V-type proton ATPase subunit B (P16140)

Identification
NameV-type proton ATPase subunit B
Synonyms
  • V-ATPase subunit B
  • V-ATPase 57 kDa subunit
  • Vacuolar proton pump subunit B
Gene NameVMA2
Enzyme ClassNot Available
Biological Properties
General FunctionEnergy production and conversion
Specific FunctionNon-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. It is an electrogenic proton pump that generates a proton motive force of 180 mv, inside positive and acidic, in the vacuolar membrane vesicles
Cellular LocationCytoplasmic
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
GO Classification
Component
macromolecular complex
protein complex
proton-transporting two-sector ATPase complex, catalytic domain
proton-transporting two-sector ATPase complex
proton-transporting V-type ATPase, V1 domain
Function
proton-transporting ATPase activity, rotational mechanism
hydrogen ion transporting ATP synthase activity, rotational mechanism
binding
catalytic activity
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances
transporter activity
transmembrane transporter activity
substrate-specific transmembrane transporter activity
ion transmembrane transporter activity
hydrolase activity
cation transmembrane transporter activity
hydrolase activity, acting on acid anhydrides
inorganic cation transmembrane transporter activity
nucleoside binding
monovalent inorganic cation transmembrane transporter activity
purine nucleoside binding
hydrogen ion transmembrane transporter activity
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
Process
nucleobase, nucleoside and nucleotide metabolic process
metabolic process
nucleoside phosphate metabolic process
nucleotide metabolic process
establishment of localization
purine nucleotide metabolic process
transport
purine nucleotide biosynthetic process
purine nucleoside triphosphate biosynthetic process
purine ribonucleoside triphosphate biosynthetic process
hydrogen transport
ATP biosynthetic process
proton transport
ATP synthesis coupled proton transport
purine nucleoside triphosphate metabolic process
purine ribonucleoside triphosphate metabolic process
ATP metabolic process
nitrogen compound metabolic process
cellular nitrogen compound metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
Gene Properties
Chromosome LocationNot Available
Locus
Gene Sequence>1554 bp ATGGTTTTGTCTGATAAGGAGTTGTTTGCCATAAATAAGAAAGCCGTCGAACAAGGTTTC AATGTGAAGCCTAGATTGAACTATAATACGGTCAGTGGTGTGAACGGTCCATTAGTCATT TTGGAAAAGGTCAAGTTCCCACGTTACAACGAAATTGTTAATTTGACATTGCCAGATGGA ACCGTGAGACAAGGTCAAGTTTTGGAAATTAGAGGAGATAGAGCCATTGTGCAAGTGTTT GAAGGTACATCTGGTATTGATGTCAAGAAGACTACCGTGGAATTCACTGGTGAGAGTTTG AGAATTCCTGTGTCTGAAGACATGTTGGGTAGAATTTTTGACGGTTCTGGTAGACCCATT GACAACGGTCCTAAAGTTTTCGCAGAGGATTACTTGGACATTAACGGTTCTCCTATCAAC CCATATGCTCGTATTTATCCAGAAGAAATGATTTCTACTGGTGTTTCTGCTATTGACACA ATGAACTCCATTGCCAGAGGTCAAAAGATCCCAATTTTCTCCGCATCAGGTTTACCACAC AACGAAATTGCAGCACAAATTTGTAGACAGGCTGGTTTGGTGAGACCTACCAAGGATGTT CATGATGGTCATGAAGAAAATTTCTCCATCGTTTTTGCTGCCATGGGTGTCAACTTGGAA ACCGCTAGATTTTTCAAACAGGATTTCGAAGAAAATGGGTCTTTGGAAAGAACTTCATTA TTTTTGAACTTGGCTAATGACCCTACCATTGAAAGAATTATCACTCCAAGATTGGCCTTG ACCACCGCTGAATACCTTGCTTACCAAACGGAACGTCATGTGTTGACCATCTTGACCGAT ATGTCATCGTATGCTGATGCTCTTAGAGAAGTTTCCGCTGCTAGAGAAGAAGTTCCAGGT AGAAGAGGTTATCCTGGTTACATGTATACAGATTTGTCCACAATTTATGAAAGAGCAGGT AGAGTAGAGGGTCGTAACGGGTCCATCACTCAAATACCTATCTTGACAATGCCTAACGAT GATATTACGCATCCAATTCCGGATTTGACCGGTTATATTACCGAGGGTCAAATCTTCGTT GACCGTCAATTACATAACAAGGGTATCTACCCACCAATCAACGTCTTGCCTTCGTTGAGT AGATTGATGAAATCTGCCATCGGTGAAGGTATGACCAGAAAGGACCACGGTGACGTTTCT AACCAATTGTATGCCAAGTACGCCATCGGTAAGGACGCTGCTGCTATGAAGGCCGTTGTC GGTGAAGAGGCGTTATCCATCGAAGATAAGTTATCTTTGGAATTTTTGGAAAAATTCGAA AAGACCTTTATCACACAAGGCGCCTACGAGGACAGAACCGTTTTCGAAAGTTTGGACCAG GCATGGAGTTTGCTAAGAATCTACCCTAAGGAGATGTTGAATAGAATCTCCCCAAAGATT CTTGATGAATTTTACGATAGAGCCAGAGACGATGCCGACGAAGATGAAGAAGATCCCGAC ACAAGAAGCTCCGGTAAGAAGAAGGACGCCAGCCAAGAAGAATCTCTAATCTAA
Protein Properties
Pfam Domain Function
Protein Residues517
Protein Molecular Weight57748.69922
Protein Theoretical pI4.69
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>V-type proton ATPase subunit B MVLSDKELFAINKKAVEQGFNVKPRLNYNTVSGVNGPLVILEKVKFPRYNEIVNLTLPDG TVRQGQVLEIRGDRAIVQVFEGTSGIDVKKTTVEFTGESLRIPVSEDMLGRIFDGSGRPI DNGPKVFAEDYLDINGSPINPYARIYPEEMISTGVSAIDTMNSIARGQKIPIFSASGLPH NEIAAQICRQAGLVRPTKDVHDGHEENFSIVFAAMGVNLETARFFKQDFEENGSLERTSL FLNLANDPTIERIITPRLALTTAEYLAYQTERHVLTILTDMSSYADALREVSAAREEVPG RRGYPGYMYTDLSTIYERAGRVEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIFV DRQLHNKGIYPPINVLPSLSRLMKSAIGEGMTRKDHGDVSNQLYAKYAIGKDAAAMKAVV GEEALSIEDKLSLEFLEKFEKTFITQGAYEDRTVFESLDQAWSLLRIYPKEMLNRISPKI LDEFYDRARDDADEDEEDPDTRSSGKKKDASQEESLI
References
External Links
ResourceLink
Saccharomyces Genome Database VMA2
Uniprot IDP16140
Uniprot NameVATB_YEAST
GenBank Gene IDAY693158
Genebank Protein ID51013767
General Reference
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  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Gruhler, A., Olsen, J. V., Mohammed, S., Mortensen, P., Faergeman, N. J., Mann, M., Jensen, O. N. (2005). "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Mol Cell Proteomics 4:310-327.15665377
  • Li, X., Gerber, S. A., Rudner, A. D., Beausoleil, S. A., Haas, W., Villen, J., Elias, J. E., Gygi, S. P. (2007). "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." J Proteome Res 6:1190-1197.17330950
  • Chi, A., Huttenhower, C., Geer, L. Y., Coon, J. J., Syka, J. E., Bai, D. L., Shabanowitz, J., Burke, D. J., Troyanskaya, O. G., Hunt, D. F. (2007). "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Proc Natl Acad Sci U S A 104:2193-2198.17287358
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