Inorganic phosphate transporter PHO84 (P25297)

Identification
NameInorganic phosphate transporter PHO84
SynonymsNot Available
Gene NamePHO84
Enzyme ClassNot Available
Biological Properties
General FunctionInvolved in inorganic phosphate transmembrane transporter activity
Specific FunctionHigh-affinity transporter for external inorganic phosphate. Is not an essential protein, since a constitutive, low affinity pI transporter exists in yeast
Cellular LocationMembrane; Multi-pass membrane protein
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00862hydronShow
GO Classification
Component
cell part
membrane
membrane part
intrinsic to membrane
integral to membrane
Function
inorganic anion transmembrane transporter activity
phosphate transmembrane transporter activity
inorganic phosphate transmembrane transporter activity
transporter activity
transmembrane transporter activity
substrate-specific transmembrane transporter activity
ion transmembrane transporter activity
anion transmembrane transporter activity
Process
ion transport
anion transport
inorganic anion transport
phosphate transport
transmembrane transport
establishment of localization
transport
Gene Properties
Chromosome Locationchromosome 13
LocusYML123C
Gene Sequence>1764 bp ATGAGTTCCGTCAATAAAGATACTATTCATGTTGCTGAAAGAAGTCTTCATAAAGAACAC CTTACCGAAGGTGGTAACATGGCCTTCCACAACCATTTGAATGATTTTGCTCATATTGAA GATCCTCTGGAAAGAAGAAGATTGGCTTTGGAGTCCATCGATGACGAAGGTTTCGGTTGG CAACAAGTTAAGACCATCTCCATTGCTGGTGTTGGTTTCTTGACAGATTCTTATGATATT TTTGCCATTAATTTGGGTATCACTATGATGTCCTACGTTTACTGGCACGGTAGTATGCCA GGTCCAAGTCAAACCTTGTTGAAGGTTTCCACTTCTGTTGGTACTGTTATTGGTCAATTT GGTTTTGGTACTTTAGCTGATATTGTTGGTCGTAAGAGAATTTATGGTATGGAACTTATT ATCATGATTGTCTGTACCATTCTGCAAACCACTGTTGCTCATTCTCCTGCTATTAACTTC GTTGCTGTTTTAACATTCTACCGTATTGTCATGGGTATTGGTATCGGTGGTGACTACCCA CTATCTTCTATTATTACTTCTGAATTTGCCACTACCAAATGGAGAGGTGCCATCATGGGT GCTGTCTTTGCTAACCAAGCTTGGGGTCAAATCTCCGGTGGTATCATCGCTCTTATCTTG GTTGCTGCTTACAAGGGCGAACTAGAATACGCAAACTCTGGTGCTGAATGTGATGCTAGA TGTCAAAAGGCTTGTGACCAAATGTGGAGAATCCTTATTGGGTTGGGTACCGTTCTAGGG TTGGCATGTTTGTATTTCAGATTAACTATTCCAGAATCTCCTAGATATCAATTGGATGTT AACGCTAAGTTGGAACTTGCTGCTGCCGCACAAGAACAAGATGGCGAAAAGAAAATTCAC GACACCAGTGATGAAGACATGGCAATTAACGGTTTGGAAAGAGCTTCTACTGCCGTCGAA TCTCTTGACAATCATCCTCCAAAGGCTTCGTTCAAAGATTTCTGCAGACATTTTGGTCAA TGGAAGTACGGTAAGATTTTGCTAGGTACTGCTGGTTCATGGTTTACCTTAGATGTTGCT TTCTACGGGTTGAGTTTAAACAGTGCTGTTATTCTGCAAACCATCGGTTATGCCGGTTCC AAAAACGTTTACAAGAAACTGTATGATACTGCTGTCGGTAATCTGATTTTGATTTGTGCT GGTTCATTACCTGGTTACTGGGTATCCGTCTTCACTGTCGATATAATCGGTAGAAAACCA ATTCAATTAGCCGGTTTCATCATCTTGACCGCTTTGTTCTGTGTCATCGGTTTCGCATAC CATAAACTTGGTGACCATGGTCTGTTGGCTCTTTACGTCATTTGTCAATTCTTCCAAAAC TTCGGTCCAAACACAACCACCTTTATTGTTCCTGGTGAGTGTTTCCCAACTCGTTACAGA TCTACTGCTCATGGTATTTCTGCTGCATCTGGTAAGGTCGGTGCCATTATTGCACAAACC GCTTTGGGTACTCTAATCGACCATAACTGTGCTAGAGACGGTAAGCCAACCAACTGTTGG TTACCTCACGTCATGGAAATTTTCGCCTTATTCATGTTGTTGGGTATCTTCACAACCTTG TTGATCCCAGAAACTAAGAGAAAGACTCTAGAAGAAATTAACGAGCTATACCACGATGAA ATCGATCCTGCTACGCTAAACTTCAGAAACAAGAATAATGACATTGAATCTTCCAGCCCA TCTCAACTTCAACATGAAGCATAA
Protein Properties
Pfam Domain Function
Protein Residues587
Protein Molecular Weight64381.5
Protein Theoretical pI6.41
Signalling Regions
  • None
Transmembrane Regions
  • 68-88
  • 109-129
  • 134-154
  • 157-177
  • 202-222
  • 251-271
  • 346-366
  • 396-416
  • 420-440
  • 443-463
  • 486-506
  • 523-543
Protein Sequence>Inorganic phosphate transporter PHO84 MSSVNKDTIHVAERSLHKEHLTEGGNMAFHNHLNDFAHIEDPLERRRLALESIDDEGFGW QQVKTISIAGVGFLTDSYDIFAINLGITMMSYVYWHGSMPGPSQTLLKVSTSVGTVIGQF GFGTLADIVGRKRIYGMELIIMIVCTILQTTVAHSPAINFVAVLTFYRIVMGIGIGGDYP LSSIITSEFATTKWRGAIMGAVFANQAWGQISGGIIALILVAAYKGELEYANSGAECDAR CQKACDQMWRILIGLGTVLGLACLYFRLTIPESPRYQLDVNAKLELAAAAQEQDGEKKIH DTSDEDMAINGLERASTAVESLDNHPPKASFKDFCRHFGQWKYGKILLGTAGSWFTLDVA FYGLSLNSAVILQTIGYAGSKNVYKKLYDTAVGNLILICAGSLPGYWVSVFTVDIIGRKP IQLAGFIILTALFCVIGFAYHKLGDHGLLALYVICQFFQNFGPNTTTFIVPGECFPTRYR STAHGISAASGKVGAIIAQTALGTLIDHNCARDGKPTNCWLPHVMEIFALFMLLGIFTTL LIPETKRKTLEEINELYHDEIDPATLNFRNKNNDIESSSPSQLQHEA
References
External Links
ResourceLink
Saccharomyces Genome Database PHO84
Uniprot IDP25297
Uniprot NamePHO84_YEAST
GenBank Gene IDZ49218
Genebank Protein ID805019
General Reference
  • Bun-Ya, M., Nishimura, M., Harashima, S., Oshima, Y. (1991). "The PHO84 gene of Saccharomyces cerevisiae encodes an inorganic phosphate transporter." Mol Cell Biol 11:3229-3238.2038328
  • Bowman, S., Churcher, C., Badcock, K., Brown, D., Chillingworth, T., Connor, R., Dedman, K., Devlin, K., Gentles, S., Hamlin, N., Hunt, S., Jagels, K., Lye, G., Moule, S., Odell, C., Pearson, D., Rajandream, M., Rice, P., Skelton, J., Walsh, S., Whitehead, S., Barrell, B. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Nature 387:90-93.9169872
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Peng, J., Schwartz, D., Elias, J. E., Thoreen, C. C., Cheng, D., Marsischky, G., Roelofs, J., Finley, D., Gygi, S. P. (2003). "A proteomics approach to understanding protein ubiquitination." Nat Biotechnol 21:921-926.12872131
  • Li, X., Gerber, S. A., Rudner, A. D., Beausoleil, S. A., Haas, W., Villen, J., Elias, J. E., Gygi, S. P. (2007). "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." J Proteome Res 6:1190-1197.17330950
  • Smolka, M. B., Albuquerque, C. P., Chen, S. H., Zhou, H. (2007). "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Proc Natl Acad Sci U S A 104:10364-10369.17563356
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956