| Identification |
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| Name | Proline-specific permease |
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| Synonyms | Not Available |
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| Gene Name | PUT4 |
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| Enzyme Class | Not Available |
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| Biological Properties |
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| General Function | Involved in transport |
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| Specific Function | Required for high-affinity proline transport. May be responsible for proline recognition and probably also for proline translocation across the plasma membrane. Also function as non- specific GABA permease. Can also transport alanine and glycine |
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| Cellular Location | Membrane; Multi-pass membrane protein |
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| SMPDB Pathways | Not Available |
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| KEGG Pathways | Not Available |
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| SMPDB Reactions | Not Available |
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| KEGG Reactions | Not Available |
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| Metabolites | | YMDB ID | Name | View |
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| YMDB00016 | Glycine | Show | | YMDB00154 | L-Alanine | Show | | YMDB00335 | gamma-Aminobutyric acid | Show | | YMDB00378 | L-Proline | Show | | YMDB00862 | hydron | Show |
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| GO Classification | | Component |
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| cell part | | membrane | | membrane part | | intrinsic to membrane | | integral to membrane | | Function |
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| amine transmembrane transporter activity | | amino acid transmembrane transporter activity | | transporter activity | | transmembrane transporter activity | | active transmembrane transporter activity | | Process |
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| amine transport | | amino acid transport | | transmembrane transport | | establishment of localization | | transport |
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| Gene Properties |
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| Chromosome Location | chromosome 15 |
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| Locus | YOR348C |
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| Gene Sequence | >1884 bp
ATGGTAAATATACTGCCCTTCCACAAGAACAATAGACACAGCGCGGGAGTCGTCACCTGC
GCGGACGATGTTAGCGGCGACGGTAGCGGCGGCGACACCAAGAAGGAGGAGAATGTTGTC
CAGGTAACGGAATCACCATCGTCCGGGTCGCGCAATAATCATCGCAGCGACAATGAAAAA
GATGACGCCATCCGTATGGAGAAAATATCTAAGAACCAGTCCGCGTCGTCCAACGGCACC
ATCCGCGAGGATTTGATTATGGACGTGGACTTGGAGAAATCGCCCTCCGTCGATGGCGAT
AGCGAGCCGCACAAACTAAAACAAGGTTTGCAGTCGCGCCATGTGCAACTGATCGCGCTG
GGCGGCGCCATCGGTACCGGTTTGCTGGTGGGGACTTCATCCACGCTTCATACGTGCGGT
CCCGCGGGGCTGTTCATATCGTACATTATTATTTCCGCGGTGATCTACCCGATCATGTGC
GCGCTGGGCGAAATGGTGTGCTTTTTGCCCGGTGATGGTTCTGACAGTGCCGGGTCGACG
GCCAATCTGGTCACTAGGTACGTTGACCCTTCGTTGGGCTTCGCCACTGGGTGGAATTAC
TTCTACTGCTACGTCATTTTAGTGGCCGCCGAGTGTACGGCGGCATCCGGTGTGGTCGAA
TACTGGACCACCGCGGTTCCCAAGGGCGTTTGGATCACGATCTTTCTATGCGTGGTTGTT
ATACTAAACTTTTCCGCGGTCAAAGTGTACGGCGAATCCGAGTTTTGGTTCGCGTCTATC
AAGATATTATGCATTGTGGGACTGATCATTCTGTCCTTTATTTTGTTTTGGGGTGGTGGC
CCCAACCATGACCGTCTTGGCTTCAGATACTGGCAACATCCGGGCGCTTTTGCGCATCAC
CTCACGGGCGGATCGCTGGGTAACTTCACAGATATCTACACTGGGATTATCAAGGGCGCC
TTCGCCTTCATTCTTGGTCCGGAACTGGTGTGCATGACTTCCGCGGAATGCGCGGACCAG
CGTAGAAATATCGCTAAGGCTTCGCGCCGCTTTGTATGGAGACTGATCTTCTTCTACGTT
CTGGGGACGCTGGCCATCTCCGTTATTGTTCCATATAATGACCCAACATTGGTAAATGCG
CTGGCGCAGGGCAAACCGGGAGCCGGCTCGTCACCCTTTGTGATCGGAATTCAAAACGCC
GGGATTAAGGTTCTTCCCCACATTATCAATGGGTGTATCTTGACCAGCGCGTGGTCTGCC
GCCAATGCGTTTATGTTTGCGAGCACAAGATCCCTGTTGACCATGGCGCAAACGGGACAG
GCACCCAAATGTTTGGGCAGAATCAACAAATGGGGTGTTCCATACGTGGCTGTGGGTGTT
TCCTTCTTGTGTTCTTGTTTGGCATATCTGAACGTGTCTTCATCCACGGCAGACGTGTTC
AATTGGTTTTCCAATATCAGCACCATTTCCGGGTTTCTGGGCTGGATGTGCGGCTGCATC
GCGTACCTGAGATTCCGCAAGGCTATTTTCTACAATGGCCTCTACGACAGATTGCCTTTC
AAGACGTGGGGACAGCCTTACACCGTGTGGTTCTCTCTCATTGTTATAGGCATCATCACC
ATTACCAACGGGTATGCCATTTTCATCCCTAAGTACTGGAGAGTAGCAGATTTCATAGCT
GCCTACATCACCCTACCCATCTTCCTGGTTTTGTGGTTCGGCCATAAGCTGTATACTCGG
ACGTGGAGACAATGGTGGCTCCCTGTGTCCGAGATCGATGTTACTACAGGGCTAGTCGAG
ATCGAAGAGAAATCAAGAGAAATTGAGGAGATGAGATTACCCCCCACCGGTTTCAAAGAC
AAGTTCTTGGACGCCTTGTTGTAA |
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| Protein Properties |
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| Pfam Domain Function | |
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| Protein Residues | 627 |
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| Protein Molecular Weight | 68786.79688 |
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| Protein Theoretical pI | 7.59 |
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| Signalling Regions | |
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| Transmembrane Regions | - 115-136
- 142-162
- 191-210
- 229-247
- 262-281
- 306-326
- 351-370
- 408-427
- 455-473
- 483-503
- 522-542
- 558-578
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| Protein Sequence | >Proline-specific permease
MVNILPFHKNNRHSAGVVTCADDVSGDGSGGDTKKEEDVVQVTESPSSGSRNNHRSDNEK
DDAIRMEKISKNQSASSNGTIREDLIMDVDLEKSPSVDGDSEPHKLKQGLQSRHVQLIAL
GGAIGTGLLVGTSSTLHTCGPAGLFISYIIISAVIYPIMCALGEMVCFLPGDGSDSAGST
ANLVTRYVDPSLGFATGWNYFYCYVILVAAECTAASGVVEYWTTAVPKGVWITIFLCVVV
ILNFSAVKVYGESEFWFASIKILCIVGLIILSFILFWGGGPNHDRLGFRYWQHPGAFAHH
LTGGSLGNFTDIYTGIIKGAFAFILGPELVCMTSAECADQRRNIAKASRRFVWRLIFFYV
LGTLAISVIVPYNDPTLVNALAQGKPGAGSSPFVIGIQNAGIKVLPHIINGCILTSAWSA
ANAFMFASTRSLLTMAQTGQAPKCLGRINKWGVPYVAVGVSFLCSCLAYLNVSSSTADVF
NWFSNISTISGFLGWMCGCIAYLRFRKAIFYNGLYDRLPFKTWGQPYTVWFSLIVIGIIT
ITNGYAIFIPKYWRVADFIAAYITLPIFLVLWFGHKLYTRTWRQWWLPVSEIDVTTGLVE
IEEKSREIEEMRLPPTGFKDKFLDALL |
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| References |
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| External Links | |
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| General Reference | - Vandenbol, M., Jauniaux, J. C., Grenson, M. (1989). "Nucleotide sequence of the Saccharomyces cerevisiae PUT4 proline-permease-encoding gene: similarities between CAN1, HIP1 and PUT4 permeases." Gene 83:153-159.2687114
- Purnelle, B., Goffeau, A. (1996). "Nucleotide sequence analysis of a 40 kb segment on the right arm of yeast chromosome XV reveals 18 open reading frames including a new pyruvate kinase and three homologues to chromosome I genes." Yeast 12:1475-1481.8948102
- Dujon, B., Albermann, K., Aldea, M., Alexandraki, D., Ansorge, W., Arino, J., Benes, V., Bohn, C., Bolotin-Fukuhara, M., Bordonne, R., Boyer, J., Camasses, A., Casamayor, A., Casas, C., Cheret, G., Cziepluch, C., Daignan-Fornier, B., Dang, D. V., de Haan, M., Delius, H., Durand, P., Fairhead, C., Feldmann, H., Gaillon, L., Kleine, K., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Nature 387:98-102.9169874
- Regenberg, B., During-Olsen, L., Kielland-Brandt, M. C., Holmberg, S. (1999). "Substrate specificity and gene expression of the amino-acid permeases in Saccharomyces cerevisiae." Curr Genet 36:317-328.10654085
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