Mitogen-activated protein kinase KSS1 (P14681)

Identification
NameMitogen-activated protein kinase KSS1
Synonyms
  • MAP kinase KSS1
  • Kinase suppressor of SST2
Gene NameKSS1
Enzyme ClassNot Available
Biological Properties
General Functionsignal transduction involved in filamentous growth
Specific FunctionTogether with closely related FUS3, KSS1 is the final kinase in the signal transduction cascade regulating activation/repression of the mating and filamentation pathways, induced by pheromone and nitrogen/carbon limitation, respectively. Phosphorylated KSS1 activates both pathways, whereas activated FUS3 activates the mating but suppresses the filamentation pathway. KSS1 activity is down-regulated by FUS3 during pheromone induction to prevent inappropriate activation of the filamentation pathway. During induction of filamentation, KSS1 activates the transcription factor STE12 resulting in its binding to and activation of filamentation specific genes. Non-activated KSS1 has a kinase-independent repressive effect on STE12 transcriptional activity, that is mediated by direct binding to STE12 and depends on the presence of DIG1 and DIG2, and that is required for the suppression of filamentation under normal growth conditions. SSN3/SRB10 contributes further to the suppression of filamentation under these conditions by reducing STE12 stability independent of KSS1. FUS3 can partially compensate for the lack of KSS1 but filamentation becomes constitutively induced at a low level in the absence of any signal. KSS1 phosphorylates STE7, STE5, FAR1, DIG1, DIG2, STE12, and SST2.
Cellular LocationNot Available
SMPDB Pathways
Stress-activated signalling pathways: starvation test 1PW003483 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
GO Classification
Function
nucleus
cytoplasm
invasive growth in response to glucose limitation
protein phosphorylation
signal transduction involved in filamentous growth
periplasmic space
MAP kinase activity
transcription factor binding
cell cycle
negative regulation of sequence-specific DNA binding transcription factor activity
ATP binding
pheromone-dependent signal transduction involved in conjugation with cellular fusion
Gene Properties
Chromosome LocationNot Available
Locus
Gene Sequence>1107 bp ATGGCTAGAACCATAACTTTTGATATCCCTTCCCAATATAAACTCGTAGATTTAATAGGT GAGGGAGCGTACGGAACAGTATGTTCAGCAATTCATAAGCCTTCCGGCATAAAGGTAGCT ATCAAGAAAATACAACCGTTTAGCAAAAAATTGTTTGTTACAAGAACTATACGTGAGATC AAGCTTTTACGGTATTTCCATGAACACGAAAACATAATAAGTATATTGGATAAAGTAAGG CCAGTATCCATAGACAAACTAAACGCTGTTTATTTAGTCGAAGAGTTGATGGAAACCGAT TTACAAAAAGTAATTAATAATCAGAATAGCGGGTTTTCCACTTTAAGTGATGACCATGTT CAATACTTTACATACCAAATCCTCAGAGCCTTAAAGTCTATTCACAGTGCACAAGTTATC CATAGAGACATAAAGCCATCAAACCTGTTACTAAATTCCAATTGTGATCTCAAAGTCTGC GATTTTGGACTAGCGAGGTGTTTAGCTAGCAGTAGCGATTCAAGAGAAACATTGGTAGGA TTCATGACGGAGTACGTCGCAACGCGATGGTACAGGGCACCCGAGATAATGCTAACTTTT CAAGAGTACACAACTGCGATGGATATATGGTCATGCGGATGCATTTTGGCTGAAATGGTC TCCGGGAAGCCTTTGTTCCCAGGCAGAGACTATCATCATCAATTATGGCTAATTCTAGAA GTCTTGGGAACTCCATCTTTCGAAGACTTTAATCAGATCAAATCCAAGAGGGCTAAAGAG TATATAGCAAACTTACCTATGAGGCCACCCTTGCCATGGGAGACCGTCTGGTCAAAGACC GATCTGAATCCAGATATGATAGATTTACTAGACAAAATGCTTCAATTCAATCCTGACAAA AGAATAAGCGCAGCAGAAGCTTTAAGACACCCTTACCTGGCAATGTACCATGACCCAAGT GATGAGCCGGAATATCCTCCACTTAATTTGGATGATGAATTTTGGAAACTGGATAACAAG ATAATGCGTCCGGAAGAGGAGGAAGAAGTGCCCATAGAAATGCTCAAAGACATGCTTTAC GATGAACTAATGAAGACCATGGAATAG
Protein Properties
Pfam Domain FunctionNot Available
Protein Residues368
Protein Molecular Weight42691.845
Protein Theoretical pINot Available
Signalling RegionsNot Available
Transmembrane RegionsNot Available
Protein Sequence>Mitogen-activated protein kinase KSS1 MARTITFDIPSQYKLVDLIGEGAYGTVCSAIHKPSGIKVAIKKIQPFSKKLFVTRTIREI KLLRYFHEHENIISILDKVRPVSIDKLNAVYLVEELMETDLQKVINNQNSGFSTLSDDHV QYFTYQILRALKSIHSAQVIHRDIKPSNLLLNSNCDLKVCDFGLARCLASSSDSRETLVG FMTEYVATRWYRAPEIMLTFQEYTTAMDIWSCGCILAEMVSGKPLFPGRDYHHQLWLILE VLGTPSFEDFNQIKSKRAKEYIANLPMRPPLPWETVWSKTDLNPDMIDLLDKMLQFNPDK RISAAEALRHPYLAMYHDPSDEPEYPPLNLDDEFWKLDNKIMRPEEEEEVPIEMLKDMLY DELMKTME
References
External Links
ResourceLink
Saccharomyces Genome Database KSS1
Uniprot IDP14681
Uniprot NameKSS1
General ReferenceNot Available