N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase (P23797)

Identification
NameN-acetylglucosaminyl-phosphatidylinositol de-N-acetylase
SynonymsNot Available
Gene NameGPI12
Enzyme Class
Biological Properties
General FunctionInvolved in N-acetylglucosaminylphosphatidylinositol de
Specific FunctionInvolved in the second step of GPI biosynthesis. De-N- acetylation of N-acetylglucosaminyl-phosphatidylinositol
Cellular LocationEndoplasmic reticulum membrane
SMPDB PathwaysNot Available
KEGG Pathways
Glycosylphosphatidylinositol(GPI)-anchor biosynthesisec00563 Map00563
SMPDB ReactionsNot Available
KEGG Reactions
water + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol ↔ 6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + Acetic acid + hydron
Metabolites
YMDB IDNameView
YMDB00056Acetic acidShow
YMDB00862hydronShow
YMDB00890waterShow
GO ClassificationNot Available
Gene Properties
Chromosome Locationchromosome 13
LocusYMR281W
Gene Sequence>915 bp ATGAAGATGTTGAGGCGTACAAAGGTAAATTTTTCAAAATTGCTTTACAAGATTACTAAA CTAGCAATAGTACTGACGATCCTATATATTTATTTTACGCCCAAAATCGTCTCCCGAAAC AATGCATCATTGCAGCATATTTTTCCTCATAAATATGGCGATTATGAAATCAATTTGGTC ATAGCGCACCCTGACGACGAAGTTATGTTTTTTTCCCCCATAATTTCTCAACTGAATTCG TACTTTCCGAGAACCGTCCCATTTAACATAATCTGCTTATCAAAGGGCAACGCCGAAGGT CTTGGCGAAACCAGGGTAAGAGAATTAAATGAGTCGGCCGCTTTATTGCTACACAATGAA AGAGCAGTCTCCGTACAGGTGATGGATTTCCAGGATGGTATGGACGAAATATGGGATATT GATTCTATAACTTCTTCTCTTTCACAAAAGATAGATATAAAGAATCATAACTTGAACCAG ATTATCGTTACCTTTGATTCATATGGTGTATCAAATCATATCAACCACAAAAGCTGTTAT GCTGCCGTTAAAAAGTTGGTGGATGATTATGCTCAACCTAAGACCAAAAGAAATGAACAA CCACCTCATGTCACTGCGCTTTATTTGAGAAGCTACAAGAACAACATCGTTTTAAAGTAC AACTCCTTTATTTGGGAAATCCTAAAAATACTTTACGACCTGATTTCTCCATTCCGTAGA ATAATTCAGGCGCTTCCGCCTAACACAGCCGCCGAAAAAGACAAGCTTTCACTTATGAAT ACACATGCACAATACGTACTAGCGTTTGCCACTATGCTAAATGCTCACGAATCCCAAGTT GTGTGGTTTAGATACGGATGGTGGATATTTTCCAGATTTGTCTTCGTTAATGAATTTGAT GTTTATACATATTAG
Protein Properties
Pfam Domain Function
Protein Residues304
Protein Molecular Weight35445.60156
Protein Theoretical pI9.23
Signalling Regions
  • None
Transmembrane Regions
  • 21-38
Protein Sequence>N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase MKMLRRTKVNFSKLLYKITKLAIVLTILYIYFTPKIVSRNNASLQHIFPHKYGDYEINLV IAHPDDEVMFFSPIISQLNSYFPRTVPFNIICLSKGNAEGLGETRVRELNESAALLLHNE RAVSVQVMDFQDGMDEIWDIDSITSSLSQKIDIKNHNLNQIIVTFDSYGVSNHINHKSCY AAVKKLVDDYAQPKTKRNEQPPHVTALYLRSYKNNIVLKYNSFIWEILKILYDLISPFRR IIQALPPNTAAEKDKLSLMNTHAQYVLAFATMLNAHESQVVWFRYGWWIFSRFVFVNEFD VYTY
References
External Links
ResourceLink
Saccharomyces Genome Database GPI12
Uniprot IDP23797
Uniprot NameGPI12_YEAST
GenBank Gene IDAB017166
Genebank Protein ID4239988
General Reference
  • Watanabe, R., Ohishi, K., Maeda, Y., Nakamura, N., Kinoshita, T. (1999). "Mammalian PIG-L and its yeast homologue Gpi12p are N-acetylglucosaminylphosphatidylinositol de-N-acetylases essential in glycosylphosphatidylinositol biosynthesis." Biochem J 339 ( Pt 1):185-192.10085243
  • Finnegan, P. M., Payne, M. J., Keramidaris, E., Lukins, H. B. (1991). "Characterization of a yeast nuclear gene, AEP2, required for accumulation of mitochondrial mRNA encoding subunit 9 of the ATP synthase." Curr Genet 20:53-61.1718609
  • Bowman, S., Churcher, C., Badcock, K., Brown, D., Chillingworth, T., Connor, R., Dedman, K., Devlin, K., Gentles, S., Hamlin, N., Hunt, S., Jagels, K., Lye, G., Moule, S., Odell, C., Pearson, D., Rajandream, M., Rice, P., Skelton, J., Walsh, S., Whitehead, S., Barrell, B. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Nature 387:90-93.9169872