Geranylgeranyl pyrophosphate synthase (Q12051)

Identification
NameGeranylgeranyl pyrophosphate synthase
Synonyms
  • GGPP synthase
  • GGPPSase
  • BET2 suppressor protein 1
  • Geranylgeranyl diphosphate synthase
  • Dimethylallyltranstransferase
  • Geranyltranstransferase
  • Farnesyltranstransferase
Gene NameBTS1
Enzyme Class
Biological Properties
General FunctionInvolved in isoprenoid biosynthetic process
Specific FunctionCatalyzes the trans-addition of the 3 molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate. Required for the membrane attachment of YPT1 and SEC4. May be involved in vesicle trafficking and protein sorting
Cellular LocationCytoplasm
SMPDB Pathways
Terpenoid backbone biosynthesisPW002485 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
Terpenoid backbone biosynthesisec00900 Map00900
SMPDB ReactionsNot Available
KEGG Reactions
2-trans,6-trans-farnesyl diphosphate + Isopentenyl pyrophosphate → Pyrophosphate + 2-trans,6-trans,10-trans-geranylgeranyl diphosphate
Metabolites
YMDB IDNameView
YMDB00095DimethylallylpyrophosphateShow
YMDB00200Isopentenyl pyrophosphateShow
YMDB00219PyrophosphateShow
YMDB00266Geranyl-PPShow
YMDB003252-trans,6-trans-farnesyl diphosphateShow
YMDB003592-trans,6-trans,10-trans-geranylgeranyl diphosphateShow
YMDB00631geranylgeranyl diphosphateShow
GO Classification
Component
Not Available
Function
Not Available
Process
cellular lipid metabolic process
isoprenoid metabolic process
isoprenoid biosynthetic process
metabolic process
primary metabolic process
lipid metabolic process
Gene Properties
Chromosome Locationchromosome 16
LocusYPL069C
Gene Sequence>1008 bp ATGGAGGCCAAGATAGATGAGCTGATCAATAATGATCCTGTTTGGTCCAGCCAAAATGAA AGCTTGATTTCAAAACCTTATAATCACATCCTTTTGAAACCTGGCAAGAACTTTAGACTA AATTTAATAGTTCAAATTAACAGAGTTATGAATTTGCCCAAAGACCAGCTGGCCATAGTT TCGCAAATTGTTGAGCTCTTGCATAATTCCAGCCTTTTAATCGACGATATAGAAGATAAT GCTCCCTTGAGAAGGGGACAGACCACTTCTCACTTAATCTTCGGTGTACCCTCCACTATA AACACCGCAAATTATATGTATTTCAGAGCCATGCAACTTGTATCGCAGCTAACCACAAAA GAGCCTTTGTATCATAATTTGATTACGATTTTCAACGAAGAATTGATCAATCTACATAGG GGACAAGGCTTGGATATATACTGGAGAGACTTTCTGCCTGAAATCATACCTACTCAGGAG ATGTATTTGAATATGGTTATGAATAAAACAGGCGGCCTTTTCAGATTAACGTTGAGACTC ATGGAAGCGCTGTCTCCTTCCTCACACCACGGCCATTCGTTGGTTCCTTTCATAAATCTT CTGGGTATTATTTATCAGATTAGAGATGATTACTTGAATTTGAAAGATTTCCAAATGTCC AGCGAAAAAGGCTTTGCTGAGGACATTACAGAGGGGAAGTTATCTTTTCCCATCGTCCAC GCCCTTAACTTCACTAAAACGAAAGGTCAAACTGAGCAACACAATGAAATTCTAAGAATT CTCCTGTTGAGGACAAGTGATAAAGATATAAAACTAAAGCTGATTCAAATACTGGAATTC GACACCAATTCATTGGCCTACACCAAAAATTTTATTAATCAATTAGTGAATATGATAAAA AATGATAATGAAAATAAGTATTTACCTGATTTGGCTTCGCATTCCGACACCGCCACCAAT TTACATGACGAATTGTTATATATAATAGACCACTTATCCGAATTGTGA
Protein Properties
Pfam Domain Function
Protein Residues335
Protein Molecular Weight38651.0
Protein Theoretical pI6.04
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Geranylgeranyl pyrophosphate synthase MEAKIDELINNDPVWSSQNESLISKPYNHILLKPGKNFRLNLIVQINRVMNLPKDQLAIV SQIVELLHNSSLLIDDIEDNAPLRRGQTTSHLIFGVPSTINTANYMYFRAMQLVSQLTTK EPLYHNLITIFNEELINLHRGQGLDIYWRDFLPEIIPTQEMYLNMVMNKTGGLFRLTLRL MEALSPSSHHGHSLVPFINLLGIIYQIRDDYLNLKDFQMSSEKGFAEDITEGKLSFPIVH ALNFTKTKGQTEQHNEILRILLLRTSDKDIKLKLIQILEFDTNSLAYTKNFINQLVNMIK NDNENKYLPDLASHSDTATNLHDELLYIIDHLSEL
References
External Links
ResourceLink
Saccharomyces Genome Database BTS1
Uniprot IDQ12051
Uniprot NameGGPPS_YEAST
GenBank Gene IDAY692852
Genebank Protein ID51013155
General Reference
  • Jiang, Y., Proteau, P., Poulter, D., Ferro-Novick, S. (1995). "BTS1 encodes a geranylgeranyl diphosphate synthase in Saccharomyces cerevisiae." J Biol Chem 270:21793-21799.7665600
  • Bussey, H., Storms, R. K., Ahmed, A., Albermann, K., Allen, E., Ansorge, W., Araujo, R., Aparicio, A., Barrell, B., Badcock, K., Benes, V., Botstein, D., Bowman, S., Bruckner, M., Carpenter, J., Cherry, J. M., Chung, E., Churcher, C., Coster, F., Davis, K., Davis, R. W., Dietrich, F. S., Delius, H., DiPaolo, T., Hani, J., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Nature 387:103-105.9169875
  • Hu, Y., Rolfs, A., Bhullar, B., Murthy, T. V., Zhu, C., Berger, M. F., Camargo, A. A., Kelley, F., McCarron, S., Jepson, D., Richardson, A., Raphael, J., Moreira, D., Taycher, E., Zuo, D., Mohr, S., Kane, M. F., Williamson, J., Simpson, A., Bulyk, M. L., Harlow, E., Marsischky, G., Kolodner, R. D., LaBaer, J. (2007). "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Genome Res 17:536-543.17322287
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Shiflett, S. L., Vaughn, M. B., Huynh, D., Kaplan, J., Ward, D. M. (2004). "Bph1p, the Saccharomyces cerevisiae homologue of CHS1/beige, functions in cell wall formation and protein sorting." Traffic 5:700-710.15296494
  • Lo, C. H., Chang, Y. H., Wright, J. D., Chen, S. H., Kan, D., Lim, C., Liang, P. H. (2009). "Combined experimental and theoretical study of long-range interactions modulating dimerization and activity of yeast geranylgeranyl diphosphate synthase." J Am Chem Soc 131:4051-4062.19245203
  • Chang, T. H., Guo, R. T., Ko, T. P., Wang, A. H., Liang, P. H. (2006). "Crystal structure of type-III geranylgeranyl pyrophosphate synthase from Saccharomyces cerevisiae and the mechanism of product chain length determination." J Biol Chem 281:14991-15000.16554305
  • Guo, R. T., Cao, R., Liang, P. H., Ko, T. P., Chang, T. H., Hudock, M. P., Jeng, W. Y., Chen, C. K., Zhang, Y., Song, Y., Kuo, C. J., Yin, F., Oldfield, E., Wang, A. H. (2007). "Bisphosphonates target multiple sites in both cis- and trans-prenyltransferases." Proc Natl Acad Sci U S A 104:10022-10027.17535895