Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial (P07275)

Identification

Name

Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial

Synonyms

P5C dehydrogenase

Gene Name

PUT2

Enzyme Class

1.5.1.12

Biological Properties

General Function

Involved in oxidoreductase activity

Specific Function

(S)-1-pyrroline-5-carboxylate + NAD(P)(+) + 2 H(2)O = L-glutamate + NAD(P)H

Cellular Location

Mitochondrion inner membrane

SMPDB Pathways

4-aminobutanoate degradation

PW002382

KEGG Pathways

Alanine, aspartate and glutamate metabolism	ec00250
Arginine and proline metabolism	ec00330

SMPDB Reactions

Succinic acid semialdehyde + NAD + water → Succinic acid + NADH + hydron

KEGG Reactions

Metabolites

YMDB ID	Name	View
YMDB00110	NAD	Show
YMDB00143	NADH	Show
YMDB00236	Succinic acid semialdehyde	Show
YMDB00243	1-Pyrroline-5-carboxylic acid	Show
YMDB00271	L-Glutamic acid	Show
YMDB00338	Succinic acid	Show
YMDB00412	NAD(+)	Show
YMDB00426	NADPH	Show
YMDB00427	NADP	Show
YMDB00718	1-pyrroline-3-hydroxy-5-carboxylic acid	Show
YMDB00719	erythro-4-hydroxy-L-glutamic acid	Show
YMDB00720	L-4-hydroxyglutamic semialdehyde	Show
YMDB00723	trans-4-hydroxy-L-proline	Show
YMDB00862	hydron	Show
YMDB00890	water	Show
YMDB00918	l-Glutamic-gamma-semialdehyde	Show

GO Classification

Component
cytoplasmic part
mitochondrial part
mitochondrial matrix
cell part
intracellular part
Function
oxidoreductase activity, acting on the CH-NH group of donors
oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor
1-pyrroline-5-carboxylate dehydrogenase activity
catalytic activity
oxidoreductase activity
Process
oxidation reduction
proline metabolic process
proline biosynthetic process
metabolic process
cellular metabolic process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
glutamine family amino acid metabolic process

Gene Properties

Chromosome Location

chromosome 8

Locus

YHR037W

Gene Sequence

>1728 bp ATGCTATCAGCAAGGTGCCTCAAATCTATATACTTCAAGAGATCTTTCTCACAACTGGGA CACATCAAGCCCCCCAAGCACATAAGAAATGAACCTGTTAAGCCATTTAGAAACATAGAC TTAAAAGACTGGGACTTACTGAGGGCTTCCTTGATGAAATTCAAAAGTTCTTCTTTAGAA GTGCCACTGGTCATCAATGGGGAAAGGATATATGACAATAATGAAAGAGCGCTTTTCCCG CAGACTAACCCTGCGAACCATCAACAAGTACTGGCAAACGTCACACAAGCCACGGAAAAA GATGTCATGAATGCTGTAAAAGCCGCCAAGGATGCCAAAAAGGATTGGTACAATCTACCG TTTTATGACAGATCTGCAATTTTTTTGAAAGCCGCTGACTTAATTTCCACCAAGTATCGT TATGACATGTTAGCTGCTACAATGCTGGGCCAGGGAAAAAATGTGTATCAGGCAGAAATC GACTGTATCACGGAATTATCTGACTTTTTTAGATACTATGTCAAGTATGCATCAGACTTG TATGCTCAACAACCCGTGAGTCGAGCGGATGGTACTTGGAATAAAGCTGAATACAGACCT TTAGAGGGATTTGTGTATGCTGTTTCACCATTCAACTTTACTGCTATTGCTGCAAACTTG ATTGGGGCTCCAGCTTTGATGGGTAATACAGTTGTCTGGAAACCTTCACAAACCGCTGCC CTTTCAAATTACTTATTGATGACTGTTTTGGAAGAAGCGGGATTGCCAAAGGGTGTCATA AATTTCATTCTAGGTGATCCAGTTCAAGTTACTGACCAGGTATTAGCTGATAAAGATTTT GGTGCCTTGCATTTTACCGGTTCTACAAATGTCTTTAAGAGTTTGTATGGCAAAATACAA AGTGGCGTTGTTGAAGGGAAGTACAGAGATTACCCCCGTATTATTGGTGAGACAGGTGGT AAAAATTTCCATCTAGTTCACCCAAGTGCAAATATATCACATGCAGTACTCTCTACTATT AGAGGCACTTTCGAGTTCCAAGGCCAAAAGTGCTCTGCCGCTTCTAGGTTATATCTTCCA GAGTCAAAAAGTGAAGAATTCTTATCCGATATGTTTGGCATATTGCAGTCACAGAATGTT GTCCCAATGAACACATCCGCAAGTCCAATTTCTGGTGGGAATTTGCGGGGATTTATGGGT CCTGTCATCCATGAACAAAGTTTCGACAAATTAGTTAAAGTAATTGAAGATGCAAAGAAA GACCCCGAGTTGGAAATTCTTTACGGTGGACAATACGATAAAAGCCAAGGTTGGTTTGTC GGACCCACAGTCATAAAAGCCAAGAGACCAGATCATCCATATATGTCAACAGAATTTTTT GGCCCTATATTAACTGTTTACGAGTATCCAGATACGGAATTTAATGAAATCTGTGATATT ATCGATAATACGAGTCAATACGCCTTAACTGGTGCTATTTTTGCCAAAGATCGTAAAGCA ATTGAATACGCAGATGAGAAGTTGAAGTTTAGCGCAGGAAATTTCTACATAAATGATAAG TGTACTGGTGCCGTTGTTTCTCAGCAATGGTTTGGTGGCGCAAGAATGAGTGGTACCGAC GGTAAGGCTGGTGGTCCAAACATTTTAAGCAGATTTGTCAGTATTAGAAACACAAAGGAG AGCTTCTACGAGTTGACTGATTTCAAATATCCATCGAATTATGAATAA

Protein Properties

Pfam Domain Function

Aldedh (PF00171 )

Protein Residues

575

Protein Molecular Weight

64434.60156

Protein Theoretical pI

7.0

Signalling Regions

None

Transmembrane Regions

None

Protein Sequence

>Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial MLSARCLKSIYFKRSFSQLGHIKPPKHIRNEPVKPFRNIDLKDWDLLRASLMKFKSSSLE VPLVINGERIYDNNERALFPQTNPANHQQVLANVTQATEKDVMNAVKAAKDAKKDWYNLP FYDRSAIFLKAADLISTKYRYDMLAATMLGQGKNVYQAEIDCITELSDFFRYYVKYASDL YAQQPVESADGTWNKAEYRPLEGFVYAVSPFNFTAIAANLIGAPALMGNTVVWKPSQTAA LSNYLLMTVLEEAGLPKGVINFIPGDPVQVTDQVLADKDFGALHFTGSTNVFKSLYGKIQ SGVVEGKYRDYPRIIGETGGKNFHLVHPSANISHAVLSTIRGTFEFQGQKCSAASRLYLP ESKSEEFLSDMFGILQSQNVVPMNTSASPISGGNLRGFMGPVIHEQSFDKLVKVIEDAKK DPELEILYGGQYDKSQGWFVGPTVIKAKRPDHPYMSTEFFGPILTVYEYPDTEFNEICDI IDNTSQYALTGAIFAKDRKAIEYADEKLKFSAGNFYINDKCTGAVVSQQWFGGARMSGTD DKAGGPNILSRFVSIRNTKENFYELTDFKYPSNYE

References

External Links

Resource	Link
Saccharomyces Genome Database	PUT2
Uniprot ID	P07275
Uniprot Name	PUT2_YEAST
GenBank Gene ID	M10029
Genebank Protein ID	172303

General Reference

Krzywicki, K. A., Brandriss, M. C. (1984). "Primary structure of the nuclear PUT2 gene involved in the mitochondrial pathway for proline utilization in Saccharomyces cerevisiae." Mol Cell Biol 4:2837-2842.6098824
Johnston, M., Andrews, S., Brinkman, R., Cooper, J., Ding, H., Dover, J., Du, Z., Favello, A., Fulton, L., Gattung, S., et, a. l. .. (1994). "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Science 265:2077-2082.8091229
Siddiqui, A. H., Brandriss, M. C. (1988). "A regulatory region responsible for proline-specific induction of the yeast PUT2 gene is adjacent to its TATA box." Mol Cell Biol 8:4634-4641.3062363
Grandier-Vazeille, X., Bathany, K., Chaignepain, S., Camougrand, N., Manon, S., Schmitter, J. M. (2001). "Yeast mitochondrial dehydrogenases are associated in a supramolecular complex." Biochemistry 40:9758-9769.11502169
Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106