NAD-dependent histone deacetylase SIR2 (P06700)

Identification
NameNAD-dependent histone deacetylase SIR2
Synonyms
  • Regulatory protein SIR2
  • Silent information regulator 2
Gene NameSIR2
Enzyme ClassNot Available
Biological Properties
General FunctionTranscription
Specific FunctionNAD-dependent deacetylase, which participates in a wide range of cellular events including chromosome silencing, chromosome segregation, DNA recombination and the determination of life span. Involved in transcriptional repression of the silent mating-type loci HML and HMR and telomeric silencing via its association with SIR3 and SIR4. Plays a central role in ribosomal DNA (rDNA) silencing via its association with the RENT complex, preventing hyperrecombination, and repressing transcription from foreign promoters, which contributes to extending life span. Probably represses transcription via the formation of heterochromatin structure, which involves the compaction of chromatin fiber into a more condensed form, although this complex in at least one case can still bind euchromatic levels of positive transcription regulators. Although it displays some NAD-dependent histone deacetylase activity on 'Lys-9' and 'Lys-14' of histone H3 and 'Lys-16' of histone H4 in vitro, such activity is unclear in vivo and may not be essential
Cellular LocationNucleus, nucleolus.
SMPDB Pathways
NAD metabolismPW002421 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG PathwaysNot Available
SMPDB Reactions
NAD + water + [histone]-N6-acetyl-L-lysine → 3''-O-acetyl-ADP-ribose + a [histone]-L-lysine + Niacinamide
KEGG Reactions
NAD + water ↔ hydron + Niacinamide + Adenosine diphosphate ribose
Metabolites
YMDB IDNameView
YMDB00110NADShow
YMDB00274NiacinamideShow
YMDB00890waterShow
YMDB163063''-O-acetyl-ADP-riboseShow
GO Classification
Function
NAD or NADH binding
NAD-dependent histone deacetylase activity
hydrolase activity
binding
ion binding
cation binding
metal ion binding
transition metal ion binding
zinc ion binding
catalytic activity
NAD binding
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
nucleotide binding
histone deacetylase activity
Process
regulation of transcription, DNA-dependent
biological regulation
metabolic process
regulation of biological process
regulation of metabolic process
regulation of macromolecule metabolic process
regulation of gene expression
regulation of transcription
cellular process
gene silencing
chromatin silencing
macromolecule metabolic process
macromolecule modification
protein modification process
post-translational protein modification
protein amino acid deacetylation
Gene Properties
Chromosome LocationNot Available
Locus
Gene Sequence>1689 bp ATGACCATCCCACATATGAAATACGCCGTATCAAAGACTAGCGAAAATAAGGTTTCAAAT ACAGTAAGCCCCACACAAGATAAAGACGCGATCAGAAAACAACCCGATGACATTATAAAT AATGATGAACCTTCACATAAGAAGATAAAAGTAGCACAGCCGGATTCCTTGAGGGAAACC AACACAACAGATCCACTTGGGCACACTAAAGCTGCGCTCGGAGAAGTGGCATCGATGGAG CTCAAACCAACTAATGACATGGATCCCTTGGCAGTGTCAGCAGCTTCAGTAGTGTCAATG TCCAATGACGTTTTGAAACCAGAGACGCCCAAGGGGCCAATCATAATCAGTAAAAACCCA TCAAATGGTATTTTCTATGGTCCCTCCTTCACTAAACGAGAGTCTCTCAATGCTCGAATG TTTCTGAAATACTATGGTGCACACAAATTTTTAGACACTTACCTCCCCGAGGATTTGAAC TCGTTATACATTTACTATCTTATCAAGTTGCTAGGCTTTGAAGTTAAAGATCAAGCGCTT ATCGGCACCATCAACAGTATTGTCCATATCAACTCGCAAGAGCGTGTTCAAGATTTGGGA AGTGCAATATCTGTCACAAATGTTGAAGACCCATTGGCAAAAAAGCAAACAGTTCGTCTA ATCAAAGATTTGCAAAGAGCAATTAACAAAGTTCTATGTACAAGATTAAGATTATCCAAT TTTTTCACTATTGATCATTTTATTCAAAAATTACATACCGCTAGAAAAATTTTGGTCCTG ACTGGTGCAGGTGTTTCAACTTCATTAGGGATCCCGGACTTCAGATCTTCTGAGGGGTTC TATTCAAAGATCAAACATTTGGGGCTCGATGATCCCCAAGACGTTTTCAATTACAATATA TTTATGCACGACCCCTCTGTTTTCTATAATATTGCCAATATGGTTTTACCTCCAGAAAAA ATTTATTCTCCATTGCATAGTTTCATTAAGATGCTACAAATGAAAGGGAAATTATTGAGA AATTATACTCAAAACATTGATAATTTGGAATCTTATGCGGGAATAAGCACAGATAAACTG GTGCAGTGCCATGGCTCTTTTGCTACTGCCACCTGCGTTACCTGCCATTGGAACCTACCC GGTGAGAGGATATTTAATAAAATTAGAAACCTCGAACTTCCACTATGCCCGTACTGTTAC AAAAAAAGAAGAGAATATTTCCCAGAGGGATATAATAATAAAGTAGGTGTTGCTGCATCA CAGGGTTCAATGTCGGAAAGGCCTCCATATATCCTTAACTCATATGGCGTTCTCAAACCA GATATCACATTCTTTGGCGAAGCACTGCCAAATAAATTTCATAAGAGCATTCGCGAAGAT ATCTTAGAATGTGATTTGTTGATTTGCATTGGGACAAGTTTAAAAGTAGCGCCAGTGTCT GAAATCGTAAACATGGTTCCTTCCCACGTTCCCCAAGTCCTGATTAATCGTGATCCCGTC AAGCACGCAGAATTTGATTTATCTCTTTTGGGGTACTGTGATGACATTGCAGCTATGGTA GCCCAAAAATGTGGCTGGACGATTCCGCATAAGAAATGGAACGATTTGAAGAACAAGAAC TTTAAATGCCAAGAGAAGGATAAGGGCGTGTATGTCGTTACATCAGATGAACATCCCAAA ACCCTCTAA
Protein Properties
Pfam Domain Function
Protein Residues562
Protein Molecular Weight63261.30078
Protein Theoretical pI8.66
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>NAD-dependent histone deacetylase SIR2 MTIPHMKYAVSKTSENKVSNTVSPTQDKDAIRKQPDDIINNDEPSHKKIKVAQPDSLRET NTTDPLGHTKAALGEVASMELKPTNDMDPLAVSAASVVSMSNDVLKPETPKGPIIISKNP SNGIFYGPSFTKRESLNARMFLKYYGAHKFLDTYLPEDLNSLYIYYLIKLLGFEVKDQAL IGTINSIVHINSQERVQDLGSAISVTNVEDPLAKKQTVRLIKDLQRAINKVLCTRLRLSN FFTIDHFIQKLHTARKILVLTGAGVSTSLGIPDFRSSEGFYSKIKHLGLDDPQDVFNYNI FMHDPSVFYNIANMVLPPEKIYSPLHSFIKMLQMKGKLLRNYTQNIDNLESYAGISTDKL VQCHGSFATATCVTCHWNLPGERIFNKIRNLELPLCPYCYKKRREYFPEGYNNKVGVAAS QGSMSERPPYILNSYGVLKPDITFFGEALPNKFHKSIREDILECDLLICIGTSLKVAPVS EIVNMVPSHVPQVLINRDPVKHAEFDLSLLGYCDDIAAMVAQKCGWTIPHKKWNDLKNKN FKCQEKDKGVYVVTSDEHPKTL
References
External Links
ResourceLink
Saccharomyces Genome Database SIR2
Uniprot IDP06700
Uniprot NameSIR2_YEAST
GenBank Gene IDX01419
Genebank Protein ID4470
General Reference
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