V-type proton ATPase subunit E (P22203)

Identification
NameV-type proton ATPase subunit E
Synonyms
  • V-ATPase subunit E
  • V-ATPase 27 kDa subunit
  • Vacuolar proton pump subunit E
Gene NameVMA4
Enzyme ClassNot Available
Biological Properties
General FunctionEnergy production and conversion
Specific FunctionSubunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells
Cellular LocationVacuole membrane; Peripheral membrane protein
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
GO Classification
Component
macromolecular complex
protein complex
proton-transporting two-sector ATPase complex, catalytic domain
Function
transporter activity
transmembrane transporter activity
substrate-specific transmembrane transporter activity
ion transmembrane transporter activity
proton-transporting ATPase activity, rotational mechanism
cation transmembrane transporter activity
inorganic cation transmembrane transporter activity
monovalent inorganic cation transmembrane transporter activity
hydrogen ion transmembrane transporter activity
Process
purine nucleotide biosynthetic process
metabolic process
purine nucleoside triphosphate biosynthetic process
purine ribonucleoside triphosphate biosynthetic process
ATP biosynthetic process
ATP synthesis coupled proton transport
nitrogen compound metabolic process
cellular nitrogen compound metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleobase, nucleoside and nucleotide metabolic process
nucleoside phosphate metabolic process
nucleotide metabolic process
purine nucleotide metabolic process
Gene Properties
Chromosome LocationNot Available
Locus
Gene Sequence>702 bp ATGTCCTCCGCTATTACTGCTTTGACACCAAATCAAGTGAACGATGAATTGAACAAGATG CAAGCTTTCATCAGAAAGGAAGCTGAAGAAAAAGCGAAGGAAATCCAATTGAAGGCTGAC CAAGAGTACGAAATCGAGAAGACCAACATCGTAAGAAATGAAACCAACAACATTGACGGC AACTTCAAGAGCAAGTTGAAGAAGGCCATGCTTTCGCAACAGATTACTAAGTCAACGATA GCAAACAAAATGAGGTTGAAGGTTCTTTCTGCTCGTGAACAGTCGCTAGAGCGGATATTC GAGGAAACCAAGGAAAAGTTGTCAGGAATTGCCAACAACCGGGACGAGTACAAGCCCATT TTGCAATCATTGATCGTGGAGGCTCTTTTGAAGTTGTTGGAACCTAAGGCGATTGTCAAG GCTCTTGAAAGAGACGTCGATTTAATCGAGTCGATGAAGGACGACATTATGCGTGAGTAT GGGGAAAAGGCCCAGCGCGCACCATTGGAAGAGATTGTTATCTCCAATGATTACTTGAAC AAAGACCTTGTTTCCGGCGGTGTTGTGGTCTCCAATGCATCAGACAAGATTGAAATTAAC AACACTTTGGAGGAAAGATTGAAATTGTTAAGTGAAGAGGCATTGCCCGCCATCAGATTG GAATTGTATGGTCCTTCCAAGACAAGAAAGTTCTTTGATTGA
Protein Properties
Pfam Domain Function
Protein Residues233
Protein Molecular Weight26471.09961
Protein Theoretical pI5.01
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>V-type proton ATPase subunit E MSSAITALTPNQVNDELNKMQAFIRKEAEEKAKEIQLKADQEYEIEKTNIVRNETNNIDG NFKSKLKKAMLSQQITKSTIANKMRLKVLSAREQSLDGIFEETKEKLSGIANNRDEYKPI LQSLIVEALLKLLEPKAIVKALERDVDLIESMKDDIMREYGEKAQRAPLEEIVISNDYLN KDLVSGGVVVSNASDKIEINNTLEERLKLLSEEALPAIRLELYGPSKTRKFFD
References
External Links
ResourceLink
Saccharomyces Genome Database VMA4
Uniprot IDP22203
Uniprot NameVATE_YEAST
GenBank Gene IDM60663
Genebank Protein ID173169
General Reference
  • Foury, F. (1990). "The 31-kDa polypeptide is an essential subunit of the vacuolar ATPase in Saccharomyces cerevisiae." J Biol Chem 265:18554-18560.2145285
  • Parle-McDermott, A. G., Hand, N. J., Goulding, S. E., Wolfe, K. H. (1996). "Sequence of 29 kb around the PDR10 locus on the right arm of Saccharomyces cerevisiae chromosome XV: similarity to part of chromosome I." Yeast 12:999-1004.8896263
  • Dujon, B., Albermann, K., Aldea, M., Alexandraki, D., Ansorge, W., Arino, J., Benes, V., Bohn, C., Bolotin-Fukuhara, M., Bordonne, R., Boyer, J., Camasses, A., Casamayor, A., Casas, C., Cheret, G., Cziepluch, C., Daignan-Fornier, B., Dang, D. V., de Haan, M., Delius, H., Durand, P., Fairhead, C., Feldmann, H., Gaillon, L., Kleine, K., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Nature 387:98-102.9169874
  • Ho, M. N., Hill, K. J., Lindorfer, M. A., Stevens, T. H. (1993). "Isolation of vacuolar membrane H(+)-ATPase-deficient yeast mutants; the VMA5 and VMA4 genes are essential for assembly and activity of the vacuolar H(+)-ATPase." J Biol Chem 268:221-227.8416931
  • Tomashek, J. J., Sonnenburg, J. L., Artimovich, J. M., Klionsky, D. J. (1996). "Resolution of subunit interactions and cytoplasmic subcomplexes of the yeast vacuolar proton-translocating ATPase." J Biol Chem 271:10397-10404.8626613
  • Zhang, J. W., Parra, K. J., Liu, J., Kane, P. M. (1998). "Characterization of a temperature-sensitive yeast vacuolar ATPase mutant with defects in actin distribution and bud morphology." J Biol Chem 273:18470-18480.9660816
  • Seol, J. H., Shevchenko, A., Shevchenko, A., Deshaies, R. J. (2001). "Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly." Nat Cell Biol 3:384-391.11283612
  • Huh, W. K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., O'Shea, E. K. (2003). "Global analysis of protein localization in budding yeast." Nature 425:686-691.14562095
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Jones, R. P., Durose, L. J., Findlay, J. B., Harrison, M. A. (2005). "Defined sites of interaction between subunits E (Vma4p), C (Vma5p), and G (Vma10p) within the stator structure of the vacuolar H+-ATPase." Biochemistry 44:3933-3941.15751969
  • Owegi, M. A., Carenbauer, A. L., Wick, N. M., Brown, J. F., Terhune, K. L., Bilbo, S. A., Weaver, R. S., Shircliff, R., Newcomb, N., Parra-Belky, K. J. (2005). "Mutational analysis of the stator subunit E of the yeast V-ATPase." J Biol Chem 280:18393-18402.15718227
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956