| Identification |
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| Name | Mitogen-activated protein kinase KSS1 |
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| Synonyms | - MAP kinase KSS1
- Kinase suppressor of SST2
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| Gene Name | KSS1 |
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| Enzyme Class | Not Available |
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| Biological Properties |
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| General Function | signal transduction involved in filamentous growth |
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| Specific Function | Together with closely related FUS3, KSS1 is the final kinase in the signal transduction cascade regulating activation/repression of the mating and filamentation pathways, induced by pheromone and nitrogen/carbon limitation, respectively. Phosphorylated KSS1 activates both pathways, whereas activated FUS3 activates the mating but suppresses the filamentation pathway. KSS1 activity is down-regulated by FUS3 during pheromone induction to prevent inappropriate activation of the filamentation pathway. During induction of filamentation, KSS1 activates the transcription factor STE12 resulting in its binding to and activation of filamentation specific genes. Non-activated KSS1 has a kinase-independent repressive effect on STE12 transcriptional activity, that is mediated by direct binding to STE12 and depends on the presence of DIG1 and DIG2, and that is required for the suppression of filamentation under normal growth conditions. SSN3/SRB10 contributes further to the suppression of filamentation under these conditions by reducing STE12 stability independent of KSS1. FUS3 can partially compensate for the lack of KSS1 but filamentation becomes constitutively induced at a low level in the absence of any signal. KSS1 phosphorylates STE7, STE5, FAR1, DIG1, DIG2, STE12, and SST2. |
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| Cellular Location | Not Available |
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| SMPDB Pathways | | Stress-activated signalling pathways: starvation test 1 | PW003483 |    |
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| KEGG Pathways | Not Available |
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| SMPDB Reactions | Not Available |
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| KEGG Reactions | Not Available |
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| Metabolites | |
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| GO Classification | | Function |
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| ATP binding | | pheromone-dependent signal transduction involved in conjugation with cellular fusion | | nucleus | | cytoplasm | | invasive growth in response to glucose limitation | | protein phosphorylation | | signal transduction involved in filamentous growth | | periplasmic space | | MAP kinase activity | | transcription factor binding | | cell cycle | | negative regulation of sequence-specific DNA binding transcription factor activity |
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| Gene Properties |
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| Chromosome Location | Not Available |
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| Locus | |
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| Gene Sequence | >1107 bp
ATGGCTAGAACCATAACTTTTGATATCCCTTCCCAATATAAACTCGTAGATTTAATAGGT
GAGGGAGCGTACGGAACAGTATGTTCAGCAATTCATAAGCCTTCCGGCATAAAGGTAGCT
ATCAAGAAAATACAACCGTTTAGCAAAAAATTGTTTGTTACAAGAACTATACGTGAGATC
AAGCTTTTACGGTATTTCCATGAACACGAAAACATAATAAGTATATTGGATAAAGTAAGG
CCAGTATCCATAGACAAACTAAACGCTGTTTATTTAGTCGAAGAGTTGATGGAAACCGAT
TTACAAAAAGTAATTAATAATCAGAATAGCGGGTTTTCCACTTTAAGTGATGACCATGTT
CAATACTTTACATACCAAATCCTCAGAGCCTTAAAGTCTATTCACAGTGCACAAGTTATC
CATAGAGACATAAAGCCATCAAACCTGTTACTAAATTCCAATTGTGATCTCAAAGTCTGC
GATTTTGGACTAGCGAGGTGTTTAGCTAGCAGTAGCGATTCAAGAGAAACATTGGTAGGA
TTCATGACGGAGTACGTCGCAACGCGATGGTACAGGGCACCCGAGATAATGCTAACTTTT
CAAGAGTACACAACTGCGATGGATATATGGTCATGCGGATGCATTTTGGCTGAAATGGTC
TCCGGGAAGCCTTTGTTCCCAGGCAGAGACTATCATCATCAATTATGGCTAATTCTAGAA
GTCTTGGGAACTCCATCTTTCGAAGACTTTAATCAGATCAAATCCAAGAGGGCTAAAGAG
TATATAGCAAACTTACCTATGAGGCCACCCTTGCCATGGGAGACCGTCTGGTCAAAGACC
GATCTGAATCCAGATATGATAGATTTACTAGACAAAATGCTTCAATTCAATCCTGACAAA
AGAATAAGCGCAGCAGAAGCTTTAAGACACCCTTACCTGGCAATGTACCATGACCCAAGT
GATGAGCCGGAATATCCTCCACTTAATTTGGATGATGAATTTTGGAAACTGGATAACAAG
ATAATGCGTCCGGAAGAGGAGGAAGAAGTGCCCATAGAAATGCTCAAAGACATGCTTTAC
GATGAACTAATGAAGACCATGGAATAG |
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| Protein Properties |
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| Pfam Domain Function | Not Available |
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| Protein Residues | 368 |
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| Protein Molecular Weight | 42691.845 |
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| Protein Theoretical pI | Not Available |
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| Signalling Regions | Not Available |
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| Transmembrane Regions | Not Available |
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| Protein Sequence | >Mitogen-activated protein kinase KSS1
MARTITFDIPSQYKLVDLIGEGAYGTVCSAIHKPSGIKVAIKKIQPFSKKLFVTRTIREI
KLLRYFHEHENIISILDKVRPVSIDKLNAVYLVEELMETDLQKVINNQNSGFSTLSDDHV
QYFTYQILRALKSIHSAQVIHRDIKPSNLLLNSNCDLKVCDFGLARCLASSSDSRETLVG
FMTEYVATRWYRAPEIMLTFQEYTTAMDIWSCGCILAEMVSGKPLFPGRDYHHQLWLILE
VLGTPSFEDFNQIKSKRAKEYIANLPMRPPLPWETVWSKTDLNPDMIDLLDKMLQFNPDK
RISAAEALRHPYLAMYHDPSDEPEYPPLNLDDEFWKLDNKIMRPEEEEEVPIEMLKDMLY
DELMKTME |
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| References |
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| External Links | | Resource | Link |
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| Saccharomyces Genome Database | KSS1 | | Uniprot ID | P14681 | | Uniprot Name | KSS1 |
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| General Reference | Not Available |
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