Leucyl-tRNA synthetase, mitochondrial (P11325)

Identification
NameLeucyl-tRNA synthetase, mitochondrial
Synonyms
  • Leucine--tRNA ligase
  • LeuRS
Gene NameNAM2
Enzyme Class
Biological Properties
General FunctionInvolved in nucleotide binding
Specific FunctionATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)
Cellular LocationMitochondrion matrix
SMPDB PathwaysNot Available
KEGG Pathways
Valine, leucine and isoleucine biosynthesisec00290 Map00290
SMPDB ReactionsNot Available
KEGG Reactions
L-Leucine + Adenosine triphosphate + tRNA(Leu) → Adenosine monophosphate + Pyrophosphate + Leu-tRNA(Leu)
Metabolites
YMDB IDNameView
YMDB00097Adenosine monophosphateShow
YMDB00109Adenosine triphosphateShow
YMDB00219PyrophosphateShow
YMDB00387L-LeucineShow
GO Classification
Component
intracellular part
cytoplasm
cell part
Function
ligase activity
binding
nucleoside binding
purine nucleoside binding
adenyl nucleotide binding
leucine-tRNA ligase activity
adenyl ribonucleotide binding
ATP binding
ligase activity, forming carbon-oxygen bonds
ligase activity, forming aminoacyl-tRNA and related compounds
catalytic activity
aminoacyl-tRNA ligase activity
nucleotide binding
Process
ncRNA metabolic process
biosynthetic process
tRNA metabolic process
tRNA aminoacylation
tRNA aminoacylation for protein translation
macromolecule biosynthetic process
cellular macromolecule biosynthetic process
translation
leucyl-tRNA aminoacylation
metabolic process
macromolecule metabolic process
cellular macromolecule metabolic process
RNA metabolic process
Gene Properties
Chromosome Locationchromosome 12
LocusYLR382C
Gene Sequence>2685 bp ATGCTGTCTCGACCTTCAAGCCGATTCCTATCCACTAAAAGGGGCCCTGGACCTGCAGTG AAAAAATTAATTGCAATTGGGGAGAAATGGAAACAGAAGACAACCCGTGGCTTGCCTAAG CAGGATACTCTGAATAGCGGGTCGAAATATATTCTGTGCCAGTTTCCATATCCTTCTGGG GCGCTTCACATAGGACATCTCCGAGTTTATGTCATTAGCGACTCTTTGAATAGATTTTAC AAACAAAAAGGGTACAACGTGATACATCCGATGGGATGGGATGCTTTTGGGTTACCTGCC GAGAACGCTGCTATAGAAAGAAGCATTAATCCGGCCATATGGACTAGGGACAATATTGCA AAAATGAAACAACAAATGCAAAGTATGCTGGCAAATTTTGATTGGGACAGAGAAATAACT ACATGTGATCCCGAATACTATAAATTCACCCAATGGATTTTCTTAAAACTTTTTGAAAAT GGCTTAGCTTATCGTAAAGAAGCAGAAATTAATTGGGATCCCGTTGATATGACAGTTTTG GCTAATGAACAAGTGGATGCCCAGGGCCGTTCTTGGAGATCAGGGGCTATTGTGGAAAAG AAGCAGCTAAAACAGTGGTTTTTGGGAATAACGAAATTCGCTCCTAAATTGAAAAAGCAC TTGAACCAACTGAAGGACTGGCCTTCCAACGTGAAGCAAATGCAAAAAAATTGGATAGGC GAATCTGTGGGTGCAGAATTAGTGTTCAAAGTTGCGGACCCCAAATTTGAAAACTTGATT GTCTTTACAACAAGACCGGAAACTCTTTTTGCTGTACAGTATGTTGCTCTCGCATTAGAC CACCCAATTGTACAAAAATACTGCGAGGAAATGCCGGATTTAAAAGAGTTTATACAGAAA AGTGATCAATTACCAAACGATACGAAGGAAGGATTTCAGTTACCTAACATAAAGGCCGTA AATCCCTTAACTAAAGAGGAAGTCCCCATATTTGCAGCTCCATATGTGGTCAGCAGCTAT GGTTCAGCACCTAGTGCAGTAATGGGTTGTCCAGGACACGATAACCGAGATTTTGAGTTT TGGCAAACAAATTGTCCTGGTGAACATATCAAGACGTGCATAGCACCTTTTTTTGACGAT GCTTCAAAAGTAACTGAACAGGAAAGACAAAGAATAATTGATACTGTCCCGTTCACATCT ACTGACGGTGTCCTAACCAAAGAATGCGGAGAACACTCAGGAGTTCTCACTGTAGTGGCA AGGAAATCGATAATGGGGATGTTGAATAGCGAAGGACTGTCTAAGAGCGTCGTTAGATAC AAAATTAGAGATTGGCTGATAAGTAGACAAAGATACTGGGGTACTCCAATACCCATCATT CACTGCGACAACTGTGGACCTGTCCCCGTTCCAGAAAGTGATCTACCTGTCAAGCTACCA GAACTTGAAGGCTTGGATACAAAAGGGAATCCGCTGTCCACAATCGATGAATTTGTAAAT GTCGCCTGTCCTTCATGCGGGAGCCCTGCGAAAAGAGAAACTGACACCATGGATACTTTT ATCGATAGTTCTTGGTATTATTTCAGATTCTTGGATCCCAAAAACACTTCAAAACCATTT GATCGTGAAATTGCAAGTAAAAATATGCCGGTTGATATTTATATTGGTGGAGTAGAACAT GCTATCTTACACTTGTTATACTCAAGGTTTATTGCTAAATTTCTCGGATCTATCAATGCA TGGAGCGACCCTGCTGGCATCTTCGAACCATTCAAAAAACTGGTGACACAAGGAATGGTT CAAGGGAAAACCTATGTTGACCCCGATTCTGGTAAATTTTTGAAGCCTGACGAGTTAACC TTTGTAAATGACTCTCCAGATGGCAACACAGTGATTATTAAATCAAATGGCAAGGTTCCG GTAGTCTCTTATGAAAAAATGTCGAAATCTAAATACAATGGTGCAGATCCAAATGAATGT ATTTTAAGACATGGACCTGATGCTACGAGAGCACATATCCTCTTCCAAAGTCCAATTGCA GATGCCCTGAATTGGGATGAATCCAAGATTGTTGGTATAGAACGTTGGTTGCAGAAGGTT CTTCATTTGACCAAAAATATTCTCAGTCTCGAGAAGGATTTGGCAATAAGTAAAGACTAC AAGACCCCGACCGACTTAAATGATGCAGAAGTGAAATTTCACAACGATTTCCAACGCTTC TTGAAATCAATTACGGAATCATTTGAAGTTAATCTATCGTTAAACACTGTAATATCTGAT TATATGAAGCTAACCAATATTTTAGAAAGTGCATTGAAAAAAGGTGAAGTAAGGAATGAA ATGATAGTACAGAATTTACAAAAGTTGGTAACCGTTATATATCCTGCTGTCCCGTCAATT TCAGAAGAAGCTGCAGAGATGATCAACTCCCAAATGGAATGGAACCAATACCGCTGGCCA GAAGTTGAGCGCACTACTGAGTCCAAATTCAAAAAGTTTCAAATTGTGGTAAATGGCAGA GTCAAATTCATGTACACGGCTGACAAAAACTTTTTGAAATTAGGTAGGGATGCTGTTATT GAAACTTTGATGAACTTACCGGAAGGGAGAATGTATTTGATGAATAAAAAAATCAAAAAA TTTGTCATGAAATTCAATGTGATTAGTTTCTTATTCCACAAGTAA
Protein Properties
Pfam Domain Function
Protein Residues894
Protein Molecular Weight101920.0
Protein Theoretical pI8.56
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Leucyl-tRNA synthetase, mitochondrial MLSRPSSRFLSTKRGPGPAVKKLIAIGEKWKQKTTRGLPKQDTLNSGSKYILCQFPYPSG ALHIGHLRVYVISDSLNRFYKQKGYNVIHPMGWDAFGLPAENAAIERSINPAIWTRDNIA KMKQQMQSMLANFDWDREITTCDPEYYKFTQWIFLKLFENGLAYRKEAEINWDPVDMTVL ANEQVDAQGRSWRSGAIVEKKQLKQWFLGITKFAPKLKKHLNQLKDWPSNVKQMQKNWIG ESVGAELVFKVADPKFENLIVFTTRPETLFAVQYVALALDHPIVQKYCEEMPDLKEFIQK SDQLPNDTKEGFQLPNIKAVNPLTKEEVPIFAAPYVVSSYGSAPSAVMGCPGHDNRDFEF WQTNCPGEHIKTCIAPFFDDASKVTEQERQRIIDTVPFTSTDGVLTKECGEHSGVLTVVA RKSIMGMLNSEGLSKSVVRYKIRDWLISRQRYWGTPIPIIHCDNCGPVPVPESDLPVKLP ELEGLDTKGNPLSTIDEFVNVACPSCGSPAKRETDTMDTFIDSSWYYFRFLDPKNTSKPF DREIASKNMPVDIYIGGVEHAILHLLYSRFIAKFLGSINAWSDPAGIFEPFKKLVTQGMV QGKTYVDPDSGKFLKPDELTFVNDSPDGNTVIIKSNGKVPVVSYEKMSKSKYNGADPNEC ILRHGPDATRAHILFQSPIADALNWDESKIVGIERWLQKVLHLTKNILSLEKDLAISKDY KTPTDLNDAEVKFHNDFQRFLKSITESFEVNLSLNTVISDYMKLTNILESALKKGEVRNE MIVQNLQKLVTVIYPAVPSISEEAAEMINSQMEWNQYRWPEVERTTESKFKKFQIVVNGR VKFMYTADKNFLKLGRDAVIETLMNLPEGRMYLMNKKIKKFVMKFNVISFLFHK
References
External Links
ResourceLink
Saccharomyces Genome Database NAM2
Uniprot IDP11325
Uniprot NameSYLM_YEAST
GenBank Gene IDJ03495
Genebank Protein ID172008
General Reference
  • Tzagoloff, A., Akai, A., Kurkulos, M., Repetto, B. (1988). "Homology of yeast mitochondrial leucyl-tRNA synthetase and isoleucyl- and methionyl-tRNA synthetases of Escherichia coli." J Biol Chem 263:850-856.2826465
  • Labouesse, M., Herbert, C. J., Dujardin, G., Slonimski, P. P. (1987). "Three suppressor mutations which cure a mitochondrial RNA maturase deficiency occur at the same codon in the open reading frame of the nuclear NAM2 gene." EMBO J 6:713-721.3034607
  • Johnston, M., Hillier, L., Riles, L., Albermann, K., Andre, B., Ansorge, W., Benes, V., Bruckner, M., Delius, H., Dubois, E., Dusterhoft, A., Entian, K. D., Floeth, M., Goffeau, A., Hebling, U., Heumann, K., Heuss-Neitzel, D., Hilbert, H., Hilger, F., Kleine, K., Kotter, P., Louis, E. J., Messenguy, F., Mewes, H. W., Hoheisel, J. D., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Nature 387:87-90.9169871
  • Zagorski, W., Castaing, B., Herbert, C. J., Labouesse, M., Martin, R., Slonimski, P. P. (1991). "Purification and characterization of the Saccharomyces cerevisiae mitochondrial leucyl-tRNA synthetase." J Biol Chem 266:2537-2541.1990003
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106