCTCCTTTGCATCTAGGATGCATT
CTAACGCTTACAGTGCTCTGCCTTATTGCTTACTACTTTGCTTTACCGGATTATTTATCG
GTAGGGAAGAGCTCATCCAGGGGTGCCATGGATCAAAAATCTGATGGAACCTTTAGACTT
AAGTCTATCTACAGGCATGGCGTCGGAGCAAACCATCGGCTACACCAGAGGCTGGAGGTC
ACTCCAGAAGTTATTTCTGCCGCTGGAATGCTGTATCAAGAAACCACAACTCAAGGACAG
GATTTCGAAGACCAAGAACCCCTATGGACTACTAATGCCGAATATGCCACCACTAACCCA
TTCGATTTTGAGTTTGAGCTACGAAGGATGCCGCTATTGATGAAGCGGATGAAGGAAAGA
GATCCTGAATTCATAGAGTCGTACATTTACGGAGAAACATACATGACGGAGGAGGAAGAA
CACGCCATGTGGATAGATGATGACATTGTGGCACCCAATATCACAGATAGAGGTACAGTA
GTGTCGTTAGCATTAATGTCGTCTAATGCTTACGTGAGAATACCGCAAACGGGAGACTGG
CGTAACGTCACGGAGCCGTGGAATGAAACAGAGCCGGAAGATTTTGGTTGGGATGGTGAT
GGTATACGTGGTCACGTCTTTTACAATGAAGTGGAGAATATCGTGGTACTCTCGATAAAG
GGAACCAGCGCTCAGGGTTTGCCAGGGTCTGGCGAAGATGAGACAACTGGAAACGACAAG
ATCAACGATAATTTGTTATTTTCGTGTTGTTGTGCAAGAGTGAGCTACCTGTGGACTACC
GTGTGCGATTGCTATGTGAAGTCGTATATATGCGATGAGTCTTGCCTGGAAAAGGAACTG
AGACGTAAGGATAGATTTTACTCTGCAGTCGTTGACATATACAAAGGCGTCCTCAAAGAA
TACCCCGATGCGGCCATATGGGTCACAGGCCACTCACTGGGAGGCGCATTGGCCAGTTTA
CTGGGCCGCACTTTTGGATTGCCTGCGGTCGCATTCGAGTCCCCTGGAGAGCTACTACCT
TCAAAAAGACTTCACTTACCATTCCCACCGGGACTTCCCTCATACATGGAGGGTATCTGG
CACTTCGGTCACAACGCAGACCCAATCTTCATGGGTACATGCAACGGAGCTAGTTCAAGT
TGCTCACTGGTGGGCTACGCTATGGAAACCGCGTGCCACACTGGTAGAGTCTGTGTCTAC
GATGTGGTCAACGACAAGGGCTGGAGTGTAAACATGTTCAACCACAGAATCCACAAAGTC
ATTGACGAAGTTCTCCTTGGATACGAGCAGGCTGCCAAGTGCGTTGAACCAGAGCCCTGC
GTAGATTGCTACAACTGGAAGTTTATTCCAAGCAGAGACTGGGAATCCTCATCGAGGCTC
ATTACCAAGACTAAAAGCCACGCTGCACCAACCACGACGACTCGCACCACTGCTACTACT
ACCTCTTCATCTACCTGTGTAGGCCGCAATTGGCTTGGCTTCTGCACCAAATACGAGTTG
TAA |
|---|
| Protein Properties |
|---|
| Pfam Domain Function | |
|---|
| Protein Residues | 520 |
|---|
| Protein Molecular Weight | 58434.5 |
|---|
| Protein Theoretical pI | 5.25 |
|---|
| Signalling Regions | |
|---|
| Transmembrane Regions | |
|---|
| Protein Sequence | >Putative lipase ATG15
MLHKSPSRKRFASPLHLGCILTLTVLCLIAYYFALPDYLSVGKSSSRGAMDQKSDGTFRL
KSIYRHGVGANHRLHQRLEVTPEVISAAGMLYQETTTQGQDFEDQEPLWTTNAEYATTNP
FDFEFELRRMPLLMKRMKERDPEFIESYIYGETYMTEEEEHAMWIDDDIVAPNITDRGTV
VSLALMSSNAYVRIPQTGDWRNVTEPWNETEPEDFGWDGDGIRGHVFYNEVENIVVLSIK
GTSAQGLPGSGEDETTGNDKINDNLLFSCCCARVSYLWTTVCDCYVKSYICDESCLEKEL
RRKDRFYSAVVDIYKGVLKEYPDAAIWVTGHSLGGALASLLGRTFGLPAVAFESPGELLP
SKRLHLPFPPGLPSYMEGIWHFGHNADPIFMGTCNGASSSCSLVGYAMETACHTGRVCVY
DVVNDKGWSVNMFNHRIHKVIDEVLLGYEQAAKCVEPEPCVDCYNWKFIPSRDWESSSRL
ITKTKSHAAPTTTTRTTATTTSSSTCVGRNWLGFCTKYEL |
|---|
| References |
|---|
| External Links | |
|---|
| General Reference | - Teter, S. A., Eggerton, K. P., Scott, S. V., Kim, J., Fischer, A. M., Klionsky, D. J. (2001). "Degradation of lipid vesicles in the yeast vacuole requires function of Cvt17, a putative lipase." J Biol Chem 276:2083-2087.11085977
- Oliver, S. G., van der Aart, Q. J., Agostoni-Carbone, M. L., Aigle, M., Alberghina, L., Alexandraki, D., Antoine, G., Anwar, R., Ballesta, J. P., Benit, P., et, a. l. .. (1992). "The complete DNA sequence of yeast chromosome III." Nature 357:38-46.1574125
- Epple, U. D., Suriapranata, I., Eskelinen, E. L., Thumm, M. (2001). "Aut5/Cvt17p, a putative lipase essential for disintegration of autophagic bodies inside the vacuole." J Bacteriol 183:5942-5955.11566994
- Epple, U. D., Eskelinen, E. L., Thumm, M. (2003). "Intravacuolar membrane lysis in Saccharomyces cerevisiae. Does vacuolar targeting of Cvt17/Aut5p affect its function?" J Biol Chem 278:7810-7821.12499386
- Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
|
|---|
